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The map label for this gene is hrdB [H]

Identifier: 50842515

GI number: 50842515

Start: 1122642

End: 1124162

Strand: Direct

Name: hrdB [H]

Synonym: PPA1032

Alternate gene names: 50842515

Gene position: 1122642-1124162 (Clockwise)

Preceding gene: 50842513

Following gene: 50842517

Centisome position: 43.85

GC content: 58.91

Gene sequence:

>1521_bases
GTGTCGACCACCACGTCCAGCACTGCAAACACCGCTGCAGACGAGGAGGCGAAGATAGCCACCGCGAGGAAGAGTACACG
TTCCACCTCTGCTAAGACCGGTACGACCGGTCGGGCGAAGAAGACCACTGCGACGAAGGCAAGCCAGTCGTCGGCTCACA
AGTCCGCTGGTGGGGCCAAGACTCCCACGGCTAAGTCATCTGGGGCCAAAACCACGACGAAGCCCACCAGCACTAAGACG
GCCGCGAAGTCCACCAAGACAGCGGCGAGTAAGACGACGAAGTCTTCGGGTACGAAGACTGTTGGAAACGCGTCGTCCGG
TGGTGCCAAAAAGTCGACAACTAAGAGGCGGACCACTAAGGCGAAGAAGGAAACGACCGTCGCCGAAGCCGCATCCGGTG
AGGTCGATGTGACCGTGGAGCGCGACGGTGACGACGTTGTGCTGACTATTGGCGGCAAGAAGCGCTCCCTCGACGATGTT
GACGAGCGTGACTACGACCCGGCCGAGGCTGCTAAGGACGAGCAGCAGATCAACAAGGAGACCGAGGCGTTCTCCTTGTC
TGATAACGATGACGCTGACGAGCCCGAGCAGACCGTCATGGTCGCCGGCGCTACCGCAGATCCCGTTAAGGATTACCTTA
AGCAGATCGGCAAAGTAGCTCTGCTCAATGCCGTCGAAGAGGTTGATCTCGCCAAGCGCATTGAGGCCGGTCTGTTTGCC
GGCGAGCAGCTAGCCGACCCGGATGCCAAGATCCGGGAGAAGGACCGCGAGGATTACGAGTGGATCGCCGAGGACGGCAA
GGTGGCGAAGAACCACCTATTGGAGGCCAACCTCCGTCTGGTCGTCTCCTTGGCTAAGCGTTACACCGGTCGCGGCATGC
TCTTCCTTGACCTCATTCAGGAGGGCAACCTCGGTCTCATTCGTGCCGTCGAGAAATTCGACTACACCAAGGGCTACAAA
TTCTCTACGTACGCGACGTGGTGGATCAAGCAGGCCATTACCCGCGCCATGGCCGACCAGGCTCGTACTATTCGTATCCC
TGTGCATATGGTCGAGGTCATCAACAAGCTGGCTCGCGTCCAGCGTCAGATGCTCCAGGACCTAGGTCGTGAGCCCACCC
CGGAAGAGCTTGCCAGCGAACTCGATATGACCGCAGAGAAGGTCATTGAGGTGCAGAAATACGGTCGCGAGCCGATCTCG
CTGCATACCCCACTGGGTGAGGATGGCGATTCTGAGTTTGGTGACCTTATTGAGGATTCTGAGGCCATCGTGCCAGCAGA
CGCCGTCAACTTCACCCTGTTGCAGGAGCAGCTGCATGATGTCCTCGATACCTTGTCCGAGCGAGAGGCCGGTGTTGTGT
CGATGCGATTCGGCTTGACCGACGGACAGCCCAAGACCCTGGATGAGATCGGCAAGGTCTACGGTGTTACTCGGGAGCGC
ATCCGCCAGATCGAGTCCAAGACCATGTCGAAACTGCGTCATCCGAGTCGCTCTCAGGTACTGCGCGACTACCTCGACTG
A

Upstream 100 bases:

>100_bases
GTATGCCCTTGGGTCTCATGGGGCGGTGATCGTCGATCTTTATGGGCCCATTTGGAGGGGACAGCACTGAAGGACTGGTC
AGTCCTTGGAGAGGATACCC

Downstream 100 bases:

>100_bases
GGATTTAGCAGATGAACGGGGGGTGCCCACTGGGGCGCCCCCCGTCATGTTGAGCCGGTCAGTCCGACGACGTCATCGGT
CAACGTCGTCGAGGAAGTGG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSTTTSSTANTAADEEAKIATARKSTRSTSAKTGTTGRAKKTTATKASQSSAHKSAGGAKTPTAKSSGAKTTTKPTSTKT
AAKSTKTAASKTTKSSGTKTVGNASSGGAKKSTTKRRTTKAKKETTVAEAASGEVDVTVERDGDDVVLTIGGKKRSLDDV
DERDYDPAEAAKDEQQINKETEAFSLSDNDDADEPEQTVMVAGATADPVKDYLKQIGKVALLNAVEEVDLAKRIEAGLFA
GEQLADPDAKIREKDREDYEWIAEDGKVAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYK
FSTYATWWIKQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELASELDMTAEKVIEVQKYGREPIS
LHTPLGEDGDSEFGDLIEDSEAIVPADAVNFTLLQEQLHDVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRER
IRQIESKTMSKLRHPSRSQVLRDYLD

Sequences:

>Translated_506_residues
MSTTTSSTANTAADEEAKIATARKSTRSTSAKTGTTGRAKKTTATKASQSSAHKSAGGAKTPTAKSSGAKTTTKPTSTKT
AAKSTKTAASKTTKSSGTKTVGNASSGGAKKSTTKRRTTKAKKETTVAEAASGEVDVTVERDGDDVVLTIGGKKRSLDDV
DERDYDPAEAAKDEQQINKETEAFSLSDNDDADEPEQTVMVAGATADPVKDYLKQIGKVALLNAVEEVDLAKRIEAGLFA
GEQLADPDAKIREKDREDYEWIAEDGKVAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYK
FSTYATWWIKQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELASELDMTAEKVIEVQKYGREPIS
LHTPLGEDGDSEFGDLIEDSEAIVPADAVNFTLLQEQLHDVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRER
IRQIESKTMSKLRHPSRSQVLRDYLD
>Mature_505_residues
STTTSSTANTAADEEAKIATARKSTRSTSAKTGTTGRAKKTTATKASQSSAHKSAGGAKTPTAKSSGAKTTTKPTSTKTA
AKSTKTAASKTTKSSGTKTVGNASSGGAKKSTTKRRTTKAKKETTVAEAASGEVDVTVERDGDDVVLTIGGKKRSLDDVD
ERDYDPAEAAKDEQQINKETEAFSLSDNDDADEPEQTVMVAGATADPVKDYLKQIGKVALLNAVEEVDLAKRIEAGLFAG
EQLADPDAKIREKDREDYEWIAEDGKVAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKF
STYATWWIKQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELASELDMTAEKVIEVQKYGREPISL
HTPLGEDGDSEFGDLIEDSEAIVPADAVNFTLLQEQLHDVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERI
RQIESKTMSKLRHPSRSQVLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=61.7647058823529, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1789098, Length=319, Percent_Identity=38.2445141065831, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1789871, Length=239, Percent_Identity=30.9623430962343, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=29, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 55082; Mature: 54951

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTSSTANTAADEEAKIATARKSTRSTSAKTGTTGRAKKTTATKASQSSAHKSAGGAK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
TPTAKSSGAKTTTKPTSTKTAAKSTKTAASKTTKSSGTKTVGNASSGGAKKSTTKRRTTK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
AKKETTVAEAASGEVDVTVERDGDDVVLTIGGKKRSLDDVDERDYDPAEAAKDEQQINKE
HHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCH
TEAFSLSDNDDADEPEQTVMVAGATADPVKDYLKQIGKVALLNAVEEVDLAKRIEAGLFA
HHEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEQLADPDAKIREKDREDYEWIAEDGKVAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQ
CCHHCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
EGNLGLIRAVEKFDYTKGYKFSTYATWWIKQAITRAMADQARTIRIPVHMVEVINKLARV
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHH
QRQMLQDLGREPTPEELASELDMTAEKVIEVQKYGREPISLHTPLGEDGDSEFGDLIEDS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHCCC
EAIVPADAVNFTLLQEQLHDVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRER
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IRQIESKTMSKLRHPSRSQVLRDYLD
HHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
STTTSSTANTAADEEAKIATARKSTRSTSAKTGTTGRAKKTTATKASQSSAHKSAGGAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
TPTAKSSGAKTTTKPTSTKTAAKSTKTAASKTTKSSGTKTVGNASSGGAKKSTTKRRTTK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
AKKETTVAEAASGEVDVTVERDGDDVVLTIGGKKRSLDDVDERDYDPAEAAKDEQQINKE
HHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCH
TEAFSLSDNDDADEPEQTVMVAGATADPVKDYLKQIGKVALLNAVEEVDLAKRIEAGLFA
HHEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEQLADPDAKIREKDREDYEWIAEDGKVAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQ
CCHHCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
EGNLGLIRAVEKFDYTKGYKFSTYATWWIKQAITRAMADQARTIRIPVHMVEVINKLARV
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHH
QRQMLQDLGREPTPEELASELDMTAEKVIEVQKYGREPISLHTPLGEDGDSEFGDLIEDS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHCCC
EAIVPADAVNFTLLQEQLHDVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRER
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IRQIESKTMSKLRHPSRSQVLRDYLD
HHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8690707 [H]