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The map label for this gene is truB

Identifier: 50842956

GI number: 50842956

Start: 1597386

End: 1598273

Strand: Reverse

Name: truB

Synonym: PPA1478

Alternate gene names: 50842956

Gene position: 1598273-1597386 (Counterclockwise)

Preceding gene: 50842957

Following gene: 50842955

Centisome position: 62.43

GC content: 61.26

Gene sequence:

>888_bases
ATGAATACTCTCCAGTCTGGCGTCCTCGTCGTTGACAAACCTGCCGGCGTCACGTCTCACCAGGTGGTTGGGCGGGTGCG
GCGCCTCATGGGTACTCGCAAGGTCGGCCACGCTGGAACTCTTGACCCGATGGCCAGCGGCGTCCTCGTCGTTGGGGTCA
ATCGCGCGACTCGCCTGTTGGGTCACTTGTCTCTGCATGATAAGGACTACACCGCCACCGTGAGGCTAGGGGTGGGCACT
GTCACCGATGACGCCGAGGGGGACGTCACTGTGACCACCGACGCTTCCGCAATCGACGACAGGGCCATTCACGCCGCTAT
GGTGCGTCAGACAGGAGAGATCCAACAGGTTCCCGCTGCCGTCAGCGCGATCAAGGTTAACGGCCGTCGGGCCTACGCCA
AGGTGCGTGCTGGTGAGGACGTCGTCCTACGGCCGCGCGCTGTCACCGTTTCTCGGTTTGAGGCTATTGCTATTCGACGT
CACGGTCAGGTAATTGACGTCGACGTCGAGGCTACGTGCTCCTCGGGAACCTACGTGCGGGCGCTGGCTCGTGACGTCGG
TGCGGATCTTGGAGTCGGGGGACATCTCACCGCCCTGCGTCGTACTCGCGTCGGCCCGTTCGATCTCACCGCTGCCTGTG
TAGATATTTTCGCGCAGGACGCCGTCACACCCACCCCGATGACAATGGCTGAGGCCGCTGCACTCAGTTTCCCCGTTGTT
CACGTCACTGCGGACCAAGCAGCTGCTATCCGGGTGGGTCGACGTCTAAGTTTCACGGTGCCGGCGGAAGTGACCGCAAT
AATTGCGGAAACTGGTGAATTGTTGGCCCTATATCGTCCGGATGACGAGAAAGATGGCCAGAGTCGCGCAATATGTGTGC
TGGTGTGA

Upstream 100 bases:

>100_bases
AGGGCAGTTGAGGCCTCGAAAATGGCCCTGTACATGATCACCGCGTGACTTTGGGCAGACCAGCTTGTTGAAGCCCTCCT
GAGTCGGGATACTGGCTCCG

Downstream 100 bases:

>100_bases
CGACGCCGTCACACCACTTTGGATAGAGCGCGCCATGATACGCTCAACTGCATAGTCATGGTCGAAGTAATCTTTGGCGC
GCCGGGCGATATTACTCACA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNTLQSGVLVVDKPAGVTSHQVVGRVRRLMGTRKVGHAGTLDPMASGVLVVGVNRATRLLGHLSLHDKDYTATVRLGVGT
VTDDAEGDVTVTTDASAIDDRAIHAAMVRQTGEIQQVPAAVSAIKVNGRRAYAKVRAGEDVVLRPRAVTVSRFEAIAIRR
HGQVIDVDVEATCSSGTYVRALARDVGADLGVGGHLTALRRTRVGPFDLTAACVDIFAQDAVTPTPMTMAEAAALSFPVV
HVTADQAAAIRVGRRLSFTVPAEVTAIIAETGELLALYRPDDEKDGQSRAICVLV

Sequences:

>Translated_295_residues
MNTLQSGVLVVDKPAGVTSHQVVGRVRRLMGTRKVGHAGTLDPMASGVLVVGVNRATRLLGHLSLHDKDYTATVRLGVGT
VTDDAEGDVTVTTDASAIDDRAIHAAMVRQTGEIQQVPAAVSAIKVNGRRAYAKVRAGEDVVLRPRAVTVSRFEAIAIRR
HGQVIDVDVEATCSSGTYVRALARDVGADLGVGGHLTALRRTRVGPFDLTAACVDIFAQDAVTPTPMTMAEAAALSFPVV
HVTADQAAAIRVGRRLSFTVPAEVTAIIAETGELLALYRPDDEKDGQSRAICVLV
>Mature_295_residues
MNTLQSGVLVVDKPAGVTSHQVVGRVRRLMGTRKVGHAGTLDPMASGVLVVGVNRATRLLGHLSLHDKDYTATVRLGVGT
VTDDAEGDVTVTTDASAIDDRAIHAAMVRQTGEIQQVPAAVSAIKVNGRRAYAKVRAGEDVVLRPRAVTVSRFEAIAIRR
HGQVIDVDVEATCSSGTYVRALARDVGADLGVGGHLTALRRTRVGPFDLTAACVDIFAQDAVTPTPMTMAEAAALSFPVV
HVTADQAAAIRVGRRLSFTVPAEVTAIIAETGELLALYRPDDEKDGQSRAICVLV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=222, Percent_Identity=36.036036036036, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI4503337, Length=205, Percent_Identity=32.1951219512195, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI215599015, Length=205, Percent_Identity=32.1951219512195, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI2367200, Length=295, Percent_Identity=31.1864406779661, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553978, Length=207, Percent_Identity=31.4009661835749, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=202, Percent_Identity=33.1683168316832, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=185, Percent_Identity=25.4054054054054, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364189, Length=202, Percent_Identity=29.7029702970297, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364187, Length=202, Percent_Identity=29.7029702970297, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364185, Length=202, Percent_Identity=29.7029702970297, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364183, Length=202, Percent_Identity=29.7029702970297, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI62471759, Length=202, Percent_Identity=29.7029702970297, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17975520, Length=202, Percent_Identity=29.7029702970297, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PROAC (Q6A7P0)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056183.1
- ProteinModelPortal:   Q6A7P0
- SMR:   Q6A7P0
- GeneID:   2932302
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1478
- NMPDR:   fig|267747.1.peg.1460
- HOGENOM:   HBG397258
- OMA:   ERTISEP
- ProtClustDB:   PRK03287
- BioCyc:   PACN267747:PPA1478-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 31072; Mature: 31072

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: ACT_SITE 42-42

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLQSGVLVVDKPAGVTSHQVVGRVRRLMGTRKVGHAGTLDPMASGVLVVGVNRATRLL
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCHHHHHH
GHLSLHDKDYTATVRLGVGTVTDDAEGDVTVTTDASAIDDRAIHAAMVRQTGEIQQVPAA
HHHEECCCCEEEEEEEECEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHCCE
VSAIKVNGRRAYAKVRAGEDVVLRPRAVTVSRFEAIAIRRHGQVIDVDVEATCSSGTYVR
EEEEEECCCEEEEEEECCCCEEECCCEEEHHHHEEEEEECCCCEEEEEEEEECCCCHHHH
ALARDVGADLGVGGHLTALRRTRVGPFDLTAACVDIFAQDAVTPTPMTMAEAAALSFPVV
HHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEE
HVTADQAAAIRVGRRLSFTVPAEVTAIIAETGELLALYRPDDEKDGQSRAICVLV
EEECCCHHHHHHCCEEEEECCHHHEEEEECCCCEEEEECCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MNTLQSGVLVVDKPAGVTSHQVVGRVRRLMGTRKVGHAGTLDPMASGVLVVGVNRATRLL
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCHHHHHH
GHLSLHDKDYTATVRLGVGTVTDDAEGDVTVTTDASAIDDRAIHAAMVRQTGEIQQVPAA
HHHEECCCCEEEEEEEECEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHCCE
VSAIKVNGRRAYAKVRAGEDVVLRPRAVTVSRFEAIAIRRHGQVIDVDVEATCSSGTYVR
EEEEEECCCEEEEEEECCCCEEECCCEEEHHHHEEEEEECCCCEEEEEEEEECCCCHHHH
ALARDVGADLGVGGHLTALRRTRVGPFDLTAACVDIFAQDAVTPTPMTMAEAAALSFPVV
HHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEE
HVTADQAAAIRVGRRLSFTVPAEVTAIIAETGELLALYRPDDEKDGQSRAICVLV
EEECCCHHHHHHCCEEEEECCHHHEEEEECCCCEEEEECCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA