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The map label for this gene is ksgA

Identifier: 50842010

GI number: 50842010

Start: 580160

End: 581056

Strand: Direct

Name: ksgA

Synonym: PPA0526

Alternate gene names: 50842010

Gene position: 580160-581056 (Clockwise)

Preceding gene: 50842009

Following gene: 50842011

Centisome position: 22.66

GC content: 63.43

Gene sequence:

>897_bases
GTGAGTGAAACCGGCCTGCTCAACCCGGCGTCGATCCGGCGCATTGCTGACCAGATCGGTCTGCGACCGACAAAGACTCG
CGGGCAAAATTTCGTCCACGACGCTAACACGGTGCGCCGGATAGTTTCCCTGGCGCAGGTGGGCGCCGCAGACCGAGTTA
TCGAAGTCGGCCCCGGGCTGGGTTCGCTCACCTTGGGGCTGTTGGAGACCGGCGCGGAGGTCGTTGCCATCGAGATTGAC
GAGGTGTTGGCCAACCAGCTTCCCGGTACCGTCGCTGAACGAATGCCGGGTGCTGCCGAGAGGTTGGAAGTCGTCCTGTC
CGACGCCCTTGACGTCAAGGTCATCCCCGGTGCGGAACCCACCGCCTTGGTGGCGAACTTGCCGTATAATGTTGCGGTTC
CGGTGCTGTTGCACATGCTGGCAATATGTCCGCAGTGGAGCACCGGCGTCGTCATGGTGCAGTCGGAGGTGGCCGACCGA
CTGGTAGCCGCTCCCGGCTCTAAGATCTATGGGGTTCCAAGCGCCAAACTGGCATGGTATGCCGAGGCGATTCGGGTCGG
TAACGTTCCCCCGACGGTCTTCTGGCCAGTGCCCAATGTGGATTCCGGGTTGGTCCGGATTACGAGACGGCGTCCTCCCC
ACGTCGACGGGCGGGACCCGCGCGTCACCCGTTCCCAAGTCTTCCGCGTTGTCGACGCCGCTTTCGCCTCTCGCCGCAAA
ATGCTGCGCTCGGCCCTGGCCGGGTTATGCGGTGGTTCAATGGCGGCTTCCGAGCTCATTACCGCTGCTGGAATTGACCC
GACGGCGCGTGGCGAAGCCCTCGATATTGGCGATCTGGCCCGCGTTGTAGAGGCCCTAGCCCAAGCTGGTGCTCTCAGCG
ATTCGGGTCAGGTGTGA

Upstream 100 bases:

>100_bases
CTCACACTGCACGCTTCATCGCGAAACAGCGGGGGTGGGATGAACTCCGTGTCTGTCAACAACTAACCGAGAACGCCTTT
GCCGCGTATGGAGGTGCCTG

Downstream 100 bases:

>100_bases
ACTTCTGCCGGTACGGTTAGCACATGGTGATGATGGACGATCCGTTTGACTTGCTCCGGCCGATGACCGTGACGGTGCGG
GTCAGCGCCAAGGTCAATCT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSETGLLNPASIRRIADQIGLRPTKTRGQNFVHDANTVRRIVSLAQVGAADRVIEVGPGLGSLTLGLLETGAEVVAIEID
EVLANQLPGTVAERMPGAAERLEVVLSDALDVKVIPGAEPTALVANLPYNVAVPVLLHMLAICPQWSTGVVMVQSEVADR
LVAAPGSKIYGVPSAKLAWYAEAIRVGNVPPTVFWPVPNVDSGLVRITRRRPPHVDGRDPRVTRSQVFRVVDAAFASRRK
MLRSALAGLCGGSMAASELITAAGIDPTARGEALDIGDLARVVEALAQAGALSDSGQV

Sequences:

>Translated_298_residues
MSETGLLNPASIRRIADQIGLRPTKTRGQNFVHDANTVRRIVSLAQVGAADRVIEVGPGLGSLTLGLLETGAEVVAIEID
EVLANQLPGTVAERMPGAAERLEVVLSDALDVKVIPGAEPTALVANLPYNVAVPVLLHMLAICPQWSTGVVMVQSEVADR
LVAAPGSKIYGVPSAKLAWYAEAIRVGNVPPTVFWPVPNVDSGLVRITRRRPPHVDGRDPRVTRSQVFRVVDAAFASRRK
MLRSALAGLCGGSMAASELITAAGIDPTARGEALDIGDLARVVEALAQAGALSDSGQV
>Mature_297_residues
SETGLLNPASIRRIADQIGLRPTKTRGQNFVHDANTVRRIVSLAQVGAADRVIEVGPGLGSLTLGLLETGAEVVAIEIDE
VLANQLPGTVAERMPGAAERLEVVLSDALDVKVIPGAEPTALVANLPYNVAVPVLLHMLAICPQWSTGVVMVQSEVADRL
VAAPGSKIYGVPSAKLAWYAEAIRVGNVPPTVFWPVPNVDSGLVRITRRRPPHVDGRDPRVTRSQVFRVVDAAFASRRKM
LRSALAGLCGGSMAASELITAAGIDPTARGEALDIGDLARVVEALAQAGALSDSGQV

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=284, Percent_Identity=29.9295774647887, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI156415992, Length=300, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1786236, Length=275, Percent_Identity=32.3636363636364, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25146882, Length=189, Percent_Identity=35.978835978836, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25141369, Length=308, Percent_Identity=27.5974025974026, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=285, Percent_Identity=27.3684210526316, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21358017, Length=194, Percent_Identity=31.9587628865979, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PROAC (Q6AAD7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055237.1
- ProteinModelPortal:   Q6AAD7
- SMR:   Q6AAD7
- GeneID:   2932150
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0526
- NMPDR:   fig|267747.1.peg.514
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   PACN267747:PPA0526-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31274; Mature: 31142

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 30-30 BINDING 32-32 BINDING 57-57 BINDING 78-78 BINDING 108-108 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETGLLNPASIRRIADQIGLRPTKTRGQNFVHDANTVRRIVSLAQVGAADRVIEVGPGL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
GSLTLGLLETGAEVVAIEIDEVLANQLPGTVAERMPGAAERLEVVLSDALDVKVIPGAEP
HHHHHHHHHCCCEEEEEEHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCC
TALVANLPYNVAVPVLLHMLAICPQWSTGVVMVQSEVADRLVAAPGSKIYGVPSAKLAWY
CEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCEECCCCHHHHHH
AEAIRVGNVPPTVFWPVPNVDSGLVRITRRRPPHVDGRDPRVTRSQVFRVVDAAFASRRK
HHHHHCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MLRSALAGLCGGSMAASELITAAGIDPTARGEALDIGDLARVVEALAQAGALSDSGQV
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SETGLLNPASIRRIADQIGLRPTKTRGQNFVHDANTVRRIVSLAQVGAADRVIEVGPGL
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
GSLTLGLLETGAEVVAIEIDEVLANQLPGTVAERMPGAAERLEVVLSDALDVKVIPGAEP
HHHHHHHHHCCCEEEEEEHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCC
TALVANLPYNVAVPVLLHMLAICPQWSTGVVMVQSEVADRLVAAPGSKIYGVPSAKLAWY
CEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCEECCCCHHHHHH
AEAIRVGNVPPTVFWPVPNVDSGLVRITRRRPPHVDGRDPRVTRSQVFRVVDAAFASRRK
HHHHHCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MLRSALAGLCGGSMAASELITAAGIDPTARGEALDIGDLARVVEALAQAGALSDSGQV
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA