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The map label for this gene is thiM [H]

Identifier: 50842372

GI number: 50842372

Start: 962597

End: 963625

Strand: Direct

Name: thiM [H]

Synonym: PPA0885

Alternate gene names: 50842372

Gene position: 962597-963625 (Clockwise)

Preceding gene: 50842371

Following gene: 50842373

Centisome position: 37.6

GC content: 62.88

Gene sequence:

>1029_bases
ATGCCGGCATGGTGTCACCCGGATCTTTGCCTGGTTGGAGCTGGTTTGGGCCGGCATTTCCCGATTAGTAGGGAGAGGAC
CGTCGTGAGCGCACGGCACGGCGGTAGGTTAGAAGACAACGACGCGGGAGCCCACTGGTCGCTGGGGGCTGAGAGGGCGC
CACGAAGGTGCCGACCGCCGGAACCTGTCCGGGTCATGCCGGCGAAGGGAGTGGTTGACATGACTGAATTGTCGAATTTG
GTGGCGGAATCTCTAGAGCGACTACGCCGAGACAAGCCGCTGGTGCAGTGCCTGACGAACATCGTCGTGGCCAATTTCAC
CGCTAACGTGTTGCTGTCTGCAGGGGCGGTTCCTGCCATGGTTGACAATCCCGAGGAATCCGAGGGATTCGCCTCCATTG
CCGACGGTGTACTCGTCAACACCGGAACTCCCTACCGGGAGACCACCGAGGCCATGATTGCTGCTGCGCGCGGTGCCGCA
AGCAGCAACACGCCATGGGTACTTGACCCGGTGGGGGTGGGCATGTCATGGCGCACTCGGGTGGTCCTGGATACCTTGGA
CGTCGCCGCTCCAGCCGTCATCCGGGCGAACGCCTCTGAGGTGTTGGCATTGGCCGGTACTGGTGCCTCCTCTCGAGGCC
CGGAGGCGGGAGATGCGGTTGAAGATGCCTTGGCGTCCGCGACTCAGCTCGTCCGCCATTACGGCTGCGTAGTGGCCATT
AGCGGCCCCGTCGATCACATCCTCGATGCGGATCGGCATGTGACCGTGTCATCGGGGCATGAGTGGATGACGCGGGTTAC
TGGAGTTGGATGCTCACTGGGAGCTCTCACGGCAGCCTTTGCGGGAATCCAGAATGACCATCTCATCGCGGCCGTGTCCG
CGACCGCCATGTTGTGTGTCGTCGCCGAACGGGCAGCTGAGCGCAGTGCTGGTCCGGGGGCTTTCGCGCAAGCTCTCGTT
GACGAATTATTCCTGGTGTCACCCGACGAGGTTGGTGAGCGGGGAGGGCTCCGCGATGTCACGGCCTGA

Upstream 100 bases:

>100_bases
TCTTTGTCCCCGACAGCTCTCCATGACAACGAGGTTTCGGTCTCGGCAACCTCTGACGTAGCAGCGTCGAGAACGTCAAC
GACAGGGTCAAGGCGGGGCT

Downstream 100 bases:

>100_bases
ATTTGATCTGTCGGTTTATCTCGTCACCGACACCGCCCAGTGCGGCGGACCTGACGAGGTTGTTGAGACCGTGCGCCACG
CTATCGCCGGCGGGGTGACT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MPAWCHPDLCLVGAGLGRHFPISRERTVVSARHGGRLEDNDAGAHWSLGAERAPRRCRPPEPVRVMPAKGVVDMTELSNL
VAESLERLRRDKPLVQCLTNIVVANFTANVLLSAGAVPAMVDNPEESEGFASIADGVLVNTGTPYRETTEAMIAAARGAA
SSNTPWVLDPVGVGMSWRTRVVLDTLDVAAPAVIRANASEVLALAGTGASSRGPEAGDAVEDALASATQLVRHYGCVVAI
SGPVDHILDADRHVTVSSGHEWMTRVTGVGCSLGALTAAFAGIQNDHLIAAVSATAMLCVVAERAAERSAGPGAFAQALV
DELFLVSPDEVGERGGLRDVTA

Sequences:

>Translated_342_residues
MPAWCHPDLCLVGAGLGRHFPISRERTVVSARHGGRLEDNDAGAHWSLGAERAPRRCRPPEPVRVMPAKGVVDMTELSNL
VAESLERLRRDKPLVQCLTNIVVANFTANVLLSAGAVPAMVDNPEESEGFASIADGVLVNTGTPYRETTEAMIAAARGAA
SSNTPWVLDPVGVGMSWRTRVVLDTLDVAAPAVIRANASEVLALAGTGASSRGPEAGDAVEDALASATQLVRHYGCVVAI
SGPVDHILDADRHVTVSSGHEWMTRVTGVGCSLGALTAAFAGIQNDHLIAAVSATAMLCVVAERAAERSAGPGAFAQALV
DELFLVSPDEVGERGGLRDVTA
>Mature_341_residues
PAWCHPDLCLVGAGLGRHFPISRERTVVSARHGGRLEDNDAGAHWSLGAERAPRRCRPPEPVRVMPAKGVVDMTELSNLV
AESLERLRRDKPLVQCLTNIVVANFTANVLLSAGAVPAMVDNPEESEGFASIADGVLVNTGTPYRETTEAMIAAARGAAS
SNTPWVLDPVGVGMSWRTRVVLDTLDVAAPAVIRANASEVLALAGTGASSRGPEAGDAVEDALASATQLVRHYGCVVAIS
GPVDHILDADRHVTVSSGHEWMTRVTGVGCSLGALTAAFAGIQNDHLIAAVSATAMLCVVAERAAERSAGPGAFAQALVD
ELFLVSPDEVGERGGLRDVTA

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=249, Percent_Identity=40.9638554216867, Blast_Score=172, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325042, Length=280, Percent_Identity=30.7142857142857, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 35630; Mature: 35499

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAWCHPDLCLVGAGLGRHFPISRERTVVSARHGGRLEDNDAGAHWSLGAERAPRRCRPP
CCCCCCCCHHEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEECCCHHCCCCCCCCC
EPVRVMPAKGVVDMTELSNLVAESLERLRRDKPLVQCLTNIVVANFTANVLLSAGAVPAM
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VDNPEESEGFASIADGVLVNTGTPYRETTEAMIAAARGAASSNTPWVLDPVGVGMSWRTR
CCCCCCCCCHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCE
VVLDTLDVAAPAVIRANASEVLALAGTGASSRGPEAGDAVEDALASATQLVRHYGCVVAI
EEEHHHHHHCCHHEECCHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEE
SGPVDHILDADRHVTVSSGHEWMTRVTGVGCSLGALTAAFAGIQNDHLIAAVSATAMLCV
CCCHHHHHCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
VAERAAERSAGPGAFAQALVDELFLVSPDEVGERGGLRDVTA
HHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
PAWCHPDLCLVGAGLGRHFPISRERTVVSARHGGRLEDNDAGAHWSLGAERAPRRCRPP
CCCCCCCHHEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEECCCHHCCCCCCCCC
EPVRVMPAKGVVDMTELSNLVAESLERLRRDKPLVQCLTNIVVANFTANVLLSAGAVPAM
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VDNPEESEGFASIADGVLVNTGTPYRETTEAMIAAARGAASSNTPWVLDPVGVGMSWRTR
CCCCCCCCCHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCE
VVLDTLDVAAPAVIRANASEVLALAGTGASSRGPEAGDAVEDALASATQLVRHYGCVVAI
EEEHHHHHHCCHHEECCHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEE
SGPVDHILDADRHVTVSSGHEWMTRVTGVGCSLGALTAAFAGIQNDHLIAAVSATAMLCV
CCCHHHHHCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
VAERAAERSAGPGAFAQALVDELFLVSPDEVGERGGLRDVTA
HHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA