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The map label for this gene is gsiB [H]

Identifier: 50842756

GI number: 50842756

Start: 1383984

End: 1385471

Strand: Reverse

Name: gsiB [H]

Synonym: PPA1277

Alternate gene names: 50842756

Gene position: 1385471-1383984 (Counterclockwise)

Preceding gene: 50842759

Following gene: 50842755

Centisome position: 54.11

GC content: 59.01

Gene sequence:

>1488_bases
ATGCGAGACCAGCTCAAAGCTGCAGGGCTAGCCGTTGCGGTAGCTTTGTCGGCCACAGGAATCGCCGGTTGCCAGTCGAC
TGACGACAACGTACTCACCATCGGGGCAACAGCAGCCCCTCCAACCCTCGACTTAGTGTCCAACGCGGCCGCGGCGATTC
CGCAGGTCCTGCTCTATAACGTTTATGAGACTCTCGTTCGGGTTAATGATTCGGGCAAGCTCGTCGGGTTGCTGGCGAAG
TCGTGGAAGCTTTCTGATGACGGTCTGAGACTCACTTTTCGCCTTGACCCCAACGCCCGGTTCGCGTCTGGCGCCCGGGT
GACTTCGGCTGCCGTCAAGGCTTCCCTAGAGCGTATGAGGGGTGCGTCGGCAAGCCCGGTGAATCAGGCAAACCTGGCTG
CGATCAAGGCTATTCATACCCCGGATGACTCCACCGTCATCCTCGATCTGTCGAATCGCGACAACTTCCTATTGTTTAAC
CTGGCCAGCACGTCGGGAGTTGTCATCGATCCGGCGACGAAGGACCTGGCTACCCGACCTCAAGGGTCCGGACCCTATGT
CGTCACTGAGTTCACCCCAGGACATTCGGTGACCTTGTCTCCGTCTCCGCGACCTTGGCACGAGGGGAATCGCCCTACCG
TCCGCTTTGCTTACTATTCCGACCCGACATCTCAGACGGCAGCCCTGCTGTCGGGGGATCTCGACGTGGTCTCCGACATG
ACGACTCCGCAAGCGTTATCCCGGTTTACCGGTAACCCGAACTATCGGGTGTTACGTGGGACCACCAACGGCGAGGTGGT
CCTCGGAATGAACAATACGTCGAAGGCTCTCTCAGACCGGCGGGTGCGTCAGGCCATCCTTATGGCGATTGACCGGAAGG
GTCTGCTTGACGTCGTCTGGAACGGGCAGGGAACTCTCATCGGGTCGATGGTTCCTCCCACTGACCCCTGGTATACCGAC
CTGTCTCATACCTGGCCACATGATCCGGTCCGGGCACGAAAATTGCTTGCTGACGCCGGGTATGGCAACGGCCTTACTCT
CAGGCTCCGTACAGCCGCATTGCCCTACGCGACTGCAGCGTCCAGGGTGGTGGCCTCTGAGCTTGCCCAGGTTGGCATCA
ACGTCGTCACCGACGAGCTGGAATTTCCCGCCCGTTGGCTCGATGTCGTTTACACCCATGCGGACTACGACCTCACGATC
GTCGCCCATGTCGAGGCTCGCGACATTGTCAACTGGGCGAACCCGGATTACTACTGGCGTTACCACAATAAGGAATTTAA
TCGTCTTATCGAGTCGGCGCGCACTGGTCCGGCCGATCGCACCAACGAGACGATGATGCAGGCCAGCCGGATCCTGGCTA
CCGACGCCGCGGGTGGGTTTCTCTTCCTTATGCCGAAGATCACCATCAGCAAGTCTGGCATCACCGGTCTGCAACGCAAC
TCAACGTCAATGAGTTTCGATCTGACCAAGGTGAGGAGGTCGAAGTGA

Upstream 100 bases:

>100_bases
GGGCGCCCTACCACACTGATCTCGAGGGGTTCGCGTAGGGCGCACAGGGGGTCTATGCTCGCCGGCAGGGCGCCGAGCCC
GAAGGGAGCATGACTAGACG

Downstream 100 bases:

>100_bases
CCGTCCTCAAACGTGTCTTAATTCCCTTAGGGGAGCTGCTGGTGTGGCTAATCGTCGCTTCCTTTGTCGTTTTTGCGCTA
CTCAGCTTGCTACCCGGGGA

Product: ABC transporter, substrate binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRDQLKAAGLAVAVALSATGIAGCQSTDDNVLTIGATAAPPTLDLVSNAAAAIPQVLLYNVYETLVRVNDSGKLVGLLAK
SWKLSDDGLRLTFRLDPNARFASGARVTSAAVKASLERMRGASASPVNQANLAAIKAIHTPDDSTVILDLSNRDNFLLFN
LASTSGVVIDPATKDLATRPQGSGPYVVTEFTPGHSVTLSPSPRPWHEGNRPTVRFAYYSDPTSQTAALLSGDLDVVSDM
TTPQALSRFTGNPNYRVLRGTTNGEVVLGMNNTSKALSDRRVRQAILMAIDRKGLLDVVWNGQGTLIGSMVPPTDPWYTD
LSHTWPHDPVRARKLLADAGYGNGLTLRLRTAALPYATAASRVVASELAQVGINVVTDELEFPARWLDVVYTHADYDLTI
VAHVEARDIVNWANPDYYWRYHNKEFNRLIESARTGPADRTNETMMQASRILATDAAGGFLFLMPKITISKSGITGLQRN
STSMSFDLTKVRRSK

Sequences:

>Translated_495_residues
MRDQLKAAGLAVAVALSATGIAGCQSTDDNVLTIGATAAPPTLDLVSNAAAAIPQVLLYNVYETLVRVNDSGKLVGLLAK
SWKLSDDGLRLTFRLDPNARFASGARVTSAAVKASLERMRGASASPVNQANLAAIKAIHTPDDSTVILDLSNRDNFLLFN
LASTSGVVIDPATKDLATRPQGSGPYVVTEFTPGHSVTLSPSPRPWHEGNRPTVRFAYYSDPTSQTAALLSGDLDVVSDM
TTPQALSRFTGNPNYRVLRGTTNGEVVLGMNNTSKALSDRRVRQAILMAIDRKGLLDVVWNGQGTLIGSMVPPTDPWYTD
LSHTWPHDPVRARKLLADAGYGNGLTLRLRTAALPYATAASRVVASELAQVGINVVTDELEFPARWLDVVYTHADYDLTI
VAHVEARDIVNWANPDYYWRYHNKEFNRLIESARTGPADRTNETMMQASRILATDAAGGFLFLMPKITISKSGITGLQRN
STSMSFDLTKVRRSK
>Mature_495_residues
MRDQLKAAGLAVAVALSATGIAGCQSTDDNVLTIGATAAPPTLDLVSNAAAAIPQVLLYNVYETLVRVNDSGKLVGLLAK
SWKLSDDGLRLTFRLDPNARFASGARVTSAAVKASLERMRGASASPVNQANLAAIKAIHTPDDSTVILDLSNRDNFLLFN
LASTSGVVIDPATKDLATRPQGSGPYVVTEFTPGHSVTLSPSPRPWHEGNRPTVRFAYYSDPTSQTAALLSGDLDVVSDM
TTPQALSRFTGNPNYRVLRGTTNGEVVLGMNNTSKALSDRRVRQAILMAIDRKGLLDVVWNGQGTLIGSMVPPTDPWYTD
LSHTWPHDPVRARKLLADAGYGNGLTLRLRTAALPYATAASRVVASELAQVGINVVTDELEFPARWLDVVYTHADYDLTI
VAHVEARDIVNWANPDYYWRYHNKEFNRLIESARTGPADRTNETMMQASRILATDAAGGFLFLMPKITISKSGITGLQRN
STSMSFDLTKVRRSK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=380, Percent_Identity=28.1578947368421, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787762, Length=481, Percent_Identity=27.8586278586279, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789887, Length=348, Percent_Identity=24.4252873563218, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789966, Length=421, Percent_Identity=22.8028503562945, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787551, Length=404, Percent_Identity=22.5247524752475, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082063, Length=267, Percent_Identity=26.9662921348315, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 53539; Mature: 53539

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDQLKAAGLAVAVALSATGIAGCQSTDDNVLTIGATAAPPTLDLVSNAAAAIPQVLLYN
CCCCHHHCCEEEEEEEHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VYETLVRVNDSGKLVGLLAKSWKLSDDGLRLTFRLDPNARFASGARVTSAAVKASLERMR
HHHHHEEECCCCCEEEEEECCCEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHC
GASASPVNQANLAAIKAIHTPDDSTVILDLSNRDNFLLFNLASTSGVVIDPATKDLATRP
CCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCEEECCCCHHHHCCC
QGSGPYVVTEFTPGHSVTLSPSPRPWHEGNRPTVRFAYYSDPTSQTAALLSGDLDVVSDM
CCCCCEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCHHHHHCC
TTPQALSRFTGNPNYRVLRGTTNGEVVLGMNNTSKALSDRRVRQAILMAIDRKGLLDVVW
CCHHHHHHHCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
NGQGTLIGSMVPPTDPWYTDLSHTWPHDPVRARKLLADAGYGNGLTLRLRTAALPYATAA
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHH
SRVVASELAQVGINVVTDELEFPARWLDVVYTHADYDLTIVAHVEARDIVNWANPDYYWR
HHHHHHHHHHCCCEEEECCCCCCHHHEEEEEEECCCEEEEEEEECHHHHHCCCCCCEEEE
YHNKEFNRLIESARTGPADRTNETMMQASRILATDAAGGFLFLMPKITISKSGITGLQRN
ECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCCCCEEEEEEEEEEECCCCCCCCCC
STSMSFDLTKVRRSK
CCCEEEEHHHHHCCC
>Mature Secondary Structure
MRDQLKAAGLAVAVALSATGIAGCQSTDDNVLTIGATAAPPTLDLVSNAAAAIPQVLLYN
CCCCHHHCCEEEEEEEHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VYETLVRVNDSGKLVGLLAKSWKLSDDGLRLTFRLDPNARFASGARVTSAAVKASLERMR
HHHHHEEECCCCCEEEEEECCCEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHC
GASASPVNQANLAAIKAIHTPDDSTVILDLSNRDNFLLFNLASTSGVVIDPATKDLATRP
CCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCEEECCCCHHHHCCC
QGSGPYVVTEFTPGHSVTLSPSPRPWHEGNRPTVRFAYYSDPTSQTAALLSGDLDVVSDM
CCCCCEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCHHHHHCC
TTPQALSRFTGNPNYRVLRGTTNGEVVLGMNNTSKALSDRRVRQAILMAIDRKGLLDVVW
CCHHHHHHHCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
NGQGTLIGSMVPPTDPWYTDLSHTWPHDPVRARKLLADAGYGNGLTLRLRTAALPYATAA
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHH
SRVVASELAQVGINVVTDELEFPARWLDVVYTHADYDLTIVAHVEARDIVNWANPDYYWR
HHHHHHHHHHCCCEEEECCCCCCHHHEEEEEEECCCEEEEEEEECHHHHHCCCCCCEEEE
YHNKEFNRLIESARTGPADRTNETMMQASRILATDAAGGFLFLMPKITISKSGITGLQRN
ECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCCCCEEEEEEEEEEECCCCCCCCCC
STSMSFDLTKVRRSK
CCCEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA