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The map label for this gene is ispE [H]

Identifier: 50842011

GI number: 50842011

Start: 581080

End: 582051

Strand: Direct

Name: ispE [H]

Synonym: PPA0527

Alternate gene names: 50842011

Gene position: 581080-582051 (Clockwise)

Preceding gene: 50842010

Following gene: 50842012

Centisome position: 22.7

GC content: 62.55

Gene sequence:

>972_bases
ATGGTGATGATGGACGATCCGTTTGACTTGCTCCGGCCGATGACCGTGACGGTGCGGGTCAGCGCCAAGGTCAATCTGGC
GCTCGGGGTTGGTGGCCTTGCGCCCGACGGCTTTCACCCGTTGGCGACTGTCTTCGAAGCTATCTGCATTTACGATGAGG
TGGCGGTGACTCGTCGCGACGATTCGCGCATCACCCTCACCGTCTCAGGAGAGGACGCCGATCAGGTCCCTACCGACGAG
ACGAATTTGGCCTGGCGAGCTGTAGAACTCGTTCGCGAGGAATTCGCTTCAGAATGGGGGAAACGTGGCCATGGCGCCGA
CATTCACATCGATAAATCGATCCCGGTCGCTGGTGGGATGGCTGGAGGATCAGCTGACGCAGCGGGCGCCCTCATGGCAG
CGGCCGCGCTGTGTCGGCTCCCTTACTCCCCCGAGGAATTACAACCGTTGGCTGCTCAGCTTGGCTCCGACGTGCCCTTC
TGTCTCACCGGTGGAGTGGCGTTAGGGCGAGGTCGAGGAGACCGTCTTGCGCCAGTGATCTGCCGAGGACGTCATCGCTG
GGTGTTCGCGACATCGAATCAGGGACTGTCCACCCCGGCTGTCTACCGACGTTTCGACGAGTTGGGCGGCACCCCTGGGG
GAGAAACGGTGCCCAATGACCTCATCTCGGCCTTGACCCGCGGCGATCTTGATGCTGTCGCTGCCAGTTTGAGCAATGAC
TTACAGGCCGCTGCCATCGACCTTCGGCCCGAACTCGAAGAGGTGTTGACGGTTGGGCGAGAGGTGGGTGCGTTGACCGC
TTTGGTTTCTGGATCGGGGCCGACCTGCGCCTTCCTCGTCCGGGACACCGCTGGCGCCAAAAAGGTGAGCGCTGCTGTGT
CCAATCTGCCTCAGGTTCATCGCACCCGAACTGCCCGCGGTCCAGCTGCGGGAGCGCAGCTCCTGCCTGGCCCGGTGGGG
AGCTTTGCATGA

Upstream 100 bases:

>100_bases
TCGATATTGGCGATCTGGCCCGCGTTGTAGAGGCCCTAGCCCAAGCTGGTGCTCTCAGCGATTCGGGTCAGGTGTGAACT
TCTGCCGGTACGGTTAGCAC

Downstream 100 bases:

>100_bases
CGAGGCCGCTACATCAGAACGCTGTGCACCAGAATTCGCCACGGAAGGACGCGGTCGACGGCATCATTGCGGCGTGGGCT
AGGGAGCGTCCTGACTTGCC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MVMMDDPFDLLRPMTVTVRVSAKVNLALGVGGLAPDGFHPLATVFEAICIYDEVAVTRRDDSRITLTVSGEDADQVPTDE
TNLAWRAVELVREEFASEWGKRGHGADIHIDKSIPVAGGMAGGSADAAGALMAAAALCRLPYSPEELQPLAAQLGSDVPF
CLTGGVALGRGRGDRLAPVICRGRHRWVFATSNQGLSTPAVYRRFDELGGTPGGETVPNDLISALTRGDLDAVAASLSND
LQAAAIDLRPELEEVLTVGREVGALTALVSGSGPTCAFLVRDTAGAKKVSAAVSNLPQVHRTRTARGPAAGAQLLPGPVG
SFA

Sequences:

>Translated_323_residues
MVMMDDPFDLLRPMTVTVRVSAKVNLALGVGGLAPDGFHPLATVFEAICIYDEVAVTRRDDSRITLTVSGEDADQVPTDE
TNLAWRAVELVREEFASEWGKRGHGADIHIDKSIPVAGGMAGGSADAAGALMAAAALCRLPYSPEELQPLAAQLGSDVPF
CLTGGVALGRGRGDRLAPVICRGRHRWVFATSNQGLSTPAVYRRFDELGGTPGGETVPNDLISALTRGDLDAVAASLSND
LQAAAIDLRPELEEVLTVGREVGALTALVSGSGPTCAFLVRDTAGAKKVSAAVSNLPQVHRTRTARGPAAGAQLLPGPVG
SFA
>Mature_323_residues
MVMMDDPFDLLRPMTVTVRVSAKVNLALGVGGLAPDGFHPLATVFEAICIYDEVAVTRRDDSRITLTVSGEDADQVPTDE
TNLAWRAVELVREEFASEWGKRGHGADIHIDKSIPVAGGMAGGSADAAGALMAAAALCRLPYSPEELQPLAAQLGSDVPF
CLTGGVALGRGRGDRLAPVICRGRHRWVFATSNQGLSTPAVYRRFDELGGTPGGETVPNDLISALTRGDLDAVAASLSND
LQAAAIDLRPELEEVLTVGREVGALTALVSGSGPTCAFLVRDTAGAKKVSAAVSNLPQVHRTRTARGPAAGAQLLPGPVG
SFA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=266, Percent_Identity=30.8270676691729, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 33586; Mature: 33586

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMMDDPFDLLRPMTVTVRVSAKVNLALGVGGLAPDGFHPLATVFEAICIYDEVAVTRRD
CCCCCCCHHHHCCEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEECC
DSRITLTVSGEDADQVPTDETNLAWRAVELVREEFASEWGKRGHGADIHIDKSIPVAGGM
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
AGGSADAAGALMAAAALCRLPYSPEELQPLAAQLGSDVPFCLTGGVALGRGRGDRLAPVI
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCCHHE
CRGRHRWVFATSNQGLSTPAVYRRFDELGGTPGGETVPNDLISALTRGDLDAVAASLSND
ECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCC
LQAAAIDLRPELEEVLTVGREVGALTALVSGSGPTCAFLVRDTAGAKKVSAAVSNLPQVH
HHEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHCHHHH
RTRTARGPAAGAQLLPGPVGSFA
HHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVMMDDPFDLLRPMTVTVRVSAKVNLALGVGGLAPDGFHPLATVFEAICIYDEVAVTRRD
CCCCCCCHHHHCCEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEECC
DSRITLTVSGEDADQVPTDETNLAWRAVELVREEFASEWGKRGHGADIHIDKSIPVAGGM
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
AGGSADAAGALMAAAALCRLPYSPEELQPLAAQLGSDVPFCLTGGVALGRGRGDRLAPVI
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCCHHE
CRGRHRWVFATSNQGLSTPAVYRRFDELGGTPGGETVPNDLISALTRGDLDAVAASLSND
ECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCC
LQAAAIDLRPELEEVLTVGREVGALTALVSGSGPTCAFLVRDTAGAKKVSAAVSNLPQVH
HHEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHCHHHH
RTRTARGPAAGAQLLPGPVGSFA
HHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA