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The map label for this gene is purF [H]

Identifier: 50843417

GI number: 50843417

Start: 2132967

End: 2134454

Strand: Reverse

Name: purF [H]

Synonym: PPA1970

Alternate gene names: 50843417

Gene position: 2134454-2132967 (Counterclockwise)

Preceding gene: 50843418

Following gene: 50843416

Centisome position: 83.37

GC content: 62.3

Gene sequence:

>1488_bases
GTGGCGCGCGCAGACGGAAGGCTTACCGCAGACCTCAACCCCCAGGACCACGGGCCCCAAGACGCCTGTGGCGTCATCGG
GATCTATGCCCCCGGTGAGGAGGTTTCTAAACTCACCTACTTCGGCATGTATGCCCTGCAACACCGTGGCCAGGAATCAG
CTGGCATGGCGGTGTCAGACGGTCGCCACATGATGGTCTTTAAAGACATGGGGTTGGTGTCCCAGGTCTTTGACGAGGCC
ACCCTCAATTCCCTCCAGGGACATATGGCCGTCGGGCACACCCGGTATTCGACGACTGGTGCGAGTATCTGGGACAACGC
CCAGCCGACGTTCAGGTCGCGTCAAGGTGGTGACGGGCTAGCTCTGGCCCATAACGGCAACCTCACCAACACCGGCGCCC
TCGAAGCCCTCATCGCTGAGCGTGCCCCGGATACCGAGGTTCCCCACAAAGACCGGATGGACTCCAGCAACGACACCTCG
CTGGTAACAGCCCTCATGACGACCTATGACGGCACTCTTGAGGAAGTCGCCGCCCAGGTCCTGCCCCACCTTCAGGGTGC
CTTCAGTCTCGTTTTCATGGACGACCACACTTTGTGCGCTGCTCGTGATCCGCAGGGCATCCGACCGTTGGTGCTGGGGC
GCCTATCTAGTGGTTGGGTGGTCGCCTCCGAAACTGCGGCTATCGACATCGTCGGTGGTACTTTCGTTCGCGAGATCGAG
CCCGGCGAGATGGTCGCCATTGACGCGGCTGGGTTGCGCACCAGCCGTTTCGCTGCGGCCCGGCCCAAGGGGTGCGTCTT
CGAATACGTTTACCTGGCTCGCCCTGACACCGTTATTGCTGGACGACGTATCCACAACGTTCGCGTCAAAGTCGGCAAGA
TCCTGGCCCAGGAACACCCTGCCGACGCCGACCTCGTCATCCCGGTGCCAGAGTCTGGCACCCCGGCTGCTATCGGCTAT
GCCGACGAGTCCGGCATCCCCTACGGCATGGGCCTGGTCAAGAACTCCTACGTGGGCCGGACTTTCATCCAGCCCTCTCA
AACCCTGCGCAACTTGGGTATTCGGCTCAAGCTCAACCCGTTGCGTGACGTCATTGAGGGACGACGCATCGTCGTCGTCG
ATGACTCCATCGTGCGCGGCAATACCCAGCGTCAGCTGGTCCGAATGTTGCGTGAGGCTGGTGCGGCCGAGGTGCACGTG
CGCATCTCTTCTCCGCCGGTGCAGTGGCCGTGCTTCTACGGCATTGACTTCGCTACTCGCGCTCAGCTCATCGCCCCGGG
TCTTGACGTCGAGGACATCTGCCGATCTATCGGCGCTGACTCGCTTGGCTACGTCAGCCTTGACGGCCTCGTCCGTGCCA
CCCACGTTGACGCCGATAACCTCTGCCGGGCTTGTTTTGACGGCGTTTATCCCGTACCGGTGCCTGTCTCGGCGCGAGCC
GTGCTAGCCGACGCCACCCACACCAACCCGAACCAGGAGCAGCAGTGA

Upstream 100 bases:

>100_bases
CACTGAACTCGCTTGTCGCCGTCGAGAACGGGTGTGGTGACGCCGACGCTCCGACGCACAATTCACGGTGAGTTGCCTCG
GTGTGCGATAGCGTGTTGAT

Downstream 100 bases:

>100_bases
GCAACGAGTCCGCCTACGCCCGTGCCGGAGTCGACATTGAGGCTGGTGAGCGCGCCGTTGACCTGATGAAGGCCCACGTG
GCCGCCACCCGTCGTCCAGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MARADGRLTADLNPQDHGPQDACGVIGIYAPGEEVSKLTYFGMYALQHRGQESAGMAVSDGRHMMVFKDMGLVSQVFDEA
TLNSLQGHMAVGHTRYSTTGASIWDNAQPTFRSRQGGDGLALAHNGNLTNTGALEALIAERAPDTEVPHKDRMDSSNDTS
LVTALMTTYDGTLEEVAAQVLPHLQGAFSLVFMDDHTLCAARDPQGIRPLVLGRLSSGWVVASETAAIDIVGGTFVREIE
PGEMVAIDAAGLRTSRFAAARPKGCVFEYVYLARPDTVIAGRRIHNVRVKVGKILAQEHPADADLVIPVPESGTPAAIGY
ADESGIPYGMGLVKNSYVGRTFIQPSQTLRNLGIRLKLNPLRDVIEGRRIVVVDDSIVRGNTQRQLVRMLREAGAAEVHV
RISSPPVQWPCFYGIDFATRAQLIAPGLDVEDICRSIGADSLGYVSLDGLVRATHVDADNLCRACFDGVYPVPVPVSARA
VLADATHTNPNQEQQ

Sequences:

>Translated_495_residues
MARADGRLTADLNPQDHGPQDACGVIGIYAPGEEVSKLTYFGMYALQHRGQESAGMAVSDGRHMMVFKDMGLVSQVFDEA
TLNSLQGHMAVGHTRYSTTGASIWDNAQPTFRSRQGGDGLALAHNGNLTNTGALEALIAERAPDTEVPHKDRMDSSNDTS
LVTALMTTYDGTLEEVAAQVLPHLQGAFSLVFMDDHTLCAARDPQGIRPLVLGRLSSGWVVASETAAIDIVGGTFVREIE
PGEMVAIDAAGLRTSRFAAARPKGCVFEYVYLARPDTVIAGRRIHNVRVKVGKILAQEHPADADLVIPVPESGTPAAIGY
ADESGIPYGMGLVKNSYVGRTFIQPSQTLRNLGIRLKLNPLRDVIEGRRIVVVDDSIVRGNTQRQLVRMLREAGAAEVHV
RISSPPVQWPCFYGIDFATRAQLIAPGLDVEDICRSIGADSLGYVSLDGLVRATHVDADNLCRACFDGVYPVPVPVSARA
VLADATHTNPNQEQQ
>Mature_494_residues
ARADGRLTADLNPQDHGPQDACGVIGIYAPGEEVSKLTYFGMYALQHRGQESAGMAVSDGRHMMVFKDMGLVSQVFDEAT
LNSLQGHMAVGHTRYSTTGASIWDNAQPTFRSRQGGDGLALAHNGNLTNTGALEALIAERAPDTEVPHKDRMDSSNDTSL
VTALMTTYDGTLEEVAAQVLPHLQGAFSLVFMDDHTLCAARDPQGIRPLVLGRLSSGWVVASETAAIDIVGGTFVREIEP
GEMVAIDAAGLRTSRFAAARPKGCVFEYVYLARPDTVIAGRRIHNVRVKVGKILAQEHPADADLVIPVPESGTPAAIGYA
DESGIPYGMGLVKNSYVGRTFIQPSQTLRNLGIRLKLNPLRDVIEGRRIVVVDDSIVRGNTQRQLVRMLREAGAAEVHVR
ISSPPVQWPCFYGIDFATRAQLIAPGLDVEDICRSIGADSLGYVSLDGLVRATHVDADNLCRACFDGVYPVPVPVSARAV
LADATHTNPNQEQQ

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=38.150289017341, Blast_Score=338, Evalue=7e-93,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=37.6050420168067, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790167, Length=232, Percent_Identity=29.3103448275862, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=488, Percent_Identity=38.3196721311475, Blast_Score=297, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6323958, Length=467, Percent_Identity=33.8329764453961, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24659598, Length=489, Percent_Identity=41.3087934560327, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=42.274678111588, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI28573187, Length=502, Percent_Identity=40.0398406374502, Blast_Score=349, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53245; Mature: 53114

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARADGRLTADLNPQDHGPQDACGVIGIYAPGEEVSKLTYFGMYALQHRGQESAGMAVSD
CCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEECC
GRHMMVFKDMGLVSQVFDEATLNSLQGHMAVGHTRYSTTGASIWDNAQPTFRSRQGGDGL
CCEEEEEECCHHHHHHHHHHHHHHHCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCEE
ALAHNGNLTNTGALEALIAERAPDTEVPHKDRMDSSNDTSLVTALMTTYDGTLEEVAAQV
EEEECCCCCCCHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
LPHLQGAFSLVFMDDHTLCAARDPQGIRPLVLGRLSSGWVVASETAAIDIVGGTFVREIE
HHHCCCCEEEEEECCCEEEECCCCCCCCHHHEEECCCCEEEEECCEEEEEECCEEEEECC
PGEMVAIDAAGLRTSRFAAARPKGCVFEYVYLARPDTVIAGRRIHNVRVKVGKILAQEHP
CCCEEEEECCCCCCCHHHCCCCCCCEEEEEEEECCCEEEECCEEEEEEEHHHHHHHHCCC
ADADLVIPVPESGTPAAIGYADESGIPYGMGLVKNSYVGRTFIQPSQTLRNLGIRLKLNP
CCCEEEEECCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCEECCHHHHHHHCCCEEEECH
LRDVIEGRRIVVVDDSIVRGNTQRQLVRMLREAGAAEVHVRISSPPVQWPCFYGIDFATR
HHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCHHHH
AQLIAPGLDVEDICRSIGADSLGYVSLDGLVRATHVDADNLCRACFDGVYPVPVPVSARA
HHHCCCCCCHHHHHHHHCCCCCCEEEECCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCE
VLADATHTNPNQEQQ
EEEECCCCCCCCCCC
>Mature Secondary Structure 
ARADGRLTADLNPQDHGPQDACGVIGIYAPGEEVSKLTYFGMYALQHRGQESAGMAVSD
CCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEECC
GRHMMVFKDMGLVSQVFDEATLNSLQGHMAVGHTRYSTTGASIWDNAQPTFRSRQGGDGL
CCEEEEEECCHHHHHHHHHHHHHHHCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCEE
ALAHNGNLTNTGALEALIAERAPDTEVPHKDRMDSSNDTSLVTALMTTYDGTLEEVAAQV
EEEECCCCCCCHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
LPHLQGAFSLVFMDDHTLCAARDPQGIRPLVLGRLSSGWVVASETAAIDIVGGTFVREIE
HHHCCCCEEEEEECCCEEEECCCCCCCCHHHEEECCCCEEEEECCEEEEEECCEEEEECC
PGEMVAIDAAGLRTSRFAAARPKGCVFEYVYLARPDTVIAGRRIHNVRVKVGKILAQEHP
CCCEEEEECCCCCCCHHHCCCCCCCEEEEEEEECCCEEEECCEEEEEEEHHHHHHHHCCC
ADADLVIPVPESGTPAAIGYADESGIPYGMGLVKNSYVGRTFIQPSQTLRNLGIRLKLNP
CCCEEEEECCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCEECCHHHHHHHCCCEEEECH
LRDVIEGRRIVVVDDSIVRGNTQRQLVRMLREAGAAEVHVRISSPPVQWPCFYGIDFATR
HHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCHHHH
AQLIAPGLDVEDICRSIGADSLGYVSLDGLVRATHVDADNLCRACFDGVYPVPVPVSARA
HHHCCCCCCHHHHHHHHCCCCCCEEEECCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCE
VLADATHTNPNQEQQ
EEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]