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The map label for this gene is nuoI

Identifier: 50843378

GI number: 50843378

Start: 2088173

End: 2088769

Strand: Reverse

Name: nuoI

Synonym: PPA1928

Alternate gene names: 50843378

Gene position: 2088769-2088173 (Counterclockwise)

Preceding gene: 50843379

Following gene: 50843377

Centisome position: 81.58

GC content: 60.64

Gene sequence:

>597_bases
ATGGGAGCCTGGTCCGGATTCGGGATCACCTTCGAGACGATGTTCCGCAAGTCCTTCACCCAGGGATACCCGGAGAAGGG
CAAGGAAAAGCCGATGCCGCCGCGCATGCATGGCCGTCACCAGCTCAACCGCTGGCCTGACGGGTTGGAAAAGTGTGTGG
GTTGTGAGCTGTGTGCATGGGCGTGCCCGGCCGATGCCATCTACGTCGAGGGGGCAGATAACACTGACGAGGAGCGTTAC
TCGCCCGGTGAGCGTTACGGTCGCGTGTACGAGATCAACTATCTGCGCTGCATTTTGTGCGGAATGTGCATCGAGGCATG
CCCAACTCGTGCCCTGACGATGACGAACGATTTCAAGATGGCCGACACCTCGCGTGCCAAGCACATTTGGACGAAGGACG
AGTTGCTGGCTCCCCTCAAGGAGGGCATGGAGCAGCCGCCACACCCGCGCCGTCTCGGTGATGACGAAGATGACTACTTC
AACGGTCTTCCTCCCTCTGGGCAGGACGACGTACGGACCGGTCCGGCCAATAGTGGTGCCACTGCGCACCGGGACGATGA
CAACGACACCCAACACAAGGATGAGGAGGCCGCCTGA

Upstream 100 bases:

>100_bases
CGGTACCGCCATTGCCACATCAGGTGCAGGCCCCATTGGCTGGGGCTGCAACGGCCACCACGGTGGCTCGACGAGACCAC
GACGAGAACGGAGGTCTGTG

Downstream 100 bases:

>100_bases
TGACTACGGGACTCCCCCTGCTCGCCGGTCTCGTACCTATGGTGACGGCATCCCAGTGGGCCTTCTGGCTGTTGGCACCG
ATCATGGTGCTTGCGGCCTT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MGAWSGFGITFETMFRKSFTQGYPEKGKEKPMPPRMHGRHQLNRWPDGLEKCVGCELCAWACPADAIYVEGADNTDEERY
SPGERYGRVYEINYLRCILCGMCIEACPTRALTMTNDFKMADTSRAKHIWTKDELLAPLKEGMEQPPHPRRLGDDEDDYF
NGLPPSGQDDVRTGPANSGATAHRDDDNDTQHKDEEAA

Sequences:

>Translated_198_residues
MGAWSGFGITFETMFRKSFTQGYPEKGKEKPMPPRMHGRHQLNRWPDGLEKCVGCELCAWACPADAIYVEGADNTDEERY
SPGERYGRVYEINYLRCILCGMCIEACPTRALTMTNDFKMADTSRAKHIWTKDELLAPLKEGMEQPPHPRRLGDDEDDYF
NGLPPSGQDDVRTGPANSGATAHRDDDNDTQHKDEEAA
>Mature_197_residues
GAWSGFGITFETMFRKSFTQGYPEKGKEKPMPPRMHGRHQLNRWPDGLEKCVGCELCAWACPADAIYVEGADNTDEERYS
PGERYGRVYEINYLRCILCGMCIEACPTRALTMTNDFKMADTSRAKHIWTKDELLAPLKEGMEQPPHPRRLGDDEDDYFN
GLPPSGQDDVRTGPANSGATAHRDDDNDTQHKDEEAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=134, Percent_Identity=37.3134328358209, Blast_Score=98, Evalue=4e-21,
Organism=Escherichia coli, GI1788617, Length=138, Percent_Identity=40.5797101449275, Blast_Score=101, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17555194, Length=134, Percent_Identity=35.8208955223881, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17864306, Length=132, Percent_Identity=37.1212121212121, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_PROAC (Q6A6G6)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056605.1
- ProteinModelPortal:   Q6A6G6
- SMR:   Q6A6G6
- GeneID:   2931976
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1928
- NMPDR:   fig|267747.1.peg.1882
- HOGENOM:   HBG724359
- OMA:   VEGGDNT
- ProtClustDB:   PRK05888
- BioCyc:   PACN267747:PPA1928-MONOMER
- BRENDA:   1.6.99.5
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 22337; Mature: 22206

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAWSGFGITFETMFRKSFTQGYPEKGKEKPMPPRMHGRHQLNRWPDGLEKCVGCELCAW
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCHHHHH
ACPADAIYVEGADNTDEERYSPGERYGRVYEINYLRCILCGMCIEACPTRALTMTNDFKM
CCCCCEEEEECCCCCCHHHCCCHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCC
ADTSRAKHIWTKDELLAPLKEGMEQPPHPRRLGDDEDDYFNGLPPSGQDDVRTGPANSGA
CCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
TAHRDDDNDTQHKDEEAA
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GAWSGFGITFETMFRKSFTQGYPEKGKEKPMPPRMHGRHQLNRWPDGLEKCVGCELCAW
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCHHHHH
ACPADAIYVEGADNTDEERYSPGERYGRVYEINYLRCILCGMCIEACPTRALTMTNDFKM
CCCCCEEEEECCCCCCHHHCCCHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCC
ADTSRAKHIWTKDELLAPLKEGMEQPPHPRRLGDDEDDYFNGLPPSGQDDVRTGPANSGA
CCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
TAHRDDDNDTQHKDEEAA
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA