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The map label for this gene is thyA

Identifier: 50843135

GI number: 50843135

Start: 1826088

End: 1826897

Strand: Reverse

Name: thyA

Synonym: PPA1672

Alternate gene names: 50843135

Gene position: 1826897-1826088 (Counterclockwise)

Preceding gene: 50843136

Following gene: 50843134

Centisome position: 71.36

GC content: 59.14

Gene sequence:

>810_bases
ATGCAGATCTACCTCGACTTGTTGCGGCGGATCCTTGACGAGGGCGTGCGCCGCGAGGACCGTACCGGAACGGGGACGGT
CAGCGTCTTCGGTCACCAGATGGTCTTTGATCTGCGCGAGGGATTCCCGCTGGTAACGACGAAGAAGATTTACACCCGCA
GCGTGTTCGGGGAGTTGCTGTGGTTTCTGCGCGGGGATACGAATGTCGGCTGGCTGCATGATAATAACATCCACATTTGG
GACGAGTGGGCCGACGAGAACGGTGATTTAGGGCCGGTCTACGGGCATCAGTGGCGTTCTTGGCCCGACGACGATGGCAC
AATTGATCAGATCGCCAACGTGGTTGAGCAGATTCGCACCAATCCGTGGTCGCGTCGTCACATCGTCTCGGCATGGAATG
TGGCCGAGATCAAGGAGATGGCCCTGCCGCCGTGCCACACTTTGTTCCAGTTTTACGTCACGCCGGATGATCGGGGAACC
CCGACGTGGTTGAGCTGCCAGCTGTACCAGCGCTCCGGTGACACCTTCCTCGGAGTGCCGTTCAATATCGCGTCATATGC
CCTGCTCACCCATCTGGTGGCCTCGGTGACCGGGCTCAAGCCGTTGCGGTTCGTCCACACCTTGGGTGATGCCCATGTGT
ACCTCAATCACCTCGACCAGGTTCATGAGCAGTTGAAACGTAAGCCGCGGCACCGGCCAACGCTCACCGTCAACCCGGGT
ATCCGTGACATTGACGGGTTTGAGCTGTCCGATATCACCCTGACTGGTTATGACCCGTATCCGGCGCTGCCTGCTCCGAT
TGCGGTCTGA

Upstream 100 bases:

>100_bases
TGCTGAGATGACTCCTGAGGTCAAGGACGCCATCAGCCACCGAGGACAAGCTGTTCGCGCGATTGTTCCGGCGGTCGTCG
CCCATTTGGAGGGGCGCTGA

Downstream 100 bases:

>100_bases
TCGGGTGCCGGGATCAAGTTTCCACCAGGCCGCAGGAGGATTGATGAAGGTCGTCGCTATCGCCGCCGTTGCTGATAACG
GCGTCATCGGGTCGGGGCAG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MQIYLDLLRRILDEGVRREDRTGTGTVSVFGHQMVFDLREGFPLVTTKKIYTRSVFGELLWFLRGDTNVGWLHDNNIHIW
DEWADENGDLGPVYGHQWRSWPDDDGTIDQIANVVEQIRTNPWSRRHIVSAWNVAEIKEMALPPCHTLFQFYVTPDDRGT
PTWLSCQLYQRSGDTFLGVPFNIASYALLTHLVASVTGLKPLRFVHTLGDAHVYLNHLDQVHEQLKRKPRHRPTLTVNPG
IRDIDGFELSDITLTGYDPYPALPAPIAV

Sequences:

>Translated_269_residues
MQIYLDLLRRILDEGVRREDRTGTGTVSVFGHQMVFDLREGFPLVTTKKIYTRSVFGELLWFLRGDTNVGWLHDNNIHIW
DEWADENGDLGPVYGHQWRSWPDDDGTIDQIANVVEQIRTNPWSRRHIVSAWNVAEIKEMALPPCHTLFQFYVTPDDRGT
PTWLSCQLYQRSGDTFLGVPFNIASYALLTHLVASVTGLKPLRFVHTLGDAHVYLNHLDQVHEQLKRKPRHRPTLTVNPG
IRDIDGFELSDITLTGYDPYPALPAPIAV
>Mature_269_residues
MQIYLDLLRRILDEGVRREDRTGTGTVSVFGHQMVFDLREGFPLVTTKKIYTRSVFGELLWFLRGDTNVGWLHDNNIHIW
DEWADENGDLGPVYGHQWRSWPDDDGTIDQIANVVEQIRTNPWSRRHIVSAWNVAEIKEMALPPCHTLFQFYVTPDDRGT
PTWLSCQLYQRSGDTFLGVPFNIASYALLTHLVASVTGLKPLRFVHTLGDAHVYLNHLDQVHEQLKRKPRHRPTLTVNPG
IRDIDGFELSDITLTGYDPYPALPAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=287, Percent_Identity=48.4320557491289, Blast_Score=268, Evalue=5e-72,
Organism=Escherichia coli, GI1789191, Length=270, Percent_Identity=57.4074074074074, Blast_Score=327, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI71993377, Length=288, Percent_Identity=48.9583333333333, Blast_Score=260, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=290, Percent_Identity=41.3793103448276, Blast_Score=205, Evalue=6e-54,
Organism=Drosophila melanogaster, GI17137556, Length=287, Percent_Identity=52.2648083623693, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_PROAC (Q6A761)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056362.1
- ProteinModelPortal:   Q6A761
- SMR:   Q6A761
- GeneID:   2933035
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1672
- NMPDR:   fig|267747.1.peg.1639
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   PACN267747:PPA1672-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30838; Mature: 30838

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYLDLLRRILDEGVRREDRTGTGTVSVFGHQMVFDLREGFPLVTTKKIYTRSVFGELL
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
WFLRGDTNVGWLHDNNIHIWDEWADENGDLGPVYGHQWRSWPDDDGTIDQIANVVEQIRT
HHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
NPWSRRHIVSAWNVAEIKEMALPPCHTLFQFYVTPDDRGTPTWLSCQLYQRSGDTFLGVP
CCCCCCHHHHHCCHHHHHHHCCCCHHHEEEEEECCCCCCCCCEEEEEEEECCCCEEEECC
FNIASYALLTHLVASVTGLKPLRFVHTLGDAHVYLNHLDQVHEQLKRKPRHRPTLTVNPG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
IRDIDGFELSDITLTGYDPYPALPAPIAV
CCCCCCCEECEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MQIYLDLLRRILDEGVRREDRTGTGTVSVFGHQMVFDLREGFPLVTTKKIYTRSVFGELL
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
WFLRGDTNVGWLHDNNIHIWDEWADENGDLGPVYGHQWRSWPDDDGTIDQIANVVEQIRT
HHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
NPWSRRHIVSAWNVAEIKEMALPPCHTLFQFYVTPDDRGTPTWLSCQLYQRSGDTFLGVP
CCCCCCHHHHHCCHHHHHHHCCCCHHHEEEEEECCCCCCCCCEEEEEEEECCCCEEEECC
FNIASYALLTHLVASVTGLKPLRFVHTLGDAHVYLNHLDQVHEQLKRKPRHRPTLTVNPG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
IRDIDGFELSDITLTGYDPYPALPAPIAV
CCCCCCCEECEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA