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The map label for this gene is otsA [H]

Identifier: 50842445

GI number: 50842445

Start: 1044422

End: 1045825

Strand: Reverse

Name: otsA [H]

Synonym: PPA0961

Alternate gene names: 50842445

Gene position: 1045825-1044422 (Counterclockwise)

Preceding gene: 50842448

Following gene: 50842444

Centisome position: 40.85

GC content: 59.69

Gene sequence:

>1404_bases
ATGTCGGAGACCGTCCAGGACAAGGTCGACTTCCTTGTCATTGCAAACCGCTTACCCGTAGACCGCAAGGTCGACGACGA
CGGCAGCGTTAGTTGGAAGTCCTCCCCCGGCGGCCTCGTCACCGCTTTGGAGCCTGTCATGCAACGCAAGGGCGGCGCAT
GGATCGGATGGCATGGCGCGCCTGACGTAGTCGTCAAACCCTTCCACCATGACGGTTATGACATCCACCCGGTGCCACTG
AGCGCCCAGGAGGTCGAGGAGTACTACGAGGGGTTCTCTAACGCGACCCTGTGGCCGCTGTACCACGACTGCATTGTCGA
GCCAGTCTTCCACCGTGAGTGGTGGGACGCATTCCAGAAGGTCAACAAGAGGTTTGCCGAGCAAGCTGCTGAACAGGCCG
CAGAGGGCGCCACCGTGTGGGTGCAGGATTACCAGCTCAACCTCGTACCGAAGTACCTGCGTGAGATGCGACCAGATCTG
CGCATTGGATTCTTCCTTCACATTCCGTTCCCGCCGATTGAGCTTTACAGCCGTCTGCCGTGGCGCGAAGAGCTCGTCGA
GGGCTTGCTGGGAGCTGACCTCATCGGGTTCCAGACTCCCGGAGCTGCTGCCAATTTCCAGCGCTTGGCAAGGCACCGCC
CGGGGGTCACTGCCGCTCGTGGTCGCGCTCATACCCCGGATGGCCGCACCGTCGTCATCCGTGACTTCCCGATCTCGATT
GACTCACGTGGTTTCCATGAACTGGCCACCTCAGAGAAAGTCAAGGCTGAGGCTGCCAAGCTTCGCGAGGACCTAGGTCA
CCCAGGAACGATCATCTTCGGCGTCGACCGTCTCGACTACACCAAGGGTCTTCGCCAACGTATCCGTGCCGTCGGAGAGC
TGTTCAAAGAGGGCAAGCTCGATCCCCACGAGGTCGTCTTCCTACAGCTAGCCACCCCGTCGCGGGAACGCGTCGAGGAA
TACAAGATCCTGCGCGACGACATCAACCTGCTCGTCGGCCAGATCAATTCGAAGGTCGGGTCGATCGCCAACCGCGCGCT
GGTCTACCGCAATGAGTCAGTTCCGCGTGAAGTGCTAGCAGCGATGTACCAGATGGCTGATCTTATGCTCGTCACCCCGG
TACGTGACGGCATGAACCTGGTCGCCAAAGAGTACATTGCCTGCCGCTCGAACGACGACTCCGCGCTGGTACTTTCCGAG
TTCGCTGGTGCGGCCCGTGAGCTCAAGCAGGCCTACATGGTGAACCCGTATGACATTGACGGTATGAAGGCCACCATCAT
GAAAGCGCTTACGGACTCCCCCAAGATCAAGGCCCGCAAGATGCGTTCCATGCGTCGTCAAGTCTTCGACAAGACGATCG
ATGTCTGGGCAGACAACTTCCTCACCGAGCTAGAAGGCCAGTGA

Upstream 100 bases:

>100_bases
CGCCAAATAGCAAAGAATAACCTCGAATGGGAACCAACGGTTGGTAGCGTGAAAGTGGCCTTGCTGCACAGCATGACCGC
CATGAGAGGTGTGATCAAAG

Downstream 100 bases:

>100_bases
GGGCACATGGCTGGACCACCAGATGAGAAAGGACGAGACCGTTGAAACTGAGCGCCTTGGAGCAGATTCCGCTGTTTGTC
GGACATGACGTCACCCAAAC

Product: alpha,alpha-trehalose-phosphate synthase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSETVQDKVDFLVIANRLPVDRKVDDDGSVSWKSSPGGLVTALEPVMQRKGGAWIGWHGAPDVVVKPFHHDGYDIHPVPL
SAQEVEEYYEGFSNATLWPLYHDCIVEPVFHREWWDAFQKVNKRFAEQAAEQAAEGATVWVQDYQLNLVPKYLREMRPDL
RIGFFLHIPFPPIELYSRLPWREELVEGLLGADLIGFQTPGAAANFQRLARHRPGVTAARGRAHTPDGRTVVIRDFPISI
DSRGFHELATSEKVKAEAAKLREDLGHPGTIIFGVDRLDYTKGLRQRIRAVGELFKEGKLDPHEVVFLQLATPSRERVEE
YKILRDDINLLVGQINSKVGSIANRALVYRNESVPREVLAAMYQMADLMLVTPVRDGMNLVAKEYIACRSNDDSALVLSE
FAGAARELKQAYMVNPYDIDGMKATIMKALTDSPKIKARKMRSMRRQVFDKTIDVWADNFLTELEGQ

Sequences:

>Translated_467_residues
MSETVQDKVDFLVIANRLPVDRKVDDDGSVSWKSSPGGLVTALEPVMQRKGGAWIGWHGAPDVVVKPFHHDGYDIHPVPL
SAQEVEEYYEGFSNATLWPLYHDCIVEPVFHREWWDAFQKVNKRFAEQAAEQAAEGATVWVQDYQLNLVPKYLREMRPDL
RIGFFLHIPFPPIELYSRLPWREELVEGLLGADLIGFQTPGAAANFQRLARHRPGVTAARGRAHTPDGRTVVIRDFPISI
DSRGFHELATSEKVKAEAAKLREDLGHPGTIIFGVDRLDYTKGLRQRIRAVGELFKEGKLDPHEVVFLQLATPSRERVEE
YKILRDDINLLVGQINSKVGSIANRALVYRNESVPREVLAAMYQMADLMLVTPVRDGMNLVAKEYIACRSNDDSALVLSE
FAGAARELKQAYMVNPYDIDGMKATIMKALTDSPKIKARKMRSMRRQVFDKTIDVWADNFLTELEGQ
>Mature_466_residues
SETVQDKVDFLVIANRLPVDRKVDDDGSVSWKSSPGGLVTALEPVMQRKGGAWIGWHGAPDVVVKPFHHDGYDIHPVPLS
AQEVEEYYEGFSNATLWPLYHDCIVEPVFHREWWDAFQKVNKRFAEQAAEQAAEGATVWVQDYQLNLVPKYLREMRPDLR
IGFFLHIPFPPIELYSRLPWREELVEGLLGADLIGFQTPGAAANFQRLARHRPGVTAARGRAHTPDGRTVVIRDFPISID
SRGFHELATSEKVKAEAAKLREDLGHPGTIIFGVDRLDYTKGLRQRIRAVGELFKEGKLDPHEVVFLQLATPSRERVEEY
KILRDDINLLVGQINSKVGSIANRALVYRNESVPREVLAAMYQMADLMLVTPVRDGMNLVAKEYIACRSNDDSALVLSEF
AGAARELKQAYMVNPYDIDGMKATIMKALTDSPKIKARKMRSMRRQVFDKTIDVWADNFLTELEGQ

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=460, Percent_Identity=33.695652173913, Blast_Score=244, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI115533558, Length=432, Percent_Identity=29.3981481481481, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI115533560, Length=432, Percent_Identity=29.3981481481481, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71987012, Length=432, Percent_Identity=29.3981481481481, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319602, Length=467, Percent_Identity=36.6167023554604, Blast_Score=285, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6323537, Length=402, Percent_Identity=28.1094527363184, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6320279, Length=465, Percent_Identity=27.741935483871, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323917, Length=451, Percent_Identity=27.0509977827051, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI19920676, Length=488, Percent_Identity=34.0163934426229, Blast_Score=269, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 52851; Mature: 52720

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETVQDKVDFLVIANRLPVDRKVDDDGSVSWKSSPGGLVTALEPVMQRKGGAWIGWHGA
CCCCHHHHHCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEECCC
PDVVVKPFHHDGYDIHPVPLSAQEVEEYYEGFSNATLWPLYHDCIVEPVFHREWWDAFQK
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECHHHHHHHHCHHHHHHHHHHHHH
VNKRFAEQAAEQAAEGATVWVQDYQLNLVPKYLREMRPDLRIGFFLHIPFPPIELYSRLP
HHHHHHHHHHHHHHCCCEEEEECCEECCHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCC
WREELVEGLLGADLIGFQTPGAAANFQRLARHRPGVTAARGRAHTPDGRTVVIRDFPISI
CHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCC
DSRGFHELATSEKVKAEAAKLREDLGHPGTIIFGVDRLDYTKGLRQRIRAVGELFKEGKL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
DPHEVVFLQLATPSRERVEEYKILRDDINLLVGQINSKVGSIANRALVYRNESVPREVLA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHH
AMYQMADLMLVTPVRDGMNLVAKEYIACRSNDDSALVLSEFAGAARELKQAYMVNPYDID
HHHHHHHHEEEECCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCC
GMKATIMKALTDSPKIKARKMRSMRRQVFDKTIDVWADNFLTELEGQ
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SETVQDKVDFLVIANRLPVDRKVDDDGSVSWKSSPGGLVTALEPVMQRKGGAWIGWHGA
CCCHHHHHCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEECCC
PDVVVKPFHHDGYDIHPVPLSAQEVEEYYEGFSNATLWPLYHDCIVEPVFHREWWDAFQK
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECHHHHHHHHCHHHHHHHHHHHHH
VNKRFAEQAAEQAAEGATVWVQDYQLNLVPKYLREMRPDLRIGFFLHIPFPPIELYSRLP
HHHHHHHHHHHHHHCCCEEEEECCEECCHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCC
WREELVEGLLGADLIGFQTPGAAANFQRLARHRPGVTAARGRAHTPDGRTVVIRDFPISI
CHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCC
DSRGFHELATSEKVKAEAAKLREDLGHPGTIIFGVDRLDYTKGLRQRIRAVGELFKEGKL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
DPHEVVFLQLATPSRERVEEYKILRDDINLLVGQINSKVGSIANRALVYRNESVPREVLA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHH
AMYQMADLMLVTPVRDGMNLVAKEYIACRSNDDSALVLSEFAGAARELKQAYMVNPYDID
HHHHHHHHEEEECCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCC
GMKATIMKALTDSPKIKARKMRSMRRQVFDKTIDVWADNFLTELEGQ
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA