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The map label for this gene is aspS

Identifier: 50842655

GI number: 50842655

Start: 1280167

End: 1281987

Strand: Direct

Name: aspS

Synonym: PPA1175

Alternate gene names: 50842655

Gene position: 1280167-1281987 (Clockwise)

Preceding gene: 50842654

Following gene: 50842656

Centisome position: 50.0

GC content: 61.12

Gene sequence:

>1821_bases
GTGCTGCGAACCCATGACGCAGGAACCCTACGAGCCAGCGACATCGGCAAGGGAGTGACTCTCGCCGGATGGGTTGGGCA
TCGTCGCGACCACGGTGGCGTCGCCTTCATCGATCTTCGCGACGCCTCAGGCCTGGCCCAGGTCGTCATCCGCGATGAGA
TTTTGGAGTCCTCAGGCGCTCACGACCTGCGCAACGAGTACTGCATCAGGGTGACCGGAGTCGTTGAGGCACGTCCAGAG
GGTAATGCCAACCCGAACCTACCCACCGGCGAGATTGAGGTTGCCATCACCGAGCTCGAGGTGCTCAATCCTTCAGCGCC
GCTACCCTTCCAGATCGACGAGCACGTCAGTGTCGGCGAGGACGCCCGTCTGAAGTACCGCTACCTCGACCTGCGCCGCC
CGCATCAGCATGAAGCCCTGGTGCTGCGTTCTCACGTGACCCACGCCATTCGTGAGGTGCTGGATCGTCACAATTTTTAT
GACATCGAGACTCCGACTCTGACCCGGTCTACCCCTGAGGGCGCTCGCGACTTTCTGGTTCCGGCTCGTCTGGCCCCGGG
CTGTTGGTACGCCCTGCCGCAGAGCCCTCAGTTGTTCAAACAGCTGCTCATGGTCTCGGGTATGGAACGTTATTACCAGA
TCGCTCGTTGCTACCGTGACGAGGACTTCCGTGCCGATCGTCAGCCTGAGTTCACCCAGCTTGATGTCGAGATGAGCTTC
GTTGACCAGGACGATGTCATCGCGCTGGCTGAAGAGACCATGGCGGCTTGTTGGAAGATCATTGGCGTCGACATGCCGAC
GCCGTTACCTCGCATCACCTGGCACGAGGCGATGGATCGCTACGGCTCTGACAAGCCGGATCTGCGCTTTGGCAACGAGA
TCACCGAGGTTACCGATTTCTTTGCCAACACTGCGTTCCGGGTCTTTCAGGCTCCCTACGTCGGTGCCGTGGTGATGCCC
GGCGGAGCCTCCCTGCCACGCCGTCAGTTCGACGCCTGGCAGGAGTGGGCGCGCACCCGCGGAGCCAAGGGTCTGGCCTA
CATCACGATCTCTGACGAGGGCGTGCCAGAAGGTCCAGTTGCCAAAAACATTTCCGAGGAGGAGAAGGCTGGCATCGCCG
AGAAGGTCGGTGCCAAGCCTGGTGATGCCATCTTCTTTGCTGCTGGCTCGAAGACCTCCTCCCAAGAGCTACTCGGCGCC
GCCCGTCTGGAAATCGGCCGCCGTTGCGAACTCTTTGATCCATCTGACTGGGCTTTCTGCTGGGTGGTTGACGCCCCGTT
GTTCAAGCCCACCAAGGAAGCAGAGGCTTCCGGCGACGTCGCCGTTGGCACGGGCGCCTGGACCGCCGTCCACCACGCCT
TCACCAGCCCGACCCCCGACTGCCTGGATACCTTCGACACCGATCCGGGTTCAGCTTTGGCTTACGCTTACGACTTCGTC
GTCAACGGCAACGAGGTAGGCGGCGGGTCGATCCGTATCCATCGCCGTGACGTTCAGGAGCGGGTCTTCAACGTCATGGG
TATTGGCCCCGAAGAGGCCCAAGAGAAATTCGGTTTCCTACTGGATGCCTTCAAATTTGGCGCGCCGCCGCACGGTGGCA
TCGCCTTCGGTTTGGATCGTCTAGTTATGCTGCTTGGCGGCTTCGAGACTATCCGTGACGTTATTGCCTTCCCGAAGACT
GGTAACGGCTATGACCCGCTGACGGCTGCTCCTGCACCAATTACCCCTGCCCAGCGGCGCGAAGCCGGCGTCGATGCCAA
GCCGAAGGAGCCTTCCGGCGGTCAAACCTCGAATGCGGAGGCATATAAGTCCACGACGTGA

Upstream 100 bases:

>100_bases
TGGGCATCTGGGTGCGGATGGCGACAGTCATGGCCCGTCGGTATGCTGTTTTACGGCCCGTACCGTCTATGCGTGGCCTG
CGACAACGGAAGGATGAAAC

Downstream 100 bases:

>100_bases
CGACCGGTGACGACGGGCGGCGGCGATATCAGTCGCTGCCCGAGCGTTACTGCGCGAACATACGTGAGCGGCTCGCTGTC
GCAACGCGGCGATATGGTGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MLRTHDAGTLRASDIGKGVTLAGWVGHRRDHGGVAFIDLRDASGLAQVVIRDEILESSGAHDLRNEYCIRVTGVVEARPE
GNANPNLPTGEIEVAITELEVLNPSAPLPFQIDEHVSVGEDARLKYRYLDLRRPHQHEALVLRSHVTHAIREVLDRHNFY
DIETPTLTRSTPEGARDFLVPARLAPGCWYALPQSPQLFKQLLMVSGMERYYQIARCYRDEDFRADRQPEFTQLDVEMSF
VDQDDVIALAEETMAACWKIIGVDMPTPLPRITWHEAMDRYGSDKPDLRFGNEITEVTDFFANTAFRVFQAPYVGAVVMP
GGASLPRRQFDAWQEWARTRGAKGLAYITISDEGVPEGPVAKNISEEEKAGIAEKVGAKPGDAIFFAAGSKTSSQELLGA
ARLEIGRRCELFDPSDWAFCWVVDAPLFKPTKEAEASGDVAVGTGAWTAVHHAFTSPTPDCLDTFDTDPGSALAYAYDFV
VNGNEVGGGSIRIHRRDVQERVFNVMGIGPEEAQEKFGFLLDAFKFGAPPHGGIAFGLDRLVMLLGGFETIRDVIAFPKT
GNGYDPLTAAPAPITPAQRREAGVDAKPKEPSGGQTSNAEAYKSTT

Sequences:

>Translated_606_residues
MLRTHDAGTLRASDIGKGVTLAGWVGHRRDHGGVAFIDLRDASGLAQVVIRDEILESSGAHDLRNEYCIRVTGVVEARPE
GNANPNLPTGEIEVAITELEVLNPSAPLPFQIDEHVSVGEDARLKYRYLDLRRPHQHEALVLRSHVTHAIREVLDRHNFY
DIETPTLTRSTPEGARDFLVPARLAPGCWYALPQSPQLFKQLLMVSGMERYYQIARCYRDEDFRADRQPEFTQLDVEMSF
VDQDDVIALAEETMAACWKIIGVDMPTPLPRITWHEAMDRYGSDKPDLRFGNEITEVTDFFANTAFRVFQAPYVGAVVMP
GGASLPRRQFDAWQEWARTRGAKGLAYITISDEGVPEGPVAKNISEEEKAGIAEKVGAKPGDAIFFAAGSKTSSQELLGA
ARLEIGRRCELFDPSDWAFCWVVDAPLFKPTKEAEASGDVAVGTGAWTAVHHAFTSPTPDCLDTFDTDPGSALAYAYDFV
VNGNEVGGGSIRIHRRDVQERVFNVMGIGPEEAQEKFGFLLDAFKFGAPPHGGIAFGLDRLVMLLGGFETIRDVIAFPKT
GNGYDPLTAAPAPITPAQRREAGVDAKPKEPSGGQTSNAEAYKSTT
>Mature_606_residues
MLRTHDAGTLRASDIGKGVTLAGWVGHRRDHGGVAFIDLRDASGLAQVVIRDEILESSGAHDLRNEYCIRVTGVVEARPE
GNANPNLPTGEIEVAITELEVLNPSAPLPFQIDEHVSVGEDARLKYRYLDLRRPHQHEALVLRSHVTHAIREVLDRHNFY
DIETPTLTRSTPEGARDFLVPARLAPGCWYALPQSPQLFKQLLMVSGMERYYQIARCYRDEDFRADRQPEFTQLDVEMSF
VDQDDVIALAEETMAACWKIIGVDMPTPLPRITWHEAMDRYGSDKPDLRFGNEITEVTDFFANTAFRVFQAPYVGAVVMP
GGASLPRRQFDAWQEWARTRGAKGLAYITISDEGVPEGPVAKNISEEEKAGIAEKVGAKPGDAIFFAAGSKTSSQELLGA
ARLEIGRRCELFDPSDWAFCWVVDAPLFKPTKEAEASGDVAVGTGAWTAVHHAFTSPTPDCLDTFDTDPGSALAYAYDFV
VNGNEVGGGSIRIHRRDVQERVFNVMGIGPEEAQEKFGFLLDAFKFGAPPHGGIAFGLDRLVMLLGGFETIRDVIAFPKT
GNGYDPLTAAPAPITPAQRREAGVDAKPKEPSGGQTSNAEAYKSTT

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=596, Percent_Identity=41.1073825503356, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI45439306, Length=309, Percent_Identity=24.9190938511327, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI4758762, Length=242, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI5031815, Length=266, Percent_Identity=26.6917293233083, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI194272210, Length=266, Percent_Identity=26.6917293233083, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1788173, Length=601, Percent_Identity=48.585690515807, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1790571, Length=287, Percent_Identity=27.8745644599303, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789256, Length=288, Percent_Identity=28.4722222222222, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=35.4785478547855, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17551876, Length=255, Percent_Identity=26.2745098039216, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=231, Percent_Identity=26.4069264069264, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=217, Percent_Identity=24.4239631336406, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=217, Percent_Identity=24.4239631336406, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=217, Percent_Identity=24.4239631336406, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=644, Percent_Identity=31.2111801242236, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6321807, Length=312, Percent_Identity=22.7564102564103, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=278, Percent_Identity=25.5395683453237, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=174, Percent_Identity=26.4367816091954, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=619, Percent_Identity=39.7415185783522, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17136276, Length=253, Percent_Identity=26.4822134387352, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=25.4980079681275, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24640849, Length=251, Percent_Identity=25.4980079681275, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19921528, Length=225, Percent_Identity=27.1111111111111, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_PROAC (Q6A8J1)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055882.1
- ProteinModelPortal:   Q6A8J1
- SMR:   Q6A8J1
- GeneID:   2932005
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1175
- NMPDR:   fig|267747.1.peg.1159
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   PACN267747:PPA1175-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66516; Mature: 66516

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTHDAGTLRASDIGKGVTLAGWVGHRRDHGGVAFIDLRDASGLAQVVIRDEILESSGA
CCCCCCCCCEEHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
HDLRNEYCIRVTGVVEARPEGNANPNLPTGEIEVAITELEVLNPSAPLPFQIDEHVSVGE
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEECCCCCCCC
DARLKYRYLDLRRPHQHEALVLRSHVTHAIREVLDRHNFYDIETPTLTRSTPEGARDFLV
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHCCC
PARLAPGCWYALPQSPQLFKQLLMVSGMERYYQIARCYRDEDFRADRQPEFTQLDVEMSF
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEE
VDQDDVIALAEETMAACWKIIGVDMPTPLPRITWHEAMDRYGSDKPDLRFGNEITEVTDF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
FANTAFRVFQAPYVGAVVMPGGASLPRRQFDAWQEWARTRGAKGLAYITISDEGVPEGPV
HHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
AKNISEEEKAGIAEKVGAKPGDAIFFAAGSKTSSQELLGAARLEIGRRCELFDPSDWAFC
CCCCCHHHHCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEE
WVVDAPLFKPTKEAEASGDVAVGTGAWTAVHHAFTSPTPDCLDTFDTDPGSALAYAYDFV
EEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEEE
VNGNEVGGGSIRIHRRDVQERVFNVMGIGPEEAQEKFGFLLDAFKFGAPPHGGIAFGLDR
EECCEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
LVMLLGGFETIRDVIAFPKTGNGYDPLTAAPAPITPAQRREAGVDAKPKEPSGGQTSNAE
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
AYKSTT
HHCCCC
>Mature Secondary Structure
MLRTHDAGTLRASDIGKGVTLAGWVGHRRDHGGVAFIDLRDASGLAQVVIRDEILESSGA
CCCCCCCCCEEHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
HDLRNEYCIRVTGVVEARPEGNANPNLPTGEIEVAITELEVLNPSAPLPFQIDEHVSVGE
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEECCCCCCCC
DARLKYRYLDLRRPHQHEALVLRSHVTHAIREVLDRHNFYDIETPTLTRSTPEGARDFLV
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHCCC
PARLAPGCWYALPQSPQLFKQLLMVSGMERYYQIARCYRDEDFRADRQPEFTQLDVEMSF
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEE
VDQDDVIALAEETMAACWKIIGVDMPTPLPRITWHEAMDRYGSDKPDLRFGNEITEVTDF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
FANTAFRVFQAPYVGAVVMPGGASLPRRQFDAWQEWARTRGAKGLAYITISDEGVPEGPV
HHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
AKNISEEEKAGIAEKVGAKPGDAIFFAAGSKTSSQELLGAARLEIGRRCELFDPSDWAFC
CCCCCHHHHCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEE
WVVDAPLFKPTKEAEASGDVAVGTGAWTAVHHAFTSPTPDCLDTFDTDPGSALAYAYDFV
EEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEEE
VNGNEVGGGSIRIHRRDVQERVFNVMGIGPEEAQEKFGFLLDAFKFGAPPHGGIAFGLDR
EECCEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
LVMLLGGFETIRDVIAFPKTGNGYDPLTAAPAPITPAQRREAGVDAKPKEPSGGQTSNAE
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
AYKSTT
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA