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The map label for this gene is ogt [H]

Identifier: 50842908

GI number: 50842908

Start: 1547186

End: 1547713

Strand: Direct

Name: ogt [H]

Synonym: PPA1428

Alternate gene names: 50842908

Gene position: 1547186-1547713 (Clockwise)

Preceding gene: 50842907

Following gene: 50842928

Centisome position: 60.43

GC content: 58.71

Gene sequence:

>528_bases
GTGCCCGGCCCGCTTCCTGCTATGACAGCACAGTCCCAGCACCGCTTTGACACCGCCATTGGCGAGTTAGAAGTAACAGC
TACCGCTCATGGGCTCAGTCGGCTCGTCTTCGTCAAGACAGATCCATCACCGCCGCCACTTTCGACATCACATGCTCGCC
GCAGCGGTGACATCATCTCTTGTGCCATTGCACAGATCACCGAGTACTTGTCAGGATCGCGGACCTCATTCGACGTAGCG
CTGGATCTTTCTGCGACGACGGACTTCCAACGCACTGTACTAACCGGGTTGCAAACAGTGCCTTATGGGCAAACTGTCAG
CTACCGCCAACTTGCGAGCATCATTGGACGGCCGAATGCGTCACGCGCCGTCGGCCACGCCTGTGCAACAAACCCTCTGC
CCATCCTCATTCCGTGCCACCGCGTGCTACGCAGTAACGGCCAGCTAGGAGGCTATCTCGGTGGTCCTCGCCTCAAGCGC
TTCCTGCTCAATCTAGAGAGTGTCACCTCCGCTCCCTTGCCAGCGTGA

Upstream 100 bases:

>100_bases
GAAGGTCGAGGACGTCAAAGACAAGGCGAATGACCTCAAGCACAATGTCCAGGCTGCGGCTGACAAGCTAAAGGGTTGAT
AATGTCGGGCCGGGCATCAG

Downstream 100 bases:

>100_bases
CCGACCAGGTGCCGTCGAGGCAACAAGCCGCCATGCCGAGTTGTTCGAGGACGCTTAGCGCAGCCGAACACTGAGCAACG
GGAACTCCAGCCAAAACCGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MPGPLPAMTAQSQHRFDTAIGELEVTATAHGLSRLVFVKTDPSPPPLSTSHARRSGDIISCAIAQITEYLSGSRTSFDVA
LDLSATTDFQRTVLTGLQTVPYGQTVSYRQLASIIGRPNASRAVGHACATNPLPILIPCHRVLRSNGQLGGYLGGPRLKR
FLLNLESVTSAPLPA

Sequences:

>Translated_175_residues
MPGPLPAMTAQSQHRFDTAIGELEVTATAHGLSRLVFVKTDPSPPPLSTSHARRSGDIISCAIAQITEYLSGSRTSFDVA
LDLSATTDFQRTVLTGLQTVPYGQTVSYRQLASIIGRPNASRAVGHACATNPLPILIPCHRVLRSNGQLGGYLGGPRLKR
FLLNLESVTSAPLPA
>Mature_174_residues
PGPLPAMTAQSQHRFDTAIGELEVTATAHGLSRLVFVKTDPSPPPLSTSHARRSGDIISCAIAQITEYLSGSRTSFDVAL
DLSATTDFQRTVLTGLQTVPYGQTVSYRQLASIIGRPNASRAVGHACATNPLPILIPCHRVLRSNGQLGGYLGGPRLKRF
LLNLESVTSAPLPA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=79, Percent_Identity=51.8987341772152, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1788542, Length=105, Percent_Identity=45.7142857142857, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=43.6893203883495, Blast_Score=92, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=47.5, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=47.5, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=47.1264367816092, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18588; Mature: 18456

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGPLPAMTAQSQHRFDTAIGELEVTATAHGLSRLVFVKTDPSPPPLSTSHARRSGDIIS
CCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHCCCCHHH
CAIAQITEYLSGSRTSFDVALDLSATTDFQRTVLTGLQTVPYGQTVSYRQLASIIGRPNA
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCC
SRAVGHACATNPLPILIPCHRVLRSNGQLGGYLGGPRLKRFLLNLESVTSAPLPA
HHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PGPLPAMTAQSQHRFDTAIGELEVTATAHGLSRLVFVKTDPSPPPLSTSHARRSGDIIS
CCCCCCCCCCCHHHHHHHHCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHCCCCHHH
CAIAQITEYLSGSRTSFDVALDLSATTDFQRTVLTGLQTVPYGQTVSYRQLASIIGRPNA
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCC
SRAVGHACATNPLPILIPCHRVLRSNGQLGGYLGGPRLKRFLLNLESVTSAPLPA
HHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]