Click here to switch to the map view.

The map label for this gene is iscS [H]

Identifier: 50842595

GI number: 50842595

Start: 1215939

End: 1217078

Strand: Direct

Name: iscS [H]

Synonym: PPA1116

Alternate gene names: 50842595

Gene position: 1215939-1217078 (Clockwise)

Preceding gene: 50842587

Following gene: 50842596

Centisome position: 47.49

GC content: 62.46

Gene sequence:

>1140_bases
ATGTCGCAGCTGAAGCCCGGATCAGTACACCACGACCGCGCCTACTTCGACCATGCGGCAACATCCCCACTACGCCGCGA
GGCACGTGAGGCAATGGCATGTACGGTACCGGGCAACCCTGGCGCCATTCACGGTTCAGGCCGTGCAGCGCGGGCACTTC
TAGAGGATGCCCGCGAGGAGATCGCCAGCCTGCTAGGGGTCGGTCCGCTTGAGATTGTGTTCACCAGTGGAGGAACCGAG
TCGGACATGCTTGCGGTGATGGGAGCTGTTCGCGACGGCCACGCGCTGGTGTCAGCAGTTGAACATCCAGCCGTCGGCGG
TCTGGTCTCCCCGTGGCTTCTTGGCAAGCGATGTGAATTGCTACCGGTGAACCATGCCGGGCTTGTCGACCCGGATGAGG
TGGGCGGACACTCCGGCAATCTTGACGTTGTTTCCGTCATGGCTGTCAACAACGAGACCGGAGCTTGCCAGCCCGTCTCG
CGGGTCGCTGAGGCGGCCCACCAGGCTGGGGCGCTCATGCATACCGACGCCGTCCAGGCCCTTGGCCACATCCCTGTTGA
CCTCGACGGATGGGGGGTAGACCTCGCTTCCTTCTCCGCTCACAAGATTGGTGGGCCGGTTGGGATTGGAGCACTCTGGG
TGAAACGGGGAGTCGAACTGCACCCCGTTTCCCCAGGGGGAGGTCAGCAGTACGGCATGCGCTCGGGAACCCAGCCGGTG
GCATTGGCCTGCGGCTTCGCCGCAGCTTTACGCGCCTGTGTCGATGAGTTCGACACCATGACGGCCCGGTGGCAGTCGTG
GCGGCGGCGCGTCATCGCGTGGTGTCGTCAACAGCCTGAAGTAACTGTTGACGATGCGCCCATGACTAGCCCGTCGATCA
TTCACCTTAGTGTTGCTGGAGCCCGCGGGACTGATCTTGTCATGCTCGCTGACCGCGATGGCATTGACCTCTCCGCCGGT
TCGGCCTGCCACGCTGGGGTATCGCGCCCCTCCCCAACAATGCTTGCCATGGGGAGAGACGACGATGCTGCGTCCTCGGG
ATTAAGAATTTCGATGGGATATACCACCACCGATGTCGATATCGATCGTTTGCTCGTCACCTTACCCGCAACGATACGCA
AGGCAAGCGGAGCCCGGTAA

Upstream 100 bases:

>100_bases
TTTAATCTGGTTGGTCGTAGCGTCCGTAAGGGCGATGATGCGCGCCTTCCACGTCTTGCTGTTGGGCAGTAGCGTCGAGG
AGTCGCCAGGAAGGACAGGT

Downstream 100 bases:

>100_bases
GTGGGCGTCAACGCTTATCATTGAGGTTCCCCCTATGCGGGGGTCAGATCAAGCCGCCACAACACTACGTCACTGTGAGA
GGACACACTGTGAAGGTTCT

Product: aminotransferase class V, putative cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSQLKPGSVHHDRAYFDHAATSPLRREAREAMACTVPGNPGAIHGSGRAARALLEDAREEIASLLGVGPLEIVFTSGGTE
SDMLAVMGAVRDGHALVSAVEHPAVGGLVSPWLLGKRCELLPVNHAGLVDPDEVGGHSGNLDVVSVMAVNNETGACQPVS
RVAEAAHQAGALMHTDAVQALGHIPVDLDGWGVDLASFSAHKIGGPVGIGALWVKRGVELHPVSPGGGQQYGMRSGTQPV
ALACGFAAALRACVDEFDTMTARWQSWRRRVIAWCRQQPEVTVDDAPMTSPSIIHLSVAGARGTDLVMLADRDGIDLSAG
SACHAGVSRPSPTMLAMGRDDDAASSGLRISMGYTTTDVDIDRLLVTLPATIRKASGAR

Sequences:

>Translated_379_residues
MSQLKPGSVHHDRAYFDHAATSPLRREAREAMACTVPGNPGAIHGSGRAARALLEDAREEIASLLGVGPLEIVFTSGGTE
SDMLAVMGAVRDGHALVSAVEHPAVGGLVSPWLLGKRCELLPVNHAGLVDPDEVGGHSGNLDVVSVMAVNNETGACQPVS
RVAEAAHQAGALMHTDAVQALGHIPVDLDGWGVDLASFSAHKIGGPVGIGALWVKRGVELHPVSPGGGQQYGMRSGTQPV
ALACGFAAALRACVDEFDTMTARWQSWRRRVIAWCRQQPEVTVDDAPMTSPSIIHLSVAGARGTDLVMLADRDGIDLSAG
SACHAGVSRPSPTMLAMGRDDDAASSGLRISMGYTTTDVDIDRLLVTLPATIRKASGAR
>Mature_378_residues
SQLKPGSVHHDRAYFDHAATSPLRREAREAMACTVPGNPGAIHGSGRAARALLEDAREEIASLLGVGPLEIVFTSGGTES
DMLAVMGAVRDGHALVSAVEHPAVGGLVSPWLLGKRCELLPVNHAGLVDPDEVGGHSGNLDVVSVMAVNNETGACQPVSR
VAEAAHQAGALMHTDAVQALGHIPVDLDGWGVDLASFSAHKIGGPVGIGALWVKRGVELHPVSPGGGQQYGMRSGTQPVA
LACGFAAALRACVDEFDTMTARWQSWRRRVIAWCRQQPEVTVDDAPMTSPSIIHLSVAGARGTDLVMLADRDGIDLSAGS
ACHAGVSRPSPTMLAMGRDDDAASSGLRISMGYTTTDVDIDRLLVTLPATIRKASGAR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=363, Percent_Identity=36.3636363636364, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=30.4136253041363, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI48994898, Length=374, Percent_Identity=35.8288770053476, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=28.9672544080605, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787970, Length=392, Percent_Identity=23.9795918367347, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=396, Percent_Identity=34.0909090909091, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=26.219512195122, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319831, Length=365, Percent_Identity=34.2465753424658, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI20129463, Length=366, Percent_Identity=37.1584699453552, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 39478; Mature: 39347

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLKPGSVHHDRAYFDHAATSPLRREAREAMACTVPGNPGAIHGSGRAARALLEDAREE
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHEEEECCCCCCEECCCCHHHHHHHHHHHHH
IASLLGVGPLEIVFTSGGTESDMLAVMGAVRDGHALVSAVEHPAVGGLVSPWLLGKRCEL
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCCCE
LPVNHAGLVDPDEVGGHSGNLDVVSVMAVNNETGACQPVSRVAEAAHQAGALMHTDAVQA
EECCCCCCCCHHHCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
LGHIPVDLDGWGVDLASFSAHKIGGPVGIGALWVKRGVELHPVSPGGGQQYGMRSGTQPV
HCCCCCCCCCCCCCHHHCCHHHCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHH
ALACGFAAALRACVDEFDTMTARWQSWRRRVIAWCRQQPEVTVDDAPMTSPSIIHLSVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEC
ARGTDLVMLADRDGIDLSAGSACHAGVSRPSPTMLAMGRDDDAASSGLRISMGYTTTDVD
CCCCEEEEEECCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCCEEEEECCEECCCC
IDRLLVTLPATIRKASGAR
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQLKPGSVHHDRAYFDHAATSPLRREAREAMACTVPGNPGAIHGSGRAARALLEDAREE
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHEEEECCCCCCEECCCCHHHHHHHHHHHHH
IASLLGVGPLEIVFTSGGTESDMLAVMGAVRDGHALVSAVEHPAVGGLVSPWLLGKRCEL
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCCCE
LPVNHAGLVDPDEVGGHSGNLDVVSVMAVNNETGACQPVSRVAEAAHQAGALMHTDAVQA
EECCCCCCCCHHHCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
LGHIPVDLDGWGVDLASFSAHKIGGPVGIGALWVKRGVELHPVSPGGGQQYGMRSGTQPV
HCCCCCCCCCCCCCHHHCCHHHCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHH
ALACGFAAALRACVDEFDTMTARWQSWRRRVIAWCRQQPEVTVDDAPMTSPSIIHLSVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEC
ARGTDLVMLADRDGIDLSAGSACHAGVSRPSPTMLAMGRDDDAASSGLRISMGYTTTDVD
CCCCEEEEEECCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCCEEEEECCEECCCC
IDRLLVTLPATIRKASGAR
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA