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The map label for this gene is glnA [H]

Identifier: 50842158

GI number: 50842158

Start: 738690

End: 740111

Strand: Reverse

Name: glnA [H]

Synonym: PPA0671

Alternate gene names: 50842158

Gene position: 740111-738690 (Counterclockwise)

Preceding gene: 50842162

Following gene: 50842155

Centisome position: 28.91

GC content: 59.07

Gene sequence:

>1422_bases
ATGTTCGAAGGTCCCGACGACCTGCTTGCCTATGTCAAGAAGGAGGGCATCGAGATGATCGATGTCCGCTTCTGTGACCT
TATCGGTATCATGCAGCACTTCACCGTTCCCGCCCGAGAGTTCGACAAGGACCAGTACGAGAACGGTCTGGCCTTCGACG
GATCCTCTATTCGGGGTTTCCAGAAGATTAACGAGTCGGACATGGCCCTACTACCCGATCCGACGACCGCCTGGATCGAC
CCCTTCCGCGAGCGCAAAACCCTTATCGTGAATTTCAACGTCCATGACCCCATTACGAAGGAGGCCTACAGCCGCGATCC
GCGCAACATCGCGCGTAAGGCCGAGGCTTACCTCGCATCTACTGGGATCGGCGATACTGCCTTCTTCGCTCCTGAAGCTG
AGTTTTACGTCTTCGACGACATTCGCTTCGACACCCGTCAGAACACCTCGTACTACTCCATCGACTCCGAGGCTGGAGCG
TGGAACACTGGACGTACCGAGGATGGCGGCAACCGCGGATACAAGGTGAAATACAAGGGCGGATATTTTCCGGTCGCCCC
GACCGACCATTTTGGTGATCTGCGTGACGAAATCGTCACCATCATGGAGCGCCTTGGCATGAAGGTCGAGCGCGCCCACC
ATGAGGTGGGCACAGCAGGTCAAGCCGAGATCAATTGGCGTTTCGACACCCTGTCGCGCAGCGCTGACGATGTCATGAAG
TTTAAGTACATCGTCAAGAACGTCGCCTGGCAGCACGGCAAGACGGCCACTTTCATGCCGAAGCCGATCTTCGGAGACAA
CGGCTCGGGCATGCATGTTCACTCTTCGATCTGGAAGGGCGGCGAACCTCTGTTCTTTGACGAGTCCGGATACGCCCAAC
TCTCTGACATGGCCCGCTACTACATCGGCGGCATCCTCAAGCACGCACCGTCGCTGCTGGCCTTCACTAACCCCTCGGCC
AACTCGTACCACCGTCTGGTGCCGGGCTTCGAGGCCCCAGTCAATCTGGTGTATTCACAGCGCAACCGCTCCGCCTGCAT
GCGTATCCCGATCACCGGCCCAAATCCGAAGGCCAAGCGCGTCGAATTCCGTTGCCCAGATCCGTCTGCCAACCCTTACA
TGGCCTTTGCCGCCCTGCTACTGGCCGGCCTGGACGGCATCCAGAACAAGATCGAGCCGCCCGCGCCGGTCGACAAGGAC
ATCTACGAGCTCCCTCCTGAGGAGCATGCCGCCATGGATCACGTGCCGGGCAACCTGGGTGCGGTCCTCGACAATCTCGA
GGCCGACCACGAGTTTCTCCTTGCCGGTGACGTGTTCACCCCGGATCTCATCGAGACCTGGGTCGAACTCAAGCGTGGCG
ACCTGGCTGCCCTGCAGCAACGTCCGCACCCCTACGAGTTCGACCTGTACTACGACATCTGA

Upstream 100 bases:

>100_bases
CCGGCACGACCTGTTACACGCTCGAAACACAACAGTGACGGTCGAGCAACTCATACGAACTACCGTAAAGCACATCACCA
GTCATGTTGATGGAGGAAAG

Downstream 100 bases:

>100_bases
GTCGTTCTACTCGCCGGGGGCGCAACTACGGTTGCTCCCCTGGCGGCGTATAAAACAAGCCTCGAACTACACACCCCCAA
CTCAGTCCGACCATCTCACT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MFEGPDDLLAYVKKEGIEMIDVRFCDLIGIMQHFTVPAREFDKDQYENGLAFDGSSIRGFQKINESDMALLPDPTTAWID
PFRERKTLIVNFNVHDPITKEAYSRDPRNIARKAEAYLASTGIGDTAFFAPEAEFYVFDDIRFDTRQNTSYYSIDSEAGA
WNTGRTEDGGNRGYKVKYKGGYFPVAPTDHFGDLRDEIVTIMERLGMKVERAHHEVGTAGQAEINWRFDTLSRSADDVMK
FKYIVKNVAWQHGKTATFMPKPIFGDNGSGMHVHSSIWKGGEPLFFDESGYAQLSDMARYYIGGILKHAPSLLAFTNPSA
NSYHRLVPGFEAPVNLVYSQRNRSACMRIPITGPNPKAKRVEFRCPDPSANPYMAFAALLLAGLDGIQNKIEPPAPVDKD
IYELPPEEHAAMDHVPGNLGAVLDNLEADHEFLLAGDVFTPDLIETWVELKRGDLAALQQRPHPYEFDLYYDI

Sequences:

>Translated_473_residues
MFEGPDDLLAYVKKEGIEMIDVRFCDLIGIMQHFTVPAREFDKDQYENGLAFDGSSIRGFQKINESDMALLPDPTTAWID
PFRERKTLIVNFNVHDPITKEAYSRDPRNIARKAEAYLASTGIGDTAFFAPEAEFYVFDDIRFDTRQNTSYYSIDSEAGA
WNTGRTEDGGNRGYKVKYKGGYFPVAPTDHFGDLRDEIVTIMERLGMKVERAHHEVGTAGQAEINWRFDTLSRSADDVMK
FKYIVKNVAWQHGKTATFMPKPIFGDNGSGMHVHSSIWKGGEPLFFDESGYAQLSDMARYYIGGILKHAPSLLAFTNPSA
NSYHRLVPGFEAPVNLVYSQRNRSACMRIPITGPNPKAKRVEFRCPDPSANPYMAFAALLLAGLDGIQNKIEPPAPVDKD
IYELPPEEHAAMDHVPGNLGAVLDNLEADHEFLLAGDVFTPDLIETWVELKRGDLAALQQRPHPYEFDLYYDI
>Mature_473_residues
MFEGPDDLLAYVKKEGIEMIDVRFCDLIGIMQHFTVPAREFDKDQYENGLAFDGSSIRGFQKINESDMALLPDPTTAWID
PFRERKTLIVNFNVHDPITKEAYSRDPRNIARKAEAYLASTGIGDTAFFAPEAEFYVFDDIRFDTRQNTSYYSIDSEAGA
WNTGRTEDGGNRGYKVKYKGGYFPVAPTDHFGDLRDEIVTIMERLGMKVERAHHEVGTAGQAEINWRFDTLSRSADDVMK
FKYIVKNVAWQHGKTATFMPKPIFGDNGSGMHVHSSIWKGGEPLFFDESGYAQLSDMARYYIGGILKHAPSLLAFTNPSA
NSYHRLVPGFEAPVNLVYSQRNRSACMRIPITGPNPKAKRVEFRCPDPSANPYMAFAALLLAGLDGIQNKIEPPAPVDKD
IYELPPEEHAAMDHVPGNLGAVLDNLEADHEFLLAGDVFTPDLIETWVELKRGDLAALQQRPHPYEFDLYYDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=470, Percent_Identity=23.4042553191489, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=51.4957264957265, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI87081870, Length=291, Percent_Identity=28.8659793814433, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53261; Mature: 53261

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEGPDDLLAYVKKEGIEMIDVRFCDLIGIMQHFTVPAREFDKDQYENGLAFDGSSIRGF
CCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCEECCCCCCHH
QKINESDMALLPDPTTAWIDPFRERKTLIVNFNVHDPITKEAYSRDPRNIARKAEAYLAS
HHCCCCCCEECCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHH
TGIGDTAFFAPEAEFYVFDDIRFDTRQNTSYYSIDSEAGAWNTGRTEDGGNRGYKVKYKG
CCCCCCEEECCCCCEEEEECEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECC
GYFPVAPTDHFGDLRDEIVTIMERLGMKVERAHHEVGTAGQAEINWRFDTLSRSADDVMK
CEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHH
FKYIVKNVAWQHGKTATFMPKPIFGDNGSGMHVHSSIWKGGEPLFFDESGYAQLSDMARY
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEHHHCCCCCEEEECCCCCHHHHHHHHH
YIGGILKHAPSLLAFTNPSANSYHRLVPGFEAPVNLVYSQRNRSACMRIPITGPNPKAKR
HHHHHHHHCCCEEEEECCCCCCCHHCCCCCCCCHHHEEECCCCCCEEEEECCCCCCCCCE
VEFRCPDPSANPYMAFAALLLAGLDGIQNKIEPPAPVDKDIYELPPEEHAAMDHVPGNLG
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHCCCCHHH
AVLDNLEADHEFLLAGDVFTPDLIETWVELKRGDLAALQQRPHPYEFDLYYDI
HHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEEEC
>Mature Secondary Structure
MFEGPDDLLAYVKKEGIEMIDVRFCDLIGIMQHFTVPAREFDKDQYENGLAFDGSSIRGF
CCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCEECCCCCCHH
QKINESDMALLPDPTTAWIDPFRERKTLIVNFNVHDPITKEAYSRDPRNIARKAEAYLAS
HHCCCCCCEECCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHH
TGIGDTAFFAPEAEFYVFDDIRFDTRQNTSYYSIDSEAGAWNTGRTEDGGNRGYKVKYKG
CCCCCCEEECCCCCEEEEECEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECC
GYFPVAPTDHFGDLRDEIVTIMERLGMKVERAHHEVGTAGQAEINWRFDTLSRSADDVMK
CEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHH
FKYIVKNVAWQHGKTATFMPKPIFGDNGSGMHVHSSIWKGGEPLFFDESGYAQLSDMARY
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEHHHCCCCCEEEECCCCCHHHHHHHHH
YIGGILKHAPSLLAFTNPSANSYHRLVPGFEAPVNLVYSQRNRSACMRIPITGPNPKAKR
HHHHHHHHCCCEEEEECCCCCCCHHCCCCCCCCHHHEEECCCCCCEEEEECCCCCCCCCE
VEFRCPDPSANPYMAFAALLLAGLDGIQNKIEPPAPVDKDIYELPPEEHAAMDHVPGNLG
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHCCCCHHH
AVLDNLEADHEFLLAGDVFTPDLIETWVELKRGDLAALQQRPHPYEFDLYYDI
HHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8885396 [H]