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The map label for this gene is lcfB [H]

Identifier: 50843184

GI number: 50843184

Start: 1878129

End: 1879802

Strand: Reverse

Name: lcfB [H]

Synonym: PPA1724

Alternate gene names: 50843184

Gene position: 1879802-1878129 (Counterclockwise)

Preceding gene: 161621793

Following gene: 50843181

Centisome position: 73.42

GC content: 62.54

Gene sequence:

>1674_bases
ATGGGCTTTGATTCACAGCCGTTGCGCGCATACGTTGAACACGACTTCACCTATGTGGCAGGGCTGGAGCGTAACCTACA
CCGATTCGGCGATTCGCTTGCCATGATCGACCCGGCAACCGATCGCACCTGGACCTACCGTGAGCTGGCCGCTGACGTCG
AACGGTTCGCCGTTGTGCTTTCGCAACACGGGGTCGGTCCCGGGGACACCTTTGCTTTCGAGCTGTTCAATACCCCGCAG
TTCGCCATCTGTTACCTGGCCGCTCACCGTTTGGGGGCGGTGGGAACCGTCCTTAACTGTCGGCTCGCTCCCGGAGAATT
GGCTTGCGCCCTGCGTGACGCCCGCCCGAAAGTGTTGGTCCACGACGCCGAGATCACCTCTCGAGTGCTGCCCGCCATTG
CGGAGGTCGCCCCGGAGTCTGAGACCCCGACGCCCTTTCTCATCCAGGTGGCTCCGGCTGGCACCTCACCGGATCTGCCC
GACGGCGCCGTCGACTTCGACACCGCTTTGTCCCAGGCCGATGGGAGGGCCCCGGATCGTCCGTCGAACCTCAGCACCTA
TGACGAGGTCATCCGTCTCTACACCTCAGGGACGACCGGCCTGCCGAAAGGTGTTCCGCTGCCGTCCTTCGTGGAGGTCA
TGAGCGCGCACGATGTCATCATGCACTTCCCGCTCACTCCACAGGACCGCACCCTGAACATGACTCCGTGGTTCCACCGT
GGTGGCCTGCACTCTGGCGGTCCTAACCCCACCTTTTACGTCGGGGGATCGGTGGTGCCGATGCGCGAGTTCGACGCCGA
CACCGTGCTCGACTGGGTGGGCCGTTACCACATCACCTTCCTCATCGGCGCACCGACGGCCCTGGAGCGTCTGGCCCGCG
CCCAGGAGAAGAATGCGCGTGACCTGTCGGGATTGAAGGGCATCGTTACGATGGGATCCCCACTTGACGCCGGCTCGGCC
CGCCGGTATATGGAGGTTCTCACCCCCAACATCTCGAACGGGTACGGCACCACTGAGACCTTCTGGAACACCTTCCTGCG
TCCCTTCGATCTGCCGGGAATGGCCGGTACAGCTGGTCGGGCCAGCACTGATGACGACGTCGAGGTGGTCAAGGTTTTTG
ACGACCGTATCGCTGAGCCCGACGAGCTGGCCGCCAAGGACGACGTTGAGGTCGGTGAGGTCGCGATGCGCAGTCCCAAG
TGCGGTATGTCCTACTCCGGCGAGACTGGCTCGAAGGATCCGAAGTTCCATGCCGGATGGTTCTACCCGGGTGACCTCGC
GACTTGGGACGAGCATGAGTTCGTCACCATCGTCGGACGTAAGGACGAGATGATTATCAGCGGTGGTGAGAACGTGTTCT
CAACCCAGGTGGAGTCCGTGCTGGAATCCCACCCGAGCGTCCTGGAGTCGATCGTGGTGGGTCTGCCTGATCCGGAATGG
GGGCAGCTGGTCGTTGCCTACGTGGTGGCCGATCCTGACGCTGCTGCAGTGACAGCTGATGAGCTGGAGGCCCACTGCCT
GGCTAGTGAGGACCTGGCCCGGTTCAAACGACCTCGCGCCTACCGCTTCGTTAACAAGTTGCCGATGACGCCGACGGGCA
AGAAGAAGCATGTTGAGGCAACCGCTATGGCATGCCGGGAGGCCGACACATTCGTGCGTCCCCGTCATTACTGA

Upstream 100 bases:

>100_bases
ACAAACGCCAGCGTCACTTACCACCCACCACGCTGAGAAGCCGACCGTGACCGGCGTAACGTGGCAATTACGTTTAAACA
TTGGCAGAGAGGTGTCTTTC

Downstream 100 bases:

>100_bases
CGAGGACGCATCGTCCGGGTCGCAGGGACGCTCAGATTCCGAGAGCCTGGGACATCTCGTCTCGTAGGGTCGTCATGTCT
GCGTCAAGACGGGCCTTGGT

Product: putative fatty acid--CoA ligase (AMP-binding enzyme)

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MGFDSQPLRAYVEHDFTYVAGLERNLHRFGDSLAMIDPATDRTWTYRELAADVERFAVVLSQHGVGPGDTFAFELFNTPQ
FAICYLAAHRLGAVGTVLNCRLAPGELACALRDARPKVLVHDAEITSRVLPAIAEVAPESETPTPFLIQVAPAGTSPDLP
DGAVDFDTALSQADGRAPDRPSNLSTYDEVIRLYTSGTTGLPKGVPLPSFVEVMSAHDVIMHFPLTPQDRTLNMTPWFHR
GGLHSGGPNPTFYVGGSVVPMREFDADTVLDWVGRYHITFLIGAPTALERLARAQEKNARDLSGLKGIVTMGSPLDAGSA
RRYMEVLTPNISNGYGTTETFWNTFLRPFDLPGMAGTAGRASTDDDVEVVKVFDDRIAEPDELAAKDDVEVGEVAMRSPK
CGMSYSGETGSKDPKFHAGWFYPGDLATWDEHEFVTIVGRKDEMIISGGENVFSTQVESVLESHPSVLESIVVGLPDPEW
GQLVVAYVVADPDAAAVTADELEAHCLASEDLARFKRPRAYRFVNKLPMTPTGKKKHVEATAMACREADTFVRPRHY

Sequences:

>Translated_557_residues
MGFDSQPLRAYVEHDFTYVAGLERNLHRFGDSLAMIDPATDRTWTYRELAADVERFAVVLSQHGVGPGDTFAFELFNTPQ
FAICYLAAHRLGAVGTVLNCRLAPGELACALRDARPKVLVHDAEITSRVLPAIAEVAPESETPTPFLIQVAPAGTSPDLP
DGAVDFDTALSQADGRAPDRPSNLSTYDEVIRLYTSGTTGLPKGVPLPSFVEVMSAHDVIMHFPLTPQDRTLNMTPWFHR
GGLHSGGPNPTFYVGGSVVPMREFDADTVLDWVGRYHITFLIGAPTALERLARAQEKNARDLSGLKGIVTMGSPLDAGSA
RRYMEVLTPNISNGYGTTETFWNTFLRPFDLPGMAGTAGRASTDDDVEVVKVFDDRIAEPDELAAKDDVEVGEVAMRSPK
CGMSYSGETGSKDPKFHAGWFYPGDLATWDEHEFVTIVGRKDEMIISGGENVFSTQVESVLESHPSVLESIVVGLPDPEW
GQLVVAYVVADPDAAAVTADELEAHCLASEDLARFKRPRAYRFVNKLPMTPTGKKKHVEATAMACREADTFVRPRHY
>Mature_556_residues
GFDSQPLRAYVEHDFTYVAGLERNLHRFGDSLAMIDPATDRTWTYRELAADVERFAVVLSQHGVGPGDTFAFELFNTPQF
AICYLAAHRLGAVGTVLNCRLAPGELACALRDARPKVLVHDAEITSRVLPAIAEVAPESETPTPFLIQVAPAGTSPDLPD
GAVDFDTALSQADGRAPDRPSNLSTYDEVIRLYTSGTTGLPKGVPLPSFVEVMSAHDVIMHFPLTPQDRTLNMTPWFHRG
GLHSGGPNPTFYVGGSVVPMREFDADTVLDWVGRYHITFLIGAPTALERLARAQEKNARDLSGLKGIVTMGSPLDAGSAR
RYMEVLTPNISNGYGTTETFWNTFLRPFDLPGMAGTAGRASTDDDVEVVKVFDDRIAEPDELAAKDDVEVGEVAMRSPKC
GMSYSGETGSKDPKFHAGWFYPGDLATWDEHEFVTIVGRKDEMIISGGENVFSTQVESVLESHPSVLESIVVGLPDPEWG
QLVVAYVVADPDAAAVTADELEAHCLASEDLARFKRPRAYRFVNKLPMTPTGKKKHVEATAMACREADTFVRPRHY

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=540, Percent_Identity=25.1851851851852, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI42544132, Length=515, Percent_Identity=27.378640776699, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI38505220, Length=512, Percent_Identity=27.9296875, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI115511026, Length=525, Percent_Identity=26.2857142857143, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI28416953, Length=552, Percent_Identity=24.8188405797101, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI13375727, Length=550, Percent_Identity=26.1818181818182, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI122937307, Length=520, Percent_Identity=26.7307692307692, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI58082049, Length=519, Percent_Identity=25.0481695568401, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI157311624, Length=514, Percent_Identity=24.3190661478599, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157311622, Length=514, Percent_Identity=24.3190661478599, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI40807357, Length=533, Percent_Identity=24.577861163227, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI187761345, Length=540, Percent_Identity=23.3333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI187761343, Length=540, Percent_Identity=23.3333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI38524616, Length=541, Percent_Identity=24.5841035120148, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI145693145, Length=548, Percent_Identity=27.5547445255474, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788107, Length=541, Percent_Identity=25.6931608133087, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=25.4437869822485, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=25.7309941520468, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1790505, Length=560, Percent_Identity=23.0357142857143, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786529, Length=547, Percent_Identity=25.4113345521024, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789201, Length=350, Percent_Identity=24.2857142857143, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=491, Percent_Identity=25.050916496945, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=540, Percent_Identity=25.5555555555556, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=543, Percent_Identity=23.9410681399632, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=492, Percent_Identity=23.9837398373984, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=522, Percent_Identity=24.904214559387, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=522, Percent_Identity=24.904214559387, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=522, Percent_Identity=24.904214559387, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17559526, Length=507, Percent_Identity=25.4437869822485, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=509, Percent_Identity=24.3614931237721, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=535, Percent_Identity=24.1121495327103, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=520, Percent_Identity=22.6923076923077, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71982997, Length=562, Percent_Identity=26.1565836298932, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71983001, Length=562, Percent_Identity=26.1565836298932, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI115534440, Length=481, Percent_Identity=23.2848232848233, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=527, Percent_Identity=21.4421252371917, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=525, Percent_Identity=25.9047619047619, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323182, Length=552, Percent_Identity=25.7246376811594, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355181, Length=516, Percent_Identity=25.7751937984496, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI18859661, Length=541, Percent_Identity=27.3567467652495, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24581924, Length=536, Percent_Identity=27.0522388059701, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281364798, Length=573, Percent_Identity=22.1640488656195, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24583465, Length=573, Percent_Identity=22.1640488656195, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24583463, Length=573, Percent_Identity=22.1640488656195, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21356441, Length=520, Percent_Identity=22.5, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24653035, Length=520, Percent_Identity=22.6923076923077, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648260, Length=376, Percent_Identity=25.2659574468085, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365686, Length=499, Percent_Identity=22.8456913827655, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21356947, Length=532, Percent_Identity=21.2406015037594, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21358303, Length=334, Percent_Identity=23.0538922155689, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI161076582, Length=526, Percent_Identity=21.1026615969582, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 60818; Mature: 60687

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFDSQPLRAYVEHDFTYVAGLERNLHRFGDSLAMIDPATDRTWTYRELAADVERFAVVL
CCCCCHHHHHHHHCCCCEEHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHH
SQHGVGPGDTFAFELFNTPQFAICYLAAHRLGAVGTVLNCRLAPGELACALRDARPKVLV
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCEEEE
HDAEITSRVLPAIAEVAPESETPTPFLIQVAPAGTSPDLPDGAVDFDTALSQADGRAPDR
ECHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCC
PSNLSTYDEVIRLYTSGTTGLPKGVPLPSFVEVMSAHDVIMHFPLTPQDRTLNMTPWFHR
CCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCEECCCCCHHC
GGLHSGGPNPTFYVGGSVVPMREFDADTVLDWVGRYHITFLIGAPTALERLARAQEKNAR
CCCCCCCCCCEEEECCEEEEHHHCCHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCH
DLSGLKGIVTMGSPLDAGSARRYMEVLTPNISNGYGTTETFWNTFLRPFDLPGMAGTAGR
HHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
ASTDDDVEVVKVFDDRIAEPDELAAKDDVEVGEVAMRSPKCGMSYSGETGSKDPKFHAGW
CCCCCCHHHHEEHHHHCCCCHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEECE
FYPGDLATWDEHEFVTIVGRKDEMIISGGENVFSTQVESVLESHPSVLESIVVGLPDPEW
EECCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCC
GQLVVAYVVADPDAAAVTADELEAHCLASEDLARFKRPRAYRFVNKLPMTPTGKKKHVEA
CCEEEEEEEECCCCCEEEHHHHHHHHHCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHH
TAMACREADTFVRPRHY
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GFDSQPLRAYVEHDFTYVAGLERNLHRFGDSLAMIDPATDRTWTYRELAADVERFAVVL
CCCCHHHHHHHHCCCCEEHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHH
SQHGVGPGDTFAFELFNTPQFAICYLAAHRLGAVGTVLNCRLAPGELACALRDARPKVLV
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCEEEE
HDAEITSRVLPAIAEVAPESETPTPFLIQVAPAGTSPDLPDGAVDFDTALSQADGRAPDR
ECHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCC
PSNLSTYDEVIRLYTSGTTGLPKGVPLPSFVEVMSAHDVIMHFPLTPQDRTLNMTPWFHR
CCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCEECCCCCHHC
GGLHSGGPNPTFYVGGSVVPMREFDADTVLDWVGRYHITFLIGAPTALERLARAQEKNAR
CCCCCCCCCCEEEECCEEEEHHHCCHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCH
DLSGLKGIVTMGSPLDAGSARRYMEVLTPNISNGYGTTETFWNTFLRPFDLPGMAGTAGR
HHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
ASTDDDVEVVKVFDDRIAEPDELAAKDDVEVGEVAMRSPKCGMSYSGETGSKDPKFHAGW
CCCCCCHHHHEEHHHHCCCCHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEECE
FYPGDLATWDEHEFVTIVGRKDEMIISGGENVFSTQVESVLESHPSVLESIVVGLPDPEW
EECCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCC
GQLVVAYVVADPDAAAVTADELEAHCLASEDLARFKRPRAYRFVNKLPMTPTGKKKHVEA
CCEEEEEEEECCCCCEEEHHHHHHHHHCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHH
TAMACREADTFVRPRHY
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]