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The map label for this gene is pyrF

Identifier: 50842486

GI number: 50842486

Start: 1087906

End: 1088616

Strand: Direct

Name: pyrF

Synonym: PPA1003

Alternate gene names: 50842486

Gene position: 1087906-1088616 (Clockwise)

Preceding gene: 50842485

Following gene: 50842487

Centisome position: 42.49

GC content: 61.6

Gene sequence:

>711_bases
ATGGACATCACCCGCCCGATTATCGCTCTCGATCTTCCTAGCGCCGAGGCAGCTCTTGACTTCGTAGGTCGATTCGACGG
GGAGAATCTTTTCGTCAAAGTCGGCATGGAGTTGTTTTACGCAGCGGGGCCTAGTGTGGTGACCAGCCTCAAGGAGGCCG
GGCACGACGTCTTCTGTGACCTCAAGCTTCACGACATTCCCAATACCGTCAAGGGTGCCGCTTCTAGCCTCTCCAGGCTG
GGCGCGGACCTACTCACCGTCCATGCAGCCGGTGGTAGGAGCATGATGGAGGCTTCGCTTGAGGGCCTGGGAGATGCCGC
CGAGACCACTCGTGTCATCGCCATTACTCAACTCACCTCCACCTCCCCGGAGGCGTTGAAAACTGAGCAGCTAGTGGATG
TGCCGCTTGTCGAGTCGGTGCGCAACTACGCCAAGCTCGCCCAGGCTGCTGGACTGGCCGGAGTCGTCTGTTCGGCTCAC
GAGGCCGCCGATATCGCCTCGGTAACCGGTCCAAACTTCCTGCGCGTCACGCCGGGAATTCGTCCGGCAGGGTCCGCAGT
AGGGGATCAGTCCAGGGTCGCCACTCCGGGCAACGCCGCCTCGATGGGGTCTTCGGCGATCGTGGTCGGGCGGCCCATTA
CCAAAGCTGACGACCCGGTAGCCGCTTATCACGCTATTCGCGATGACTGGAATGCCGGGCGTAAGGCCTGA

Upstream 100 bases:

>100_bases
CTTGAGCCTCTCATGGACGACTTAAGCATTGCCAGCCTTGAGGACCTTCGGACTCAGGTTAGGCAGTCTCGTTGATCCGA
CCACCACTCAAGGAGTTCAT

Downstream 100 bases:

>100_bases
CGACAACACGACCTGGAGGAAATCGCAATGACCGTGACCAATAACGAGATCGCCCGCGAGTTTGCTGAGCGCCTGCTAGC
CATCGAGGCCGTGTCGCTGT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MDITRPIIALDLPSAEAALDFVGRFDGENLFVKVGMELFYAAGPSVVTSLKEAGHDVFCDLKLHDIPNTVKGAASSLSRL
GADLLTVHAAGGRSMMEASLEGLGDAAETTRVIAITQLTSTSPEALKTEQLVDVPLVESVRNYAKLAQAAGLAGVVCSAH
EAADIASVTGPNFLRVTPGIRPAGSAVGDQSRVATPGNAASMGSSAIVVGRPITKADDPVAAYHAIRDDWNAGRKA

Sequences:

>Translated_236_residues
MDITRPIIALDLPSAEAALDFVGRFDGENLFVKVGMELFYAAGPSVVTSLKEAGHDVFCDLKLHDIPNTVKGAASSLSRL
GADLLTVHAAGGRSMMEASLEGLGDAAETTRVIAITQLTSTSPEALKTEQLVDVPLVESVRNYAKLAQAAGLAGVVCSAH
EAADIASVTGPNFLRVTPGIRPAGSAVGDQSRVATPGNAASMGSSAIVVGRPITKADDPVAAYHAIRDDWNAGRKA
>Mature_236_residues
MDITRPIIALDLPSAEAALDFVGRFDGENLFVKVGMELFYAAGPSVVTSLKEAGHDVFCDLKLHDIPNTVKGAASSLSRL
GADLLTVHAAGGRSMMEASLEGLGDAAETTRVIAITQLTSTSPEALKTEQLVDVPLVESVRNYAKLAQAAGLAGVVCSAH
EAADIASVTGPNFLRVTPGIRPAGSAVGDQSRVATPGNAASMGSSAIVVGRPITKADDPVAAYHAIRDDWNAGRKA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=225, Percent_Identity=42.6666666666667, Blast_Score=164, Evalue=5e-42,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_PROAC (Q6A911)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055713.1
- ProteinModelPortal:   Q6A911
- SMR:   Q6A911
- GeneID:   2932702
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1003
- NMPDR:   fig|267747.1.peg.990
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK2763662
- BioCyc:   PACN267747:PPA1003-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24333; Mature: 24333

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 11-11 BINDING 33-33 BINDING 119-119 BINDING 181-181 BINDING 190-190 BINDING 210-210 BINDING 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITRPIIALDLPSAEAALDFVGRFDGENLFVKVGMELFYAAGPSVVTSLKEAGHDVFCD
CCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHHHCCHHHHHHHHHCCCEEEEE
LKLHDIPNTVKGAASSLSRLGADLLTVHAAGGRSMMEASLEGLGDAAETTRVIAITQLTS
EEECCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHCEEEEEEEEECC
TSPEALKTEQLVDVPLVESVRNYAKLAQAAGLAGVVCSAHEAADIASVTGPNFLRVTPGI
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCCCCEEEECCCC
RPAGSAVGDQSRVATPGNAASMGSSAIVVGRPITKADDPVAAYHAIRDDWNAGRKA
CCCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDITRPIIALDLPSAEAALDFVGRFDGENLFVKVGMELFYAAGPSVVTSLKEAGHDVFCD
CCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHHHCCHHHHHHHHHCCCEEEEE
LKLHDIPNTVKGAASSLSRLGADLLTVHAAGGRSMMEASLEGLGDAAETTRVIAITQLTS
EEECCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHCEEEEEEEEECC
TSPEALKTEQLVDVPLVESVRNYAKLAQAAGLAGVVCSAHEAADIASVTGPNFLRVTPGI
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCCCCEEEECCCC
RPAGSAVGDQSRVATPGNAASMGSSAIVVGRPITKADDPVAAYHAIRDDWNAGRKA
CCCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA