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The map label for this gene is sucB [H]

Identifier: 50842181

GI number: 50842181

Start: 765084

End: 766457

Strand: Reverse

Name: sucB [H]

Synonym: PPA0693

Alternate gene names: 50842181

Gene position: 766457-765084 (Counterclockwise)

Preceding gene: 50842182

Following gene: 50842180

Centisome position: 29.94

GC content: 63.76

Gene sequence:

>1374_bases
ATGTCGACCGAAGTCACTCTTCCTGCACTGGGCGAGTCCGTCACCGAAGGCACCGTGTCCCGCTGGCTCAAGGCTGTCGG
AGACACCGTCGAGGCCGACGAACCCTTACTTGAGGTGTCCACCGACAAGGTCGATACCGAAGTCCCCTCCCCCGCCTCCG
GCACCCTACTTGAGATCAAGGTCCCCGAAGACGAAGACGCCGAAGTCGGCGCCGTCCTAGCCATCATCGGCGACCCCTCC
GAGTCGGGCTCTGCGCCTGCTAAGCCGACGGCTGAGCCCGCGGAAAAGGCCAAACCTGAGCCGGTGAAGAGCGAGGCCGA
GGAGGCACCGGCGCCGGCCGCGCCGAAGCCCGCCGAGGCTCCCAAACCCGCCGGGACCAATGAGGTCGCCCCACGCGCCA
CCAATCCGTCGTCGGACGTCTACGTCACCCCGCTAGTCCGCAAGCTCGCCCGGGAGAACAACGTCGACCTGTCGACGATC
ACCGGAACTGGTGTCGGGGGTCGTATTCGCAAGCAAGACGTACTAGCTGCAGCCGGTAAGTCTGGAGAAGCTCCCTCAGC
TCCTCAGGCACCGGCTGCCGCCCCTGCCCCGGCTGCTCCGAAACCGGCTGGTTCAGCCCGTAAAGCCTCCCAGGAAGTCT
CTCCGGAAGCTGCCGCTCTGCGCGGCACCACCGAGAAGATGAGCCGTCTACGCAAGGTCATCGCCTCGCGGATGGTTGAG
TCCCTGCAGATCTCGGCTCAGTTGACGGCCACCGTCGAGGTGGACATGACCGCTATCTCCCGCATCCGCAACGCTGAGAA
GGCTGCTTTCAAGGCCCGCGAGGGAGTCGGTCTGTCCTACTTGCCTTTCATTACCAAGGCCGTCGTTGAAGCCCTCAAGG
CCAATCCGACCTTCAACGCAAATATCGACACCGAGGCGGGGACGATTACCTACGGTTCTTCTGAGAACATCGGCATCGCC
GTTGATACCCCGCGCGGGCTGCTGGTTCCGGTCATCAAGAACGCTGGTGATCTCAATATCGCTGGGCTGGCCCACCAGAT
TGGCGATCTCGCTGCCCGCACCCGCGACAACAAGGTGACCCCTGACGAGCTGTCCGGCGGCACCTTCACCATCACCAACT
ATGGCTCGGCTGGAGCCCTCTTCGACACCCCGATCGTCAACCAGCCCGAGGTCGCCATCCTCGGCACCGGTGCTCTGGTT
AAGCGCCCGGTCGTCGTCACCAACGAGTTCGGCGAGGATACCATCGCAGTCCGCGACATGATGTACCTCTCGCTGTCCTA
CGATCACCGGCTCATTGACGGCGCTGTGGCGGCTCGCTTCCTATCGGGCATCAAGGCTCGTCTGGAGGAGGGCGACTTCG
CCGGAGAGTTCTGA

Upstream 100 bases:

>100_bases
GACATTTTCTGAATCCAAAAATGGGACCAACTGCACATTTGGCCACAGTCAAGCTAGGCTTGCCACGAATCCGTCGCGGC
TTGCAAAGGAGCTCCAACAC

Downstream 100 bases:

>100_bases
CCTTTGGAGACTAAGAAGAACGCGAGCCCGGGAAACTGAATACCCAGGGCAAGCCCGAGGCGGTGGGATCGATCCCACCG
CCTCGCCATTTATGCCCGCC

Product: dihydrolipoamide acyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSTEVTLPALGESVTEGTVSRWLKAVGDTVEADEPLLEVSTDKVDTEVPSPASGTLLEIKVPEDEDAEVGAVLAIIGDPS
ESGSAPAKPTAEPAEKAKPEPVKSEAEEAPAPAAPKPAEAPKPAGTNEVAPRATNPSSDVYVTPLVRKLARENNVDLSTI
TGTGVGGRIRKQDVLAAAGKSGEAPSAPQAPAAAPAPAAPKPAGSARKASQEVSPEAAALRGTTEKMSRLRKVIASRMVE
SLQISAQLTATVEVDMTAISRIRNAEKAAFKAREGVGLSYLPFITKAVVEALKANPTFNANIDTEAGTITYGSSENIGIA
VDTPRGLLVPVIKNAGDLNIAGLAHQIGDLAARTRDNKVTPDELSGGTFTITNYGSAGALFDTPIVNQPEVAILGTGALV
KRPVVVTNEFGEDTIAVRDMMYLSLSYDHRLIDGAVAARFLSGIKARLEEGDFAGEF

Sequences:

>Translated_457_residues
MSTEVTLPALGESVTEGTVSRWLKAVGDTVEADEPLLEVSTDKVDTEVPSPASGTLLEIKVPEDEDAEVGAVLAIIGDPS
ESGSAPAKPTAEPAEKAKPEPVKSEAEEAPAPAAPKPAEAPKPAGTNEVAPRATNPSSDVYVTPLVRKLARENNVDLSTI
TGTGVGGRIRKQDVLAAAGKSGEAPSAPQAPAAAPAPAAPKPAGSARKASQEVSPEAAALRGTTEKMSRLRKVIASRMVE
SLQISAQLTATVEVDMTAISRIRNAEKAAFKAREGVGLSYLPFITKAVVEALKANPTFNANIDTEAGTITYGSSENIGIA
VDTPRGLLVPVIKNAGDLNIAGLAHQIGDLAARTRDNKVTPDELSGGTFTITNYGSAGALFDTPIVNQPEVAILGTGALV
KRPVVVTNEFGEDTIAVRDMMYLSLSYDHRLIDGAVAARFLSGIKARLEEGDFAGEF
>Mature_456_residues
STEVTLPALGESVTEGTVSRWLKAVGDTVEADEPLLEVSTDKVDTEVPSPASGTLLEIKVPEDEDAEVGAVLAIIGDPSE
SGSAPAKPTAEPAEKAKPEPVKSEAEEAPAPAAPKPAEAPKPAGTNEVAPRATNPSSDVYVTPLVRKLARENNVDLSTIT
GTGVGGRIRKQDVLAAAGKSGEAPSAPQAPAAAPAPAAPKPAGSARKASQEVSPEAAALRGTTEKMSRLRKVIASRMVES
LQISAQLTATVEVDMTAISRIRNAEKAAFKAREGVGLSYLPFITKAVVEALKANPTFNANIDTEAGTITYGSSENIGIAV
DTPRGLLVPVIKNAGDLNIAGLAHQIGDLAARTRDNKVTPDELSGGTFTITNYGSAGALFDTPIVNQPEVAILGTGALVK
RPVVVTNEFGEDTIAVRDMMYLSLSYDHRLIDGAVAARFLSGIKARLEEGDFAGEF

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=460, Percent_Identity=31.9565217391304, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=41.5929203539823, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI110671329, Length=455, Percent_Identity=30.3296703296703, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI203098816, Length=471, Percent_Identity=28.4501061571125, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI203098753, Length=471, Percent_Identity=28.4501061571125, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI260898739, Length=136, Percent_Identity=36.7647058823529, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786946, Length=449, Percent_Identity=37.8619153674833, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786305, Length=451, Percent_Identity=32.1507760532151, Blast_Score=178, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=451, Percent_Identity=29.490022172949, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=466, Percent_Identity=33.0472103004292, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=225, Percent_Identity=37.7777777777778, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=321, Percent_Identity=28.6604361370716, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=449, Percent_Identity=33.184855233853, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=27.2331154684096, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI18859875, Length=448, Percent_Identity=30.1339285714286, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=40.7079646017699, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=30.7017543859649, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=30.5309734513274, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 47340; Mature: 47208

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEVTLPALGESVTEGTVSRWLKAVGDTVEADEPLLEVSTDKVDTEVPSPASGTLLEIK
CCCCEECHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEE
VPEDEDAEVGAVLAIIGDPSESGSAPAKPTAEPAEKAKPEPVKSEAEEAPAPAAPKPAEA
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCC
PKPAGTNEVAPRATNPSSDVYVTPLVRKLARENNVDLSTITGTGVGGRIRKQDVLAAAGK
CCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCC
SGEAPSAPQAPAAAPAPAAPKPAGSARKASQEVSPEAAALRGTTEKMSRLRKVIASRMVE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
SLQISAQLTATVEVDMTAISRIRNAEKAAFKAREGVGLSYLPFITKAVVEALKANPTFNA
HHEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
NIDTEAGTITYGSSENIGIAVDTPRGLLVPVIKNAGDLNIAGLAHQIGDLAARTRDNKVT
CCCCCCCEEEECCCCCEEEEEECCCCEEEHEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
PDELSGGTFTITNYGSAGALFDTPIVNQPEVAILGTGALVKRPVVVTNEFGEDTIAVRDM
HHHCCCCEEEEECCCCCCCEECCCCCCCCCEEEEECCHHHHCCEEEECCCCCCHHHHHHE
MYLSLSYDHRLIDGAVAARFLSGIKARLEEGDFAGEF
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STEVTLPALGESVTEGTVSRWLKAVGDTVEADEPLLEVSTDKVDTEVPSPASGTLLEIK
CCCEECHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEE
VPEDEDAEVGAVLAIIGDPSESGSAPAKPTAEPAEKAKPEPVKSEAEEAPAPAAPKPAEA
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCC
PKPAGTNEVAPRATNPSSDVYVTPLVRKLARENNVDLSTITGTGVGGRIRKQDVLAAAGK
CCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCC
SGEAPSAPQAPAAAPAPAAPKPAGSARKASQEVSPEAAALRGTTEKMSRLRKVIASRMVE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
SLQISAQLTATVEVDMTAISRIRNAEKAAFKAREGVGLSYLPFITKAVVEALKANPTFNA
HHEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
NIDTEAGTITYGSSENIGIAVDTPRGLLVPVIKNAGDLNIAGLAHQIGDLAARTRDNKVT
CCCCCCCEEEECCCCCEEEEEECCCCEEEHEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
PDELSGGTFTITNYGSAGALFDTPIVNQPEVAILGTGALVKRPVVVTNEFGEDTIAVRDM
HHHCCCCEEEEECCCCCCCEECCCCCCCCCEEEEECCHHHHCCEEEECCCCCCHHHHHHE
MYLSLSYDHRLIDGAVAARFLSGIKARLEEGDFAGEF
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]