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The map label for this gene is rlmN

Identifier: 50842992

GI number: 50842992

Start: 1637705

End: 1638922

Strand: Reverse

Name: rlmN

Synonym: PPA1514

Alternate gene names: 50842992

Gene position: 1638922-1637705 (Counterclockwise)

Preceding gene: 50842993

Following gene: 50842991

Centisome position: 64.01

GC content: 62.15

Gene sequence:

>1218_bases
ATGTCATCGACTGGAAGTTCCGTCAGCACGTCAGGTCTTGTTCTGCCCTCCACGCCGCTCGCCGAGCCGGGCAAGGAAGT
GCCACTCGTGGTGAATACCCCGAATCGTGCCAAGCCTCCTCGTCACTGGATCGATCTGAGCATCGAAGAAAGGGTGCAGG
CCGTCAAGGATTTCGGTGTGCCAGCTTTCCGTGCTCGGCAGATTTCCGCTCACGTCTTTGAGCGGTGGGAGGTCGACCCG
ACTCAGTGGACTGACTTGCCTAAGGCGGCCCGTCAGGAGATCGCCGACGCCTGGTTTCCGGTCCTGTTGACCAAGGTCTC
TCAGCAGTCGTGCGATCGCGGCACAACCGTCAAGACCCTGTGGCGGCTGCATGGTGGAGCTCTGGTTGAGTCGGTACTCA
TGTACTATCCAGCCACCCGTCACTCTGCGGCTCGCGCCACCTTGTGCCTGTCTTCGCAGGCCGGTTGCGGTATGGCCTGT
CCGTTTTGTGCCACCGGTCAGGGTGGTATTCAGCGCAATATGTCCACCGCCGAAATCGTCAGCCAGGTTTTGGCGGCGAA
TCGTCTCATAGCGGCAGGCGAGGTTCCTGGGGCGTCCGGACGCGTCCACAACATTGTCTTTATGGGTATGGGTGAGCCGA
TGGCCAATTACCGTTCGGTGCTTACTGCCATCCGGACCCTGACCGCCGACGGTCCCGACGGGGTCGGGATGAGCGCCCGG
GCCCTCACCCTCTCGACAGTCGGCCTGGTACCACGTATCAAGGCCTTGACGCAGGAGGGGATCCCGGTAACCCTCGCCGT
CTCATTACACGCCCCTGACGACGAGCTGCGTGATGAGCTCATCCCGGTTAACCGTCGTTGGAAAGTCGACGAGTTGCTCG
ACGCCGCCTGGCACTACGCCGAAAAGACGAAGCGTCGCGTCTCCATCGAGTACGCCCTCATGAAGGACATCAACGACCAG
GCCGACCGTGCCGCGGTGCTGGCCCGTCAGATCCGTCGTCGGGGCGATTGGACCTGGGCTCACGTCAACCTCATCCCTCT
CAATCCGACGCCCGGTTCCCGCTGGACGGCTTCGCGCCCCGAGGACCAGGATGCCTTCGTCGAGACACTTGAGCGCTGGA
AGATCCCGGTGACAGTGCGCGATACCCGAGGCTCTGAGATCGACGGTGCTTGTGGCCAGTTGGCAGCTGTGGGACGGTCG
GAAGGACTAGGCAGCTAA

Upstream 100 bases:

>100_bases
GCTAGTGCAGAACTGTTCGCCGAGGTGACGTCGCGACCCGTACTTAGCAGGCACCGACTCGTGTGGTCGGGTGGTGGGAG
TTGGGGATAATGGGGGCGTG

Downstream 100 bases:

>100_bases
CGGTTTGCGGCGGCCCCGGTGACAGTCCCGGTGTGCCTCGAGCGTCACAATCGTCCACACAGAAATACAGAAGGGATTCA
CCGTATGGATAGGCAACTCG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSSTGSSVSTSGLVLPSTPLAEPGKEVPLVVNTPNRAKPPRHWIDLSIEERVQAVKDFGVPAFRARQISAHVFERWEVDP
TQWTDLPKAARQEIADAWFPVLLTKVSQQSCDRGTTVKTLWRLHGGALVESVLMYYPATRHSAARATLCLSSQAGCGMAC
PFCATGQGGIQRNMSTAEIVSQVLAANRLIAAGEVPGASGRVHNIVFMGMGEPMANYRSVLTAIRTLTADGPDGVGMSAR
ALTLSTVGLVPRIKALTQEGIPVTLAVSLHAPDDELRDELIPVNRRWKVDELLDAAWHYAEKTKRRVSIEYALMKDINDQ
ADRAAVLARQIRRRGDWTWAHVNLIPLNPTPGSRWTASRPEDQDAFVETLERWKIPVTVRDTRGSEIDGACGQLAAVGRS
EGLGS

Sequences:

>Translated_405_residues
MSSTGSSVSTSGLVLPSTPLAEPGKEVPLVVNTPNRAKPPRHWIDLSIEERVQAVKDFGVPAFRARQISAHVFERWEVDP
TQWTDLPKAARQEIADAWFPVLLTKVSQQSCDRGTTVKTLWRLHGGALVESVLMYYPATRHSAARATLCLSSQAGCGMAC
PFCATGQGGIQRNMSTAEIVSQVLAANRLIAAGEVPGASGRVHNIVFMGMGEPMANYRSVLTAIRTLTADGPDGVGMSAR
ALTLSTVGLVPRIKALTQEGIPVTLAVSLHAPDDELRDELIPVNRRWKVDELLDAAWHYAEKTKRRVSIEYALMKDINDQ
ADRAAVLARQIRRRGDWTWAHVNLIPLNPTPGSRWTASRPEDQDAFVETLERWKIPVTVRDTRGSEIDGACGQLAAVGRS
EGLGS
>Mature_404_residues
SSTGSSVSTSGLVLPSTPLAEPGKEVPLVVNTPNRAKPPRHWIDLSIEERVQAVKDFGVPAFRARQISAHVFERWEVDPT
QWTDLPKAARQEIADAWFPVLLTKVSQQSCDRGTTVKTLWRLHGGALVESVLMYYPATRHSAARATLCLSSQAGCGMACP
FCATGQGGIQRNMSTAEIVSQVLAANRLIAAGEVPGASGRVHNIVFMGMGEPMANYRSVLTAIRTLTADGPDGVGMSARA
LTLSTVGLVPRIKALTQEGIPVTLAVSLHAPDDELRDELIPVNRRWKVDELLDAAWHYAEKTKRRVSIEYALMKDINDQA
DRAAVLARQIRRRGDWTWAHVNLIPLNPTPGSRWTASRPEDQDAFVETLERWKIPVTVRDTRGSEIDGACGQLAAVGRSE
GLGS

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=362, Percent_Identity=37.292817679558, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PROAC (Q6A7K4)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056219.1
- ProteinModelPortal:   Q6A7K4
- GeneID:   2933127
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1514
- NMPDR:   fig|267747.1.peg.1496
- HOGENOM:   HBG335345
- OMA:   DINDQPW
- ProtClustDB:   PRK14459
- BioCyc:   PACN267747:PPA1514-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 44124; Mature: 43993

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTGSSVSTSGLVLPSTPLAEPGKEVPLVVNTPNRAKPPRHWIDLSIEERVQAVKDFGV
CCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCEECCCHHHHHHHHHHCCC
PAFRARQISAHVFERWEVDPTQWTDLPKAARQEIADAWFPVLLTKVSQQSCDRGTTVKTL
CHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
WRLHGGALVESVLMYYPATRHSAARATLCLSSQAGCGMACPFCATGQGGIQRNMSTAEIV
HHHHCHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHH
SQVLAANRLIAAGEVPGASGRVHNIVFMGMGEPMANYRSVLTAIRTLTADGPDGVGMSAR
HHHHHHCCEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
ALTLSTVGLVPRIKALTQEGIPVTLAVSLHAPDDELRDELIPVNRRWKVDELLDAAWHYA
EEEHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHH
EKTKRRVSIEYALMKDINDQADRAAVLARQIRRRGDWTWAHVNLIPLNPTPGSRWTASRP
HHHHHHEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCC
EDQDAFVETLERWKIPVTVRDTRGSEIDGACGQLAAVGRSEGLGS
CCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSTGSSVSTSGLVLPSTPLAEPGKEVPLVVNTPNRAKPPRHWIDLSIEERVQAVKDFGV
CCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCEECCCHHHHHHHHHHCCC
PAFRARQISAHVFERWEVDPTQWTDLPKAARQEIADAWFPVLLTKVSQQSCDRGTTVKTL
CHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
WRLHGGALVESVLMYYPATRHSAARATLCLSSQAGCGMACPFCATGQGGIQRNMSTAEIV
HHHHCHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHH
SQVLAANRLIAAGEVPGASGRVHNIVFMGMGEPMANYRSVLTAIRTLTADGPDGVGMSAR
HHHHHHCCEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
ALTLSTVGLVPRIKALTQEGIPVTLAVSLHAPDDELRDELIPVNRRWKVDELLDAAWHYA
EEEHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHH
EKTKRRVSIEYALMKDINDQADRAAVLARQIRRRGDWTWAHVNLIPLNPTPGSRWTASRP
HHHHHHEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCC
EDQDAFVETLERWKIPVTVRDTRGSEIDGACGQLAAVGRSEGLGS
CCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA