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The map label for this gene is lipA

Identifier: 50842177

GI number: 50842177

Start: 758856

End: 759848

Strand: Reverse

Name: lipA

Synonym: PPA0689

Alternate gene names: 50842177

Gene position: 759848-758856 (Counterclockwise)

Preceding gene: 50842178

Following gene: 50842176

Centisome position: 29.68

GC content: 59.92

Gene sequence:

>993_bases
GTGAGTGTCGAAGCAGACGGTAGAAAGCTCCTCCGAGTCGAGGTCAAGAATTCTCAGACGCCCATCGAGAACAAACCCCA
CTGGATCAAGACTCGGGCCACAATGGGTCCGGAGTACCGCGACATGCGTCGCCGAATGATCGACGGAGATCTGCACACCG
TGTGTCAAGAGGCTGGCTGCCCCAATATTTTCGAGTGTTGGGAGGATCGCGAGGCCACTTTCCTCATCGGCGGCGACCAC
TGCACCCGCCGTTGTGACTTCTGCCAGATCGAGTCGGCCAAGCCGGAAGGCTACGACAAAGACGAGCCGCGTCGCGTCGC
AGAGTCCGTCAAGCAGATGGGGCTGCGCTACGCTACCGTTACGTCGGTATGCCGAGACGACCTCGAGGACGAAGGTGCCT
GGCTGTGCGCCGAGACGATTCGTCAAATCCACCAGGTAACTCCCGGCGTCGGCGTCGAGATGTTAGCCCAAGATTTTTCC
GGCAAACAGGACCTGCTCGACATCGTCTTCGAAGCACGCCCGGAGGTCTTCGGTCACAACCTTGAGACGGTCCCCCGCAT
CTTCAAGCGCATTCGACCCGGATTCCGCTACGACCGTTCTTTGGCCGTCCTGGACTCTGCACATGAGGCCGGTCTGATCA
CCAAGTCAAATCTCATTCTTGGCATGGGCGAGACCCGTGAAGAAATTTCACAGGCCATGCGGGCGCTCCATGACGCCAAC
ACCGACCTGCTGACGATCACCCAGTACCTGCGCCCGAACTCCTACCTACACCCCATCGACCGTTGGGTGACGCCTCAGGA
GTTCGATGAGTTAGCCGAAGAGGCCCGCGAAATTGGATTTGTCGGTGTGATGAGCGGACCGCTTGTGCGATCCTCCTACC
GGGCTGGCCGCCTCTATCGGCAAGCTATGGAAGCACGCGGCGAGCGTCCGGTGATGATCGTGACCGGGTACCGCGACGGT
GCGAAACCTGCACCATTCGCGGCCAAGGAGTGA

Upstream 100 bases:

>100_bases
CCGCTGATATCCGGGCTTGCCATGAGCCTTTCGAGCCTCTTCGTCGTCACCCATTCCCTGAGACTTCGTAACTTCGGCAC
ACGCAGTTAAACTGGCCGGC

Downstream 100 bases:

>100_bases
CGGTGCGACCTCTCCTAGAGCTGGGACGCGCGTCGAACGGCTCACATGAGGTGAGGCCGCCCCTGCATCAAAGTCACAAC
ATCGAATAGCCATACAACGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSVEADGRKLLRVEVKNSQTPIENKPHWIKTRATMGPEYRDMRRRMIDGDLHTVCQEAGCPNIFECWEDREATFLIGGDH
CTRRCDFCQIESAKPEGYDKDEPRRVAESVKQMGLRYATVTSVCRDDLEDEGAWLCAETIRQIHQVTPGVGVEMLAQDFS
GKQDLLDIVFEARPEVFGHNLETVPRIFKRIRPGFRYDRSLAVLDSAHEAGLITKSNLILGMGETREEISQAMRALHDAN
TDLLTITQYLRPNSYLHPIDRWVTPQEFDELAEEAREIGFVGVMSGPLVRSSYRAGRLYRQAMEARGERPVMIVTGYRDG
AKPAPFAAKE

Sequences:

>Translated_330_residues
MSVEADGRKLLRVEVKNSQTPIENKPHWIKTRATMGPEYRDMRRRMIDGDLHTVCQEAGCPNIFECWEDREATFLIGGDH
CTRRCDFCQIESAKPEGYDKDEPRRVAESVKQMGLRYATVTSVCRDDLEDEGAWLCAETIRQIHQVTPGVGVEMLAQDFS
GKQDLLDIVFEARPEVFGHNLETVPRIFKRIRPGFRYDRSLAVLDSAHEAGLITKSNLILGMGETREEISQAMRALHDAN
TDLLTITQYLRPNSYLHPIDRWVTPQEFDELAEEAREIGFVGVMSGPLVRSSYRAGRLYRQAMEARGERPVMIVTGYRDG
AKPAPFAAKE
>Mature_329_residues
SVEADGRKLLRVEVKNSQTPIENKPHWIKTRATMGPEYRDMRRRMIDGDLHTVCQEAGCPNIFECWEDREATFLIGGDHC
TRRCDFCQIESAKPEGYDKDEPRRVAESVKQMGLRYATVTSVCRDDLEDEGAWLCAETIRQIHQVTPGVGVEMLAQDFSG
KQDLLDIVFEARPEVFGHNLETVPRIFKRIRPGFRYDRSLAVLDSAHEAGLITKSNLILGMGETREEISQAMRALHDANT
DLLTITQYLRPNSYLHPIDRWVTPQEFDELAEEAREIGFVGVMSGPLVRSSYRAGRLYRQAMEARGERPVMIVTGYRDGA
KPAPFAAKE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=37.9310344827586, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI37577164, Length=236, Percent_Identity=38.9830508474576, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1786846, Length=308, Percent_Identity=42.2077922077922, Blast_Score=243, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=37.7622377622378, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=40.6360424028269, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI221513272, Length=294, Percent_Identity=39.1156462585034, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_PROAC (Q6A9X0)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055404.1
- ProteinModelPortal:   Q6A9X0
- GeneID:   2932464
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0689
- NMPDR:   fig|267747.1.peg.681
- HOGENOM:   HBG284542
- OMA:   KTKIPMG
- ProtClustDB:   PRK05481
- BioCyc:   PACN267747:PPA0689-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 37521; Mature: 37390

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEADGRKLLRVEVKNSQTPIENKPHWIKTRATMGPEYRDMRRRMIDGDLHTVCQEAGC
CCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCC
PNIFECWEDREATFLIGGDHCTRRCDFCQIESAKPEGYDKDEPRRVAESVKQMGLRYATV
CCHHHHHCCCCEEEEEECCHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
TSVCRDDLEDEGAWLCAETIRQIHQVTPGVGVEMLAQDFSGKQDLLDIVFEARPEVFGHN
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCHHHHCCC
LETVPRIFKRIRPGFRYDRSLAVLDSAHEAGLITKSNLILGMGETREEISQAMRALHDAN
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEECCEEEECCCCHHHHHHHHHHHHHCC
TDLLTITQYLRPNSYLHPIDRWVTPQEFDELAEEAREIGFVGVMSGPLVRSSYRAGRLYR
CCHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHH
QAMEARGERPVMIVTGYRDGAKPAPFAAKE
HHHHHCCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVEADGRKLLRVEVKNSQTPIENKPHWIKTRATMGPEYRDMRRRMIDGDLHTVCQEAGC
CCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCC
PNIFECWEDREATFLIGGDHCTRRCDFCQIESAKPEGYDKDEPRRVAESVKQMGLRYATV
CCHHHHHCCCCEEEEEECCHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
TSVCRDDLEDEGAWLCAETIRQIHQVTPGVGVEMLAQDFSGKQDLLDIVFEARPEVFGHN
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCHHHHCCC
LETVPRIFKRIRPGFRYDRSLAVLDSAHEAGLITKSNLILGMGETREEISQAMRALHDAN
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEECCEEEECCCCHHHHHHHHHHHHHCC
TDLLTITQYLRPNSYLHPIDRWVTPQEFDELAEEAREIGFVGVMSGPLVRSSYRAGRLYR
CCHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHH
QAMEARGERPVMIVTGYRDGAKPAPFAAKE
HHHHHCCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA