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The map label for this gene is lacF [H]

Identifier: 50842092

GI number: 50842092

Start: 669182

End: 669667

Strand: Direct

Name: lacF [H]

Synonym: PPA0605

Alternate gene names: 50842092

Gene position: 669182-669667 (Clockwise)

Preceding gene: 50842091

Following gene: 50842093

Centisome position: 26.14

GC content: 53.29

Gene sequence:

>486_bases
ATGACAATGCTTCCCCTGATCCTCGGTGTTTTTGTCAAAGAGCATGTCCCCGGTATTGGCATTTTTCGTACTCTCTTCTA
CCTGTCGGCCATCTCATCTCTGGTGGTCATCGCATTGGCGTGGAGTGCGATCCTCAAGGACAACGGTCTGGTCAACAACT
TCCTCGTTGGCTCCGGCTTGATCAACTCCCCGGTGCCGTTCCTCACTGGGCGCTGGTGGTTGATCATCTCGTCGTGCCTC
ATAACCCTGTGGTCTGGAGTTCCCTACTACATGCTGATGTATCTGACAGCGCTGGCGAATATCGACAAGCTCCTCTACGA
GGCCGCAGTCATCGATGGTGCAGGCGCTGTGAAGTCCTTCTTTACGGTCACCGTCCCCGGCATGAAGATTATGATGGCCC
TTGTCTCAATCCTCTCGATGATCGGCTGCCGCCGTCTTCCTCTTCGTCATGACCGTTGGTTTCATCATTATCGGTCAGGT
TTTTAA

Upstream 100 bases:

>100_bases
CTGGGTCGGTTTTGGAAATTACGGAAAAATCTTCGCAGACGACGCATTATGGAATGCTGTTCTGAACTCAATCGTCTACG
CGATTATCGTCGTCCCGTTA

Downstream 100 bases:

>100_bases
CCGTCGCTCTGGTGAGAAGTGATACCGGTGACCACATCCACTGTTTTTCCTGCGAGTTCGGCACTGTGCGTGCGCAAACG
CTCCATCAACAAGTCCAAAT

Product: putative sugar transport protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTMLPLILGVFVKEHVPGIGIFRTLFYLSAISSLVVIALAWSAILKDNGLVNNFLVGSGLINSPVPFLTGRWWLIISSCL
ITLWSGVPYYMLMYLTALANIDKLLYEAAVIDGAGAVKSFFTVTVPGMKIMMALVSILSMIGCRRLPLRHDRWFHHYRSG
F

Sequences:

>Translated_161_residues
MTMLPLILGVFVKEHVPGIGIFRTLFYLSAISSLVVIALAWSAILKDNGLVNNFLVGSGLINSPVPFLTGRWWLIISSCL
ITLWSGVPYYMLMYLTALANIDKLLYEAAVIDGAGAVKSFFTVTVPGMKIMMALVSILSMIGCRRLPLRHDRWFHHYRSG
F
>Mature_160_residues
TMLPLILGVFVKEHVPGIGIFRTLFYLSAISSLVVIALAWSAILKDNGLVNNFLVGSGLINSPVPFLTGRWWLIISSCLI
TLWSGVPYYMLMYLTALANIDKLLYEAAVIDGAGAVKSFFTVTVPGMKIMMALVSILSMIGCRRLPLRHDRWFHHYRSGF

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=110, Percent_Identity=38.1818181818182, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 17925; Mature: 17794

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLPLILGVFVKEHVPGIGIFRTLFYLSAISSLVVIALAWSAILKDNGLVNNFLVGSGL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
INSPVPFLTGRWWLIISSCLITLWSGVPYYMLMYLTALANIDKLLYEAAVIDGAGAVKSF
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FTVTVPGMKIMMALVSILSMIGCRRLPLRHDRWFHHYRSGF
HHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TMLPLILGVFVKEHVPGIGIFRTLFYLSAISSLVVIALAWSAILKDNGLVNNFLVGSGL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
INSPVPFLTGRWWLIISSCLITLWSGVPYYMLMYLTALANIDKLLYEAAVIDGAGAVKSF
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FTVTVPGMKIMMALVSILSMIGCRRLPLRHDRWFHHYRSGF
HHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]