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The map label for this gene is truA

Identifier: 50843266

GI number: 50843266

Start: 1973738

End: 1974577

Strand: Direct

Name: truA

Synonym: PPA1809

Alternate gene names: 50843266

Gene position: 1973738-1974577 (Clockwise)

Preceding gene: 50843265

Following gene: 50843267

Centisome position: 77.09

GC content: 66.9

Gene sequence:

>840_bases
ATGACCCGCTGGCGCCTGGACATCGCCTATGACGGGGCGAACTTCTCGGGGTGGGCCACCCAACCCGATCGCCGCACCGT
ACAGGGTGAGCTGGAAACGTGGATTCCCCGCGTACTCCGTCTCGACCAGCCCACTCCCCTAACCGTGGCGGGGCGTACTG
ATGCTGGCGTTCATGCCCGCGGCCAAGTGGCCCACGTCGACCTGCCCGACAACGTCGACACCTCAGCGATGCTGCGTCGG
CTGTCCCGGGTCCTCACCCCCGACGTCGTCGTAAAGTCGGTGCGCCCCGTCCCCAGCACGTTCGACGCCCGGTTCTCGGC
ACTATGGCGTCGCTACGTTTACCGCCTGTGGGACGAGTCCTCCCGTCCTGACCCGGTGACCCGGTTCCACGTGGCACCCG
TGCGGGGACACCTCGACCTCGACCGCCTCAACACCGCCGGCACTTCCCTTCTCGGGCTGCGCGACTTCGCGGCCTTCTGC
AAACACCGCGAGGGCGCCACAACAATCCGTACCCTCCTCGACTGCCACGCCAAACGGCTCGACGACCCGTGCGGCACCGT
CGAAGTGACCGTCCGCGCTGACGCCTTTTGTCACTCCATGGTGAGATCCCTCGTCGGAGCTCTCACCGCAGTCGCCTCGG
GGCGGCGCAGCCAAGACTGGCTCGACAACGTCGCGGCCAGTACCTCGCGGGCATCATCAGTGCTCGTCATGCCGGCCTGC
GGACTCACCCTCGAAGAGGTCGGCTACCCGTCCGACGAGGATCTCGCTCAACGCGCTGCCCAAGCCCGTTCCCGTCGAAG
CCACAACGACATCTGCGACACCTGTTGGGAGGACCGATGA

Upstream 100 bases:

>100_bases
GCCCGCGCTGGCCCGAACGGCCAGTGACCCGCTACGAGAACAAGGGGCTGACGGCCGGACGCACTATTCACGACCTCACC
TGGCGTCGCGTGGACGAGTC

Downstream 100 bases:

>100_bases
GCCAATCCAGCCATGACGACCATTCCCCCACCCACTACTTCACCACCCCAGAGGGACCCACCCGCACCCGCACTATCGCC
GTCAACGTCTGGGATGATTC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTRWRLDIAYDGANFSGWATQPDRRTVQGELETWIPRVLRLDQPTPLTVAGRTDAGVHARGQVAHVDLPDNVDTSAMLRR
LSRVLTPDVVVKSVRPVPSTFDARFSALWRRYVYRLWDESSRPDPVTRFHVAPVRGHLDLDRLNTAGTSLLGLRDFAAFC
KHREGATTIRTLLDCHAKRLDDPCGTVEVTVRADAFCHSMVRSLVGALTAVASGRRSQDWLDNVAASTSRASSVLVMPAC
GLTLEEVGYPSDEDLAQRAAQARSRRSHNDICDTCWEDR

Sequences:

>Translated_279_residues
MTRWRLDIAYDGANFSGWATQPDRRTVQGELETWIPRVLRLDQPTPLTVAGRTDAGVHARGQVAHVDLPDNVDTSAMLRR
LSRVLTPDVVVKSVRPVPSTFDARFSALWRRYVYRLWDESSRPDPVTRFHVAPVRGHLDLDRLNTAGTSLLGLRDFAAFC
KHREGATTIRTLLDCHAKRLDDPCGTVEVTVRADAFCHSMVRSLVGALTAVASGRRSQDWLDNVAASTSRASSVLVMPAC
GLTLEEVGYPSDEDLAQRAAQARSRRSHNDICDTCWEDR
>Mature_278_residues
TRWRLDIAYDGANFSGWATQPDRRTVQGELETWIPRVLRLDQPTPLTVAGRTDAGVHARGQVAHVDLPDNVDTSAMLRRL
SRVLTPDVVVKSVRPVPSTFDARFSALWRRYVYRLWDESSRPDPVTRFHVAPVRGHLDLDRLNTAGTSLLGLRDFAAFCK
HREGATTIRTLLDCHAKRLDDPCGTVEVTVRADAFCHSMVRSLVGALTAVASGRRSQDWLDNVAASTSRASSVLVMPACG
LTLEEVGYPSDEDLAQRAAQARSRRSHNDICDTCWEDR

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Escherichia coli, GI1788657, Length=256, Percent_Identity=34.375, Blast_Score=132, Evalue=3e-32,
Organism=Drosophila melanogaster, GI116007752, Length=305, Percent_Identity=27.8688524590164, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PROAC (Q6A6S8)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056493.1
- ProteinModelPortal:   Q6A6S8
- SMR:   Q6A6S8
- GeneID:   2932940
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1809
- NMPDR:   fig|267747.1.peg.1770
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   PACN267747:PPA1809-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 31092; Mature: 30961

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRWRLDIAYDGANFSGWATQPDRRTVQGELETWIPRVLRLDQPTPLTVAGRTDAGVHAR
CCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
GQVAHVDLPDNVDTSAMLRRLSRVLTPDVVVKSVRPVPSTFDARFSALWRRYVYRLWDES
CCEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
SRPDPVTRFHVAPVRGHLDLDRLNTAGTSLLGLRDFAAFCKHREGATTIRTLLDCHAKRL
CCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
DDPCGTVEVTVRADAFCHSMVRSLVGALTAVASGRRSQDWLDNVAASTSRASSVLVMPAC
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCC
GLTLEEVGYPSDEDLAQRAAQARSRRSHNDICDTCWEDR
CCCHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure 
TRWRLDIAYDGANFSGWATQPDRRTVQGELETWIPRVLRLDQPTPLTVAGRTDAGVHAR
CEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
GQVAHVDLPDNVDTSAMLRRLSRVLTPDVVVKSVRPVPSTFDARFSALWRRYVYRLWDES
CCEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
SRPDPVTRFHVAPVRGHLDLDRLNTAGTSLLGLRDFAAFCKHREGATTIRTLLDCHAKRL
CCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
DDPCGTVEVTVRADAFCHSMVRSLVGALTAVASGRRSQDWLDNVAASTSRASSVLVMPAC
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCC
GLTLEEVGYPSDEDLAQRAAQARSRRSHNDICDTCWEDR
CCCHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA