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The map label for this gene is grpE

Identifier: 50843483

GI number: 50843483

Start: 2212934

End: 2213599

Strand: Reverse

Name: grpE

Synonym: PPA2039

Alternate gene names: 50843483

Gene position: 2213599-2212934 (Counterclockwise)

Preceding gene: 50843484

Following gene: 50843482

Centisome position: 86.46

GC content: 61.86

Gene sequence:

>666_bases
ATGACTGATAAGAACGTCAATCCCGACGACGACCAGGCCGACGAGAACCGCGACGACAACGTCGACGAGCGTTTTGCCGA
CATTGTTGGTGACGTCGACATCGACGCTGATCAGTCGGCCACTGACTCGCCGGCTCCGGAGGAACTGTCCCGTGAGTCCC
AACTCGAGGCTCTGCTGGCCGAGCGTACCGAGGACCTGCAGCGTCTACAGGCCGAATACGTCAACTACAAGAGGCGTGTT
GACCGTGACCGCGCGCTGTCGCGTCAGTCCGGTGTGGACAAGGTCATCACCGACCTTATGCCGGTGCTCGACTCAATCGC
CATGGCTCGTCAGCACGGTGAGGTTGAGGGCGGTTTCAAGCTCGTCGTCGACGAGCTGGAGAAGGTCGCCAATAATCACG
GCCTGACCAGTTTCGGCGAGGTGGGTGACGTCTTTGACCCGAACCTCCACGAGGCACTCATGCAGATGCCGATGGAGGGG
GTCTCCGTCACCTCCGTCAGCCAGGTAATGCAGCCCGGCTACAAGCTTGGCGATCGCGTCCTGCGTCCGGCACGTGTGGC
CGTCTCGGACCCGGATCCCAACGCGACCTCAGCCGATGAATCCAGCCAGGCTGATGGCGAGTCCGCTCAGGCTGACGAGG
ATGACGAGACGCCGCGTAACGAGTGA

Upstream 100 bases:

>100_bases
GGACGAGAAAAAGTGACCGGGGCCACGGCCTGACCCCAGCCCCGCCGCCGGGTCGCCATCGCGATGGCCCGGCGGCCGGC
ACTCACGTGAAGGAACACAG

Downstream 100 bases:

>100_bases
CGGCGATCTTGGCCGACTACGATGAGGCCACCATGACCGCAAATCCGACGATGGCCAGGGAGGGAGGAATTAGATGAGTA
CCAAGGACTGGGCTGAGAAG

Product: molecular chaperone GrpE (heat shock protein)

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTDKNVNPDDDQADENRDDNVDERFADIVGDVDIDADQSATDSPAPEELSRESQLEALLAERTEDLQRLQAEYVNYKRRV
DRDRALSRQSGVDKVITDLMPVLDSIAMARQHGEVEGGFKLVVDELEKVANNHGLTSFGEVGDVFDPNLHEALMQMPMEG
VSVTSVSQVMQPGYKLGDRVLRPARVAVSDPDPNATSADESSQADGESAQADEDDETPRNE

Sequences:

>Translated_221_residues
MTDKNVNPDDDQADENRDDNVDERFADIVGDVDIDADQSATDSPAPEELSRESQLEALLAERTEDLQRLQAEYVNYKRRV
DRDRALSRQSGVDKVITDLMPVLDSIAMARQHGEVEGGFKLVVDELEKVANNHGLTSFGEVGDVFDPNLHEALMQMPMEG
VSVTSVSQVMQPGYKLGDRVLRPARVAVSDPDPNATSADESSQADGESAQADEDDETPRNE
>Mature_220_residues
TDKNVNPDDDQADENRDDNVDERFADIVGDVDIDADQSATDSPAPEELSRESQLEALLAERTEDLQRLQAEYVNYKRRVD
RDRALSRQSGVDKVITDLMPVLDSIAMARQHGEVEGGFKLVVDELEKVANNHGLTSFGEVGDVFDPNLHEALMQMPMEGV
SVTSVSQVMQPGYKLGDRVLRPARVAVSDPDPNATSADESSQADGESAQADEDDETPRNE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=171, Percent_Identity=30.9941520467836, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788967, Length=150, Percent_Identity=31.3333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324806, Length=152, Percent_Identity=28.2894736842105, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24653432, Length=167, Percent_Identity=30.5389221556886, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PROAC (Q6A661)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056710.1
- ProteinModelPortal:   Q6A661
- SMR:   Q6A661
- GeneID:   2933033
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA2039
- NMPDR:   fig|267747.1.peg.1987
- HOGENOM:   HBG732630
- OMA:   NETERME
- ProtClustDB:   CLSK2764330
- BioCyc:   PACN267747:PPA2039-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24317; Mature: 24185

Theoretical pI: Translated: 3.92; Mature: 3.92

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKNVNPDDDQADENRDDNVDERFADIVGDVDIDADQSATDSPAPEELSRESQLEALLA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ERTEDLQRLQAEYVNYKRRVDRDRALSRQSGVDKVITDLMPVLDSIAMARQHGEVEGGFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
LVVDELEKVANNHGLTSFGEVGDVFDPNLHEALMQMPMEGVSVTSVSQVMQPGYKLGDRV
HHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
LRPARVAVSDPDPNATSADESSQADGESAQADEDDETPRNE
HCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDKNVNPDDDQADENRDDNVDERFADIVGDVDIDADQSATDSPAPEELSRESQLEALLA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ERTEDLQRLQAEYVNYKRRVDRDRALSRQSGVDKVITDLMPVLDSIAMARQHGEVEGGFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
LVVDELEKVANNHGLTSFGEVGDVFDPNLHEALMQMPMEGVSVTSVSQVMQPGYKLGDRV
HHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
LRPARVAVSDPDPNATSADESSQADGESAQADEDDETPRNE
HCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA