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The map label for this gene is gyrB [H]

Identifier: 50842524

GI number: 50842524

Start: 1132798

End: 1134894

Strand: Direct

Name: gyrB [H]

Synonym: PPA1042

Alternate gene names: 50842524

Gene position: 1132798-1134894 (Clockwise)

Preceding gene: 50842523

Following gene: 50842525

Centisome position: 44.25

GC content: 59.66

Gene sequence:

>2097_bases
GTGCGCACGGCGTCGACTAGCCGGGAGTACGGCGCCAAGAATTTATTGGTGTTGGAGGGGCTCGAGGCGGTACGCAAGCG
TCCGGCCATGTACATCGGGTCGACGGATACCCGCGGTCTCATGCACTGTCTATGGGAAATCTTCGACAACGGTGTTGATG
AGGCGCTTGCTGGGTATGGACGCCGCATCACCGTCACTTTGGAGAAGGCCGGGTCGATCCTCGTTACTGACGAGGGTCGT
GGCATCCCGGTTGACGTCGAGCCGCGCACCGGACTCAGCGGCCTCGAAGTGGTTTACACCAAACTGCATGCGGGCGGGAA
ATTCGGCGGTGGGGCTTATAACGTCTCAGGTGGGCTGCATGGCGTCGGTGCTTCGGTCGTCAATGCCGTCTCAGTGCGCT
TGGATGTGGAGGTGGATCGCAAGTCCACGGTGTGGGGGATGAGCTTTCGCCGTGGCGTTCCTGGGGTCTTTGAGGGGGAG
GGCCCCGATGCCCCCTTTACTCCTGGATCGGGGGTGCGCAAAGTAGGCAAGGCCAAGCGTGGTGTGACCGGTACTCGCGT
TCGGTACTGGCCCGATCGTCAGATCTTCCTTCCTGATGCCAAGCTGTCATGGTCCAAACTTGCTGACCGTGCTCGTCAGA
CGGCTTTCCTCGTCCCCGGCTTGGAGATCGTCATTACTGACGAGAGGGGTATTCAGACTGATCCTGAGACTGGCGATGTG
CTGGAGACGGTCTCAGAAACGATGCGTTTCGATGGCGGCATCTCTGAGTTCGCTGAGTATCTCGCCACTGACCAGCCTGT
CAACAATGTCATGAGATTGCAGGGGGAGACTTCCTTCACCGAGACCGTCCCCATGCTTGACGAGAACGGTGGTATGACGC
CTACCGACGTCGATCGGGACCTCGGGGTCGACATCGCCCTGCGATGGGGGACCGGTTACGACACTACGGTGCGTTCCTTC
GTCAACATCATCGCTACTCCTAAGGGGGGCACCCACGTTCAGGGGTTCGAACAGGGTCTGTTACGAGCCTTCAGTGCCGG
TCTAGAGGGGACACGCATCCTCAAGTCCTCAGAAGAGATCGTTAAGGATGACGTCCTTGAGGGGATGACGGCCGTGGTGA
CGGTGAGCCTGGCAGAGCCCCAGTTTGAGGGTCAGACCAAGGAGGTGCTGGGTACTCCGCCGGTTCGACGCCTTGTGGCG
CGCATTGTTGAGGACAAGATGACTGCCTTCCTCACTTCGGCCAAGGCGGCTGACAAACCAGTAGCACGCGCCCTTATGGA
GAAGGTCGTTAATGCTTCGCGGACCCGTGTGGCTGCCCGGGCACACAAGGAGAACCAGCGTCGTAAGAATGCTCTGGAAT
CCTCTCACCTGCCGCCCAAGCTCAAGGACTGTCGATCTTCCGATCCGGACGTTACCGAGCTGTTCATCGTCGAGGGTGAT
TCCGCCCTCGGAACGGCCAACGTGGCTCGTAACTCCGAGCACCAGGCTTTGCTTCCGATACGCGGCAAGATTCTCAATGT
GCAGAAGGCTGATCTCGGTGCCATGCTCAAGAACGCCGAGTGCGCCTCGATCATCCAGGTCGTTGGTGCCGGGTCGGGCA
AGACCTTCGACCTCGACCAGGCCCGTTATGGCAAGGTCATCTTCATGGCCGACGCCGACTCCGATGGTGCCCATATTCGT
TGCTTGCTGGCCACCCTGTTCTTCCGTTATATGCGCCCAATGGTGGAGGCTGGCCGGGTGTTCTCGGCGGTACCACCGTT
GCACCGATTCGAACTCATCAACCCTAAACGGGGGATGGACAAGTACCTATACACCTATACCGATGCCGAGTACCAGCGCA
CCGCCGCCCAACTGGCGAAGAAAGGGGTGCGGTTCAAGGAACCGCAGCGGTACAAAGGTCTCGGTGAGATGGACGCTTCC
CAACTCAAGGAGACGACGATGGACCCGCGCCATCGCACTCTGCGTCGCATCACCGTCGACGACGCCGCGGAGGCCGAGGG
CACCTTTGAGATCCTTATGGGCAATGAGGTAGCCCCGCGTAAACAGTTCATCGTCGAGGGGGCTTACCGTCTCGATGCTG
AACAGATCGACGCTTAA

Upstream 100 bases:

>100_bases
CCGTGTCGTCGGCTGTCGATAAGCTGCCACCGTGACCATGGACAACATCTCGACCTCATCAGCCAACAGCTCGGAAACGC
CACGTGGTAAGGGCGATACC

Downstream 100 bases:

>100_bases
GAGGGCTCGTTCGCGTCCCTGCGCGTCAGTGGCCTCGCGGAGACGCGAGGTCTAGCGAGGCGCATGCTCCTCGCTGTCTA
AGTGTTGGCCTAGGGGCGAC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase II subunit B [H]

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MRTASTSREYGAKNLLVLEGLEAVRKRPAMYIGSTDTRGLMHCLWEIFDNGVDEALAGYGRRITVTLEKAGSILVTDEGR
GIPVDVEPRTGLSGLEVVYTKLHAGGKFGGGAYNVSGGLHGVGASVVNAVSVRLDVEVDRKSTVWGMSFRRGVPGVFEGE
GPDAPFTPGSGVRKVGKAKRGVTGTRVRYWPDRQIFLPDAKLSWSKLADRARQTAFLVPGLEIVITDERGIQTDPETGDV
LETVSETMRFDGGISEFAEYLATDQPVNNVMRLQGETSFTETVPMLDENGGMTPTDVDRDLGVDIALRWGTGYDTTVRSF
VNIIATPKGGTHVQGFEQGLLRAFSAGLEGTRILKSSEEIVKDDVLEGMTAVVTVSLAEPQFEGQTKEVLGTPPVRRLVA
RIVEDKMTAFLTSAKAADKPVARALMEKVVNASRTRVAARAHKENQRRKNALESSHLPPKLKDCRSSDPDVTELFIVEGD
SALGTANVARNSEHQALLPIRGKILNVQKADLGAMLKNAECASIIQVVGAGSGKTFDLDQARYGKVIFMADADSDGAHIR
CLLATLFFRYMRPMVEAGRVFSAVPPLHRFELINPKRGMDKYLYTYTDAEYQRTAAQLAKKGVRFKEPQRYKGLGEMDAS
QLKETTMDPRHRTLRRITVDDAAEAEGTFEILMGNEVAPRKQFIVEGAYRLDAEQIDA

Sequences:

>Translated_698_residues
MRTASTSREYGAKNLLVLEGLEAVRKRPAMYIGSTDTRGLMHCLWEIFDNGVDEALAGYGRRITVTLEKAGSILVTDEGR
GIPVDVEPRTGLSGLEVVYTKLHAGGKFGGGAYNVSGGLHGVGASVVNAVSVRLDVEVDRKSTVWGMSFRRGVPGVFEGE
GPDAPFTPGSGVRKVGKAKRGVTGTRVRYWPDRQIFLPDAKLSWSKLADRARQTAFLVPGLEIVITDERGIQTDPETGDV
LETVSETMRFDGGISEFAEYLATDQPVNNVMRLQGETSFTETVPMLDENGGMTPTDVDRDLGVDIALRWGTGYDTTVRSF
VNIIATPKGGTHVQGFEQGLLRAFSAGLEGTRILKSSEEIVKDDVLEGMTAVVTVSLAEPQFEGQTKEVLGTPPVRRLVA
RIVEDKMTAFLTSAKAADKPVARALMEKVVNASRTRVAARAHKENQRRKNALESSHLPPKLKDCRSSDPDVTELFIVEGD
SALGTANVARNSEHQALLPIRGKILNVQKADLGAMLKNAECASIIQVVGAGSGKTFDLDQARYGKVIFMADADSDGAHIR
CLLATLFFRYMRPMVEAGRVFSAVPPLHRFELINPKRGMDKYLYTYTDAEYQRTAAQLAKKGVRFKEPQRYKGLGEMDAS
QLKETTMDPRHRTLRRITVDDAAEAEGTFEILMGNEVAPRKQFIVEGAYRLDAEQIDA
>Mature_698_residues
MRTASTSREYGAKNLLVLEGLEAVRKRPAMYIGSTDTRGLMHCLWEIFDNGVDEALAGYGRRITVTLEKAGSILVTDEGR
GIPVDVEPRTGLSGLEVVYTKLHAGGKFGGGAYNVSGGLHGVGASVVNAVSVRLDVEVDRKSTVWGMSFRRGVPGVFEGE
GPDAPFTPGSGVRKVGKAKRGVTGTRVRYWPDRQIFLPDAKLSWSKLADRARQTAFLVPGLEIVITDERGIQTDPETGDV
LETVSETMRFDGGISEFAEYLATDQPVNNVMRLQGETSFTETVPMLDENGGMTPTDVDRDLGVDIALRWGTGYDTTVRSF
VNIIATPKGGTHVQGFEQGLLRAFSAGLEGTRILKSSEEIVKDDVLEGMTAVVTVSLAEPQFEGQTKEVLGTPPVRRLVA
RIVEDKMTAFLTSAKAADKPVARALMEKVVNASRTRVAARAHKENQRRKNALESSHLPPKLKDCRSSDPDVTELFIVEGD
SALGTANVARNSEHQALLPIRGKILNVQKADLGAMLKNAECASIIQVVGAGSGKTFDLDQARYGKVIFMADADSDGAHIR
CLLATLFFRYMRPMVEAGRVFSAVPPLHRFELINPKRGMDKYLYTYTDAEYQRTAAQLAKKGVRFKEPQRYKGLGEMDAS
QLKETTMDPRHRTLRRITVDDAAEAEGTFEILMGNEVAPRKQFIVEGAYRLDAEQIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=370, Percent_Identity=27.8378378378378, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI19913408, Length=362, Percent_Identity=27.0718232044199, Blast_Score=98, Evalue=3e-20,
Organism=Escherichia coli, GI48994957, Length=607, Percent_Identity=37.7265238879736, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1789408, Length=656, Percent_Identity=37.3475609756098, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=347, Percent_Identity=25.3602305475504, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI212645845, Length=348, Percent_Identity=25.5747126436782, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324241, Length=378, Percent_Identity=26.984126984127, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17136538, Length=702, Percent_Identity=23.6467236467236, Blast_Score=103, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 76306; Mature: 76306

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTASTSREYGAKNLLVLEGLEAVRKRPAMYIGSTDTRGLMHCLWEIFDNGVDEALAGYG
CCCCCCCHHCCCCCEEEHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCHHHHHHCCC
RRITVTLEKAGSILVTDEGRGIPVDVEPRTGLSGLEVVYTKLHAGGKFGGGAYNVSGGLH
CEEEEEEECCCCEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCC
GVGASVVNAVSVRLDVEVDRKSTVWGMSFRRGVPGVFEGEGPDAPFTPGSGVRKVGKAKR
HHHHHHHHEEEEEEEEEECCCCCEECCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GVTGTRVRYWPDRQIFLPDAKLSWSKLADRARQTAFLVPGLEIVITDERGIQTDPETGDV
CCCCCEEEECCCCEEECCCCCCCHHHHHHHHHHCEEECCCEEEEEECCCCCCCCCCCHHH
LETVSETMRFDGGISEFAEYLATDQPVNNVMRLQGETSFTETVPMLDENGGMTPTDVDRD
HHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCCCCCCHHHCCCCEECCCCCCCCCCCCC
LGVDIALRWGTGYDTTVRSFVNIIATPKGGTHVQGFEQGLLRAFSAGLEGTRILKSSEEI
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCHHHH
VKDDVLEGMTAVVTVSLAEPQFEGQTKEVLGTPPVRRLVARIVEDKMTAFLTSAKAADKP
HHHHHHCCCEEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
VARALMEKVVNASRTRVAARAHKENQRRKNALESSHLPPKLKDCRSSDPDVTELFIVEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEECC
SALGTANVARNSEHQALLPIRGKILNVQKADLGAMLKNAECASIIQVVGAGSGKTFDLDQ
CCCCCCHHHCCCCCCEEEECCCEEEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCC
ARYGKVIFMADADSDGAHIRCLLATLFFRYMRPMVEAGRVFSAVPPLHRFELINPKRGMD
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHEEECCCCCCCC
KYLYTYTDAEYQRTAAQLAKKGVRFKEPQRYKGLGEMDASQLKETTMDPRHRTLRRITVD
CEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHCCCHHHHHHHEEECC
DAAEAEGTFEILMGNEVAPRKQFIVEGAYRLDAEQIDA
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MRTASTSREYGAKNLLVLEGLEAVRKRPAMYIGSTDTRGLMHCLWEIFDNGVDEALAGYG
CCCCCCCHHCCCCCEEEHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCHHHHHHCCC
RRITVTLEKAGSILVTDEGRGIPVDVEPRTGLSGLEVVYTKLHAGGKFGGGAYNVSGGLH
CEEEEEEECCCCEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCC
GVGASVVNAVSVRLDVEVDRKSTVWGMSFRRGVPGVFEGEGPDAPFTPGSGVRKVGKAKR
HHHHHHHHEEEEEEEEEECCCCCEECCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GVTGTRVRYWPDRQIFLPDAKLSWSKLADRARQTAFLVPGLEIVITDERGIQTDPETGDV
CCCCCEEEECCCCEEECCCCCCCHHHHHHHHHHCEEECCCEEEEEECCCCCCCCCCCHHH
LETVSETMRFDGGISEFAEYLATDQPVNNVMRLQGETSFTETVPMLDENGGMTPTDVDRD
HHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCCCCCCHHHCCCCEECCCCCCCCCCCCC
LGVDIALRWGTGYDTTVRSFVNIIATPKGGTHVQGFEQGLLRAFSAGLEGTRILKSSEEI
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCHHHH
VKDDVLEGMTAVVTVSLAEPQFEGQTKEVLGTPPVRRLVARIVEDKMTAFLTSAKAADKP
HHHHHHCCCEEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
VARALMEKVVNASRTRVAARAHKENQRRKNALESSHLPPKLKDCRSSDPDVTELFIVEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEECC
SALGTANVARNSEHQALLPIRGKILNVQKADLGAMLKNAECASIIQVVGAGSGKTFDLDQ
CCCCCCHHHCCCCCCEEEECCCEEEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCC
ARYGKVIFMADADSDGAHIRCLLATLFFRYMRPMVEAGRVFSAVPPLHRFELINPKRGMD
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHEEECCCCCCCC
KYLYTYTDAEYQRTAAQLAKKGVRFKEPQRYKGLGEMDASQLKETTMDPRHRTLRRITVD
CEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHCCCHHHHHHHEEECC
DAAEAEGTFEILMGNEVAPRKQFIVEGAYRLDAEQIDA
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8863738; 10360571 [H]