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The map label for this gene is srlE [H]

Identifier: 50842963

GI number: 50842963

Start: 1602846

End: 1603895

Strand: Direct

Name: srlE [H]

Synonym: PPA1485

Alternate gene names: 50842963

Gene position: 1602846-1603895 (Clockwise)

Preceding gene: 50842962

Following gene: 50842964

Centisome position: 62.6

GC content: 61.62

Gene sequence:

>1050_bases
ATGACCGAGTACCGCTCCGTCACCGTTTCCCATGGCTCCGGCGGCTACGGCGGCCCCCTCACCATCACCCCTACCGAGGC
TAAACACAAGCTCGTCTACATCGTCGGCGGAGGCGTCCGGCCCCCGGTCGTTGATCGCATCGCCGAGCTGTCTGGTGCCG
AACCGGTCAATGGGTTTAACACCTCCATCCCCGACGAGGAGATGATGGCCGTCATCATCGACTGCGGGGGGACCCTAAGG
TGCGGAATCTACCCTCGCAAGGGAATCCCGACTATCAACGTCATGCCAACCGGTAAGAGCGGTCCGCTGGCCCAATACAT
CAACGAGTCGATCTACGTCTCCGCCGTCGACGTCGACCAGGTCCGGTTGGCCGACTCACCTACATCCTCCGCCACTGTCA
CCTCCCCCGCTATAGCCGCCGCTGCCGCGCCACGGTCAAAAGCCGGAACAGCAGGCACCGTCCAGGGGGAATGGAGCACT
GACAAGAAGATTTCCGAAGTCGTCGCCGAGAAGAAGAACAAGACCGTCATGGACAAGATCGGCCTGGGCGCCGGCAAGGT
CATCTCGGTGTTCTACCAAGCCGCTCGAGACGCGGTGCAGGCCATGCTGACCACCATCATCCCCTTCATGGGGTTCGTAG
CCATGCTTATCGGCATCATCAACGCCTCCGGATTCGGTACCTGGTTCACCAAACTTCTCATCCCGCTAGCCGGCAACGGC
ATCGGGCTCATCGTCCTTGGCCTCATCTGCTCGCTGCCCTTCCTGTCGCCCCTGCTCGGCCCAGGAGCCGTCATCGGTCA
GATCATCGGAACTCTCATCGGCGTAGAGATCGGCAAGCACAACATCCATCCCAGCTTGGCCCTTCCAGCGCTGTTCGCCA
TTAACACCCAGAACGCCTGCGACTTCATCCCGGTCGGCCTAGGACTAGCTGAGGCTGCCCCGGAAACAGTCGAGGTTGGC
GTCCCCAGCGTCTTGTACTCGCGATTCCTTAACGGCGTCCCACGAGTCGCCATCGCCTGGCTGTGCAGCATCGGCCTGTA
CGCATCCTGA

Upstream 100 bases:

>100_bases
GACTTAACTACCAGATACGTGAGCAGGCAGCAGAAGGTCATCCTCCCCGACACCATGGACGGGAAGATCGTCCACGTCGA
CACCCTTGTGGAGGCCTGAG

Downstream 100 bases:

>100_bases
CACTCAAACCTGATCCGGAGAACATCAATGTCTGTCATCTATCGCAATCAGATCTGCGCCGTCGGCTCGCAGGCCGAGTC
CTTTCTCAGCGAGAAAATGC

Product: PTS system, glucitol/sorbitol-specific IIBC component

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTEYRSVTVSHGSGGYGGPLTITPTEAKHKLVYIVGGGVRPPVVDRIAELSGAEPVNGFNTSIPDEEMMAVIIDCGGTLR
CGIYPRKGIPTINVMPTGKSGPLAQYINESIYVSAVDVDQVRLADSPTSSATVTSPAIAAAAAPRSKAGTAGTVQGEWST
DKKISEVVAEKKNKTVMDKIGLGAGKVISVFYQAARDAVQAMLTTIIPFMGFVAMLIGIINASGFGTWFTKLLIPLAGNG
IGLIVLGLICSLPFLSPLLGPGAVIGQIIGTLIGVEIGKHNIHPSLALPALFAINTQNACDFIPVGLGLAEAAPETVEVG
VPSVLYSRFLNGVPRVAIAWLCSIGLYAS

Sequences:

>Translated_349_residues
MTEYRSVTVSHGSGGYGGPLTITPTEAKHKLVYIVGGGVRPPVVDRIAELSGAEPVNGFNTSIPDEEMMAVIIDCGGTLR
CGIYPRKGIPTINVMPTGKSGPLAQYINESIYVSAVDVDQVRLADSPTSSATVTSPAIAAAAAPRSKAGTAGTVQGEWST
DKKISEVVAEKKNKTVMDKIGLGAGKVISVFYQAARDAVQAMLTTIIPFMGFVAMLIGIINASGFGTWFTKLLIPLAGNG
IGLIVLGLICSLPFLSPLLGPGAVIGQIIGTLIGVEIGKHNIHPSLALPALFAINTQNACDFIPVGLGLAEAAPETVEVG
VPSVLYSRFLNGVPRVAIAWLCSIGLYAS
>Mature_348_residues
TEYRSVTVSHGSGGYGGPLTITPTEAKHKLVYIVGGGVRPPVVDRIAELSGAEPVNGFNTSIPDEEMMAVIIDCGGTLRC
GIYPRKGIPTINVMPTGKSGPLAQYINESIYVSAVDVDQVRLADSPTSSATVTSPAIAAAAAPRSKAGTAGTVQGEWSTD
KKISEVVAEKKNKTVMDKIGLGAGKVISVFYQAARDAVQAMLTTIIPFMGFVAMLIGIINASGFGTWFTKLLIPLAGNGI
GLIVLGLICSLPFLSPLLGPGAVIGQIIGTLIGVEIGKHNIHPSLALPALFAINTQNACDFIPVGLGLAEAAPETVEVGV
PSVLYSRFLNGVPRVAIAWLCSIGLYAS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=348, Percent_Identity=50.5747126436782, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 36143; Mature: 36011

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYRSVTVSHGSGGYGGPLTITPTEAKHKLVYIVGGGVRPPVVDRIAELSGAEPVNGFN
CCCCEEEEEECCCCCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCC
TSIPDEEMMAVIIDCGGTLRCGIYPRKGIPTINVMPTGKSGPLAQYINESIYVSAVDVDQ
CCCCCHHHEEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCHHHHCCCCEEEEEECCCC
VRLADSPTSSATVTSPAIAAAAAPRSKAGTAGTVQGEWSTDKKISEVVAEKKNKTVMDKI
EEECCCCCCCCEECCCHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCHHHHHH
GLGAGKVISVFYQAARDAVQAMLTTIIPFMGFVAMLIGIINASGFGTWFTKLLIPLAGNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
IGLIVLGLICSLPFLSPLLGPGAVIGQIIGTLIGVEIGKHNIHPSLALPALFAINTQNAC
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCEEEEECCCCCC
DFIPVGLGLAEAAPETVEVGVPSVLYSRFLNGVPRVAIAWLCSIGLYAS
CEEEECCCHHHCCCCCEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TEYRSVTVSHGSGGYGGPLTITPTEAKHKLVYIVGGGVRPPVVDRIAELSGAEPVNGFN
CCCEEEEEECCCCCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCC
TSIPDEEMMAVIIDCGGTLRCGIYPRKGIPTINVMPTGKSGPLAQYINESIYVSAVDVDQ
CCCCCHHHEEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCHHHHCCCCEEEEEECCCC
VRLADSPTSSATVTSPAIAAAAAPRSKAGTAGTVQGEWSTDKKISEVVAEKKNKTVMDKI
EEECCCCCCCCEECCCHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCHHHHHH
GLGAGKVISVFYQAARDAVQAMLTTIIPFMGFVAMLIGIINASGFGTWFTKLLIPLAGNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
IGLIVLGLICSLPFLSPLLGPGAVIGQIIGTLIGVEIGKHNIHPSLALPALFAINTQNAC
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCEEEEECCCCCC
DFIPVGLGLAEAAPETVEVGVPSVLYSRFLNGVPRVAIAWLCSIGLYAS
CEEEECCCHHHCCCCCEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9572925 [H]