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The map label for this gene is pdxR [H]

Identifier: 50842448

GI number: 50842448

Start: 1048386

End: 1049798

Strand: Reverse

Name: pdxR [H]

Synonym: PPA0964

Alternate gene names: 50842448

Gene position: 1049798-1048386 (Counterclockwise)

Preceding gene: 50842454

Following gene: 50842445

Centisome position: 41.0

GC content: 64.05

Gene sequence:

>1413_bases
ATGAGACCAGACCTCGTCGTCGACCTGCCGCTACACCTGCCTGACGATCCGCGACCGTTACCGGTCCGGATCTGCGAAGA
AGTGCGTGGACTCATTATGGACGGCGTACTCGCTCCCGGGGATCACCTACCAAGCACCCGAGTCCTGTCAGCACAACTCA
GGGTGTCACGGGGAACCGTCGTCGCCTCTTACGAACAGCTTGAAGCGGAGGGCTATCTCGTGGCCGCCGCCGGCTCAGGG
ACCCAGATCAATCCCGACCTGCGTGGCCTTCACCGTCGCTCTGCCACCACGGCATCCGTCCCCCGACCTCAACGCCATCG
GGGAATCGACCTCACCCCTGGAGTCCCGGACACCGCTGGACTGGCGAACTCGGCATGGCGCTCGGCTTGGCATCGAAGTT
GCGTCAACCCGTCCCGTATCACCGACCCGCTGGGCTCACCGCACCTGCGCCACGAAATTGCCGAACATCTGCGTCAGATG
CGCAGCCTACTGGCCGATCCGCGTCAGGTCGTCGTCACTGGAGGCGCCCGAGCAGGGCTGGCAGAGCTCATCCAGGTGCT
GTGTGATGGGTCCCGCCGCCTGACGATCGGTGTGGAAAGCCCGGGATACCCCAGCCTGAGGAGGGTACCGACGGCCCTCG
GGCATCGGATCGTCGGGCTGCCGACCGACGACCAAGGTCTGGACCCTGCCAGCCTCCCCACCGGACGTGATAGGAAGCGC
CTAGACGCTGTGCTCATCACCCCGAGCCACCAGTACCCGTGGGGCGGGTCACTGTCAGCAAGCCGCCGAGCAGCCGTCAT
CGAGTGGGCGGAGATGGCCTCATGCTGGATTGTCGAAGACGACTTCGATTCTGAACTGCGTCATGTAGGCGCGCCTCTGC
CCGCCCTGGCCTCCCTAGACGAAGAGCACACCATCCTGTTAGGAACCTTCTCCTCCGTGCTCACCCCAGCGCTCGGGTGT
GGCTATCTTGTCGTACCTCCTGATCTCGTCCATTCCTTCGCTCGGCAGCGTCGAGCTACCGGCCAACCCGTTCCAGCCAT
CGTCCAAGAAGCCGTGGCGGATTACCTCTCCACTGGAGCGCTGCGCCGGCGCACCCAACGAATGCGTCAGGTCTACCGAC
GTCGACGCCGCACCGTCATCGACACCTTGGGATCGTTACCGGGGACAACCCTGCAACCGATCGACGGCGGGCTGCACGCT
GTCCTGGTATTTGATGGGTCCGAGGATGACGTCGTCCAGCGATGTCGGCAGGAGGGAGTCGGCGTCACACCATTGTCAGC
ATACTGGGGATCTAGCGACGCCGGAGTGGCAGGGATCGTCCTAGGTTTCGGCAGCCACGACGACGCGACCCTTGCTTCCG
CGCTGAAGATCATCGCCGGTGTCGTAACCACGCATGGCCGCTTATCGTCGTAA

Upstream 100 bases:

>100_bases
GGGATGTGTTCACTCATGGACCCCATGAAATCCACGATTGTGGTTCATGACAAGATCCATATATGACCACTTTCGTTAGA
CCACTTTTCGGTCCACCGTC

Downstream 100 bases:

>100_bases
CAGGGTCATGCCATGGCCGGCACCCCAAACGATGCCGGTCATATACCTCACGAATATGTCCTCAATCGAGGAGGCCTTCG
AAGAGAGCGGTCGACAGGTA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRPDLVVDLPLHLPDDPRPLPVRICEEVRGLIMDGVLAPGDHLPSTRVLSAQLRVSRGTVVASYEQLEAEGYLVAAAGSG
TQINPDLRGLHRRSATTASVPRPQRHRGIDLTPGVPDTAGLANSAWRSAWHRSCVNPSRITDPLGSPHLRHEIAEHLRQM
RSLLADPRQVVVTGGARAGLAELIQVLCDGSRRLTIGVESPGYPSLRRVPTALGHRIVGLPTDDQGLDPASLPTGRDRKR
LDAVLITPSHQYPWGGSLSASRRAAVIEWAEMASCWIVEDDFDSELRHVGAPLPALASLDEEHTILLGTFSSVLTPALGC
GYLVVPPDLVHSFARQRRATGQPVPAIVQEAVADYLSTGALRRRTQRMRQVYRRRRRTVIDTLGSLPGTTLQPIDGGLHA
VLVFDGSEDDVVQRCRQEGVGVTPLSAYWGSSDAGVAGIVLGFGSHDDATLASALKIIAGVVTTHGRLSS

Sequences:

>Translated_470_residues
MRPDLVVDLPLHLPDDPRPLPVRICEEVRGLIMDGVLAPGDHLPSTRVLSAQLRVSRGTVVASYEQLEAEGYLVAAAGSG
TQINPDLRGLHRRSATTASVPRPQRHRGIDLTPGVPDTAGLANSAWRSAWHRSCVNPSRITDPLGSPHLRHEIAEHLRQM
RSLLADPRQVVVTGGARAGLAELIQVLCDGSRRLTIGVESPGYPSLRRVPTALGHRIVGLPTDDQGLDPASLPTGRDRKR
LDAVLITPSHQYPWGGSLSASRRAAVIEWAEMASCWIVEDDFDSELRHVGAPLPALASLDEEHTILLGTFSSVLTPALGC
GYLVVPPDLVHSFARQRRATGQPVPAIVQEAVADYLSTGALRRRTQRMRQVYRRRRRTVIDTLGSLPGTTLQPIDGGLHA
VLVFDGSEDDVVQRCRQEGVGVTPLSAYWGSSDAGVAGIVLGFGSHDDATLASALKIIAGVVTTHGRLSS
>Mature_470_residues
MRPDLVVDLPLHLPDDPRPLPVRICEEVRGLIMDGVLAPGDHLPSTRVLSAQLRVSRGTVVASYEQLEAEGYLVAAAGSG
TQINPDLRGLHRRSATTASVPRPQRHRGIDLTPGVPDTAGLANSAWRSAWHRSCVNPSRITDPLGSPHLRHEIAEHLRQM
RSLLADPRQVVVTGGARAGLAELIQVLCDGSRRLTIGVESPGYPSLRRVPTALGHRIVGLPTDDQGLDPASLPTGRDRKR
LDAVLITPSHQYPWGGSLSASRRAAVIEWAEMASCWIVEDDFDSELRHVGAPLPALASLDEEHTILLGTFSSVLTPALGC
GYLVVPPDLVHSFARQRRATGQPVPAIVQEAVADYLSTGALRRRTQRMRQVYRRRRRTVIDTLGSLPGTTLQPIDGGLHA
VLVFDGSEDDVVQRCRQEGVGVTPLSAYWGSSDAGVAGIVLGFGSHDDATLASALKIIAGVVTTHGRLSS

Specific function: May have a regulatory function in pyridoxine biosynthesis. Is said to also have an aminotransferase activity in valine biosynthesis as a double inactivation of ilvE and pdxR results in an auxotrophic requirement for valine [H]

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790797, Length=366, Percent_Identity=24.8633879781421, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000524
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 50538; Mature: 50538

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPDLVVDLPLHLPDDPRPLPVRICEEVRGLIMDGVLAPGDHLPSTRVLSAQLRVSRGTV
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHEEECCCCE
VASYEQLEAEGYLVAAAGSGTQINPDLRGLHRRSATTASVPRPQRHRGIDLTPGVPDTAG
EEHHHHHCCCCEEEEECCCCCEECHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCHHH
LANSAWRSAWHRSCVNPSRITDPLGSPHLRHEIAEHLRQMRSLLADPRQVVVTGGARAGL
HHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
AELIQVLCDGSRRLTIGVESPGYPSLRRVPTALGHRIVGLPTDDQGLDPASLPTGRDRKR
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHH
LDAVLITPSHQYPWGGSLSASRRAAVIEWAEMASCWIVEDDFDSELRHVGAPLPALASLD
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCHHHHHHHHCCCCHHHHCCC
EEHTILLGTFSSVLTPALGCGYLVVPPDLVHSFARQRRATGQPVPAIVQEAVADYLSTGA
CCCEEEEECHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LRRRTQRMRQVYRRRRRTVIDTLGSLPGTTLQPIDGGLHAVLVFDGSEDDVVQRCRQEGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCHHHHHHHHHCCC
GVTPLSAYWGSSDAGVAGIVLGFGSHDDATLASALKIIAGVVTTHGRLSS
CCCCHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRPDLVVDLPLHLPDDPRPLPVRICEEVRGLIMDGVLAPGDHLPSTRVLSAQLRVSRGTV
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHEEECCCCE
VASYEQLEAEGYLVAAAGSGTQINPDLRGLHRRSATTASVPRPQRHRGIDLTPGVPDTAG
EEHHHHHCCCCEEEEECCCCCEECHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCHHH
LANSAWRSAWHRSCVNPSRITDPLGSPHLRHEIAEHLRQMRSLLADPRQVVVTGGARAGL
HHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
AELIQVLCDGSRRLTIGVESPGYPSLRRVPTALGHRIVGLPTDDQGLDPASLPTGRDRKR
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHH
LDAVLITPSHQYPWGGSLSASRRAAVIEWAEMASCWIVEDDFDSELRHVGAPLPALASLD
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCHHHHHHHHCCCCHHHHCCC
EEHTILLGTFSSVLTPALGCGYLVVPPDLVHSFARQRRATGQPVPAIVQEAVADYLSTGA
CCCEEEEECHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LRRRTQRMRQVYRRRRRTVIDTLGSLPGTTLQPIDGGLHAVLVFDGSEDDVVQRCRQEGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCHHHHHHHHHCCC
GVTPLSAYWGSSDAGVAGIVLGFGSHDDATLASALKIIAGVVTTHGRLSS
CCCCHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948641; 12948626 [H]