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The map label for this gene is gutB [H]

Identifier: 50842402

GI number: 50842402

Start: 999077

End: 1000123

Strand: Reverse

Name: gutB [H]

Synonym: PPA0918

Alternate gene names: 50842402

Gene position: 1000123-999077 (Counterclockwise)

Preceding gene: 50842403

Following gene: 50842398

Centisome position: 39.06

GC content: 59.03

Gene sequence:

>1047_bases
ATGAAGGCATTGAGGTTCCACGCACCCGAGGACGTTCGTCTCGAAGACGTCCCCGAACCGGAATGCAAGTCCGACGAGAT
CAAGATCAAGGTTCGCAACTGCTCCACCTGTGGTACCGACGTCAAAATCCTTCACAACGGCCACCAGAACATCACCGGAG
TCACGATAATGGGCCACGAAGTTGCTGGCGAGATCGTCGAAATCGGCTCCGAGGTGGCTGGCGACTGGAAGATCGGCGAT
CGGGTCCAGTCGATCGCCGCTGTCCCGTGCGGGCAGTGCCACGAATGCCGCAAAGGGTGGATGCAGGTCTGCCAAAACCA
GACCAGCGTCGGTTATCAGTACGACGGTGGTTTTGCGGAATATATGATCGTCCCGCCGCAAGTACTCAAGGTAGACGGCC
TCAATCGCATCCCCGACGACGTCGATTTCGACGAGGCCAGCGCCGCCGAACCTTTCGCCTGCGCCATCAACGCCCAGGAA
CAACTCGGCATCGAAAAAGGTGACTTCCTCGCCATCTTTGGCGCCGGCCCGATCGGGTGCATGCACACCCGTATCGCGCG
TGGCGTGCACAAGGTCGGCACTATCGTCATGATTGACATCAACGCCGAACGCCTCGCATTGTCAGCCGCTGCCGTCAAGC
CCGACATCACCATCGACGGTTCACAGGAGGATGTCGTCGCCAAAGTCATGGAACTTACCAACGGTCGCGGTGCTGACGTC
ATCATCACCGCGACCCCGGCCAACATCACCCAGGAACAGGCCTTGTCCATGGCTGCCCGCAATGGCCGTATTTCTTTCTT
CGGCGGCCTGCCCAAGACGAACCCAACGATCACCTGCGACTCGAACCTGATCCATTACCGTCAACTCCACATCCACGGCG
CCAATGGCTCGGCCCCCGAGCACAACAAACGTGCCTTGCAGTACATCGCCTCGGGACAGGTTCCGGTTAAGGATCTCATC
ACCCGTCACGTCAAACTCGACGACGTCATGACGGTCTTCGATATCGTCGCCAAGGGCGAAGCCATCAAAGTGACCGTCGA
GCCCTGA

Upstream 100 bases:

>100_bases
ACCGCCCGGCAACTTCGTGAGGCGACATGCACCGACGAGGTCCTCAACCTCCTCGGTTCCCAGCAGGCATAATCAGCGGA
TCTATAGAAAGGCACACATC

Downstream 100 bases:

>100_bases
ACCCAACGAACCAAGTTCTCAATCGCAAGCGGTGAGGTTAGCCATAGCCCGCAACTGGGCTAAGCCGACCACACCGGCGG
TCGAGGTTCTCAATACCCCG

Product: putative zinc-binding sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKALRFHAPEDVRLEDVPEPECKSDEIKIKVRNCSTCGTDVKILHNGHQNITGVTIMGHEVAGEIVEIGSEVAGDWKIGD
RVQSIAAVPCGQCHECRKGWMQVCQNQTSVGYQYDGGFAEYMIVPPQVLKVDGLNRIPDDVDFDEASAAEPFACAINAQE
QLGIEKGDFLAIFGAGPIGCMHTRIARGVHKVGTIVMIDINAERLALSAAAVKPDITIDGSQEDVVAKVMELTNGRGADV
IITATPANITQEQALSMAARNGRISFFGGLPKTNPTITCDSNLIHYRQLHIHGANGSAPEHNKRALQYIASGQVPVKDLI
TRHVKLDDVMTVFDIVAKGEAIKVTVEP

Sequences:

>Translated_348_residues
MKALRFHAPEDVRLEDVPEPECKSDEIKIKVRNCSTCGTDVKILHNGHQNITGVTIMGHEVAGEIVEIGSEVAGDWKIGD
RVQSIAAVPCGQCHECRKGWMQVCQNQTSVGYQYDGGFAEYMIVPPQVLKVDGLNRIPDDVDFDEASAAEPFACAINAQE
QLGIEKGDFLAIFGAGPIGCMHTRIARGVHKVGTIVMIDINAERLALSAAAVKPDITIDGSQEDVVAKVMELTNGRGADV
IITATPANITQEQALSMAARNGRISFFGGLPKTNPTITCDSNLIHYRQLHIHGANGSAPEHNKRALQYIASGQVPVKDLI
TRHVKLDDVMTVFDIVAKGEAIKVTVEP
>Mature_348_residues
MKALRFHAPEDVRLEDVPEPECKSDEIKIKVRNCSTCGTDVKILHNGHQNITGVTIMGHEVAGEIVEIGSEVAGDWKIGD
RVQSIAAVPCGQCHECRKGWMQVCQNQTSVGYQYDGGFAEYMIVPPQVLKVDGLNRIPDDVDFDEASAAEPFACAINAQE
QLGIEKGDFLAIFGAGPIGCMHTRIARGVHKVGTIVMIDINAERLALSAAAVKPDITIDGSQEDVVAKVMELTNGRGADV
IITATPANITQEQALSMAARNGRISFFGGLPKTNPTITCDSNLIHYRQLHIHGANGSAPEHNKRALQYIASGQVPVKDLI
TRHVKLDDVMTVFDIVAKGEAIKVTVEP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=363, Percent_Identity=26.1707988980716, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI156523966, Length=313, Percent_Identity=25.5591054313099, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI4501939, Length=221, Percent_Identity=26.2443438914027, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790045, Length=353, Percent_Identity=31.4447592067989, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788075, Length=358, Percent_Identity=30.1675977653631, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1788073, Length=329, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI226510992, Length=346, Percent_Identity=25.4335260115607, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87082125, Length=361, Percent_Identity=25.7617728531856, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787863, Length=269, Percent_Identity=28.996282527881, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788407, Length=295, Percent_Identity=28.8135593220339, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI48994910, Length=362, Percent_Identity=26.5193370165746, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786825, Length=272, Percent_Identity=28.6764705882353, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081918, Length=324, Percent_Identity=28.7037037037037, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562876, Length=350, Percent_Identity=26.5714285714286, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17562878, Length=348, Percent_Identity=27.2988505747126, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71988145, Length=329, Percent_Identity=26.4437689969605, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=342, Percent_Identity=24.5614035087719, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17562584, Length=346, Percent_Identity=25.7225433526012, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=350, Percent_Identity=25.1428571428571, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319955, Length=350, Percent_Identity=25.1428571428571, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323099, Length=348, Percent_Identity=22.4137931034483, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=355, Percent_Identity=27.3239436619718, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=364, Percent_Identity=24.7252747252747, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323729, Length=348, Percent_Identity=25.5747126436782, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=356, Percent_Identity=26.4044943820225, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=353, Percent_Identity=24.3626062322946, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319257, Length=293, Percent_Identity=26.2798634812287, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17737897, Length=353, Percent_Identity=29.7450424929178, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137530, Length=349, Percent_Identity=28.6532951289398, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37582; Mature: 37582

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALRFHAPEDVRLEDVPEPECKSDEIKIKVRNCSTCGTDVKILHNGHQNITGVTIMGHE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCEEEEEECHH
VAGEIVEIGSEVAGDWKIGDRVQSIAAVPCGQCHECRKGWMQVCQNQTSVGYQYDGGFAE
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCEEE
YMIVPPQVLKVDGLNRIPDDVDFDEASAAEPFACAINAQEQLGIEKGDFLAIFGAGPIGC
EEEECCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCEEEEECCCCHHH
MHTRIARGVHKVGTIVMIDINAERLALSAAAVKPDITIDGSQEDVVAKVMELTNGRGADV
HHHHHHHHHHHCCEEEEEEECHHHEEEEHHHCCCCEEECCCHHHHHHHHHHHCCCCCCEE
IITATPANITQEQALSMAARNGRISFFGGLPKTNPTITCDSNLIHYRQLHIHGANGSAPE
EEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCEEEEEEEEEECCCCCCCC
HNKRALQYIASGQVPVKDLITRHVKLDDVMTVFDIVAKGEAIKVTVEP
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MKALRFHAPEDVRLEDVPEPECKSDEIKIKVRNCSTCGTDVKILHNGHQNITGVTIMGHE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCEEEEEECHH
VAGEIVEIGSEVAGDWKIGDRVQSIAAVPCGQCHECRKGWMQVCQNQTSVGYQYDGGFAE
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCEEE
YMIVPPQVLKVDGLNRIPDDVDFDEASAAEPFACAINAQEQLGIEKGDFLAIFGAGPIGC
EEEECCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCEEEEECCCCHHH
MHTRIARGVHKVGTIVMIDINAERLALSAAAVKPDITIDGSQEDVVAKVMELTNGRGADV
HHHHHHHHHHHCCEEEEEEECHHHEEEEHHHCCCCEEECCCHHHHHHHHHHHCCCCCCEE
IITATPANITQEQALSMAARNGRISFFGGLPKTNPTITCDSNLIHYRQLHIHGANGSAPE
EEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCEEEEEEEEEECCCCCCCC
HNKRALQYIASGQVPVKDLITRHVKLDDVMTVFDIVAKGEAIKVTVEP
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]