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The map label for this gene is ywbA [H]

Identifier: 50842627

GI number: 50842627

Start: 1246988

End: 1248256

Strand: Direct

Name: ywbA [H]

Synonym: PPA1148

Alternate gene names: 50842627

Gene position: 1246988-1248256 (Clockwise)

Preceding gene: 50842626

Following gene: 50842628

Centisome position: 48.71

GC content: 49.8

Gene sequence:

>1269_bases
ATGGAGAAGTTCTTTAACTTCTTGGATAGGAGAGTGGCTGCTCCGATGTCGATGATATCGGAGCAGCGACATATCCGGGC
TATTCGCGACGGTGTAATTTCCGCTATCCCCTTCATCATCGCCGGCAGCTTAATTCTTATTATAGCAGCTCCGCCGGTCC
CTGAAACGTCAGGATTTGCTATGTGGGTGAAGGACCACGCGGACCAGATTCTCATTCCATACCGTATGACGTTCGGGATC
ATGTCCCTATATGTATGCTTCGGAGTCGGTTCAAGTCTTGCTCGTAGTTATGATCTCGATGGCCTTGCTGGTGGCCAACT
TGGTGTCGCTGCATTTCTACTTAGCCTGACCCCAAAGACGTTTGGAGATGGAATCTACGTCGCACTTGAGAGTCTAGGCT
CAAGAGGGCTATTCCCTGCGATGATCTTAGCACTGCTGGCCGTTGAAGTGATGCGCATCTGTTATAAGCATAACTTGACA
TTCAGGATGCCAGAACAGGTTCCTGAGTCCGTCAGCCGATCTTTCGGTGCCGTCGTTCCGGCCTTCATTATCATGGGTAT
AATGACCCTCATATCTGTGGTTTTTAAGATCGACATCGTCGAGGTTGTGCAGCAGGGTCTTTCACCTCTAGTTAAAGCTG
GCGATTCGTTACCAGGAGTATTAGTACCCGCTTTCTTAGTCGTGTTTCTATGGTTCTTCGGGATCAGCGGAGATTCGGTA
GTTAGATCGGTTGCCCGGCCGGTGTGGTTGCAGTATCTGAGCGACAACGCTGACGCCGTGGCTTCTGGTGTCCCGGCCCC
TCACATTGCACCGGAGACGTTCTTCCAGTGGTTCGTCTCCATTGGTGGCTCTGGGGCGACTATGGGTCTAGTTATTGCCG
GTTTTTTAGTTGGGAGAGCAAGGTATACCAAGTCAATCATGCGAGCGGTCGCGGTTCCTGCGTTGTTCAATATTAGTGAG
CCGATCATGTTCGGTCTCCCAGTCATGATGAATCCGCTTTTTATTATACCTTTCCTCCTCGCGCCGTTGGTGCTATGCGT
GGTAACTTGGTTTGCTTTTATTTGGGGTTTTGTCACGTATATGGCTGTTCAGCCGCCGTGGACGTTACCCGCTCCAATAG
GCGCGTTTTTGACGACGGGCGGCGACTGGCGTGCCATCGTTTTATGCCTTGTAAATATATTAATTTCTACTGCTATTTAT
TATCCATTTATGAGGATGTATGACAGGGATCAACTCAGAAAAGAGCAATGCGAAGAAGGTGGAGCATGA

Upstream 100 bases:

>100_bases
GACGTGGGATCCCGATTTGCGCGATTCCTCCGGTTGCCTACGGTCCGCTTGGCGGTTCAGAACTGGTAAAGACTGTCAAA
GAACATCTCGGGTGATCGCC

Downstream 100 bases:

>100_bases
CTTCATATTGTGGGAGTGACAGCCCGGATGGTGGGGCGGTGATGCCAGAGATCGAAACCGCCATTTTTACCATCATCACG
AGCGCTGGTGAGGCGCGTGC

Product: PTS system, cellobiose-specific IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Putative PTS system EIIC component [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MEKFFNFLDRRVAAPMSMISEQRHIRAIRDGVISAIPFIIAGSLILIIAAPPVPETSGFAMWVKDHADQILIPYRMTFGI
MSLYVCFGVGSSLARSYDLDGLAGGQLGVAAFLLSLTPKTFGDGIYVALESLGSRGLFPAMILALLAVEVMRICYKHNLT
FRMPEQVPESVSRSFGAVVPAFIIMGIMTLISVVFKIDIVEVVQQGLSPLVKAGDSLPGVLVPAFLVVFLWFFGISGDSV
VRSVARPVWLQYLSDNADAVASGVPAPHIAPETFFQWFVSIGGSGATMGLVIAGFLVGRARYTKSIMRAVAVPALFNISE
PIMFGLPVMMNPLFIIPFLLAPLVLCVVTWFAFIWGFVTYMAVQPPWTLPAPIGAFLTTGGDWRAIVLCLVNILISTAIY
YPFMRMYDRDQLRKEQCEEGGA

Sequences:

>Translated_422_residues
MEKFFNFLDRRVAAPMSMISEQRHIRAIRDGVISAIPFIIAGSLILIIAAPPVPETSGFAMWVKDHADQILIPYRMTFGI
MSLYVCFGVGSSLARSYDLDGLAGGQLGVAAFLLSLTPKTFGDGIYVALESLGSRGLFPAMILALLAVEVMRICYKHNLT
FRMPEQVPESVSRSFGAVVPAFIIMGIMTLISVVFKIDIVEVVQQGLSPLVKAGDSLPGVLVPAFLVVFLWFFGISGDSV
VRSVARPVWLQYLSDNADAVASGVPAPHIAPETFFQWFVSIGGSGATMGLVIAGFLVGRARYTKSIMRAVAVPALFNISE
PIMFGLPVMMNPLFIIPFLLAPLVLCVVTWFAFIWGFVTYMAVQPPWTLPAPIGAFLTTGGDWRAIVLCLVNILISTAIY
YPFMRMYDRDQLRKEQCEEGGA
>Mature_422_residues
MEKFFNFLDRRVAAPMSMISEQRHIRAIRDGVISAIPFIIAGSLILIIAAPPVPETSGFAMWVKDHADQILIPYRMTFGI
MSLYVCFGVGSSLARSYDLDGLAGGQLGVAAFLLSLTPKTFGDGIYVALESLGSRGLFPAMILALLAVEVMRICYKHNLT
FRMPEQVPESVSRSFGAVVPAFIIMGIMTLISVVFKIDIVEVVQQGLSPLVKAGDSLPGVLVPAFLVVFLWFFGISGDSV
VRSVARPVWLQYLSDNADAVASGVPAPHIAPETFFQWFVSIGGSGATMGLVIAGFLVGRARYTKSIMRAVAVPALFNISE
PIMFGLPVMMNPLFIIPFLLAPLVLCVVTWFAFIWGFVTYMAVQPPWTLPAPIGAFLTTGGDWRAIVLCLVNILISTAIY
YPFMRMYDRDQLRKEQCEEGGA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=429, Percent_Identity=31.4685314685315, Blast_Score=201, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 46068; Mature: 46068

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFFNFLDRRVAAPMSMISEQRHIRAIRDGVISAIPFIIAGSLILIIAAPPVPETSGFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCE
MWVKDHADQILIPYRMTFGIMSLYVCFGVGSSLARSYDLDGLAGGQLGVAAFLLSLTPKT
EEEECCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHH
FGDGIYVALESLGSRGLFPAMILALLAVEVMRICYKHNLTFRMPEQVPESVSRSFGAVVP
HCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHH
AFIIMGIMTLISVVFKIDIVEVVQQGLSPLVKAGDSLPGVLVPAFLVVFLWFFGISGDSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
VRSVARPVWLQYLSDNADAVASGVPAPHIAPETFFQWFVSIGGSGATMGLVIAGFLVGRA
HHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RYTKSIMRAVAVPALFNISEPIMFGLPVMMNPLFIIPFLLAPLVLCVVTWFAFIWGFVTY
HHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAVQPPWTLPAPIGAFLTTGGDWRAIVLCLVNILISTAIYYPFMRMYDRDQLRKEQCEEG
HHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
GA
CC
>Mature Secondary Structure
MEKFFNFLDRRVAAPMSMISEQRHIRAIRDGVISAIPFIIAGSLILIIAAPPVPETSGFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCE
MWVKDHADQILIPYRMTFGIMSLYVCFGVGSSLARSYDLDGLAGGQLGVAAFLLSLTPKT
EEEECCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHH
FGDGIYVALESLGSRGLFPAMILALLAVEVMRICYKHNLTFRMPEQVPESVSRSFGAVVP
HCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHH
AFIIMGIMTLISVVFKIDIVEVVQQGLSPLVKAGDSLPGVLVPAFLVVFLWFFGISGDSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
VRSVARPVWLQYLSDNADAVASGVPAPHIAPETFFQWFVSIGGSGATMGLVIAGFLVGRA
HHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RYTKSIMRAVAVPALFNISEPIMFGLPVMMNPLFIIPFLLAPLVLCVVTWFAFIWGFVTY
HHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAVQPPWTLPAPIGAFLTTGGDWRAIVLCLVNILISTAIYYPFMRMYDRDQLRKEQCEEG
HHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
GA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]