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The map label for this gene is 50843498

Identifier: 50843498

GI number: 50843498

Start: 2230797

End: 2232197

Strand: Reverse

Name: 50843498

Synonym: PPA2054

Alternate gene names: NA

Gene position: 2232197-2230797 (Counterclockwise)

Preceding gene: 50843499

Following gene: 50843493

Centisome position: 87.19

GC content: 67.09

Gene sequence:

>1401_bases
ATGGATCCACTGACCGCCCTGGAAACCTCCACCCGTATCGCCGCTGGTGACGTCGGGATCCTTGACATTGTCGACGCCGC
CCTCGAGGCGGCACATGCCGACGAATGTCACGCTTGGACCTGGATCGATGACACCGGGGCTCGGAACACCGCCCGAAAAC
TCCAGCGTCAACTCGATGCCGGACAAGCCCGTCCCGGCCCGCTGTTCGGTGTTCCCTGCCCGGTCAAGGACCTCAACGCC
TTAGCCGGGGCGCCCCGAGCCGTCGGTAGCCGAGCTGTCGGCGGGACGCCTTCGGACCACGACGACGGCATCGTCACTCG
ACTCAAGGCCGCCGGTGCCATCCCGATCGGTACGACGGCCGCTCCCGAGTTTGGGCTTCCCTGCTACACCGAGCCGGATG
GTCGGGCGGCGACCGTCACTCCGTGGGACCACACCCGAATGGCGGGAGGGTCCTCTGGTGGTGCTGCGGCCGCAGTCGGG
GCTGGTCAGGTGCCGATCGCTCAAGGCTCCGACGGCGGCGGGTCGATACGCATCCCGGCGTCATGTTGCGGTGTCGTCGG
CCTCAAACCCAGCCGTGGGCGAGTTTCGGCCGGTCCTTACGGGATCGACGGTCCCGGGCTGGTCTGCGGTGGGGTGCTGA
CCCGCACGGTGCGCGACACCGCAGCCGCTCTTGACGTGCTGTCCGGGCCGTGGCCGGGAGACCCCTTCTACGCCCCGACA
CCGACCGAGACCTTCTTGTCGGCCTGTGGCCGTGATCCGGGACGGCTGCGCATCGGAATTCTGCGCGAACCGGTTATCGC
TGACGCCCAGGTCCATCCGGACATCCTGGCCGCGCTGGACCGGACAGCCGCCACCTTGGAGTCCATGGGCCACGTCGTCG
AGCAGACGAGTGCTCCGTTCGGCCCGCGTGAGTGGGACTCTTTTCGGTGCGTGTGGGAAGTGGGGGCGGCCGCGATCCCG
GTCTTGCCCGAACGCGAGGAGCTCTGCACACCCATGTCTCGGTGGCTGCGGGCCGGGGGCCGACGTCGCAGCGGAGTCGA
GCTGGCCGAGGCGTTAGCCGGTATCCAGATGGTGGCCCGTCAAGTCGGGCAGCATTGGCGGCGGTTCGACGTCATTGTTA
GCCCGACCTTGGCTCAGCCTCCTTTGCCAGTTGGGGCTTTGCGGGACGATGAGGATCCGGCGGCCGATTTTGACGCCCAG
TGTCGCTTCACACCGTGGACCAGCTTGTGGAATATCACCGGGTGCCCGGCGATGAGCCTGCCGCTGCACAACACGAGGAT
CGATGGTGTCGAGCTGCCGATCGGCATCATGATTGGCGGTCGGCTGTACGACGAGGCCACCCTGCTGGCACTCGGATGTG
CCCTAGAAAATACTCGGGACTGCGAACGCAGCTGCCGATGA

Upstream 100 bases:

>100_bases
AACAGTCGAGATTCCTACGACCCTCATCACCCGAGCCTCAGCGTGTCCCCCGGTGGATGGCCGCGCCGATCCTTGACGTC
GGCATGATGGGACCATGACT

Downstream 100 bases:

>100_bases
TCAAAACTCCCGCCTCACCTGCGGTGACAGCACTGACATGCCTGCCCACGGGCCCGGGCGGGAGTTTTTGCATGTCGACC
TGTGCGCAGCCACGGCCGCG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MDPLTALETSTRIAAGDVGILDIVDAALEAAHADECHAWTWIDDTGARNTARKLQRQLDAGQARPGPLFGVPCPVKDLNA
LAGAPRAVGSRAVGGTPSDHDDGIVTRLKAAGAIPIGTTAAPEFGLPCYTEPDGRAATVTPWDHTRMAGGSSGGAAAAVG
AGQVPIAQGSDGGGSIRIPASCCGVVGLKPSRGRVSAGPYGIDGPGLVCGGVLTRTVRDTAAALDVLSGPWPGDPFYAPT
PTETFLSACGRDPGRLRIGILREPVIADAQVHPDILAALDRTAATLESMGHVVEQTSAPFGPREWDSFRCVWEVGAAAIP
VLPEREELCTPMSRWLRAGGRRRSGVELAEALAGIQMVARQVGQHWRRFDVIVSPTLAQPPLPVGALRDDEDPAADFDAQ
CRFTPWTSLWNITGCPAMSLPLHNTRIDGVELPIGIMIGGRLYDEATLLALGCALENTRDCERSCR

Sequences:

>Translated_466_residues
MDPLTALETSTRIAAGDVGILDIVDAALEAAHADECHAWTWIDDTGARNTARKLQRQLDAGQARPGPLFGVPCPVKDLNA
LAGAPRAVGSRAVGGTPSDHDDGIVTRLKAAGAIPIGTTAAPEFGLPCYTEPDGRAATVTPWDHTRMAGGSSGGAAAAVG
AGQVPIAQGSDGGGSIRIPASCCGVVGLKPSRGRVSAGPYGIDGPGLVCGGVLTRTVRDTAAALDVLSGPWPGDPFYAPT
PTETFLSACGRDPGRLRIGILREPVIADAQVHPDILAALDRTAATLESMGHVVEQTSAPFGPREWDSFRCVWEVGAAAIP
VLPEREELCTPMSRWLRAGGRRRSGVELAEALAGIQMVARQVGQHWRRFDVIVSPTLAQPPLPVGALRDDEDPAADFDAQ
CRFTPWTSLWNITGCPAMSLPLHNTRIDGVELPIGIMIGGRLYDEATLLALGCALENTRDCERSCR
>Mature_466_residues
MDPLTALETSTRIAAGDVGILDIVDAALEAAHADECHAWTWIDDTGARNTARKLQRQLDAGQARPGPLFGVPCPVKDLNA
LAGAPRAVGSRAVGGTPSDHDDGIVTRLKAAGAIPIGTTAAPEFGLPCYTEPDGRAATVTPWDHTRMAGGSSGGAAAAVG
AGQVPIAQGSDGGGSIRIPASCCGVVGLKPSRGRVSAGPYGIDGPGLVCGGVLTRTVRDTAAALDVLSGPWPGDPFYAPT
PTETFLSACGRDPGRLRIGILREPVIADAQVHPDILAALDRTAATLESMGHVVEQTSAPFGPREWDSFRCVWEVGAAAIP
VLPEREELCTPMSRWLRAGGRRRSGVELAEALAGIQMVARQVGQHWRRFDVIVSPTLAQPPLPVGALRDDEDPAADFDAQ
CRFTPWTSLWNITGCPAMSLPLHNTRIDGVELPIGIMIGGRLYDEATLLALGCALENTRDCERSCR

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=475, Percent_Identity=25.2631578947368, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI195972892, Length=168, Percent_Identity=35.7142857142857, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI166795287, Length=413, Percent_Identity=28.0871670702179, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=244, Percent_Identity=31.9672131147541, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=267, Percent_Identity=30.7116104868914, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=330, Percent_Identity=26.3636363636364, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=184, Percent_Identity=32.0652173913043, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=246, Percent_Identity=31.7073170731707, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=246, Percent_Identity=31.7073170731707, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=135, Percent_Identity=37.7777777777778, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=429, Percent_Identity=26.3403263403263, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24644968, Length=478, Percent_Identity=25.1046025104602, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=193, Percent_Identity=35.7512953367876, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI161078093, Length=291, Percent_Identity=29.2096219931272, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648113, Length=447, Percent_Identity=28.1879194630872, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550774, Length=178, Percent_Identity=30.8988764044944, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24648435, Length=178, Percent_Identity=30.8988764044944, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648437, Length=178, Percent_Identity=30.8988764044944, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24652985, Length=171, Percent_Identity=29.2397660818713, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922090, Length=171, Percent_Identity=29.2397660818713, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652981, Length=171, Percent_Identity=29.2397660818713, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652983, Length=171, Percent_Identity=29.2397660818713, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 48781; Mature: 48781

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPLTALETSTRIAAGDVGILDIVDAALEAAHADECHAWTWIDDTGARNTARKLQRQLDA
CCCCHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHC
GQARPGPLFGVPCPVKDLNALAGAPRAVGSRAVGGTPSDHDDGIVTRLKAAGAIPIGTTA
CCCCCCCEECCCCCHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
APEFGLPCYTEPDGRAATVTPWDHTRMAGGSSGGAAAAVGAGQVPIAQGSDGGGSIRIPA
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECH
SCCGVVGLKPSRGRVSAGPYGIDGPGLVCGGVLTRTVRDTAAALDVLSGPWPGDPFYAPT
HHCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PTETFLSACGRDPGRLRIGILREPVIADAQVHPDILAALDRTAATLESMGHVVEQTSAPF
CHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GPREWDSFRCVWEVGAAAIPVLPEREELCTPMSRWLRAGGRRRSGVELAEALAGIQMVAR
CCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
QVGQHWRRFDVIVSPTLAQPPLPVGALRDDEDPAADFDAQCRFTPWTSLWNITGCPAMSL
HHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHCCCCCCCCEECC
PLHNTRIDGVELPIGIMIGGRLYDEATLLALGCALENTRDCERSCR
CCCCCCCCCEECCEEEEECCEECCCHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MDPLTALETSTRIAAGDVGILDIVDAALEAAHADECHAWTWIDDTGARNTARKLQRQLDA
CCCCHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHC
GQARPGPLFGVPCPVKDLNALAGAPRAVGSRAVGGTPSDHDDGIVTRLKAAGAIPIGTTA
CCCCCCCEECCCCCHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
APEFGLPCYTEPDGRAATVTPWDHTRMAGGSSGGAAAAVGAGQVPIAQGSDGGGSIRIPA
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECH
SCCGVVGLKPSRGRVSAGPYGIDGPGLVCGGVLTRTVRDTAAALDVLSGPWPGDPFYAPT
HHCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PTETFLSACGRDPGRLRIGILREPVIADAQVHPDILAALDRTAATLESMGHVVEQTSAPF
CHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GPREWDSFRCVWEVGAAAIPVLPEREELCTPMSRWLRAGGRRRSGVELAEALAGIQMVAR
CCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
QVGQHWRRFDVIVSPTLAQPPLPVGALRDDEDPAADFDAQCRFTPWTSLWNITGCPAMSL
HHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHCCCCCCCCEECC
PLHNTRIDGVELPIGIMIGGRLYDEATLLALGCALENTRDCERSCR
CCCCCCCCCEECCEEEEECCEECCCHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]