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The map label for this gene is hemK [H]

Identifier: 50842730

GI number: 50842730

Start: 1355083

End: 1355958

Strand: Reverse

Name: hemK [H]

Synonym: PPA1250

Alternate gene names: 50842730

Gene position: 1355958-1355083 (Counterclockwise)

Preceding gene: 50842731

Following gene: 50842729

Centisome position: 52.96

GC content: 59.93

Gene sequence:

>876_bases
ATGGCAGATCCCAGCCCATCTCTCCTCCTGGCACGAGCCACTCGACAACTGACCACCGCGGGGATTGAGACCCCGGCAGC
AGATGCACGCATGTTGCTATGTGAAGCACTGAGAATCCAACCGTCACAACTCATTCGTGTGGCGAGCGTTAATGCTGATG
ATGAGGATCGTTTCAATCAGATGGTGGATCGGCGGCGAAGCGGGGAACCAGCGCAGTACATCATCGGTCATGCCTGGTTC
CGTGGCCTTCGGCTGGAGGTCGGGCCAGGAGTGTTTATACCCCGCTTGGAGACCGAGTTGGTGGCCGAACAGTCCATCCA
GGAGGCTCGACGATTGGTGATGTCTGGCGCTTGCCCTAGTGTGATCGATTTGTGCACCGGTACGGGAGCTATTGCTCTTG
CGGTGGCTAGTGAAGTCCCGGGTTCCCGGGTCAGCGCCGTCGAAGTGGATGACGCTGCTCTGACGTGGACCCGACGCAAC
CTGTGCGACTCCGGAGTTGAGGTTCTCGCGGGCGACGCTTTGCGGGTACCTGACGATGGCCGACGGTTTGACGTCGTCGT
CACAAATCCGCCGTACTTGCGTCGTAGTGATGTTTCGTCTATCCCTGGGGAAGTCACCGAACACGAGCCCGACCTTGCCT
TATTCTCTGGTGACGACGGGCTCGACCTACCGCTGCTCCTCATCGGGCGAGCTGCCGAACTGCTTACGCCGGGCGGCCTG
TTCGTCATGGAACACGACGAGACCCAACGTGAGGAGCTCGTCGCTGCGATGGCGACTTCCGATATGTGGGAGCAGATCGA
GGACCATGATGATCTGGCAGGTAGGCCGAGGTTCGTCACGGCTCGCCGACGGTGCAAGGATGGATGCCATGGATGA

Upstream 100 bases:

>100_bases
ACCAGATTCTGGCTGGCGACCTAGGTGATGTTCTAGACGCGCTCCATGCCGATGAGGTGAGGCGTCGGATGGCACAACGG
CCCGAAGGTCAAGAATCCTA

Downstream 100 bases:

>100_bases
GACTGACCAGACGATGGTCAATACCGACGAGCTGGCCGGGCCGGTCGCAGAAGAGAAGTCGACAGTCAGCGGTATCGAGC
CAAGAGGAGACGCAGCGGCT

Product: putative methylase

Products: NA

Alternate protein names: M.MleHemKP [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MADPSPSLLLARATRQLTTAGIETPAADARMLLCEALRIQPSQLIRVASVNADDEDRFNQMVDRRRSGEPAQYIIGHAWF
RGLRLEVGPGVFIPRLETELVAEQSIQEARRLVMSGACPSVIDLCTGTGAIALAVASEVPGSRVSAVEVDDAALTWTRRN
LCDSGVEVLAGDALRVPDDGRRFDVVVTNPPYLRRSDVSSIPGEVTEHEPDLALFSGDDGLDLPLLLIGRAAELLTPGGL
FVMEHDETQREELVAAMATSDMWEQIEDHDDLAGRPRFVTARRRCKDGCHG

Sequences:

>Translated_291_residues
MADPSPSLLLARATRQLTTAGIETPAADARMLLCEALRIQPSQLIRVASVNADDEDRFNQMVDRRRSGEPAQYIIGHAWF
RGLRLEVGPGVFIPRLETELVAEQSIQEARRLVMSGACPSVIDLCTGTGAIALAVASEVPGSRVSAVEVDDAALTWTRRN
LCDSGVEVLAGDALRVPDDGRRFDVVVTNPPYLRRSDVSSIPGEVTEHEPDLALFSGDDGLDLPLLLIGRAAELLTPGGL
FVMEHDETQREELVAAMATSDMWEQIEDHDDLAGRPRFVTARRRCKDGCHG
>Mature_290_residues
ADPSPSLLLARATRQLTTAGIETPAADARMLLCEALRIQPSQLIRVASVNADDEDRFNQMVDRRRSGEPAQYIIGHAWFR
GLRLEVGPGVFIPRLETELVAEQSIQEARRLVMSGACPSVIDLCTGTGAIALAVASEVPGSRVSAVEVDDAALTWTRRNL
CDSGVEVLAGDALRVPDDGRRFDVVVTNPPYLRRSDVSSIPGEVTEHEPDLALFSGDDGLDLPLLLIGRAAELLTPGGLF
VMEHDETQREELVAAMATSDMWEQIEDHDDLAGRPRFVTARRRCKDGCHG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=242, Percent_Identity=30.9917355371901, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI87082085, Length=234, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787463, Length=253, Percent_Identity=32.4110671936759, Blast_Score=97, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24582226, Length=298, Percent_Identity=23.8255033557047, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31677; Mature: 31546

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPSPSLLLARATRQLTTAGIETPAADARMLLCEALRIQPSQLIRVASVNADDEDRFNQ
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHEEEEEECCCCCHHHHHH
MVDRRRSGEPAQYIIGHAWFRGLRLEVGPGVFIPRLETELVAEQSIQEARRLVMSGACPS
HHHHHCCCCCHHHHHHHHHHHCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHCCCCHH
VIDLCTGTGAIALAVASEVPGSRVSAVEVDDAALTWTRRNLCDSGVEVLAGDALRVPDDG
HHHHHCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCHHHHCCCEEECCCCC
RRFDVVVTNPPYLRRSDVSSIPGEVTEHEPDLALFSGDDGLDLPLLLIGRAAELLTPGGL
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCE
FVMEHDETQREELVAAMATSDMWEQIEDHDDLAGRPRFVTARRRCKDGCHG
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADPSPSLLLARATRQLTTAGIETPAADARMLLCEALRIQPSQLIRVASVNADDEDRFNQ
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHEEEEEECCCCCHHHHHH
MVDRRRSGEPAQYIIGHAWFRGLRLEVGPGVFIPRLETELVAEQSIQEARRLVMSGACPS
HHHHHCCCCCHHHHHHHHHHHCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHCCCCHH
VIDLCTGTGAIALAVASEVPGSRVSAVEVDDAALTWTRRNLCDSGVEVLAGDALRVPDDG
HHHHHCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCHHHHCCCEEECCCCC
RRFDVVVTNPPYLRRSDVSSIPGEVTEHEPDLALFSGDDGLDLPLLLIGRAAELLTPGGL
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCE
FVMEHDETQREELVAAMATSDMWEQIEDHDDLAGRPRFVTARRRCKDGCHG
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]