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The map label for this gene is birA [H]

Identifier: 50843167

GI number: 50843167

Start: 1859191

End: 1860030

Strand: Reverse

Name: birA [H]

Synonym: PPA1705

Alternate gene names: 50843167

Gene position: 1860030-1859191 (Counterclockwise)

Preceding gene: 50843172

Following gene: 50843166

Centisome position: 72.65

GC content: 59.64

Gene sequence:

>840_bases
GTGCCGTCCACACCTTCGCCTATTGCTGATCGCGTTCTTGAGCTTGCCGGGGACACCCTTTTTACGGGGCCTCGTGGGGG
AGGAGTTACCTGCATTGACTCCACCGGTTCCACTAACGCCGACATGGCTGCTTTCGTGCGAGCAGGGGGAACGTCTTTCT
GCCTACTCGTTGCTGACCACCAAGAGGGCGGGCGTGGACGGTTCACGCGCAGTTGGCAGGATGTCCCCGGTACGAGTTTG
GCGATCTCAGCGTTGGTGCCCAATGATCGTCCGTCGCAGGACTGGGGCTGGCTGTCGATGGTTGCGGGGCTCGCTGTTGT
CAAGGTCATCAAGGAGGTCGGTGGGGCTGACCGTTCCCGAGTGACGCTGAAGTGGCCCAATGATGTGCTCGTGGATCTGG
ACACTGACCAGGGCGGCAAAGTGTGCGGAATTCTCTCAGAACGCGTTGACGGTCCAGCCGGTCCGCACGCCGTCATCGGA
ATCGGCATCAACGTTTCGATGGGGCGTGACGAATTGCCCCTGCCGACGGCGACCTCTCTGGCTCTGTGTGGGTTGAACCA
CGACAAGAATGAGTTGCTGGCCAGCCTTCTCATCCACCTTGACGAGCTATTAACAGTGTGGTTGGAGACCGGAACGGTGC
GGGATCAGTATGTGGCCCGCTGTGACACCATTGGTACTCCGGTCCGTCTGACCTTCGACCCAGAAATCGTGGGTGGTGGT
GAGGGGGCCATTGAGGGCATCGGTGTCGACGTTGACGTTGATGGCGCTATCGTGGTGGAAACTTCTGACGGGCGTCGCAG
TTTCAACGCTGCTGACGTTCATCATTTGCGAACCAGGTGA

Upstream 100 bases:

>100_bases
GTCGTCTCTTTCAGCGATAACATATCTATTTAGGATAACAATAGGGAACCCGGCGTTGGTCCGGCCGTCGCGCGTGGGTA
CTTTTGGGTAGTCTGAGCAG

Downstream 100 bases:

>100_bases
GTTCCGCTACGGCGTCCTGAGCGTTCCCACCATCTAGACTGCTGACTATGACGACCCACATCTTGGCCCTTGGTGGTGGC
GGTTTTTCGATGTCGAACCG

Product: putative BirA bifunctional protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MPSTPSPIADRVLELAGDTLFTGPRGGGVTCIDSTGSTNADMAAFVRAGGTSFCLLVADHQEGGRGRFTRSWQDVPGTSL
AISALVPNDRPSQDWGWLSMVAGLAVVKVIKEVGGADRSRVTLKWPNDVLVDLDTDQGGKVCGILSERVDGPAGPHAVIG
IGINVSMGRDELPLPTATSLALCGLNHDKNELLASLLIHLDELLTVWLETGTVRDQYVARCDTIGTPVRLTFDPEIVGGG
EGAIEGIGVDVDVDGAIVVETSDGRRSFNAADVHHLRTR

Sequences:

>Translated_279_residues
MPSTPSPIADRVLELAGDTLFTGPRGGGVTCIDSTGSTNADMAAFVRAGGTSFCLLVADHQEGGRGRFTRSWQDVPGTSL
AISALVPNDRPSQDWGWLSMVAGLAVVKVIKEVGGADRSRVTLKWPNDVLVDLDTDQGGKVCGILSERVDGPAGPHAVIG
IGINVSMGRDELPLPTATSLALCGLNHDKNELLASLLIHLDELLTVWLETGTVRDQYVARCDTIGTPVRLTFDPEIVGGG
EGAIEGIGVDVDVDGAIVVETSDGRRSFNAADVHHLRTR
>Mature_278_residues
PSTPSPIADRVLELAGDTLFTGPRGGGVTCIDSTGSTNADMAAFVRAGGTSFCLLVADHQEGGRGRFTRSWQDVPGTSLA
ISALVPNDRPSQDWGWLSMVAGLAVVKVIKEVGGADRSRVTLKWPNDVLVDLDTDQGGKVCGILSERVDGPAGPHAVIGI
GINVSMGRDELPLPTATSLALCGLNHDKNELLASLLIHLDELLTVWLETGTVRDQYVARCDTIGTPVRLTFDPEIVGGGE
GAIEGIGVDVDVDGAIVVETSDGRRSFNAADVHHLRTR

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=272, Percent_Identity=31.6176470588235, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 29328; Mature: 29197

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTPSPIADRVLELAGDTLFTGPRGGGVTCIDSTGSTNADMAAFVRAGGTSFCLLVADH
CCCCCCHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCHHHHHEECCCCEEEEEEEEC
QEGGRGRFTRSWQDVPGTSLAISALVPNDRPSQDWGWLSMVAGLAVVKVIKEVGGADRSR
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VTLKWPNDVLVDLDTDQGGKVCGILSERVDGPAGPHAVIGIGINVSMGRDELPLPTATSL
EEEECCCCEEEEECCCCCCCEEEEHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCE
ALCGLNHDKNELLASLLIHLDELLTVWLETGTVRDQYVARCDTIGTPVRLTFDPEIVGGG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCEEECCC
EGAIEGIGVDVDVDGAIVVETSDGRRSFNAADVHHLRTR
CCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
PSTPSPIADRVLELAGDTLFTGPRGGGVTCIDSTGSTNADMAAFVRAGGTSFCLLVADH
CCCCCHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCHHHHHEECCCCEEEEEEEEC
QEGGRGRFTRSWQDVPGTSLAISALVPNDRPSQDWGWLSMVAGLAVVKVIKEVGGADRSR
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VTLKWPNDVLVDLDTDQGGKVCGILSERVDGPAGPHAVIGIGINVSMGRDELPLPTATSL
EEEECCCCEEEEECCCCCCCEEEEHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCE
ALCGLNHDKNELLASLLIHLDELLTVWLETGTVRDQYVARCDTIGTPVRLTFDPEIVGGG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCEEECCC
EGAIEGIGVDVDVDGAIVVETSDGRRSFNAADVHHLRTR
CCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]