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The map label for this gene is lacI [H]

Identifier: 50843499

GI number: 50843499

Start: 2232222

End: 2233223

Strand: Reverse

Name: lacI [H]

Synonym: PPA2055

Alternate gene names: 50843499

Gene position: 2233223-2232222 (Counterclockwise)

Preceding gene: 50843500

Following gene: 50843498

Centisome position: 87.23

GC content: 65.17

Gene sequence:

>1002_bases
ATGGTGAGGCGGCCCTGGTGGCCTCCGCGGTTGGCTGACGTCGCCGAGCGCTCCGGCGTCTCGTTGGCAACGGCGTCGCG
TGCCTTGGCACGTCAGAATGGCGCTGAAGGTGCCCTCCCGCAGGCCACCGAGGAGATCCTCACCGCGGCCACCGAGCTGG
GATATTGGCGCACCGACCCTGACGTCGCCCGGCTGCACGTTCTCGTCTCCGACATCGGCCGCACCGGCTACTGGGCGACG
CTCTCGGGGGTACTCAGCGCAGCCCTCGACCTCGGTGCCGAGACCTCCCTACATGTATTGGCGGGGTCGCCGGAGCGATG
CCGTTCCACCGTGCAATGTGCCTTAGACGCTCGTGCCGACGGGGTTGTCGTCCTTGAATTTGATTCCCCCAGCGTGGCCC
TCCTGCCTGGGTTGCCCGTCGATCTCCCAGTTGCCATTGCCGGAGGCAAACCCGAGGGAGGCGAGCCCTGGTCTCGGGCG
TGGATCGATGATCATGCCGGTGCAGTAATGGCCACCGAATACTTGGCGGGTTTGGGCCACCGCCGCATCGCCTATGTCGG
AGTTCCTCCTGCAGGGCACCCTGATCCACGATGTGCGGGATGGCGCGAAGTCCTGGCTACTCGAGGTCTCCCGCCGCTCG
AACCATTGGCGACTGGGTGGTCAGTCGAGACGGGTATGCACGTTGCACGGGCGGTACGAGCGCACCAGGTGACCGCAGTC
CTATGTGGAAACGACGATGTGGCTCTCGGCCTCTTAACAGGGCTTGACAGGTTGGGGTTGAGAGTTCCCGAGGATGTCTC
GGTCGTCGGCATGGATGATCATCCCCATTCGGTGGCCACTCGGCCAGCGCTCACGACGGTGCGGCTTGATTTCGCCGCCG
TGGGAGAAACTGCGGCCCGTCTTGCCCTTGGAACGGATCCCAGTGGAACAGTCGAGATTCCTACGACCCTCATCACCCGA
GCCTCAGCGTGTCCCCCGGTGGATGGCCGCGCCGATCCTTGA

Upstream 100 bases:

>100_bases
GCGGATGCGGCCCCGTCGCGCCCTGGTCTTCCGCGGACGCCCTTGCCGAGGACAGGGGTGTGAACCCGGTATCGTCCGGT
GCATGGAACGTGACCCGGCC

Downstream 100 bases:

>100_bases
CGTCGGCATGATGGGACCATGACTATGGATCCACTGACCGCCCTGGAAACCTCCACCCGTATCGCCGCTGGTGACGTCGG
GATCCTTGACATTGTCGACG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVRRPWWPPRLADVAERSGVSLATASRALARQNGAEGALPQATEEILTAATELGYWRTDPDVARLHVLVSDIGRTGYWAT
LSGVLSAALDLGAETSLHVLAGSPERCRSTVQCALDARADGVVVLEFDSPSVALLPGLPVDLPVAIAGGKPEGGEPWSRA
WIDDHAGAVMATEYLAGLGHRRIAYVGVPPAGHPDPRCAGWREVLATRGLPPLEPLATGWSVETGMHVARAVRAHQVTAV
LCGNDDVALGLLTGLDRLGLRVPEDVSVVGMDDHPHSVATRPALTTVRLDFAAVGETAARLALGTDPSGTVEIPTTLITR
ASACPPVDGRADP

Sequences:

>Translated_333_residues
MVRRPWWPPRLADVAERSGVSLATASRALARQNGAEGALPQATEEILTAATELGYWRTDPDVARLHVLVSDIGRTGYWAT
LSGVLSAALDLGAETSLHVLAGSPERCRSTVQCALDARADGVVVLEFDSPSVALLPGLPVDLPVAIAGGKPEGGEPWSRA
WIDDHAGAVMATEYLAGLGHRRIAYVGVPPAGHPDPRCAGWREVLATRGLPPLEPLATGWSVETGMHVARAVRAHQVTAV
LCGNDDVALGLLTGLDRLGLRVPEDVSVVGMDDHPHSVATRPALTTVRLDFAAVGETAARLALGTDPSGTVEIPTTLITR
ASACPPVDGRADP
>Mature_333_residues
MVRRPWWPPRLADVAERSGVSLATASRALARQNGAEGALPQATEEILTAATELGYWRTDPDVARLHVLVSDIGRTGYWAT
LSGVLSAALDLGAETSLHVLAGSPERCRSTVQCALDARADGVVVLEFDSPSVALLPGLPVDLPVAIAGGKPEGGEPWSRA
WIDDHAGAVMATEYLAGLGHRRIAYVGVPPAGHPDPRCAGWREVLATRGLPPLEPLATGWSVETGMHVARAVRAHQVTAV
LCGNDDVALGLLTGLDRLGLRVPEDVSVVGMDDHPHSVATRPALTTVRLDFAAVGETAARLALGTDPSGTVEIPTTLITR
ASACPPVDGRADP

Specific function: Repressor of the lactose operon. Binds allolactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=350, Percent_Identity=27.4285714285714, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=30.7926829268293, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=26.5671641791045, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789068, Length=348, Percent_Identity=27.2988505747126, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=25.748502994012, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994940, Length=293, Percent_Identity=27.3037542662116, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=28.1899109792285, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=27.6450511945392, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34829; Mature: 34829

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00356 HTH_LACI_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRPWWPPRLADVAERSGVSLATASRALARQNGAEGALPQATEEILTAATELGYWRTDP
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DVARLHVLVSDIGRTGYWATLSGVLSAALDLGAETSLHVLAGSPERCRSTVQCALDARAD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCC
GVVVLEFDSPSVALLPGLPVDLPVAIAGGKPEGGEPWSRAWIDDHAGAVMATEYLAGLGH
CEEEEEECCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCC
RRIAYVGVPPAGHPDPRCAGWREVLATRGLPPLEPLATGWSVETGMHVARAVRAHQVTAV
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHEEEEE
LCGNDDVALGLLTGLDRLGLRVPEDVSVVGMDDHPHSVATRPALTTVRLDFAAVGETAAR
EECCCCEEHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHCCCCEEEEEEHHHHHCHHHHH
LALGTDPSGTVEIPTTLITRASACPPVDGRADP
EEECCCCCCCEECCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVRRPWWPPRLADVAERSGVSLATASRALARQNGAEGALPQATEEILTAATELGYWRTDP
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DVARLHVLVSDIGRTGYWATLSGVLSAALDLGAETSLHVLAGSPERCRSTVQCALDARAD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCC
GVVVLEFDSPSVALLPGLPVDLPVAIAGGKPEGGEPWSRAWIDDHAGAVMATEYLAGLGH
CEEEEEECCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCC
RRIAYVGVPPAGHPDPRCAGWREVLATRGLPPLEPLATGWSVETGMHVARAVRAHQVTAV
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHEEEEE
LCGNDDVALGLLTGLDRLGLRVPEDVSVVGMDDHPHSVATRPALTTVRLDFAAVGETAAR
EECCCCEEHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHCCCCEEEEEEHHHHHCHHHHH
LALGTDPSGTVEIPTTLITRASACPPVDGRADP
EEECCCCCCCEECCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 355891; 9278503; 1107032; 4571224; 4594037; 3286877; 7498473; 2178920; 8046748; 2040302; 1923807; 3064080; 2742823; 8683581; 10647179; 8638105 [H]