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The map label for this gene is ycjT [C]

Identifier: 50842587

GI number: 50842587

Start: 1200468

End: 1202975

Strand: Direct

Name: ycjT [C]

Synonym: PPA1108

Alternate gene names: 50842587

Gene position: 1200468-1202975 (Clockwise)

Preceding gene: 50842586

Following gene: 50842595

Centisome position: 46.89

GC content: 56.46

Gene sequence:

>2508_bases
ATGACGCGGACGGGCACTGACCCGATGGATCGATGGCGCTTTCCCGACGATCCGTGGGCGATTGTTGAGACTGCCCCCAG
CACAGATGATCTAGGAACTACTGAGACGCTGTTTTCAATTGGTAATGGCTATCTCGGTATGCGTGGTAATCCGTCGGAAG
GCCGCGACTCTTTCAGTCACGGCACCTACATCAACGGGTTCCATGAAATTTGGGACATCCATCACGCTGAGAATGCCTAC
GGTTTCGCGCGTACCGGTCAGACGATTGTCAACGTTCCGGACGCCAAGCTTATGAAGCTCTACGTGGATGACGAACCGCT
CCTGCTCAGCATCAACGAGATCCAGTCGTATAAACGGTGGATCGACTTCCGCGAGGGTGTCCTGCGTCGGGAGCTGGTAT
GGCGCACTCCGGCGGGCAAGTTGCTGCGGGTGGCAACCTCGCGGATGGTCTCCTTCACTCACCGGCACATGGCTCTAATT
AGCATCGAGGTGACGATGCTCGAGGGCGACGCCCCGATCGTCATTAGCTCGCAAATCCTTAACCGTCAGGACGGCATGGA
CGAGTACCACGCTCGACCTGACGACATCGAGAAGACTGCCGACCCTCGCCAAGCTCGTAAATTTGCCGACAAGGTACTCA
TTCCTGAAAAAGATTGGCACTCTGACAGGCGCATGATTTTGGGATTCCGCACCGCACGTTCGGGGATGACCCTGGCTGTA
GGCGCCGATCACGAGATCATCACGGAAAATGAGTACGCGGCCCTTATCGATACCGAGCCCGGGATGGGAAAACGGGTGTA
TCGTGTTGAGGCCACCCAAGGCCGACCAATTCGCATCGATAAGGCGGTTGCTTATCACACTTCTCGCGGCGTGCCGGTAC
ATGAACTGTTTGACCGAGTGCGCCGCAGCTTAGATCGAGTGCGTGAACAGGGGCACAACGTCTACTACGACGAACAACGT
GCATGGCTTGATGATTACTGGGCAACGGCTGATGTCGAGGTCGAAGGTGCCCCGACCGGTATTCAGCAGGCTGTCAGGTG
GAACCTTTTCCAGATTGCTCAGGCATCAGCCCGTGCAGATCAACTTGGCATTCCGGCAAAGGGTGTAACCGGGTCAGGCT
ATGAAGGCCACTACTTTTGGGACACTGAGGTTTATGTCATCCCGATGCTGACCTACACTCATCCAAGAATCGCTGAGAAT
GCGCTGAGATTTCGGGTGAATACCCTTCCGCAAGCTCGACGTCGGGCTAAGGAGTTGTCTGAACGAGGTGCCCTTTTCCC
GTGGCGAACAATCACCGGTGAGGAGGCGTCAGCTTATTACGCGGCGGGTACTGCTCAATACCACATCAATGCCGATATTG
TCCATGCGATTATGAACCACGCCCGGGCGACCGAAGATAAGACCTTCCTCTTCCGGGACGCGGCACCGGTCCTCGTCGAG
ACCGCACGAATGTGGGCTGATCTTGGGTTCTGGCGCATCAACGGTGGTCGAGAGTTCCACATCCACGGGGTTACCGGGCC
CGACGAGTACACCACTGTCGTTAATAATAACCTCTACACCAACGTTATGGCGAGGGCCAACCTCATCGATGCCGCCGGTG
TTATTAGGCGTATGCGTGACGAGGACCCACTGTGGTACGAGCACCTGTGTTCAGAGTTGGACCTCACCGAAGATGAAGTT
GGTGGCTGGGAGGAGTGTGCGGCGGGGATGGTGATCCCGTTCGACGACACCTTCGGTATCCATCCGCAGGATGACCAATT
CCTTTCACGTGAGCTTTGGGACCTCAAGAACACCCCTGGTAACAAGCGGCCGCTGTTGCTGCATTATCACCCGCTGGTGA
TCTACCGATTCCAGGTACTCAAGCAGGCAGACGTTGTGCTGGCTTTGTTCCTGCAGGGAGATCAATTCACCCAGGCTGTC
AAGCTTGCTGACTTTGAGTATTACGACCCCATCACCACCGGTGATTCGAGTCTGTCGGCGGTAGTCCAGTCGGTGATGGC
GGCAGAAGTTGGCCATCAGGAGATGGCGATGGGCTATTTTCTCGACGCGCTCTTCTGCGACCTGGCTGATACCCATCACA
ACGCTTCGGACGGAGTTCACGTTGCCTCGACCGGTGGAGTGTGGGGATGCCTCGTGCACGGTTTCGTTGGCATGCGCAAT
GACCGGGAGAACTTGCGTTTTGATCCGAGACTTCCAGCCCAATGGACGTCGATCAAACACCACATGCTCATTGGCGACTC
TCACATGCTCGTTTTCCTGGAACGTGACGCCATTACGTTCCAGATTCTGTCGGGCCATGACCGCGACGTGACAGTGCGCG
GTGAGCTCTACCACATTGGGGTTGAGCCGGTGAGGGTGCCGTTGTCCGATCAGGGGCCGTTGCGTCCTAGCCTGCGGGTT
ACCCATCCGATCTCGGGTTTGCGTCGAGCTGACGGTTCTCTTATCACTGCAGAAGTTCCCGGCAGCATTGCTGAGACGAT
TGTTTCTTCTCCGATCTCGACGTCGTGA

Upstream 100 bases:

>100_bases
TGGTGCTTGGCGTCAACCGTGGTGTCGGGGCCGACCGGCTGCGTGAGGCCGGCGCCGACCGGGTGGTGGATGACTTAGAT
GAGATGGTTGAGGAGATGGC

Downstream 100 bases:

>100_bases
GACTGATAACGACGGGAGGAGGCCGGCACCATTCGCGGTGTCGGCCTCCTCCATATCGTGGAGGTACGGTGCGGTCCGGT
TACCCGGTTTGCCTCGAACT

Product: trehalose phosphorylase

Products: D-glucose; beta-D-glucose 1-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 835; Mature: 834

Protein sequence:

>835_residues
MTRTGTDPMDRWRFPDDPWAIVETAPSTDDLGTTETLFSIGNGYLGMRGNPSEGRDSFSHGTYINGFHEIWDIHHAENAY
GFARTGQTIVNVPDAKLMKLYVDDEPLLLSINEIQSYKRWIDFREGVLRRELVWRTPAGKLLRVATSRMVSFTHRHMALI
SIEVTMLEGDAPIVISSQILNRQDGMDEYHARPDDIEKTADPRQARKFADKVLIPEKDWHSDRRMILGFRTARSGMTLAV
GADHEIITENEYAALIDTEPGMGKRVYRVEATQGRPIRIDKAVAYHTSRGVPVHELFDRVRRSLDRVREQGHNVYYDEQR
AWLDDYWATADVEVEGAPTGIQQAVRWNLFQIAQASARADQLGIPAKGVTGSGYEGHYFWDTEVYVIPMLTYTHPRIAEN
ALRFRVNTLPQARRRAKELSERGALFPWRTITGEEASAYYAAGTAQYHINADIVHAIMNHARATEDKTFLFRDAAPVLVE
TARMWADLGFWRINGGREFHIHGVTGPDEYTTVVNNNLYTNVMARANLIDAAGVIRRMRDEDPLWYEHLCSELDLTEDEV
GGWEECAAGMVIPFDDTFGIHPQDDQFLSRELWDLKNTPGNKRPLLLHYHPLVIYRFQVLKQADVVLALFLQGDQFTQAV
KLADFEYYDPITTGDSSLSAVVQSVMAAEVGHQEMAMGYFLDALFCDLADTHHNASDGVHVASTGGVWGCLVHGFVGMRN
DRENLRFDPRLPAQWTSIKHHMLIGDSHMLVFLERDAITFQILSGHDRDVTVRGELYHIGVEPVRVPLSDQGPLRPSLRV
THPISGLRRADGSLITAEVPGSIAETIVSSPISTS

Sequences:

>Translated_835_residues
MTRTGTDPMDRWRFPDDPWAIVETAPSTDDLGTTETLFSIGNGYLGMRGNPSEGRDSFSHGTYINGFHEIWDIHHAENAY
GFARTGQTIVNVPDAKLMKLYVDDEPLLLSINEIQSYKRWIDFREGVLRRELVWRTPAGKLLRVATSRMVSFTHRHMALI
SIEVTMLEGDAPIVISSQILNRQDGMDEYHARPDDIEKTADPRQARKFADKVLIPEKDWHSDRRMILGFRTARSGMTLAV
GADHEIITENEYAALIDTEPGMGKRVYRVEATQGRPIRIDKAVAYHTSRGVPVHELFDRVRRSLDRVREQGHNVYYDEQR
AWLDDYWATADVEVEGAPTGIQQAVRWNLFQIAQASARADQLGIPAKGVTGSGYEGHYFWDTEVYVIPMLTYTHPRIAEN
ALRFRVNTLPQARRRAKELSERGALFPWRTITGEEASAYYAAGTAQYHINADIVHAIMNHARATEDKTFLFRDAAPVLVE
TARMWADLGFWRINGGREFHIHGVTGPDEYTTVVNNNLYTNVMARANLIDAAGVIRRMRDEDPLWYEHLCSELDLTEDEV
GGWEECAAGMVIPFDDTFGIHPQDDQFLSRELWDLKNTPGNKRPLLLHYHPLVIYRFQVLKQADVVLALFLQGDQFTQAV
KLADFEYYDPITTGDSSLSAVVQSVMAAEVGHQEMAMGYFLDALFCDLADTHHNASDGVHVASTGGVWGCLVHGFVGMRN
DRENLRFDPRLPAQWTSIKHHMLIGDSHMLVFLERDAITFQILSGHDRDVTVRGELYHIGVEPVRVPLSDQGPLRPSLRV
THPISGLRRADGSLITAEVPGSIAETIVSSPISTS
>Mature_834_residues
TRTGTDPMDRWRFPDDPWAIVETAPSTDDLGTTETLFSIGNGYLGMRGNPSEGRDSFSHGTYINGFHEIWDIHHAENAYG
FARTGQTIVNVPDAKLMKLYVDDEPLLLSINEIQSYKRWIDFREGVLRRELVWRTPAGKLLRVATSRMVSFTHRHMALIS
IEVTMLEGDAPIVISSQILNRQDGMDEYHARPDDIEKTADPRQARKFADKVLIPEKDWHSDRRMILGFRTARSGMTLAVG
ADHEIITENEYAALIDTEPGMGKRVYRVEATQGRPIRIDKAVAYHTSRGVPVHELFDRVRRSLDRVREQGHNVYYDEQRA
WLDDYWATADVEVEGAPTGIQQAVRWNLFQIAQASARADQLGIPAKGVTGSGYEGHYFWDTEVYVIPMLTYTHPRIAENA
LRFRVNTLPQARRRAKELSERGALFPWRTITGEEASAYYAAGTAQYHINADIVHAIMNHARATEDKTFLFRDAAPVLVET
ARMWADLGFWRINGGREFHIHGVTGPDEYTTVVNNNLYTNVMARANLIDAAGVIRRMRDEDPLWYEHLCSELDLTEDEVG
GWEECAAGMVIPFDDTFGIHPQDDQFLSRELWDLKNTPGNKRPLLLHYHPLVIYRFQVLKQADVVLALFLQGDQFTQAVK
LADFEYYDPITTGDSSLSAVVQSVMAAEVGHQEMAMGYFLDALFCDLADTHHNASDGVHVASTGGVWGCLVHGFVGMRND
RENLRFDPRLPAQWTSIKHHMLIGDSHMLVFLERDAITFQILSGHDRDVTVRGELYHIGVEPVRVPLSDQGPLRPSLRVT
HPISGLRRADGSLITAEVPGSIAETIVSSPISTS

Specific function: Unknown

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=534, Percent_Identity=25.4681647940075, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1787575, Length=791, Percent_Identity=28.6978508217446, Blast_Score=297, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6325283, Length=463, Percent_Identity=20.5183585313175, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583760, Length=318, Percent_Identity=24.2138364779874, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: 2.4.1.64

Molecular weight: Translated: 94289; Mature: 94158

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTGTDPMDRWRFPDDPWAIVETAPSTDDLGTTETLFSIGNGYLGMRGNPSEGRDSFSH
CCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCCC
GTYINGFHEIWDIHHAENAYGFARTGQTIVNVPDAKLMKLYVDDEPLLLSINEIQSYKRW
CCEECCHHHHHHHHCCCCCCCEECCCCEEEECCCCEEEEEEECCCCEEEEHHHHHHHHHH
IDFREGVLRRELVWRTPAGKLLRVATSRMVSFTHRHMALISIEVTMLEGDAPIVISSQIL
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCEEEEHHHH
NRQDGMDEYHARPDDIEKTADPRQARKFADKVLIPEKDWHSDRRMILGFRTARSGMTLAV
CCCCCCHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEE
GADHEIITENEYAALIDTEPGMGKRVYRVEATQGRPIRIDKAVAYHTSRGVPVHELFDRV
CCCCEEEECCCEEEEEECCCCCCCEEEEEECCCCCCEEEECEEEEECCCCCCHHHHHHHH
RRSLDRVREQGHNVYYDEQRAWLDDYWATADVEVEGAPTGIQQAVRWNLFQIAQASARAD
HHHHHHHHHCCCCEEECCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHH
QLGIPAKGVTGSGYEGHYFWDTEVYVIPMLTYTHPRIAENALRFRVNTLPQARRRAKELS
HCCCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCHHHHHHHHEEECCCHHHHHHHHHHH
ERGALFPWRTITGEEASAYYAAGTAQYHINADIVHAIMNHARATEDKTFLFRDAAPVLVE
HCCCCCCEEECCCCCCCEEEEECEEEEEECHHHHHHHHHHHCCCCCCEEEEECCCHHHHH
TARMWADLGFWRINGGREFHIHGVTGPDEYTTVVNNNLYTNVMARANLIDAAGVIRRMRD
HHHHHHHCCEEEECCCCEEEEEECCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHCC
EDPLWYEHLCSELDLTEDEVGGWEECAAGMVIPFDDTFGIHPQDDQFLSRELWDLKNTPG
CCCHHHHHHHHHCCCCHHHCCCHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
NKRPLLLHYHPLVIYRFQVLKQADVVLALFLQGDQFTQAVKLADFEYYDPITTGDSSLSA
CCCCEEEEECHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHH
VVQSVMAAEVGHQEMAMGYFLDALFCDLADTHHNASDGVHVASTGGVWGCLVHGFVGMRN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCC
DRENLRFDPRLPAQWTSIKHHMLIGDSHMLVFLERDAITFQILSGHDRDVTVRGELYHIG
CHHCCEECCCCCCHHHCCCEEEEECCCEEEEEEECCCEEEEEECCCCCCEEEECEEEEEC
VEPVRVPLSDQGPLRPSLRVTHPISGLRRADGSLITAEVPGSIAETIVSSPISTS
CCEEEECCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TRTGTDPMDRWRFPDDPWAIVETAPSTDDLGTTETLFSIGNGYLGMRGNPSEGRDSFSH
CCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCCC
GTYINGFHEIWDIHHAENAYGFARTGQTIVNVPDAKLMKLYVDDEPLLLSINEIQSYKRW
CCEECCHHHHHHHHCCCCCCCEECCCCEEEECCCCEEEEEEECCCCEEEEHHHHHHHHHH
IDFREGVLRRELVWRTPAGKLLRVATSRMVSFTHRHMALISIEVTMLEGDAPIVISSQIL
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCEEEEHHHH
NRQDGMDEYHARPDDIEKTADPRQARKFADKVLIPEKDWHSDRRMILGFRTARSGMTLAV
CCCCCCHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEE
GADHEIITENEYAALIDTEPGMGKRVYRVEATQGRPIRIDKAVAYHTSRGVPVHELFDRV
CCCCEEEECCCEEEEEECCCCCCCEEEEEECCCCCCEEEECEEEEECCCCCCHHHHHHHH
RRSLDRVREQGHNVYYDEQRAWLDDYWATADVEVEGAPTGIQQAVRWNLFQIAQASARAD
HHHHHHHHHCCCCEEECCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHH
QLGIPAKGVTGSGYEGHYFWDTEVYVIPMLTYTHPRIAENALRFRVNTLPQARRRAKELS
HCCCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCHHHHHHHHEEECCCHHHHHHHHHHH
ERGALFPWRTITGEEASAYYAAGTAQYHINADIVHAIMNHARATEDKTFLFRDAAPVLVE
HCCCCCCEEECCCCCCCEEEEECEEEEEECHHHHHHHHHHHCCCCCCEEEEECCCHHHHH
TARMWADLGFWRINGGREFHIHGVTGPDEYTTVVNNNLYTNVMARANLIDAAGVIRRMRD
HHHHHHHCCEEEECCCCEEEEEECCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHCC
EDPLWYEHLCSELDLTEDEVGGWEECAAGMVIPFDDTFGIHPQDDQFLSRELWDLKNTPG
CCCHHHHHHHHHCCCCHHHCCCHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
NKRPLLLHYHPLVIYRFQVLKQADVVLALFLQGDQFTQAVKLADFEYYDPITTGDSSLSA
CCCCEEEEECHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHH
VVQSVMAAEVGHQEMAMGYFLDALFCDLADTHHNASDGVHVASTGGVWGCLVHGFVGMRN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCC
DRENLRFDPRLPAQWTSIKHHMLIGDSHMLVFLERDAITFQILSGHDRDVTVRGELYHIG
CHHCCEECCCCCCHHHCCCEEEEECCCEEEEEEECCCEEEEEECCCCCCEEEECEEEEEC
VEPVRVPLSDQGPLRPSLRVTHPISGLRRADGSLITAEVPGSIAETIVSSPISTS
CCEEEECCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: alpha,alpha-trehalose; phosphate

Specific reaction: alpha,alpha-trehalose + phosphate = D-glucose + beta-D-glucose 1-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]