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The map label for this gene is pcrA [H]

Identifier: 50843216

GI number: 50843216

Start: 1917807

End: 1920239

Strand: Reverse

Name: pcrA [H]

Synonym: PPA1756

Alternate gene names: 50843216

Gene position: 1920239-1917807 (Counterclockwise)

Preceding gene: 50843217

Following gene: 50843215

Centisome position: 75.0

GC content: 55.73

Gene sequence:

>2433_bases
ATGACGACACCGGATCTGTTTTCGTTTTTCGAATCGGCTCATGAGAGACGCAGCCCTGTTGAGCCAGGGGTTTTGCCGAC
CTATGCCCCCGTGACGGCCGGAGCAGCCTCTCCTGCTGACTTACTGGCCGATCTCAACGAGCCCCAACGCGAGGCGGTGT
TGCACGCCGGTTCGCCGGTCCTCGTTGTCGCGGGTGCAGGCTCGGGAAAGACGAGAGTTCTCACGCGACGCATCGCCCAC
CTGGTGGGGGAACGAGGCGTTCATCCTGGGTCGATTTTGGCCATCACCTTCACCAATAAAGCGGCGGCCGAGATGAAGGC
GCGCGTCGTTGACCTGGTGGGAAATCGCGCTAAACCGATGTGGGTGTCGACCTTCCATTCTGCCGCGGTGAGAATACTAC
GCACCGACATTGACCGTCTGGGTATTTCCCGTAATTTCTCAATCTATGACGATACGGACGCTAAAAGACTCATCACTCTC
GTTACCCGTGACCAGAATCTGGATCCTAAGAAGTTCCAGCCTCGCGCTATCATGAACTGGATCTCACATCAAAAAAACGA
GCTCGTTGCCCCGGATCAAGCAGTCGCGGCTGCCAAAAGCGGAAATGATCGAATTAATGGTGACGTCTACGTCGAGTACC
AACGCAGACTACGAGCTGCCAATGTGCTAGATTTCGATGATCTTATTATGTATACCGTCACCGTGTTGAGGAAGTTTCCT
GAGGTTCGCGAACGGTATCGACGTCGGTTTCGGCATGTACTCGTTGATGAGTATCAGGACACTAACACCGCACAATATCA
GTTCATTCGTGAGCTGTGTGGGGATGGCCCAGTCGAGCTAGATGACGGTGCTCGTAGTGTCGATCCGCCGGAGCTCATGG
TGGTTGGCGATTCAGATCAGTCGATTTACGCATTTCGTGGCGCGACAATCCGTAACATCATTGACTTTGAAAAGGATTTT
CCGGGGGCTAGGACGATTCTCCTGGAGCAGAATTATCGCTCGACTCAGACGATTCTTACCGCTGCAAACAATCTCATTAA
GGTCAATCTAAATCGTCCCGCCAAAAACTTGTGGACTGATCAGGGCGATGGTGACAAAATCGTCGGGTATGTTGCCGACA
CTGAACATGACGAGGCTGCCTGGGTGGCCCGACGTATTGACGAACTCGTCGATGAGGGACGTACCAGCTATGGACAAGTA
GCCGTTTTTTACCGTACCAACGCCCAATCGAGGGCTTTCGAGGATGTCTTTATTCGCACTGGCTTGCCCTACCGTGTCGT
TGGCGGTGTGAGGTTCTACGAGCGCAAAGAAGTCCGTGACGCCGTCAGTTACCTGCGTGCGATCGCTAACCCCTCCGATG
ACGTTTCCCTGCGTCGCATTCTCAATACTCCGCGCCGTGGCATCGGAGCGCGAGCCGAAGCAGCAGTCGAGATGTTTGCT
ACTGCTCGCAGAATTAGCTTCTGGGAAGCTTTGAAACGGATCGATGAAATCGATAGTCTAGCAACCAGATCGGTCAATCA
AATTCGAGCTTTCGTCAGCCTCATGGATGGCCATATCGCCATGGTGGAGCAGGGGAAACGGGCCGACGAGATCGCCAGCT
CGATCCTGAAAGAATCCGGTTACATCACCGAACTAGAGAACTCCAAAGACCCGCAGGATGAGACGAGGCTGGAAAACCTC
ATCGAGCTTGTTTCGGTGGCTCAGGAATTCGTTGCCCGAGTCCATGCTGTCGATATTGACGAGGCTGAGCAGGGCAACGT
CGAAGACCTTGAATTGTCCGGCGACGACTCTTTGCCGGCCTTCCTGGAGCAGATCGCCTTGGTCGCCGATTCCGACCAGG
TACCAGCCGACTCCGATGGAGTCGTCACCCTCATGACCCTGCACACCGCCAAAGGTTTGGAGTTCGACACAGTTTTCATC
ACTGGATTTGAGGACGGTGTCTTCCCTCACATGAGGTCTCTTGCCGACGCCACTGAATTGTCTGAGGAGAGACGGCTGGC
TTACGTCGGTATCACTCGCGCTCGCAAACAGCTGTTTGTGTCGCGGGCCTCTGTGCGCACGATGTGGGGCCAGGCTCAGT
ACAACCCACCGAGCCGATTCATCGACGAGATCCCTGAACACCTCATTGAGTGGGAGAGAATCGGCCAGGCCTCGGTGGGA
TGGTCAACTTCGGCGAGTGGCCGCTCGCAATCAGCACGCGGGGCCCTTCGCGACGACACTCCGGCCTTCGGTTCTGGACG
AGGGTCAGCAAGTGCGCTCAAGGGACGCACCCTCGCGCTGTCGGTAGGGGATAAAGTGCTTCACTCGACCTTCGGGCTGG
GGACCGTCAAAGCCACGTCAGGCCAGGGCGACAATGCCAAGGCTGATGTCGACTTCGGGTCAGCGGGGCTCAAGAGGTTG
GCGTTGAGGTTCGCTCCACTGGAGAAGCTGTAA

Upstream 100 bases:

>100_bases
TGAGGATCACTGTCAATACGGGGCCGTTGGTTCGCTGTGTGAACATGCGATGGGTTGTGCCAGCTGGGGACGACTATGGG
CCGGTAGGCTGTGAGTCGCC

Downstream 100 bases:

>100_bases
GTGGATGCTGACGTAGTCTTCGGTCGCTACGAAAGATCAGATTGCTCGACGTCGTGAGATCAGGTGGGCATCGGGCCTCC
GGGGTTGTTAGTCTGTTCAT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MTTPDLFSFFESAHERRSPVEPGVLPTYAPVTAGAASPADLLADLNEPQREAVLHAGSPVLVVAGAGSGKTRVLTRRIAH
LVGERGVHPGSILAITFTNKAAAEMKARVVDLVGNRAKPMWVSTFHSAAVRILRTDIDRLGISRNFSIYDDTDAKRLITL
VTRDQNLDPKKFQPRAIMNWISHQKNELVAPDQAVAAAKSGNDRINGDVYVEYQRRLRAANVLDFDDLIMYTVTVLRKFP
EVRERYRRRFRHVLVDEYQDTNTAQYQFIRELCGDGPVELDDGARSVDPPELMVVGDSDQSIYAFRGATIRNIIDFEKDF
PGARTILLEQNYRSTQTILTAANNLIKVNLNRPAKNLWTDQGDGDKIVGYVADTEHDEAAWVARRIDELVDEGRTSYGQV
AVFYRTNAQSRAFEDVFIRTGLPYRVVGGVRFYERKEVRDAVSYLRAIANPSDDVSLRRILNTPRRGIGARAEAAVEMFA
TARRISFWEALKRIDEIDSLATRSVNQIRAFVSLMDGHIAMVEQGKRADEIASSILKESGYITELENSKDPQDETRLENL
IELVSVAQEFVARVHAVDIDEAEQGNVEDLELSGDDSLPAFLEQIALVADSDQVPADSDGVVTLMTLHTAKGLEFDTVFI
TGFEDGVFPHMRSLADATELSEERRLAYVGITRARKQLFVSRASVRTMWGQAQYNPPSRFIDEIPEHLIEWERIGQASVG
WSTSASGRSQSARGALRDDTPAFGSGRGSASALKGRTLALSVGDKVLHSTFGLGTVKATSGQGDNAKADVDFGSAGLKRL
ALRFAPLEKL

Sequences:

>Translated_810_residues
MTTPDLFSFFESAHERRSPVEPGVLPTYAPVTAGAASPADLLADLNEPQREAVLHAGSPVLVVAGAGSGKTRVLTRRIAH
LVGERGVHPGSILAITFTNKAAAEMKARVVDLVGNRAKPMWVSTFHSAAVRILRTDIDRLGISRNFSIYDDTDAKRLITL
VTRDQNLDPKKFQPRAIMNWISHQKNELVAPDQAVAAAKSGNDRINGDVYVEYQRRLRAANVLDFDDLIMYTVTVLRKFP
EVRERYRRRFRHVLVDEYQDTNTAQYQFIRELCGDGPVELDDGARSVDPPELMVVGDSDQSIYAFRGATIRNIIDFEKDF
PGARTILLEQNYRSTQTILTAANNLIKVNLNRPAKNLWTDQGDGDKIVGYVADTEHDEAAWVARRIDELVDEGRTSYGQV
AVFYRTNAQSRAFEDVFIRTGLPYRVVGGVRFYERKEVRDAVSYLRAIANPSDDVSLRRILNTPRRGIGARAEAAVEMFA
TARRISFWEALKRIDEIDSLATRSVNQIRAFVSLMDGHIAMVEQGKRADEIASSILKESGYITELENSKDPQDETRLENL
IELVSVAQEFVARVHAVDIDEAEQGNVEDLELSGDDSLPAFLEQIALVADSDQVPADSDGVVTLMTLHTAKGLEFDTVFI
TGFEDGVFPHMRSLADATELSEERRLAYVGITRARKQLFVSRASVRTMWGQAQYNPPSRFIDEIPEHLIEWERIGQASVG
WSTSASGRSQSARGALRDDTPAFGSGRGSASALKGRTLALSVGDKVLHSTFGLGTVKATSGQGDNAKADVDFGSAGLKRL
ALRFAPLEKL
>Mature_809_residues
TTPDLFSFFESAHERRSPVEPGVLPTYAPVTAGAASPADLLADLNEPQREAVLHAGSPVLVVAGAGSGKTRVLTRRIAHL
VGERGVHPGSILAITFTNKAAAEMKARVVDLVGNRAKPMWVSTFHSAAVRILRTDIDRLGISRNFSIYDDTDAKRLITLV
TRDQNLDPKKFQPRAIMNWISHQKNELVAPDQAVAAAKSGNDRINGDVYVEYQRRLRAANVLDFDDLIMYTVTVLRKFPE
VRERYRRRFRHVLVDEYQDTNTAQYQFIRELCGDGPVELDDGARSVDPPELMVVGDSDQSIYAFRGATIRNIIDFEKDFP
GARTILLEQNYRSTQTILTAANNLIKVNLNRPAKNLWTDQGDGDKIVGYVADTEHDEAAWVARRIDELVDEGRTSYGQVA
VFYRTNAQSRAFEDVFIRTGLPYRVVGGVRFYERKEVRDAVSYLRAIANPSDDVSLRRILNTPRRGIGARAEAAVEMFAT
ARRISFWEALKRIDEIDSLATRSVNQIRAFVSLMDGHIAMVEQGKRADEIASSILKESGYITELENSKDPQDETRLENLI
ELVSVAQEFVARVHAVDIDEAEQGNVEDLELSGDDSLPAFLEQIALVADSDQVPADSDGVVTLMTLHTAKGLEFDTVFIT
GFEDGVFPHMRSLADATELSEERRLAYVGITRARKQLFVSRASVRTMWGQAQYNPPSRFIDEIPEHLIEWERIGQASVGW
STSASGRSQSARGALRDDTPAFGSGRGSASALKGRTLALSVGDKVLHSTFGLGTVKATSGQGDNAKADVDFGSAGLKRLA
LRFAPLEKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=770, Percent_Identity=40.2597402597403, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI48994965, Length=709, Percent_Identity=36.1071932299013, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1787196, Length=363, Percent_Identity=25.068870523416, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=743, Percent_Identity=27.8600269179004, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324477, Length=680, Percent_Identity=22.5, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 89686; Mature: 89555

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPDLFSFFESAHERRSPVEPGVLPTYAPVTAGAASPADLLADLNEPQREAVLHAGSPV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHCCCCCE
LVVAGAGSGKTRVLTRRIAHLVGERGVHPGSILAITFTNKAAAEMKARVVDLVGNRAKPM
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCH
WVSTFHSAAVRILRTDIDRLGISRNFSIYDDTDAKRLITLVTRDQNLDPKKFQPRAIMNW
HHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHEEEEEECCCCCCCCCCCCHHHHHH
ISHQKNELVAPDQAVAAAKSGNDRINGDVYVEYQRRLRAANVLDFDDLIMYTVTVLRKFP
HHCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHH
EVRERYRRRFRHVLVDEYQDTNTAQYQFIRELCGDGPVELDDGARSVDPPELMVVGDSDQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCC
SIYAFRGATIRNIIDFEKDFPGARTILLEQNYRSTQTILTAANNLIKVNLNRPAKNLWTD
CEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEECCCCHHHHCCC
QGDGDKIVGYVADTEHDEAAWVARRIDELVDEGRTSYGQVAVFYRTNAQSRAFEDVFIRT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHC
GLPYRVVGGVRFYERKEVRDAVSYLRAIANPSDDVSLRRILNTPRRGIGARAEAAVEMFA
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
TARRISFWEALKRIDEIDSLATRSVNQIRAFVSLMDGHIAMVEQGKRADEIASSILKESG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHCCC
YITELENSKDPQDETRLENLIELVSVAQEFVARVHAVDIDEAEQGNVEDLELSGDDSLPA
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHH
FLEQIALVADSDQVPADSDGVVTLMTLHTAKGLEFDTVFITGFEDGVFPHMRSLADATEL
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHH
SEERRLAYVGITRARKQLFVSRASVRTMWGQAQYNPPSRFIDEIPEHLIEWERIGQASVG
HHHHCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
WSTSASGRSQSARGALRDDTPAFGSGRGSASALKGRTLALSVGDKVLHSTFGLGTVKATS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEECCHHHHHHHCCCCEEEECC
GQGDNAKADVDFGSAGLKRLALRFAPLEKL
CCCCCCEECCCCCHHHHHHHHHHHCCHHCC
>Mature Secondary Structure 
TTPDLFSFFESAHERRSPVEPGVLPTYAPVTAGAASPADLLADLNEPQREAVLHAGSPV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHCCCCCE
LVVAGAGSGKTRVLTRRIAHLVGERGVHPGSILAITFTNKAAAEMKARVVDLVGNRAKPM
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCH
WVSTFHSAAVRILRTDIDRLGISRNFSIYDDTDAKRLITLVTRDQNLDPKKFQPRAIMNW
HHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHEEEEEECCCCCCCCCCCCHHHHHH
ISHQKNELVAPDQAVAAAKSGNDRINGDVYVEYQRRLRAANVLDFDDLIMYTVTVLRKFP
HHCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHH
EVRERYRRRFRHVLVDEYQDTNTAQYQFIRELCGDGPVELDDGARSVDPPELMVVGDSDQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCC
SIYAFRGATIRNIIDFEKDFPGARTILLEQNYRSTQTILTAANNLIKVNLNRPAKNLWTD
CEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEECCCCHHHHCCC
QGDGDKIVGYVADTEHDEAAWVARRIDELVDEGRTSYGQVAVFYRTNAQSRAFEDVFIRT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHC
GLPYRVVGGVRFYERKEVRDAVSYLRAIANPSDDVSLRRILNTPRRGIGARAEAAVEMFA
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
TARRISFWEALKRIDEIDSLATRSVNQIRAFVSLMDGHIAMVEQGKRADEIASSILKESG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHCCC
YITELENSKDPQDETRLENLIELVSVAQEFVARVHAVDIDEAEQGNVEDLELSGDDSLPA
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHH
FLEQIALVADSDQVPADSDGVVTLMTLHTAKGLEFDTVFITGFEDGVFPHMRSLADATEL
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHH
SEERRLAYVGITRARKQLFVSRASVRTMWGQAQYNPPSRFIDEIPEHLIEWERIGQASVG
HHHHCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
WSTSASGRSQSARGALRDDTPAFGSGRGSASALKGRTLALSVGDKVLHSTFGLGTVKATS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEECCHHHHHHHCCCCEEEECC
GQGDNAKADVDFGSAGLKRLALRFAPLEKL
CCCCCCEECCCCCHHHHHHHHHHHCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]