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The map label for this gene is dcuA [H]

Identifier: 50841586

GI number: 50841586

Start: 110409

End: 111707

Strand: Direct

Name: dcuA [H]

Synonym: PPA0095

Alternate gene names: 50841586

Gene position: 110409-111707 (Clockwise)

Preceding gene: 50841585

Following gene: 50841588

Centisome position: 4.31

GC content: 58.12

Gene sequence:

>1299_bases
ATGATGCTCTTCATTCAACTGGCCGTCGTCCTACTATTCATCTTCCTGGGGGCACGCAAGGGAGGACTTGGCATCGCCTA
TGCAGGGGGTGCCGGCGTCATCGTCCTGGGATTACTCGGCTGCAAGGTCGACCCAGGTACTGGTATTCCGTGGGACGTCA
TTGGGGTCATCATCTCAGTGATTTCTTGCGTGGCGGCGATGGAAGTAGCCGGCGGCCTAGAACTGCTGGTGATGCTCAGC
GAACGGATATTGCGGCACAACCCGAAGAGAATCACCTTCCTGGCGCCCACCGTGACGTTCTTCATGACCGTGTTGTGTGG
TACCGGACATGTCGCATTCGCCACTCTTCCCGTCATTGCGGAAGTGGCGAAGGAACAGAAGGTGAGACCCTCACGCCCAC
TATCCATCGCGGCGGTGGCCTCGCAGATCGGCATATGTGCCTCTCCGATTTCGGCGGCGATGGTGGCGATGGCGGCCATT
GTTGGCCCCTTGGGGGTTTCGTATCCGAAACTGGTCCTGGTCTCGATCGTTGGGGGTTACGTTGGCTCGATGATTGGTGC
GGTTGTTTCCTCAAAACTCGGATGCGAGTTGGAACTCGACCCGGTCTATCTACAGCGACTGGAAAAGGGCCAAATCTCGC
ATCGGGAGGCGGACAACTACAACATCAAGCCCTACGCAAGGCGTTCGCTGATAATCTTCGTGGTATCCCTGGTCGTGGTG
ATGGCTTATGCCGCTGCCATCACCGCCGTGGACAAGCCCCCGCTGCCGCGAGGCGCCGCCATCATGACGTTTATGATGAC
TGCCGCCCTCGTCATAGCCACCCTGTGTAAAGTCCCGCTTAACGAGATCACTTCCCAGGCAACCTATAAAAGTGGCACCT
CTGCTGCCATCTGCGTGATGGGGGTCGCTTGGCTAGGCAACACCTTCGTGAGTTCAAACATCGACACCATCAAAGCTGCC
GGATCGGGAGTCATCCACTCGGCACCGTGGCTGTTGTTCGTGGTGCTATTCCTGGCTGCATCGTTGCTCTACTCGCAGGC
CGCAACCACGGTTACGTTCATGCCGGTTGCTGCGGCTCTTGGGCTTCCAGCGTCGGTCCTGGTCGGATGTTTCGCGGCGG
CCTCGGCGCTGTTCCTGCTACCCACGTATCCCACCGTGGTGGCTGCGGTCGAGATGGACGATACCGGGACAACGAGGATC
GGAAAATACATCTTCAACCATTCGTTCTTGATCCCCGGCATCGTCTCGATATCGGCTGCCTGTTTGGCTTCCTACGGGGT
GATGCTCGTAGTCGGATGA

Upstream 100 bases:

>100_bases
CTTCCTGCACCCCCGGTATTCCGGACGTACCTACGAGCCGAGTGACCGCGGTCTCCCGGAGGATCCCAGGACATTCGTGT
CCGAGCGTTGCTGACACGCG

Downstream 100 bases:

>100_bases
ATGCCGCCATTCGCACAATCCGTTGAAGTACCCAGTGTGAAAGCGCGTGGGTGGGGCTCAGCCTTGGAAAAGGCTCAGCC
CCACCCACGCATTTGGGGGC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MMLFIQLAVVLLFIFLGARKGGLGIAYAGGAGVIVLGLLGCKVDPGTGIPWDVIGVIISVISCVAAMEVAGGLELLVMLS
ERILRHNPKRITFLAPTVTFFMTVLCGTGHVAFATLPVIAEVAKEQKVRPSRPLSIAAVASQIGICASPISAAMVAMAAI
VGPLGVSYPKLVLVSIVGGYVGSMIGAVVSSKLGCELELDPVYLQRLEKGQISHREADNYNIKPYARRSLIIFVVSLVVV
MAYAAAITAVDKPPLPRGAAIMTFMMTAALVIATLCKVPLNEITSQATYKSGTSAAICVMGVAWLGNTFVSSNIDTIKAA
GSGVIHSAPWLLFVVLFLAASLLYSQAATTVTFMPVAAALGLPASVLVGCFAAASALFLLPTYPTVVAAVEMDDTGTTRI
GKYIFNHSFLIPGIVSISAACLASYGVMLVVG

Sequences:

>Translated_432_residues
MMLFIQLAVVLLFIFLGARKGGLGIAYAGGAGVIVLGLLGCKVDPGTGIPWDVIGVIISVISCVAAMEVAGGLELLVMLS
ERILRHNPKRITFLAPTVTFFMTVLCGTGHVAFATLPVIAEVAKEQKVRPSRPLSIAAVASQIGICASPISAAMVAMAAI
VGPLGVSYPKLVLVSIVGGYVGSMIGAVVSSKLGCELELDPVYLQRLEKGQISHREADNYNIKPYARRSLIIFVVSLVVV
MAYAAAITAVDKPPLPRGAAIMTFMMTAALVIATLCKVPLNEITSQATYKSGTSAAICVMGVAWLGNTFVSSNIDTIKAA
GSGVIHSAPWLLFVVLFLAASLLYSQAATTVTFMPVAAALGLPASVLVGCFAAASALFLLPTYPTVVAAVEMDDTGTTRI
GKYIFNHSFLIPGIVSISAACLASYGVMLVVG
>Mature_432_residues
MMLFIQLAVVLLFIFLGARKGGLGIAYAGGAGVIVLGLLGCKVDPGTGIPWDVIGVIISVISCVAAMEVAGGLELLVMLS
ERILRHNPKRITFLAPTVTFFMTVLCGTGHVAFATLPVIAEVAKEQKVRPSRPLSIAAVASQIGICASPISAAMVAMAAI
VGPLGVSYPKLVLVSIVGGYVGSMIGAVVSSKLGCELELDPVYLQRLEKGQISHREADNYNIKPYARRSLIIFVVSLVVV
MAYAAAITAVDKPPLPRGAAIMTFMMTAALVIATLCKVPLNEITSQATYKSGTSAAICVMGVAWLGNTFVSSNIDTIKAA
GSGVIHSAPWLLFVVLFLAASLLYSQAATTVTFMPVAAALGLPASVLVGCFAAASALFLLPTYPTVVAAVEMDDTGTTRI
GKYIFNHSFLIPGIVSISAACLASYGVMLVVG

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=424, Percent_Identity=49.7641509433962, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1790565, Length=390, Percent_Identity=30.2564102564103, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 44839; Mature: 44839

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLFIQLAVVLLFIFLGARKGGLGIAYAGGAGVIVLGLLGCKVDPGTGIPWDVIGVIISV
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ISCVAAMEVAGGLELLVMLSERILRHNPKRITFLAPTVTFFMTVLCGTGHVAFATLPVIA
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHH
EVAKEQKVRPSRPLSIAAVASQIGICASPISAAMVAMAAIVGPLGVSYPKLVLVSIVGGY
HHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VGSMIGAVVSSKLGCELELDPVYLQRLEKGQISHREADNYNIKPYARRSLIIFVVSLVVV
HHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
MAYAAAITAVDKPPLPRGAAIMTFMMTAALVIATLCKVPLNEITSQATYKSGTSAAICVM
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHH
GVAWLGNTFVSSNIDTIKAAGSGVIHSAPWLLFVVLFLAASLLYSQAATTVTFMPVAAAL
HHHHHCCHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLPASVLVGCFAAASALFLLPTYPTVVAAVEMDDTGTTRIGKYIFNHSFLIPGIVSISAA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHH
CLASYGVMLVVG
HHHHCCEEEEEC
>Mature Secondary Structure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HHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11004174; 14500908; 8898907 [H]