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The map label for this gene is murE [H]

Identifier: 50842240

GI number: 50842240

Start: 827149

End: 828648

Strand: Direct

Name: murE [H]

Synonym: PPA0753

Alternate gene names: 50842240

Gene position: 827149-828648 (Clockwise)

Preceding gene: 50842239

Following gene: 50842241

Centisome position: 32.31

GC content: 61.4

Gene sequence:

>1500_bases
GTGGCCTCCTTCGACAGCTGCCGAGCTAATGACAACGGTCCTGCGATCACCGGGATCACATTGGACTCCCGCCAGGTTCG
CCCGGGCGATCTCTACGTCGGTCTGCACGGCCTGCATGCTCACGGGGCGAGTTTCGCTGCCTCTGCTGTTGAGTCGGGTG
CAGTGGCAGTGCTCACCGACGAGGACGGTGCCACACTGGCGGCGGAGGCGGGTGTGCCGATCATCGTCGTCGACGATCCG
CGCGCTGCCATGGCTGTTGCGGCCGCTAAGATCTTTGGACGCCCCTCGGATGCCATGACGATGTTTGGTATTACGGGGAC
TAACGGTAAAACCACAACGGCCTTCCTCGTGGAGGCCGGATTGCGTGCGGCTGGCCACCATGTTGGGACTATCGGGACAA
TTGGATTCCGGCTCGACGGTGAGGAGTTGCCTAGTGCTCGCACCACCGTCACGACGCCGGAGTCCCCAGATCTGGAAGGG
CTATTCGCGGCGATGGCTGAGCGAGGTGCTTCTGACGTCGTGATGGAGGTGTCGTCGCACGCTCTGGCCTTGCAGCGGGT
GGATGGAACCTGTTTCGATGTCGTGGCTTTCACCAACCTGGGCCGTGACCATCTGGATTTCCATAACACCCTTGATAATT
ATTTCAACGCTAAAGCCCGTTTGTTCACCCCCGATCTGGCTCGGGTTGGCGTTATCAATGTCGATGACCCGCGTGGTCGT
CAGCTAGTTGAGCGTATGCAAGCCAACGGGGTTAAGGCCGTGACAACGTCAATCCATCAGCCCTCTGACTATCAGGTGAC
GTCATGGCAGGCCGCGCGTGCCGGGGCAATTTTCGACGTGAGCACTCCGTCGGGGGAGCGCACCCTCGAGCTGTCGTTGC
CCGGCGAATTCAATGTCCGCAACGCAGCTATGGCTCTGGCCATGCTCGAGGCTGCCGGGCTCGCTTTTGACGATGTTGCA
GATGGTTTGTGTTCCGCGCAGGTGCCTGGCCGTATGGAGCGGGTCGATCTCGCAGATGACGCCCCGACGGCCCTCGTGGA
CTTTGCGCACACTCCGCAAGCGATTGCATCAGCTCTTGATGCCGCTCGGTCGTCAGTCGATGGCGGCCGTCTTATCGCCG
TGCTCGGTGCTGGGGGAGACCGTGACGTGGCCAAGCGTGGTCCAATGGGCCAGATTGCCGCTGAGCGTGCCGATATCGTC
ATCGTTACTGATGACAACCCACGTACCGAAGATCCTGCGGTGATCCGTGCAGCCGTGCTGGAGGGGGCCCATGAAGCTGC
TGGTGACGGGGTCATTGTGATCGACGGGGGACGTCGTCGTGAGGCGATTAACGAGGCCCTGCGGATGGCGGAGGCTGGCG
ACGTTATCTGCGTGCTCGGCAAGGGACATGAGACCGGGCAGAACATTGGCAGTGAGGTGCTGCCGTTCGACGATCGTGAG
GTTATCCGGGCCGAATGGAAGATCATGGCCCGGCAGAGCGAGGAGGATCAGGCACGATGA

Upstream 100 bases:

>100_bases
AGTACCAGCCGTGAGCCCTGAATCTACTGTCACCCCCACTCCACCTGCTGGTGCTGCCCTCCGACCATCCCAGGGTGATC
CTGTCGGGTGGACCGATCTA

Downstream 100 bases:

>100_bases
GAGTTCTCGACGTTGCCACCCTCGCGCAGTGGGTCGAAGCAGCACCGGTAAATGATTCTGCCCAGGTGGGCCCGGACGTC
GTCATTGATACCCGGAAGGT

Product: UDP-N-acetylmuramyl tripeptide synthase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MASFDSCRANDNGPAITGITLDSRQVRPGDLYVGLHGLHAHGASFAASAVESGAVAVLTDEDGATLAAEAGVPIIVVDDP
RAAMAVAAAKIFGRPSDAMTMFGITGTNGKTTTAFLVEAGLRAAGHHVGTIGTIGFRLDGEELPSARTTVTTPESPDLEG
LFAAMAERGASDVVMEVSSHALALQRVDGTCFDVVAFTNLGRDHLDFHNTLDNYFNAKARLFTPDLARVGVINVDDPRGR
QLVERMQANGVKAVTTSIHQPSDYQVTSWQAARAGAIFDVSTPSGERTLELSLPGEFNVRNAAMALAMLEAAGLAFDDVA
DGLCSAQVPGRMERVDLADDAPTALVDFAHTPQAIASALDAARSSVDGGRLIAVLGAGGDRDVAKRGPMGQIAAERADIV
IVTDDNPRTEDPAVIRAAVLEGAHEAAGDGVIVIDGGRRREAINEALRMAEAGDVICVLGKGHETGQNIGSEVLPFDDRE
VIRAEWKIMARQSEEDQAR

Sequences:

>Translated_499_residues
MASFDSCRANDNGPAITGITLDSRQVRPGDLYVGLHGLHAHGASFAASAVESGAVAVLTDEDGATLAAEAGVPIIVVDDP
RAAMAVAAAKIFGRPSDAMTMFGITGTNGKTTTAFLVEAGLRAAGHHVGTIGTIGFRLDGEELPSARTTVTTPESPDLEG
LFAAMAERGASDVVMEVSSHALALQRVDGTCFDVVAFTNLGRDHLDFHNTLDNYFNAKARLFTPDLARVGVINVDDPRGR
QLVERMQANGVKAVTTSIHQPSDYQVTSWQAARAGAIFDVSTPSGERTLELSLPGEFNVRNAAMALAMLEAAGLAFDDVA
DGLCSAQVPGRMERVDLADDAPTALVDFAHTPQAIASALDAARSSVDGGRLIAVLGAGGDRDVAKRGPMGQIAAERADIV
IVTDDNPRTEDPAVIRAAVLEGAHEAAGDGVIVIDGGRRREAINEALRMAEAGDVICVLGKGHETGQNIGSEVLPFDDRE
VIRAEWKIMARQSEEDQAR
>Mature_498_residues
ASFDSCRANDNGPAITGITLDSRQVRPGDLYVGLHGLHAHGASFAASAVESGAVAVLTDEDGATLAAEAGVPIIVVDDPR
AAMAVAAAKIFGRPSDAMTMFGITGTNGKTTTAFLVEAGLRAAGHHVGTIGTIGFRLDGEELPSARTTVTTPESPDLEGL
FAAMAERGASDVVMEVSSHALALQRVDGTCFDVVAFTNLGRDHLDFHNTLDNYFNAKARLFTPDLARVGVINVDDPRGRQ
LVERMQANGVKAVTTSIHQPSDYQVTSWQAARAGAIFDVSTPSGERTLELSLPGEFNVRNAAMALAMLEAAGLAFDDVAD
GLCSAQVPGRMERVDLADDAPTALVDFAHTPQAIASALDAARSSVDGGRLIAVLGAGGDRDVAKRGPMGQIAAERADIVI
VTDDNPRTEDPAVIRAAVLEGAHEAAGDGVIVIDGGRRREAINEALRMAEAGDVICVLGKGHETGQNIGSEVLPFDDREV
IRAEWKIMARQSEEDQAR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=475, Percent_Identity=37.6842105263158, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52250; Mature: 52119

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFDSCRANDNGPAITGITLDSRQVRPGDLYVGLHGLHAHGASFAASAVESGAVAVLTD
CCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEECCCCHHHHHHHCCCCEEEEEC
EDGATLAAEAGVPIIVVDDPRAAMAVAAAKIFGRPSDAMTMFGITGTNGKTTTAFLVEAG
CCCCEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEHHH
LRAAGHHVGTIGTIGFRLDGEELPSARTTVTTPESPDLEGLFAAMAERGASDVVMEVSSH
HHHCCCCCEEEEEEEEEECHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHH
ALALQRVDGTCFDVVAFTNLGRDHLDFHNTLDNYFNAKARLFTPDLARVGVINVDDPRGR
EEEEECCCCCEEEHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCHHHEEEEECCCCCHH
QLVERMQANGVKAVTTSIHQPSDYQVTSWQAARAGAIFDVSTPSGERTLELSLPGEFNVR
HHHHHHHHCCEEEEEEECCCCCCCEEECCHHHCCCEEEEECCCCCCEEEEEECCCCCCHH
NAAMALAMLEAAGLAFDDVADGLCSAQVPGRMERVDLADDAPTALVDFAHTPQAIASALD
HHHHHHHHHHHCCCCHHHHHCCHHCCCCCCCCEEEECCCCCCHHEEECCCCHHHHHHHHH
AARSSVDGGRLIAVLGAGGDRDVAKRGPMGQIAAERADIVIVTDDNPRTEDPAVIRAAVL
HHHHCCCCCEEEEEEECCCCCHHHHCCCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
EGAHEAAGDGVIVIDGGRRREAINEALRMAEAGDVICVLGKGHETGQNIGSEVLPFDDRE
HCCHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCCCCCCHH
VIRAEWKIMARQSEEDQAR
HHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
ASFDSCRANDNGPAITGITLDSRQVRPGDLYVGLHGLHAHGASFAASAVESGAVAVLTD
CCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEECCCCHHHHHHHCCCCEEEEEC
EDGATLAAEAGVPIIVVDDPRAAMAVAAAKIFGRPSDAMTMFGITGTNGKTTTAFLVEAG
CCCCEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEHHH
LRAAGHHVGTIGTIGFRLDGEELPSARTTVTTPESPDLEGLFAAMAERGASDVVMEVSSH
HHHCCCCCEEEEEEEEEECHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHH
ALALQRVDGTCFDVVAFTNLGRDHLDFHNTLDNYFNAKARLFTPDLARVGVINVDDPRGR
EEEEECCCCCEEEHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCHHHEEEEECCCCCHH
QLVERMQANGVKAVTTSIHQPSDYQVTSWQAARAGAIFDVSTPSGERTLELSLPGEFNVR
HHHHHHHHCCEEEEEEECCCCCCCEEECCHHHCCCEEEEECCCCCCEEEEEECCCCCCHH
NAAMALAMLEAAGLAFDDVADGLCSAQVPGRMERVDLADDAPTALVDFAHTPQAIASALD
HHHHHHHHHHHCCCCHHHHHCCHHCCCCCCCCEEEECCCCCCHHEEECCCCHHHHHHHHH
AARSSVDGGRLIAVLGAGGDRDVAKRGPMGQIAAERADIVIVTDDNPRTEDPAVIRAAVL
HHHHCCCCCEEEEEEECCCCCHHHHCCCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
EGAHEAAGDGVIVIDGGRRREAINEALRMAEAGDVICVLGKGHETGQNIGSEVLPFDDRE
HCCHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCCCCCCHH
VIRAEWKIMARQSEEDQAR
HHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]