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The map label for this gene is prfA [H]

Identifier: 50842731

GI number: 50842731

Start: 1355958

End: 1357043

Strand: Reverse

Name: prfA [H]

Synonym: PPA1252

Alternate gene names: 50842731

Gene position: 1357043-1355958 (Counterclockwise)

Preceding gene: 50842732

Following gene: 50842730

Centisome position: 53.0

GC content: 58.38

Gene sequence:

>1086_bases
ATGAGCGAATCTGCGCAACAGGCGATTGATGAAGTTCGTCGTCGTCTGCGGGAGATCGAGACCCAGTTGGCCGATTCTGC
GGTGCTATCAGACCCCTCTGCTATGGGAAGGCTCGGTAAGCGCCATGCACGCCTTCGCCATGTGGTGTCAGTCGCCGACC
GCGTCGACCAGCTAAGGGCCGACGTTGAGGCAGCCGAGGATCTCACTGAGGAGGATCCCGCTTTTGGGCTGGAAGCTGAG
AGGCTGCGTGGCCAATTGGACCTCGCCAGTACAGAGCTCACCGAGTTACTTGCCCCTTCTGACCCCCTGGATCAGGAAGA
TGCTTTAGTCGAAATCCACTCCGGCGAGGGTGGAGAAGAATCTGCCCTCTTTGCTGCTGATCTCTTACGGATGTATACCC
GGTATGCCGAACGGGTGGGGTGGAGCGTTCGCGAGCTCACCAGCGAACCGACAGATCTGGGAGGATATCGCACGGTCGTG
ATTGCCGTTGCGGGGGTTCCATCGCGCCCAGCCTATGGCTACCTCAAGCATGAAGGTGGCGTCCACCGCGTGCAGCGGGT
GCCAGTGACGGAGTCGTCGGGGCGTATTCATACCTCAGCAGTCGGAGTCCTTGTCATGCCGGATGTCGACGAGACTGAGG
TCGATATTGACCCAGCTGAGGTTCGCGTTGACGTGTACCGGTCTTCGGGGCCAGGCGGCCAAGGAGTCAATACCACCGAT
TCGGCTGTTCGTCTTACCCACTTGCCTACCGGCATCGTCGCCAGCTGCCAAAATGAGCGCAGCCAGTTGCAGAACAAAGC
GGAAGCGATGCGGATGTTGCGGGCAAAAGTCGCGGCCTTAGCGGCGCAACAGGCTGCTGATGAAAATGATCGAATGCGTC
GAGACCAGGTGCGTACTGTCGATAGATCGGCTCGAGTGCGTACCTATAACTTTCCCGAGAATCGCCTGACAGATCATCGT
ATTGGGTACAAGTCCAGGAAACTTGACCAGATTCTGGCTGGCGACCTAGGTGATGTTCTAGACGCGCTCCATGCCGATGA
GGTGAGGCGTCGGATGGCACAACGGCCCGAAGGTCAAGAATCCTAA

Upstream 100 bases:

>100_bases
TCGTCTATACCGATGACCATGACGCCGGGCTGCGGCTCGGCGTCATGGCTCGTTTCGGGGGTACAACACCCGACCCAGGC
AACGCTGGTCAGGAGGGGGC

Downstream 100 bases:

>100_bases
TGGCAGATCCCAGCCCATCTCTCCTCCTGGCACGAGCCACTCGACAACTGACCACCGCGGGGATTGAGACCCCGGCAGCA
GATGCACGCATGTTGCTATG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSESAQQAIDEVRRRLREIETQLADSAVLSDPSAMGRLGKRHARLRHVVSVADRVDQLRADVEAAEDLTEEDPAFGLEAE
RLRGQLDLASTELTELLAPSDPLDQEDALVEIHSGEGGEESALFAADLLRMYTRYAERVGWSVRELTSEPTDLGGYRTVV
IAVAGVPSRPAYGYLKHEGGVHRVQRVPVTESSGRIHTSAVGVLVMPDVDETEVDIDPAEVRVDVYRSSGPGGQGVNTTD
SAVRLTHLPTGIVASCQNERSQLQNKAEAMRMLRAKVAALAAQQAADENDRMRRDQVRTVDRSARVRTYNFPENRLTDHR
IGYKSRKLDQILAGDLGDVLDALHADEVRRRMAQRPEGQES

Sequences:

>Translated_361_residues
MSESAQQAIDEVRRRLREIETQLADSAVLSDPSAMGRLGKRHARLRHVVSVADRVDQLRADVEAAEDLTEEDPAFGLEAE
RLRGQLDLASTELTELLAPSDPLDQEDALVEIHSGEGGEESALFAADLLRMYTRYAERVGWSVRELTSEPTDLGGYRTVV
IAVAGVPSRPAYGYLKHEGGVHRVQRVPVTESSGRIHTSAVGVLVMPDVDETEVDIDPAEVRVDVYRSSGPGGQGVNTTD
SAVRLTHLPTGIVASCQNERSQLQNKAEAMRMLRAKVAALAAQQAADENDRMRRDQVRTVDRSARVRTYNFPENRLTDHR
IGYKSRKLDQILAGDLGDVLDALHADEVRRRMAQRPEGQES
>Mature_360_residues
SESAQQAIDEVRRRLREIETQLADSAVLSDPSAMGRLGKRHARLRHVVSVADRVDQLRADVEAAEDLTEEDPAFGLEAER
LRGQLDLASTELTELLAPSDPLDQEDALVEIHSGEGGEESALFAADLLRMYTRYAERVGWSVRELTSEPTDLGGYRTVVI
AVAGVPSRPAYGYLKHEGGVHRVQRVPVTESSGRIHTSAVGVLVMPDVDETEVDIDPAEVRVDVYRSSGPGGQGVNTTDS
AVRLTHLPTGIVASCQNERSQLQNKAEAMRMLRAKVAALAAQQAADENDRMRRDQVRTVDRSARVRTYNFPENRLTDHRI
GYKSRKLDQILAGDLGDVLDALHADEVRRRMAQRPEGQES

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=267, Percent_Identity=43.0711610486891, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=35.9375, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI166795305, Length=173, Percent_Identity=43.3526011560694, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1787462, Length=349, Percent_Identity=40.6876790830946, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=35.207100591716, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=239, Percent_Identity=39.3305439330544, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=38.4858044164038, Blast_Score=219, Evalue=8e-58,
Organism=Drosophila melanogaster, GI19921226, Length=312, Percent_Identity=38.1410256410256, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39833; Mature: 39702

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESAQQAIDEVRRRLREIETQLADSAVLSDPSAMGRLGKRHARLRHVVSVADRVDQLRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVEAAEDLTEEDPAFGLEAERLRGQLDLASTELTELLAPSDPLDQEDALVEIHSGEGGEE
HHHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCH
SALFAADLLRMYTRYAERVGWSVRELTSEPTDLGGYRTVVIAVAGVPSRPAYGYLKHEGG
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCC
VHRVQRVPVTESSGRIHTSAVGVLVMPDVDETEVDIDPAEVRVDVYRSSGPGGQGVNTTD
HHHHEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCC
SAVRLTHLPTGIVASCQNERSQLQNKAEAMRMLRAKVAALAAQQAADENDRMRRDQVRTV
HHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DRSARVRTYNFPENRLTDHRIGYKSRKLDQILAGDLGDVLDALHADEVRRRMAQRPEGQE
HHCCCEEEECCCHHHCCHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
S
C
>Mature Secondary Structure 
SESAQQAIDEVRRRLREIETQLADSAVLSDPSAMGRLGKRHARLRHVVSVADRVDQLRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVEAAEDLTEEDPAFGLEAERLRGQLDLASTELTELLAPSDPLDQEDALVEIHSGEGGEE
HHHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCH
SALFAADLLRMYTRYAERVGWSVRELTSEPTDLGGYRTVVIAVAGVPSRPAYGYLKHEGG
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCC
VHRVQRVPVTESSGRIHTSAVGVLVMPDVDETEVDIDPAEVRVDVYRSSGPGGQGVNTTD
HHHHEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCC
SAVRLTHLPTGIVASCQNERSQLQNKAEAMRMLRAKVAALAAQQAADENDRMRRDQVRTV
HHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DRSARVRTYNFPENRLTDHRIGYKSRKLDQILAGDLGDVLDALHADEVRRRMAQRPEGQE
HHCCCEEEECCCHHHCCHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA