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The map label for this gene is trmB [H]

Identifier: 50843265

GI number: 50843265

Start: 1972797

End: 1973741

Strand: Direct

Name: trmB [H]

Synonym: PPA1808

Alternate gene names: 50843265

Gene position: 1972797-1973741 (Clockwise)

Preceding gene: 50843263

Following gene: 50843266

Centisome position: 77.05

GC content: 65.4

Gene sequence:

>945_bases
ATGTCAAAATATTCATGCTCGCCAACTTTTGACGTAGATCGCCGCTCCATCACCTCAGCCGGCGAACACTTCAATCACCG
CGTCGCTCAGAGTCATCGACTATCATTCGACCCCGTGCGACCCGATCCCGCTCCCCTCGATCCGACCGACGCCTCCCCCG
CCCAGGCCCGCCGCCACCAGCCGGGGCTAGGGGTCGAGGTGTCAGCCGGGGCACACCGCTCGGGCGACCGGGTCCGGCGC
GGTGTCGTCTCCTTCGTGCGGCGATCCCCCCGGATGAACGTCTCTCAGCAACGGGCCATGAATACCTTGGCCAGCACGTA
CCTCATCGACGTCCCCCGCGACGCCACCAGCACCTCGGTCGCCCCTGGATCCCGTCTAGACCTCCCCGCGATCTTCGGTC
GCACCGCCCCGCTTACCGTAGAGATCGGAGTTGGGTCCGGCGACGTGCTGGCAGCGCTAGCGGCCGCCCATCCCGAACGA
GACTTCATCGGTTTCGAGGTCTACCTGCCCTCCATCGCGACGACCCTCAACAAGCTCGAGAACGCTGGTGCAAGTAACGC
CCGGGTCATCATGGCCGACGCCACAGCAGGGCTGGACCACCTCTTCGGACCCGCCGACCTCGACGAATTGTGGACCTTCT
TCGCTGATCCATGGCACAAGAAGCGTCACCACAAACGTCGCATCGTCAACCCCGACACAGCCCGTTTGGTGACGTCCCGG
CTGCGTCCGGGCGGTCTGTGGCGTCTGGCCACCGACTGGGACGACTACGCCCACTGGATGCTCGAAGTACTCTCAGCCGA
GCCGCTGCTCAAGCCGGTCGACGCCGGGCCGGACGGTTTTAGCCCGCGCTGGCCCGAACGGCCAGTGACCCGCTACGAGA
ACAAGGGGCTGACGGCCGGACGCACTATTCACGACCTCACCTGGCGTCGCGTGGACGAGTCATGA

Upstream 100 bases:

>100_bases
ATCTCAGATCGCCTAGCGACAAGGAGTGACGCCTGGAATCAGCGAAGCCCGACCTCTGTTTTCCCAGCCTCTTTCTGGCC
CATTTTTCACAACACTACCA

Downstream 100 bases:

>100_bases
CCCGCTGGCGCCTGGACATCGCCTATGACGGGGCGAACTTCTCGGGGTGGGCCACCCAACCCGATCGCCGCACCGTACAG
GGTGAGCTGGAAACGTGGAT

Product: methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSKYSCSPTFDVDRRSITSAGEHFNHRVAQSHRLSFDPVRPDPAPLDPTDASPAQARRHQPGLGVEVSAGAHRSGDRVRR
GVVSFVRRSPRMNVSQQRAMNTLASTYLIDVPRDATSTSVAPGSRLDLPAIFGRTAPLTVEIGVGSGDVLAALAAAHPER
DFIGFEVYLPSIATTLNKLENAGASNARVIMADATAGLDHLFGPADLDELWTFFADPWHKKRHHKRRIVNPDTARLVTSR
LRPGGLWRLATDWDDYAHWMLEVLSAEPLLKPVDAGPDGFSPRWPERPVTRYENKGLTAGRTIHDLTWRRVDES

Sequences:

>Translated_314_residues
MSKYSCSPTFDVDRRSITSAGEHFNHRVAQSHRLSFDPVRPDPAPLDPTDASPAQARRHQPGLGVEVSAGAHRSGDRVRR
GVVSFVRRSPRMNVSQQRAMNTLASTYLIDVPRDATSTSVAPGSRLDLPAIFGRTAPLTVEIGVGSGDVLAALAAAHPER
DFIGFEVYLPSIATTLNKLENAGASNARVIMADATAGLDHLFGPADLDELWTFFADPWHKKRHHKRRIVNPDTARLVTSR
LRPGGLWRLATDWDDYAHWMLEVLSAEPLLKPVDAGPDGFSPRWPERPVTRYENKGLTAGRTIHDLTWRRVDES
>Mature_313_residues
SKYSCSPTFDVDRRSITSAGEHFNHRVAQSHRLSFDPVRPDPAPLDPTDASPAQARRHQPGLGVEVSAGAHRSGDRVRRG
VVSFVRRSPRMNVSQQRAMNTLASTYLIDVPRDATSTSVAPGSRLDLPAIFGRTAPLTVEIGVGSGDVLAALAAAHPERD
FIGFEVYLPSIATTLNKLENAGASNARVIMADATAGLDHLFGPADLDELWTFFADPWHKKRHHKRRIVNPDTARLVTSRL
RPGGLWRLATDWDDYAHWMLEVLSAEPLLKPVDAGPDGFSPRWPERPVTRYENKGLTAGRTIHDLTWRRVDES

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=232, Percent_Identity=40.0862068965517, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 34701; Mature: 34570

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYSCSPTFDVDRRSITSAGEHFNHRVAQSHRLSFDPVRPDPAPLDPTDASPAQARRHQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCC
PGLGVEVSAGAHRSGDRVRRGVVSFVRRSPRMNVSQQRAMNTLASTYLIDVPRDATSTSV
CCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCC
APGSRLDLPAIFGRTAPLTVEIGVGSGDVLAALAAAHPERDFIGFEVYLPSIATTLNKLE
CCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHH
NAGASNARVIMADATAGLDHLFGPADLDELWTFFADPWHKKRHHKRRIVNPDTARLVTSR
HCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHH
LRPGGLWRLATDWDDYAHWMLEVLSAEPLLKPVDAGPDGFSPRWPERPVTRYENKGLTAG
CCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
RTIHDLTWRRVDES
CHHHHHHHHHCCCC
>Mature Secondary Structure 
SKYSCSPTFDVDRRSITSAGEHFNHRVAQSHRLSFDPVRPDPAPLDPTDASPAQARRHQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCC
PGLGVEVSAGAHRSGDRVRRGVVSFVRRSPRMNVSQQRAMNTLASTYLIDVPRDATSTSV
CCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCC
APGSRLDLPAIFGRTAPLTVEIGVGSGDVLAALAAAHPERDFIGFEVYLPSIATTLNKLE
CCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHH
NAGASNARVIMADATAGLDHLFGPADLDELWTFFADPWHKKRHHKRRIVNPDTARLVTSR
HCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHH
LRPGGLWRLATDWDDYAHWMLEVLSAEPLLKPVDAGPDGFSPRWPERPVTRYENKGLTAG
CCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
RTIHDLTWRRVDES
CHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA