Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 50842373

GI number: 50842373

Start: 963450

End: 964262

Strand: Direct

Name: thiE [H]

Synonym: PPA0886

Alternate gene names: 50842373

Gene position: 963450-964262 (Clockwise)

Preceding gene: 50842372

Following gene: 50842374

Centisome position: 37.63

GC content: 63.35

Gene sequence:

>813_bases
ATGACCATCTCATCGCGGCCGTGTCCGCGACCGCCATGTTGTGTGTCGTCGCCGAACGGGCAGCTGAGCGCAGTGCTGGT
CCGGGGGCTTTCGCGCAAGCTCTCGTTGACGAATTATTCCTGGTGTCACCCGACGAGGTTGGTGAGCGGGGAGGGCTCCG
CGATGTCACGGCCTGAATTTGATCTGTCGGTTTATCTCGTCACCGACACCGCCCAGTGCGGCGGACCTGACGAGGTTGTT
GAGACCGTGCGCCACGCTATCGCCGGCGGGGTGACTCTGGTCCAATTCCGCGACCACGACTTGTCCGATGACGAGTTCGT
CGCTTTGGGGCGACGCGTCAGAGAGATCTGTGTGTCGGGAGGGGTGCCCCTCATCATTGATGACCGCGTACATCTCGTTG
CCGAAATTGGGGCCGATGGTGTCCATGTTGGGCAGTCTGACATGCCGGTCGACCAGGCCCGTGCGATTCTGGGCGACGAT
CTACTCATCGGCTTGTCCGCTCAGACTCCCGCCCATGTGGAGGCCGCCCTGTCCCAGGGGCGTGACATCGTCGACTATTT
GGGAGTTGGCGCCCTGCACGGCACTGGAACCAAACCTGAGGCTGGGGAGCTCGGCCTGGCTGGGATGCGCGATGTCGTCA
ACGCCAGCCCATGGCCGGTGTGCGTCATCGGTGGGGTGAGCGCATCCGATGCTCAAGACGTAGCCCGAGTGGGATGTGAC
GGCCTGAGCGTCGTCTCGGCGATTTGCCGGAGTACCGCCCCCAAGTCCAGTGCACGGGAACTTGCGGAGGCGTGGCGTAC
GGCGAAGGAATGA

Upstream 100 bases:

>100_bases
CGGCATGTGACCGTGTCATCGGGGCATGAGTGGATGACGCGGGTTACTGGAGTTGGATGCTCACTGGGAGCTCTCACGGC
AGCCTTTGCGGGAATCCAGA

Downstream 100 bases:

>100_bases
TGGCCATGACCTGGCGTGGAGACGTGCACAAATGGCCATAGATGCTGGCATCTTGTTCAGATGAGCGGACCTGGTGCGCA
GCGTTCTGAATCAGGCGCGA

Product: thiamin-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTISSRPCPRPPCCVSSPNGQLSAVLVRGLSRKLSLTNYSWCHPTRLVSGEGSAMSRPEFDLSVYLVTDTAQCGGPDEVV
ETVRHAIAGGVTLVQFRDHDLSDDEFVALGRRVREICVSGGVPLIIDDRVHLVAEIGADGVHVGQSDMPVDQARAILGDD
LLIGLSAQTPAHVEAALSQGRDIVDYLGVGALHGTGTKPEAGELGLAGMRDVVNASPWPVCVIGGVSASDAQDVARVGCD
GLSVVSAICRSTAPKSSARELAEAWRTAKE

Sequences:

>Translated_270_residues
MTISSRPCPRPPCCVSSPNGQLSAVLVRGLSRKLSLTNYSWCHPTRLVSGEGSAMSRPEFDLSVYLVTDTAQCGGPDEVV
ETVRHAIAGGVTLVQFRDHDLSDDEFVALGRRVREICVSGGVPLIIDDRVHLVAEIGADGVHVGQSDMPVDQARAILGDD
LLIGLSAQTPAHVEAALSQGRDIVDYLGVGALHGTGTKPEAGELGLAGMRDVVNASPWPVCVIGGVSASDAQDVARVGCD
GLSVVSAICRSTAPKSSARELAEAWRTAKE
>Mature_269_residues
TISSRPCPRPPCCVSSPNGQLSAVLVRGLSRKLSLTNYSWCHPTRLVSGEGSAMSRPEFDLSVYLVTDTAQCGGPDEVVE
TVRHAIAGGVTLVQFRDHDLSDDEFVALGRRVREICVSGGVPLIIDDRVHLVAEIGADGVHVGQSDMPVDQARAILGDDL
LIGLSAQTPAHVEAALSQGRDIVDYLGVGALHGTGTKPEAGELGLAGMRDVVNASPWPVCVIGGVSASDAQDVARVGCDG
LSVVSAICRSTAPKSSARELAEAWRTAKE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=212, Percent_Identity=36.7924528301887, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 28245; Mature: 28114

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISSRPCPRPPCCVSSPNGQLSAVLVRGLSRKLSLTNYSWCHPTRLVSGEGSAMSRPEF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC
DLSVYLVTDTAQCGGPDEVVETVRHAIAGGVTLVQFRDHDLSDDEFVALGRRVREICVSG
CEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
GVPLIIDDRVHLVAEIGADGVHVGQSDMPVDQARAILGDDLLIGLSAQTPAHVEAALSQG
CCCEEECCCEEEEEHHCCCCEEECCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCC
RDIVDYLGVGALHGTGTKPEAGELGLAGMRDVVNASPWPVCVIGGVSASDAQDVARVGCD
CHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHCCC
GLSVVSAICRSTAPKSSARELAEAWRTAKE
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TISSRPCPRPPCCVSSPNGQLSAVLVRGLSRKLSLTNYSWCHPTRLVSGEGSAMSRPEF
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC
DLSVYLVTDTAQCGGPDEVVETVRHAIAGGVTLVQFRDHDLSDDEFVALGRRVREICVSG
CEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
GVPLIIDDRVHLVAEIGADGVHVGQSDMPVDQARAILGDDLLIGLSAQTPAHVEAALSQG
CCCEEECCCEEEEEHHCCCCEEECCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCC
RDIVDYLGVGALHGTGTKPEAGELGLAGMRDVVNASPWPVCVIGGVSASDAQDVARVGCD
CHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHCCC
GLSVVSAICRSTAPKSSARELAEAWRTAKE
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA