Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 50842954

GI number: 50842954

Start: 1594875

End: 1595741

Strand: Reverse

Name: opuAB [H]

Synonym: PPA1476

Alternate gene names: 50842954

Gene position: 1595741-1594875 (Counterclockwise)

Preceding gene: 50842955

Following gene: 50842953

Centisome position: 62.33

GC content: 57.9

Gene sequence:

>867_bases
ATGGGCTTCAATTTCGCTAACCCTCGAATTCCGCTCGGTAGCTGGATCGAGACCTTCTTTGACTGGTTCCAGAACGCCTT
TGACTGGCTCATCACGTTCACAAACGTTGTCATCGGTGGCATGTACCACGGTCTCAATCTGGTGCTGTCCAAACCGTTAT
ACCTAGTCATGATGGTGGTCTTTGCCGCTATCGGTTGGGCCTGCGCCAACTGGAGAGTGGGCCTGTTTGCCCTCATCGGT
TTCTATCTGGTGAGATCCATGGACCAGTGGAACAACACCATGCAAACCCTCGCTTTAGTGGTGGTTGCGGTCATCCTGGC
CCTTATCCTGGCAATCCCATTGGGTATCTGGGCGGCCAAGTCGAAGCGATTCTCGGCCATCATCAAACCGATCCTCGACT
TTATGCAAACGATGCCTTCTTTGGCATACCTGGTTCCGGTCATCGTGCTCTTCGGTATTGGACAGGTGCCCGGTGCCATC
GCGACCATCATCTTCGCGCTGCCGCCGGGAGTGCGGTTCACCGAGCTGGGCATCCGTGGAGTCGACACTGAGACCGTTGA
GGCCGGCGCCGCATTCGGTGCTAGCCCCCGTCACATCCTGTGGCGTATTCAGATGCCGCTAGCTATGCCGACGGTGATGG
CCGGTGTTAACCAGGTCATCATGCTGGCCCTGTCCATGGTTGTGCTCTCGGGCATGGTCGGTGCTGGTGGCCTCGGCGGT
GACGTCGTCGCAGCCCTGTCTCAAGTTGATATTGGTCTAGGCATGGAGGCTGGCTTGGCGGTGGTTATCGTCGCTGTATA
CCTAGACCGCATCACCAACGGACTAGCCAATCGCACTCCGCTCGCCCGGGCGCAGCGGGCGAGCTGA

Upstream 100 bases:

>100_bases
AGAACACCGGAAAGCTTCCCATTATCAGGGATACCGGTAACCCGGTCGGTCTTCCGCCGGCTGACCAAATTGCGACGATC
GAGAAGGAGGCCTGATCGCC

Downstream 100 bases:

>100_bases
GTCAATGCACATGAACACGAGGAAGTACCGATGAGCAGAAGCATGGTTCAACGCAAGGTCATCGCCGTGGCATCCGCCCT
GACGGTCGGGGCCCTGGCTC

Product: glycine betaine transport system permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MGFNFANPRIPLGSWIETFFDWFQNAFDWLITFTNVVIGGMYHGLNLVLSKPLYLVMMVVFAAIGWACANWRVGLFALIG
FYLVRSMDQWNNTMQTLALVVVAVILALILAIPLGIWAAKSKRFSAIIKPILDFMQTMPSLAYLVPVIVLFGIGQVPGAI
ATIIFALPPGVRFTELGIRGVDTETVEAGAAFGASPRHILWRIQMPLAMPTVMAGVNQVIMLALSMVVLSGMVGAGGLGG
DVVAALSQVDIGLGMEAGLAVVIVAVYLDRITNGLANRTPLARAQRAS

Sequences:

>Translated_288_residues
MGFNFANPRIPLGSWIETFFDWFQNAFDWLITFTNVVIGGMYHGLNLVLSKPLYLVMMVVFAAIGWACANWRVGLFALIG
FYLVRSMDQWNNTMQTLALVVVAVILALILAIPLGIWAAKSKRFSAIIKPILDFMQTMPSLAYLVPVIVLFGIGQVPGAI
ATIIFALPPGVRFTELGIRGVDTETVEAGAAFGASPRHILWRIQMPLAMPTVMAGVNQVIMLALSMVVLSGMVGAGGLGG
DVVAALSQVDIGLGMEAGLAVVIVAVYLDRITNGLANRTPLARAQRAS
>Mature_287_residues
GFNFANPRIPLGSWIETFFDWFQNAFDWLITFTNVVIGGMYHGLNLVLSKPLYLVMMVVFAAIGWACANWRVGLFALIGF
YLVRSMDQWNNTMQTLALVVVAVILALILAIPLGIWAAKSKRFSAIIKPILDFMQTMPSLAYLVPVIVLFGIGQVPGAIA
TIIFALPPGVRFTELGIRGVDTETVEAGAAFGASPRHILWRIQMPLAMPTVMAGVNQVIMLALSMVVLSGMVGAGGLGGD
VVAALSQVDIGLGMEAGLAVVIVAVYLDRITNGLANRTPLARAQRAS

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=288, Percent_Identity=42.7083333333333, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=29.7435897435897, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788449, Length=236, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786564, Length=157, Percent_Identity=26.7515923566879, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30952; Mature: 30820

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFNFANPRIPLGSWIETFFDWFQNAFDWLITFTNVVIGGMYHGLNLVLSKPLYLVMMVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAAIGWACANWRVGLFALIGFYLVRSMDQWNNTMQTLALVVVAVILALILAIPLGIWAAK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKRFSAIIKPILDFMQTMPSLAYLVPVIVLFGIGQVPGAIATIIFALPPGVRFTELGIRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHCCCCC
VDTETVEAGAAFGASPRHILWRIQMPLAMPTVMAGVNQVIMLALSMVVLSGMVGAGGLGG
CCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DVVAALSQVDIGLGMEAGLAVVIVAVYLDRITNGLANRTPLARAQRAS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
GFNFANPRIPLGSWIETFFDWFQNAFDWLITFTNVVIGGMYHGLNLVLSKPLYLVMMVV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAAIGWACANWRVGLFALIGFYLVRSMDQWNNTMQTLALVVVAVILALILAIPLGIWAAK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKRFSAIIKPILDFMQTMPSLAYLVPVIVLFGIGQVPGAIATIIFALPPGVRFTELGIRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHCCCCC
VDTETVEAGAAFGASPRHILWRIQMPLAMPTVMAGVNQVIMLALSMVVLSGMVGAGGLGG
CCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DVVAALSQVDIGLGMEAGLAVVIVAVYLDRITNGLANRTPLARAQRAS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]