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The map label for this gene is liaS [H]

Identifier: 50842553

GI number: 50842553

Start: 1169261

End: 1170601

Strand: Direct

Name: liaS [H]

Synonym: PPA1073

Alternate gene names: 50842553

Gene position: 1169261-1170601 (Clockwise)

Preceding gene: 50842552

Following gene: 50842554

Centisome position: 45.67

GC content: 60.55

Gene sequence:

>1341_bases
TTGTGCGACGTGCGGATCCGAGTTACAGGGGCCTGGGCGCTACACCTACGTCAGGACCTACACTCGGGGCCGATGAGGAC
AACCACATTAGCCCGGGATGGGCTGTACCGTTGGCGTGGCATGGACGCCGGTCACCGGGGACGTGCGGTGGCTATCGAGG
TGGGGATGCAGGTTGGCATCCAGGTGCTGTTCGCTGGCCTTGTCATCTTTACCCTAGTTATGGCCCTGGTGTTGTCTCCG
CCGCGTTTGGGGCGAATCCTCGGATGGGCGGCCTTACTCATCGTTGTGTATACCGCCCTTATGGGGACCCACGTGATGAG
CCGTTCCCGCCGCGAAATGTGGCGCCCGTTGTTGGTGACAGTCATGGTCGTTGTGTCGATCCTGCTGGCTATTGATTTCC
CTTATGCCGCCTATCTGACGATCCCGCTGTCCTTCGTGTACTTGGATCATCTGCCGCCACTGCAGGCATGTGTTGCGGTC
GTCGTCGGAGCGGTGGCTATCCTGCTTGGGGTCGGGTTGACCGGTGGCTGGAGTGTGGGGGGAATCGTCGGCCCAGTCGC
GGGTGCCGCTGTGGCAGTCGTCGTCGGTCTGTCTCTCAAGGCCATGCAGGCTCAGGCGGCCGAGTTGGAACGTCTCAATG
TGGACTTGCTGGCGGTACAAAAGAGGTTGGCTGACTCCCAGCGTCAGGCCGGTGTACTGGAGGAGAGAGCCAGATTAGCC
CGTGAGATCCATGACACCGTCGCTCAATCTCTGTCAAGCATTGGACTGTTGCTTTCTGCGGTGGAGCGTACTGCCCCTGA
CCACCCCGCCATCGAACAGATTCGACTCGCCCATCTCGCCTCCTCAGACGCCCTTTCAGAGACCCGTGGTCTCATTGCTG
AGTTGGCTCCGCCCACGGTCGTCCATCAGGGACTGTCCGCGGTACTCGAACGGCTTGGGGCCACGACATGGTCAATCGGT
GGGTTGCACGTCGACGTGGACTGCCCGGATACGTCCGATTTACCCATGGAGATCCAGACGGCATTGCTGAGACTATGCCA
GGCAGCGATGTCGAATGTGGTGCGTCACGCCCACGCCCGTCATGCGTCGATCCGGATTGTGCGTCCCAACCCCGGGCATG
TGCTCTTGAGAGTTAGTGACGATGGGGTCGGCATGGATCTGGACGCGCCTGTTCGTGACGGGGCATTTGGGTTGCAAGCT
ATTCGGCAACGGGTTGAGGCCGCGTCGGGCGAGGTTTCTTTAACCTCTAAACCAGGGGAGGGCACCCGCGTTGAAGTCGA
TCTTCCCTGCCGTGCAGCATCACCGCATATTGATCGCCACAGCCAAGGAGAATCGTCATGA

Upstream 100 bases:

>100_bases
GTTGGCTGTCGTCCACTCCATCTTCCCGAGACACCTTGGCATCGGTGTACGACTTGGTCATGCGTCGGTTCTGGCCAGAG
GCGTCTCTCGATTCTTTGGA

Downstream 100 bases:

>100_bases
CGGTGCGCGTCCTCATCGCAGATGACCATCCGGTGGTCCGAGCCGGGGTTACGGCATTGCTAGACACCGATCCCGACATT
GAGATTGTTGCGACCGCAGC

Product: putative two-component system sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MCDVRIRVTGAWALHLRQDLHSGPMRTTTLARDGLYRWRGMDAGHRGRAVAIEVGMQVGIQVLFAGLVIFTLVMALVLSP
PRLGRILGWAALLIVVYTALMGTHVMSRSRREMWRPLLVTVMVVVSILLAIDFPYAAYLTIPLSFVYLDHLPPLQACVAV
VVGAVAILLGVGLTGGWSVGGIVGPVAGAAVAVVVGLSLKAMQAQAAELERLNVDLLAVQKRLADSQRQAGVLEERARLA
REIHDTVAQSLSSIGLLLSAVERTAPDHPAIEQIRLAHLASSDALSETRGLIAELAPPTVVHQGLSAVLERLGATTWSIG
GLHVDVDCPDTSDLPMEIQTALLRLCQAAMSNVVRHAHARHASIRIVRPNPGHVLLRVSDDGVGMDLDAPVRDGAFGLQA
IRQRVEAASGEVSLTSKPGEGTRVEVDLPCRAASPHIDRHSQGESS

Sequences:

>Translated_446_residues
MCDVRIRVTGAWALHLRQDLHSGPMRTTTLARDGLYRWRGMDAGHRGRAVAIEVGMQVGIQVLFAGLVIFTLVMALVLSP
PRLGRILGWAALLIVVYTALMGTHVMSRSRREMWRPLLVTVMVVVSILLAIDFPYAAYLTIPLSFVYLDHLPPLQACVAV
VVGAVAILLGVGLTGGWSVGGIVGPVAGAAVAVVVGLSLKAMQAQAAELERLNVDLLAVQKRLADSQRQAGVLEERARLA
REIHDTVAQSLSSIGLLLSAVERTAPDHPAIEQIRLAHLASSDALSETRGLIAELAPPTVVHQGLSAVLERLGATTWSIG
GLHVDVDCPDTSDLPMEIQTALLRLCQAAMSNVVRHAHARHASIRIVRPNPGHVLLRVSDDGVGMDLDAPVRDGAFGLQA
IRQRVEAASGEVSLTSKPGEGTRVEVDLPCRAASPHIDRHSQGESS
>Mature_446_residues
MCDVRIRVTGAWALHLRQDLHSGPMRTTTLARDGLYRWRGMDAGHRGRAVAIEVGMQVGIQVLFAGLVIFTLVMALVLSP
PRLGRILGWAALLIVVYTALMGTHVMSRSRREMWRPLLVTVMVVVSILLAIDFPYAAYLTIPLSFVYLDHLPPLQACVAV
VVGAVAILLGVGLTGGWSVGGIVGPVAGAAVAVVVGLSLKAMQAQAAELERLNVDLLAVQKRLADSQRQAGVLEERARLA
REIHDTVAQSLSSIGLLLSAVERTAPDHPAIEQIRLAHLASSDALSETRGLIAELAPPTVVHQGLSAVLERLGATTWSIG
GLHVDVDCPDTSDLPMEIQTALLRLCQAAMSNVVRHAHARHASIRIVRPNPGHVLLRVSDDGVGMDLDAPVRDGAFGLQA
IRQRVEAASGEVSLTSKPGEGTRVEVDLPCRAASPHIDRHSQGESS

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=222, Percent_Identity=27.027027027027, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787474, Length=212, Percent_Identity=29.2452830188679, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082311, Length=237, Percent_Identity=28.2700421940928, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47628; Mature: 47628

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]