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The map label for this gene is ispH

Identifier: 50842058

GI number: 50842058

Start: 632907

End: 633878

Strand: Direct

Name: ispH

Synonym: PPA0572

Alternate gene names: 50842058

Gene position: 632907-633878 (Clockwise)

Preceding gene: 50842053

Following gene: 50842060

Centisome position: 24.72

GC content: 61.01

Gene sequence:

>972_bases
ATGGCTGTTACGGGGGCGTGCACGAATAAGAAGCTGTACTTAGCTGCTCCGCGTGGATACTGCGCAGGCGTTGATCGTGC
GGTGGTGACCGTTGAGAAGGCGCTAGAGGTGTATGGCGCTCCGGTGTACGTGCGCAAGCAGATCGTCCACAACCGCCACG
TCGTGGAGACCTTGGAGGGGCGCGGAGCGATCTTCGTTGATGAGCTCGACGAAGTGCCCGACGATGCCCTCGTGGTGTTC
TCGGCTCACGGCGTGTCCCCGCAAGTTAAGAAAGAGGCCACCGATCGTGGTCTTCGTACTATTGACGCAACGTGCCCACT
GGTGACGAAGGTGCATCACGAGGCCAAAAGGTTCGCTAACCAAGACACCCAGATCCTCCTTATTGGTCATGCCGGTCACG
AGGAGGTCGAGGGGACGACTGGCGAGGCTCCCGAGAACATCACCCTGGTGCAGACTCCCGCGGATGTTGATGGGTTGGCG
TTAGACCGCGACCGTCCCGTGGCGTGGCTGTCCCAGACAACGCTGAGTGTCGACGAGACGGTGGAGACCGTCGATCGGAT
TCGCGACCTACATCCTCAGCTCATCGATCCGCCTTCCGACGACATCTGCTACGCCACCCAGAACCGGCAGCACGCCGTCA
AGCAGATGGCTCCTCAATGTGACCTTGTCATCGTGGTGGGATCGAAGAACTCGTCCAACACTGGCCGTTTGGTGGAGGTC
GCCCTCGAATCGGGGGCCAAAGCCTCCTATCGGGTGGATAACGCAGGAGAGATCGAGGAGACCTGGCTCGACGGTGTGTC
CACCATCGGCGTGACCTCGGGGGCATCGGTGCCGGAGGCCCTCGTCCAGGGGGTGATCGATCTGCTCATCTCGAAGGGTT
GGCCGCCGGCTGAGGAAGAGACCCTCATTGAGGAGTCGTTGTCCTTCGCCTTGCCGCCTCAACTGCGAAAACGTCGCACG
ACGGCCAAGTGA

Upstream 100 bases:

>100_bases
CACCTTCAGGGAATTTTAGGTAAACGGACTTTCTGCGGGTCCTCGGCAGGCGAACTTGAACGGGTTCGTCAGATGCCGAC
GTACCCGTAGACTCAGGATC

Downstream 100 bases:

>100_bases
GGTTCATTCCGGAAGGCCCACCTCAGTCTGGTTCTCGGTCAGTTCCGGCGAGGTGAGGGGGCGTACCGCCTCTTCGATGG
GGGCCGGGGACGCGATGTCG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAVTGACTNKKLYLAAPRGYCAGVDRAVVTVEKALEVYGAPVYVRKQIVHNRHVVETLEGRGAIFVDELDEVPDDALVVF
SAHGVSPQVKKEATDRGLRTIDATCPLVTKVHHEAKRFANQDTQILLIGHAGHEEVEGTTGEAPENITLVQTPADVDGLA
LDRDRPVAWLSQTTLSVDETVETVDRIRDLHPQLIDPPSDDICYATQNRQHAVKQMAPQCDLVIVVGSKNSSNTGRLVEV
ALESGAKASYRVDNAGEIEETWLDGVSTIGVTSGASVPEALVQGVIDLLISKGWPPAEEETLIEESLSFALPPQLRKRRT
TAK

Sequences:

>Translated_323_residues
MAVTGACTNKKLYLAAPRGYCAGVDRAVVTVEKALEVYGAPVYVRKQIVHNRHVVETLEGRGAIFVDELDEVPDDALVVF
SAHGVSPQVKKEATDRGLRTIDATCPLVTKVHHEAKRFANQDTQILLIGHAGHEEVEGTTGEAPENITLVQTPADVDGLA
LDRDRPVAWLSQTTLSVDETVETVDRIRDLHPQLIDPPSDDICYATQNRQHAVKQMAPQCDLVIVVGSKNSSNTGRLVEV
ALESGAKASYRVDNAGEIEETWLDGVSTIGVTSGASVPEALVQGVIDLLISKGWPPAEEETLIEESLSFALPPQLRKRRT
TAK
>Mature_322_residues
AVTGACTNKKLYLAAPRGYCAGVDRAVVTVEKALEVYGAPVYVRKQIVHNRHVVETLEGRGAIFVDELDEVPDDALVVFS
AHGVSPQVKKEATDRGLRTIDATCPLVTKVHHEAKRFANQDTQILLIGHAGHEEVEGTTGEAPENITLVQTPADVDGLAL
DRDRPVAWLSQTTLSVDETVETVDRIRDLHPQLIDPPSDDICYATQNRQHAVKQMAPQCDLVIVVGSKNSSNTGRLVEVA
LESGAKASYRVDNAGEIEETWLDGVSTIGVTSGASVPEALVQGVIDLLISKGWPPAEEETLIEESLSFALPPQLRKRRTT
AK

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=49.8381877022654, Blast_Score=307, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_PROAC (Q6AA89)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055285.1
- GeneID:   2931713
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0572
- NMPDR:   fig|267747.1.peg.562
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   PACN267747:PPA0572-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34965; Mature: 34834

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTGACTNKKLYLAAPRGYCAGVDRAVVTVEKALEVYGAPVYVRKQIVHNRHVVETLEG
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHCC
RGAIFVDELDEVPDDALVVFSAHGVSPQVKKEATDRGLRTIDATCPLVTKVHHEAKRFAN
CCCEEECCHHCCCCCEEEEEEECCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCC
QDTQILLIGHAGHEEVEGTTGEAPENITLVQTPADVDGLALDRDRPVAWLSQTTLSVDET
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEEHHHHHCCHHHH
VETVDRIRDLHPQLIDPPSDDICYATQNRQHAVKQMAPQCDLVIVVGSKNSSNTGRLVEV
HHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEE
ALESGAKASYRVDNAGEIEETWLDGVSTIGVTSGASVPEALVQGVIDLLISKGWPPAEEE
EECCCCCCEEECCCCCCHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
TLIEESLSFALPPQLRKRRTTAK
HHHHHHHCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
AVTGACTNKKLYLAAPRGYCAGVDRAVVTVEKALEVYGAPVYVRKQIVHNRHVVETLEG
CCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHCC
RGAIFVDELDEVPDDALVVFSAHGVSPQVKKEATDRGLRTIDATCPLVTKVHHEAKRFAN
CCCEEECCHHCCCCCEEEEEEECCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCC
QDTQILLIGHAGHEEVEGTTGEAPENITLVQTPADVDGLALDRDRPVAWLSQTTLSVDET
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEEHHHHHCCHHHH
VETVDRIRDLHPQLIDPPSDDICYATQNRQHAVKQMAPQCDLVIVVGSKNSSNTGRLVEV
HHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEE
ALESGAKASYRVDNAGEIEETWLDGVSTIGVTSGASVPEALVQGVIDLLISKGWPPAEEE
EECCCCCCEEECCCCCCHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
TLIEESLSFALPPQLRKRRTTAK
HHHHHHHCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA