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The map label for this gene is pyrE

Identifier: 50842487

GI number: 50842487

Start: 1088644

End: 1089294

Strand: Direct

Name: pyrE

Synonym: PPA1004

Alternate gene names: 50842487

Gene position: 1088644-1089294 (Clockwise)

Preceding gene: 50842486

Following gene: 50842489

Centisome position: 42.52

GC content: 60.98

Gene sequence:

>651_bases
ATGACCGTGACCAATAACGAGATCGCCCGCGAGTTTGCTGAGCGCCTGCTAGCCATCGAGGCCGTGTCGCTGTCTCCGGA
TGCCCCGTTCACTTGGGCATCCGGGCTACACTCGCCCATCTACTGCGACAATCGGGTGACCCTCTCGGATCCGCAGACTC
GTGATCTCATTGCTGATGGGCTGGCTTCACTTGTTCGCACCAATTTCCATCAGGTCGACGTCGTGGCCGGCACTGCTACT
GCCGGCATTGCCCATGCTGCTCTTGCCGCCGATCGGCTTGGCGCACCCATGGCCTATGTGCGCTCTGTTCCAAAGGACCA
TGGGCGGGGCAACCAGATTGAAGGGCGTATTCCAGCTCATTCGAAGGTCATCATGGTCGAGGACCTCATCTCCACAGGTG
GCTCGGTGCTCAAAGCCGCCCAGGCGGTAGAGCGTGAGGGATCGACGGTAGTCGGCGTCCTCGCTCTCTTCTCCTACGAG
CTGGAGAAGGGACACCGCGCCTTCGAGAAGGCGGGAGTGCCGCTGTATACCCTGTCGAATTATCCGATCCTGATCGAGGT
GGCTGCTGAGTCTGGGCGGATCACATCTGAGCAGCAGGCGACGTTGGCGACGTGGTCGTCGGATCCACAAGCCTGGTCGG
ACGCGCACTGA

Upstream 100 bases:

>100_bases
TACCAAAGCTGACGACCCGGTAGCCGCTTATCACGCTATTCGCGATGACTGGAATGCCGGGCGTAAGGCCTGACGACAAC
ACGACCTGGAGGAAATCGCA

Downstream 100 bases:

>100_bases
TGAGCCCGCTGGCGTTCTTGACCCGCTGACTGAGTCTCGTCTCCGGGTGGCAAACCCTCGCGATCTCGTTGGCAAATCCG
CGTGGGTTCCTGGAGCAGGT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTVTNNEIAREFAERLLAIEAVSLSPDAPFTWASGLHSPIYCDNRVTLSDPQTRDLIADGLASLVRTNFHQVDVVAGTAT
AGIAHAALAADRLGAPMAYVRSVPKDHGRGNQIEGRIPAHSKVIMVEDLISTGGSVLKAAQAVEREGSTVVGVLALFSYE
LEKGHRAFEKAGVPLYTLSNYPILIEVAAESGRITSEQQATLATWSSDPQAWSDAH

Sequences:

>Translated_216_residues
MTVTNNEIAREFAERLLAIEAVSLSPDAPFTWASGLHSPIYCDNRVTLSDPQTRDLIADGLASLVRTNFHQVDVVAGTAT
AGIAHAALAADRLGAPMAYVRSVPKDHGRGNQIEGRIPAHSKVIMVEDLISTGGSVLKAAQAVEREGSTVVGVLALFSYE
LEKGHRAFEKAGVPLYTLSNYPILIEVAAESGRITSEQQATLATWSSDPQAWSDAH
>Mature_215_residues
TVTNNEIAREFAERLLAIEAVSLSPDAPFTWASGLHSPIYCDNRVTLSDPQTRDLIADGLASLVRTNFHQVDVVAGTATA
GIAHAALAADRLGAPMAYVRSVPKDHGRGNQIEGRIPAHSKVIMVEDLISTGGSVLKAAQAVEREGSTVVGVLALFSYEL
EKGHRAFEKAGVPLYTLSNYPILIEVAAESGRITSEQQATLATWSSDPQAWSDAH

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=168, Percent_Identity=26.1904761904762, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=195, Percent_Identity=27.6923076923077, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17508631, Length=147, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17933654, Length=215, Percent_Identity=25.5813953488372, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_PROAC (Q6A910)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055714.1
- ProteinModelPortal:   Q6A910
- SMR:   Q6A910
- GeneID:   2932306
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1004
- NMPDR:   fig|267747.1.peg.991
- HOGENOM:   HBG404341
- OMA:   LPMTYVR
- ProtClustDB:   CLSK2763663
- BioCyc:   PACN267747:PPA1004-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23061; Mature: 22930

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 101-101 BINDING 105-105 BINDING 107-107 BINDING 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTNNEIAREFAERLLAIEAVSLSPDAPFTWASGLHSPIYCDNRVTLSDPQTRDLIADG
CCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHCCCCCCEEECCEEEECCCCHHHHHHHH
LASLVRTNFHQVDVVAGTATAGIAHAALAADRLGAPMAYVRSVPKDHGRGNQIEGRIPAH
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCC
SKVIMVEDLISTGGSVLKAAQAVEREGSTVVGVLALFSYELEKGHRAFEKAGVPLYTLSN
CEEEEHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
YPILIEVAAESGRITSEQQATLATWSSDPQAWSDAH
CCEEEEEECCCCCCCCCCHHEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TVTNNEIAREFAERLLAIEAVSLSPDAPFTWASGLHSPIYCDNRVTLSDPQTRDLIADG
CCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHCCCCCCEEECCEEEECCCCHHHHHHHH
LASLVRTNFHQVDVVAGTATAGIAHAALAADRLGAPMAYVRSVPKDHGRGNQIEGRIPAH
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCC
SKVIMVEDLISTGGSVLKAAQAVEREGSTVVGVLALFSYELEKGHRAFEKAGVPLYTLSN
CEEEEHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
YPILIEVAAESGRITSEQQATLATWSSDPQAWSDAH
CCEEEEEECCCCCCCCCCHHEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA