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The map label for this gene is metG

Identifier: 161621793

GI number: 161621793

Start: 1883298

End: 1885076

Strand: Reverse

Name: metG

Synonym: PPA1728

Alternate gene names: 161621793

Gene position: 1885076-1883298 (Counterclockwise)

Preceding gene: 50843189

Following gene: 50843184

Centisome position: 73.63

GC content: 58.35

Gene sequence:

>1779_bases
ATGTGTGCCAACGTTCTTGCCGCCGTCGCGTGGCCCTACGCTAATGGGCCTCGACATATCGGCCATGTTTCCGGATTCGG
TGTCCCCTCTGACGTCTTTGCCCGGTACATGCGAATGTCGGGCCACCGAGTCCTCATGGTTTCCGGCTCGGATTGTCATG
GCACCGCTATTTCAGTCAAGGCTGATCAGGAGGGCGTGACGGCTCAGGAGTGTGCTGAGAAGTACCACCGCATCATCGCG
GCAGACTTGCAGGGGCTCGGATTGTCCTACGATCTGTACACGTCGACGCTGACCGACAACCACGCTCACGTCACCCAGGA
GATCTTTACTCGCCTCCACGAAAACGGCTACGTCGTCAAGAGGTCCGAGATGGGGGCGTTTGAGCCATCGACCGGACGTA
CCCTTCCGGACCGTTACATTGAGGGCACCTGCCCGGTGTGTGGATACGACGACGCCCGCGGTGACCAGTGCGACAACTGT
GGCCGTCAGCTCGACCCGGCCGACCTCATCGGCCCCAGGTCAAAGACCACAGGGGCCGCTCCCGAGTTTCGCGAGACCGA
GCATTTCTTCCTTGACCTTCCTGCTCTGGCCGAGTCGTTGGCATCGTGGATTGACACTCGCACCGATTGGCGTCCGAATG
TCTTGAAGTTTTCCCACAACCTGCTTGAGGAGTTGCGTCCGCGCGCTATTACCCGCGATCTCGACTGGGGCATTCGCGTG
CCGGTAGAAGGCTGGCAGGACAACCCGATGAAGTCGATCTACGTGTGGTTCGACGCTGTTATCGGATACCTGTCTGCCTC
GATTGAGTGGGCGCGCCGTATTGGTAGGCCTGATGCGTGGCGTGAGTTCTGGAATGACGAGGACGCTCGTTCGTACTACT
TCATGGGCAAGGACAACATCGTGTTCCACTCGGTCATTTGGCCCGGCATCCTGTTGGGTACAAATGGGCGTGGCGATAAG
GGTGGCGAGCCATCCGAGGAACTTGGAACCCTTAACCTTCCCACCGAGATTGTTTCCAGCGAGTTTCTGACGATGAGTGG
TTCAAAGGTGTCGAACTCTCGTGGTGCCACTATTTTTGTCGGAGATTTCCTGCATGAGTTCGGTCCCGACGCCCTGCGCT
ACTTCATTGCGGTTGCCGGTCCGGAAAATCAGGACACGGACTTCACCTGGGAAGAGTTTGTCCGCCGGGTGAACTTTGAG
CTCGCCAACGAGTGGGGCAACCTCGTTAACAGGTCGATTTCGATGGCGTTCAAGAATTGCCACGAGATTCCGGCTGCTGG
TGAGTTGACAGACGCTGACCGTGAGTTGCTGGATGCCGCTGCTGCTGGGTTTGACACCGTGGGCGGGTTGCTTGCCCACG
CCAGATTCAAGGCGGCTATTACTGAGGCGATGAGGATCGTTGGGCTAGCTAATGCCTATATCTCTGCTGAGGAACCGTGG
AAGCTCAAGGACAATCCTGAGCGTCGTGACACCGTGCTGCATGTGGCTTTGCAGGTCGTGAGTGACGTTAACACCATGAT
GACCCCGTTCATGCCTCATTCGGCGCAGAAGATTTATGAGGCCCTTGGTGGTGAGGGTGTGTGGGCGGCCCAGCCTGAGC
TCATTGAGACCGATGGGGCACCGATCCTCATGGGGGATTACGCCACCGAGCAGGCGTCATGGGGCCGTCATGAAATCACG
GTAGGTACTCCGCTGTCTAAACCTTCGCCCATCTTCCGGAAGCTCGACGCTAAGCTCGCCCAAACCGGTCCGCAATGGGC
CCCGGTCAACCCGCAGTGA

Upstream 100 bases:

>100_bases
CGGGTCATGAACCACGATGACCTCGATCGGTACATAGCATCGACCTACGCTGCTTCAATCGTTGACATCCTCGATGTCGG
CTACCGATAGAGTTAACCTC

Downstream 100 bases:

>100_bases
GTCAGCTTGCCTGCGCCCGTCTCTGTAACTCGCAGACGATGAGGGCGCAGGCTTCGGCGTCGGAGTCGGCTCGATGATGG
TTGAGCTGGTAATTCGGGGC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MCANVLAAVAWPYANGPRHIGHVSGFGVPSDVFARYMRMSGHRVLMVSGSDCHGTAISVKADQEGVTAQECAEKYHRIIA
ADLQGLGLSYDLYTSTLTDNHAHVTQEIFTRLHENGYVVKRSEMGAFEPSTGRTLPDRYIEGTCPVCGYDDARGDQCDNC
GRQLDPADLIGPRSKTTGAAPEFRETEHFFLDLPALAESLASWIDTRTDWRPNVLKFSHNLLEELRPRAITRDLDWGIRV
PVEGWQDNPMKSIYVWFDAVIGYLSASIEWARRIGRPDAWREFWNDEDARSYYFMGKDNIVFHSVIWPGILLGTNGRGDK
GGEPSEELGTLNLPTEIVSSEFLTMSGSKVSNSRGATIFVGDFLHEFGPDALRYFIAVAGPENQDTDFTWEEFVRRVNFE
LANEWGNLVNRSISMAFKNCHEIPAAGELTDADRELLDAAAAGFDTVGGLLAHARFKAAITEAMRIVGLANAYISAEEPW
KLKDNPERRDTVLHVALQVVSDVNTMMTPFMPHSAQKIYEALGGEGVWAAQPELIETDGAPILMGDYATEQASWGRHEIT
VGTPLSKPSPIFRKLDAKLAQTGPQWAPVNPQ

Sequences:

>Translated_592_residues
MCANVLAAVAWPYANGPRHIGHVSGFGVPSDVFARYMRMSGHRVLMVSGSDCHGTAISVKADQEGVTAQECAEKYHRIIA
ADLQGLGLSYDLYTSTLTDNHAHVTQEIFTRLHENGYVVKRSEMGAFEPSTGRTLPDRYIEGTCPVCGYDDARGDQCDNC
GRQLDPADLIGPRSKTTGAAPEFRETEHFFLDLPALAESLASWIDTRTDWRPNVLKFSHNLLEELRPRAITRDLDWGIRV
PVEGWQDNPMKSIYVWFDAVIGYLSASIEWARRIGRPDAWREFWNDEDARSYYFMGKDNIVFHSVIWPGILLGTNGRGDK
GGEPSEELGTLNLPTEIVSSEFLTMSGSKVSNSRGATIFVGDFLHEFGPDALRYFIAVAGPENQDTDFTWEEFVRRVNFE
LANEWGNLVNRSISMAFKNCHEIPAAGELTDADRELLDAAAAGFDTVGGLLAHARFKAAITEAMRIVGLANAYISAEEPW
KLKDNPERRDTVLHVALQVVSDVNTMMTPFMPHSAQKIYEALGGEGVWAAQPELIETDGAPILMGDYATEQASWGRHEIT
VGTPLSKPSPIFRKLDAKLAQTGPQWAPVNPQ
>Mature_592_residues
MCANVLAAVAWPYANGPRHIGHVSGFGVPSDVFARYMRMSGHRVLMVSGSDCHGTAISVKADQEGVTAQECAEKYHRIIA
ADLQGLGLSYDLYTSTLTDNHAHVTQEIFTRLHENGYVVKRSEMGAFEPSTGRTLPDRYIEGTCPVCGYDDARGDQCDNC
GRQLDPADLIGPRSKTTGAAPEFRETEHFFLDLPALAESLASWIDTRTDWRPNVLKFSHNLLEELRPRAITRDLDWGIRV
PVEGWQDNPMKSIYVWFDAVIGYLSASIEWARRIGRPDAWREFWNDEDARSYYFMGKDNIVFHSVIWPGILLGTNGRGDK
GGEPSEELGTLNLPTEIVSSEFLTMSGSKVSNSRGATIFVGDFLHEFGPDALRYFIAVAGPENQDTDFTWEEFVRRVNFE
LANEWGNLVNRSISMAFKNCHEIPAAGELTDADRELLDAAAAGFDTVGGLLAHARFKAAITEAMRIVGLANAYISAEEPW
KLKDNPERRDTVLHVALQVVSDVNTMMTPFMPHSAQKIYEALGGEGVWAAQPELIETDGAPILMGDYATEQASWGRHEIT
VGTPLSKPSPIFRKLDAKLAQTGPQWAPVNPQ

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=530, Percent_Identity=34.9056603773585, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI24308436, Length=544, Percent_Identity=25.3676470588235, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1788432, Length=535, Percent_Identity=32.1495327102804, Blast_Score=290, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17540794, Length=592, Percent_Identity=32.6013513513513, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI212645096, Length=529, Percent_Identity=23.2514177693762, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71992781, Length=365, Percent_Identity=24.3835616438356, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=534, Percent_Identity=35.2059925093633, Blast_Score=328, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24655311, Length=586, Percent_Identity=32.7645051194539, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21358483, Length=541, Percent_Identity=23.6598890942699, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_PROAC (Q6A707)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056415.2
- ProteinModelPortal:   Q6A707
- GeneID:   2931533
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1728
- NMPDR:   fig|267747.1.peg.1692
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- ProtClustDB:   PRK12268
- BioCyc:   PACN267747:PPA1728-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 65614; Mature: 65614

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 361-361

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCANVLAAVAWPYANGPRHIGHVSGFGVPSDVFARYMRMSGHRVLMVSGSDCHGTAISVK
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
ADQEGVTAQECAEKYHRIIAADLQGLGLSYDLYTSTLTDNHAHVTQEIFTRLHENGYVVK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHHHHHCCCCEEEE
RSEMGAFEPSTGRTLPDRYIEGTCPVCGYDDARGDQCDNCGRQLDPADLIGPRSKTTGAA
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHCCCCCCCCCCC
PEFRETEHFFLDLPALAESLASWIDTRTDWRPNVLKFSHNLLEELRPRAITRDLDWGIRV
CCCCCCCHHEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCEEC
PVEGWQDNPMKSIYVWFDAVIGYLSASIEWARRIGRPDAWREFWNDEDARSYYFMGKDNI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCE
VFHSVIWPGILLGTNGRGDKGGEPSEELGTLNLPTEIVSSEFLTMSGSKVSNSRGATIFV
EEEEECCCCEEECCCCCCCCCCCCHHHCCCCCCCHHHHCCHHEEECCCCCCCCCCCEEEE
GDFLHEFGPDALRYFIAVAGPENQDTDFTWEEFVRRVNFELANEWGNLVNRSISMAFKNC
HHHHHHHCHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEIPAAGELTDADRELLDAAAAGFDTVGGLLAHARFKAAITEAMRIVGLANAYISAEEPW
HCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KLKDNPERRDTVLHVALQVVSDVNTMMTPFMPHSAQKIYEALGGEGVWAAQPELIETDGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEECCCCCEEECCCC
PILMGDYATEQASWGRHEITVGTPLSKPSPIFRKLDAKLAQTGPQWAPVNPQ
CEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MCANVLAAVAWPYANGPRHIGHVSGFGVPSDVFARYMRMSGHRVLMVSGSDCHGTAISVK
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
ADQEGVTAQECAEKYHRIIAADLQGLGLSYDLYTSTLTDNHAHVTQEIFTRLHENGYVVK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHHHHHCCCCEEEE
RSEMGAFEPSTGRTLPDRYIEGTCPVCGYDDARGDQCDNCGRQLDPADLIGPRSKTTGAA
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHCCCCCCCCCCC
PEFRETEHFFLDLPALAESLASWIDTRTDWRPNVLKFSHNLLEELRPRAITRDLDWGIRV
CCCCCCCHHEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCEEC
PVEGWQDNPMKSIYVWFDAVIGYLSASIEWARRIGRPDAWREFWNDEDARSYYFMGKDNI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCE
VFHSVIWPGILLGTNGRGDKGGEPSEELGTLNLPTEIVSSEFLTMSGSKVSNSRGATIFV
EEEEECCCCEEECCCCCCCCCCCCHHHCCCCCCCHHHHCCHHEEECCCCCCCCCCCEEEE
GDFLHEFGPDALRYFIAVAGPENQDTDFTWEEFVRRVNFELANEWGNLVNRSISMAFKNC
HHHHHHHCHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEIPAAGELTDADRELLDAAAAGFDTVGGLLAHARFKAAITEAMRIVGLANAYISAEEPW
HCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KLKDNPERRDTVLHVALQVVSDVNTMMTPFMPHSAQKIYEALGGEGVWAAQPELIETDGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEECCCCCEEECCCC
PILMGDYATEQASWGRHEITVGTPLSKPSPIFRKLDAKLAQTGPQWAPVNPQ
CEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA