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The map label for this gene is rph

Identifier: 50843137

GI number: 50843137

Start: 1827509

End: 1828273

Strand: Reverse

Name: rph

Synonym: PPA1674

Alternate gene names: 50843137

Gene position: 1828273-1827509 (Counterclockwise)

Preceding gene: 50843138

Following gene: 50843136

Centisome position: 71.41

GC content: 60.65

Gene sequence:

>765_bases
GTGTCGGTGTGGTCCACTATCCTTGGCAAGGTGAGTGAGACTTCTCGAATTGACGGACGCCGCCTGGACCAGTTGCGAGA
CGTCAGGATTGAACGCGGCTGGTTGTCTCAGGCTGAGGGTTCGGTGTTGGTGAGCTTTGGTCGCACCACTGTGCTGTGTA
ATGCCTCGGTCACTGAGGGTGTACCGCGTTGGCGTAAGGGATCCGGGCTGGGATGGGTCACCGCCGAGTATGAGATGCTT
CCGCGTGCCACCAATGAGCGGTCTGGACGCGAGTCGCGCAAGGGCAAGGTGGGAGGCCGTACTCATGAGATTTCCCGCCT
GGTAGGCCGTTCCCTGCGTGCCGTCGTTGACGACAAGGCGCTGGGAGAAAACACCATTATCCTCGACTGCGACGTGCTGC
AGGCCGACGGTGGTACCCGCACCGCTTCCATTACCGGCGCTTATGTTGCCCTTATTGACGCCGTCAACTGGCTGCGGGGG
CGTGGCGGACTGGTTAGCGAGCCCATCATCGGATCAGTGCAGGCTATCTCGGTTGGTGTGGTCGACGGCATCCCGATGCT
CGACCTCGCTTATAAGGAGGATTCGCGCGCCGATACCGACATGAACGTCGTCATGAGCGGCAATGGAGATTTCGTCGAGA
TCCAGGGAACGGCCGAGGGCACCCCCTTTAACCGCAACTTGCTCAATGAGTTGCTCGATCTAGCCGCCGGCGGCTGCGCA
ACCCTCAAGCAGGCCCAGTCCGAGGCTTTGGGGGTGACGTTATGA

Upstream 100 bases:

>100_bases
CCACGATGTGCAGTCGGTGCATGCTGATGCGCACTCCCTCGGTGATCGCGCGGTTGTACGAGACTGAACTCCTGCGCGAC
GGTGTTGTCACAGCTGCTGT

Downstream 100 bases:

>100_bases
GCCGGATTGTGCTGGCATCCAATAATGCTAAGAAGCTGGTCGAGCTGCGTCGCACTTTCGAGGGAGCAGACACCGAGGTC
GAAATCGTCGGTTTGTCGGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSVWSTILGKVSETSRIDGRRLDQLRDVRIERGWLSQAEGSVLVSFGRTTVLCNASVTEGVPRWRKGSGLGWVTAEYEML
PRATNERSGRESRKGKVGGRTHEISRLVGRSLRAVVDDKALGENTIILDCDVLQADGGTRTASITGAYVALIDAVNWLRG
RGGLVSEPIIGSVQAISVGVVDGIPMLDLAYKEDSRADTDMNVVMSGNGDFVEIQGTAEGTPFNRNLLNELLDLAAGGCA
TLKQAQSEALGVTL

Sequences:

>Translated_254_residues
MSVWSTILGKVSETSRIDGRRLDQLRDVRIERGWLSQAEGSVLVSFGRTTVLCNASVTEGVPRWRKGSGLGWVTAEYEML
PRATNERSGRESRKGKVGGRTHEISRLVGRSLRAVVDDKALGENTIILDCDVLQADGGTRTASITGAYVALIDAVNWLRG
RGGLVSEPIIGSVQAISVGVVDGIPMLDLAYKEDSRADTDMNVVMSGNGDFVEIQGTAEGTPFNRNLLNELLDLAAGGCA
TLKQAQSEALGVTL
>Mature_253_residues
SVWSTILGKVSETSRIDGRRLDQLRDVRIERGWLSQAEGSVLVSFGRTTVLCNASVTEGVPRWRKGSGLGWVTAEYEMLP
RATNERSGRESRKGKVGGRTHEISRLVGRSLRAVVDDKALGENTIILDCDVLQADGGTRTASITGAYVALIDAVNWLRGR
GGLVSEPIIGSVQAISVGVVDGIPMLDLAYKEDSRADTDMNVVMSGNGDFVEIQGTAEGTPFNRNLLNELLDLAAGGCAT
LKQAQSEALGVTL

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=61.4285714285714, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71981632, Length=189, Percent_Identity=32.8042328042328, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27175; Mature: 27044

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVWSTILGKVSETSRIDGRRLDQLRDVRIERGWLSQAEGSVLVSFGRTTVLCNASVTEG
CCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCHHHHC
VPRWRKGSGLGWVTAEYEMLPRATNERSGRESRKGKVGGRTHEISRLVGRSLRAVVDDKA
CCCCCCCCCCEEEEEHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LGENTIILDCDVLQADGGTRTASITGAYVALIDAVNWLRGRGGLVSEPIIGSVQAISVGV
CCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
VDGIPMLDLAYKEDSRADTDMNVVMSGNGDFVEIQGTAEGTPFNRNLLNELLDLAAGGCA
ECCCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHH
TLKQAQSEALGVTL
HHHHHHHHHCCCCC
>Mature Secondary Structure 
SVWSTILGKVSETSRIDGRRLDQLRDVRIERGWLSQAEGSVLVSFGRTTVLCNASVTEG
CHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCHHHHC
VPRWRKGSGLGWVTAEYEMLPRATNERSGRESRKGKVGGRTHEISRLVGRSLRAVVDDKA
CCCCCCCCCCEEEEEHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LGENTIILDCDVLQADGGTRTASITGAYVALIDAVNWLRGRGGLVSEPIIGSVQAISVGV
CCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
VDGIPMLDLAYKEDSRADTDMNVVMSGNGDFVEIQGTAEGTPFNRNLLNELLDLAAGGCA
ECCCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHH
TLKQAQSEALGVTL
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA