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The map label for this gene is aspA

Identifier: 50841585

GI number: 50841585

Start: 108930

End: 110402

Strand: Direct

Name: aspA

Synonym: PPA0094

Alternate gene names: 50841585

Gene position: 108930-110402 (Clockwise)

Preceding gene: 50841581

Following gene: 50841586

Centisome position: 4.25

GC content: 53.84

Gene sequence:

>1473_bases
TTGACTGAGATCCGGATCGAAGAAGATCTTCTTGGGAAACGGGAAATCTCGAATGAGCACTACTATGGAATTCATACTTT
GCGGGCTTTAGAGAACTTCCAAATATCACGCGGATGTATGAATGACGAGCCTGACTTCATTCGTGGAATGGTTCAGGTAA
AGAAGGCAACAGCTCTGACGAATAAGCAGTTGAAGGTTCTCCCGAGTGATGTCGCTGACGCCATTGTCGCTGCATGCGAT
GCGATCTTGGATGATGGGCGCTGTATGGACCAATTCCCGACTGATTCCTTCCAAGGAGGAGCGGGAACCTCGATCAACAT
GAACACCAATGAGGTTGTCGCCAATTTGGCCCTCGAAATGTTGGGATACCCGAAAGGCACCTATGACGTCATCCACCCCA
ACGATGATGTCAATAAGTCCCAGTCGACTAACGACGCTTACCCCACCGGGTTCCGGCTCGGGGTGTACGCTTTGCTAGAG
GGGCTGAAGCGAGCCGTCGCCGACCTCGCAGACAGTTTCGATGCCAAGGCTGAGGAATTTGCCGATGTACTGAAGATGGG
GCGTACCCAATTGCAGGATGCCGTCCCCATGACTTTGGGTGAGGAGTTTGACGGGTACGCACACAACATCCGTGCTGAAC
TGGACCGGCTTGATATTGGAAGCGATCTTCTCGTAGAGGTCAACCTGGGCGGCACCGCCATCGGAACTCGGATCAACACC
CCAGACGGATATGCTGAACTTGCTGCTGAGAAACTGGCCGAGGTGACCGGATACCCGATTCGTCCCTCCACCAACCTCAT
GGAATCGTCTTACGACAACGGCGCCTACATTGCTGTCCATTCTATTATCAAACGCCTAGCAGCGAAGATCTCCAAGATCT
GCAACGATTTACGATTGCTTTCATCTGGACCGAGGGCTGGGCTCAAGGAAATCAACCTTCCAGAGATGCAGGCGGGATCG
TCCATCATGCCGGCGAAGGTGAATCCGGTCATTCCCGAGGTCGTCAATCAGATCTGCTTCAAAGTATTTGGAAACGATGT
CACCGTCTGTTTCGCTGCCGAGGCCGGTCAGCTAGAACTTAATGTGATGGAACCGGCGCTGTCGCAAGCAATGTTCGAGT
CCATCCACCTGCTGACCAATGCCTGCGACACGTTGCGTAGCAAGTGCATTGACGGGATCACCGCAAATGTGGAGCGGTGC
CGGGAGTATGTCACCAATTCCATCGGTATCGTGACCTACTTGAATGATGTGATTGGACACCATCAAGGCGATCTCATTGG
CGAGGAAGCTGCCCGTACCGGAAAGTCAGTTCGTGAGGTCGTCCTCGAACACGACCTCATCCCGGAGGAAAGGCTGGACG
AGATCATGTCGACCCAGAACTTCCTGCACCCCCGGTATTCCGGACGTACCTACGAGCCGAGTGACCGCGGTCTCCCGGAG
GATCCCAGGACATTCGTGTCCGAGCGTTGCTGA

Upstream 100 bases:

>100_bases
TCTGTTTCTTCGCCTTTAATGACCCTATTGTTACGTATGTGGTCAAAGACGGGTGTGCGAGTTTAGATGACAAAACAACA
TATATTGGTTAGGAGTAAAG

Downstream 100 bases:

>100_bases
CACGCGATGATGCTCTTCATTCAACTGGCCGTCGTCCTACTATTCATCTTCCTGGGGGCACGCAAGGGAGGACTTGGCAT
CGCCTATGCAGGGGGTGCCG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTEIRIEEDLLGKREISNEHYYGIHTLRALENFQISRGCMNDEPDFIRGMVQVKKATALTNKQLKVLPSDVADAIVAACD
AILDDGRCMDQFPTDSFQGGAGTSINMNTNEVVANLALEMLGYPKGTYDVIHPNDDVNKSQSTNDAYPTGFRLGVYALLE
GLKRAVADLADSFDAKAEEFADVLKMGRTQLQDAVPMTLGEEFDGYAHNIRAELDRLDIGSDLLVEVNLGGTAIGTRINT
PDGYAELAAEKLAEVTGYPIRPSTNLMESSYDNGAYIAVHSIIKRLAAKISKICNDLRLLSSGPRAGLKEINLPEMQAGS
SIMPAKVNPVIPEVVNQICFKVFGNDVTVCFAAEAGQLELNVMEPALSQAMFESIHLLTNACDTLRSKCIDGITANVERC
REYVTNSIGIVTYLNDVIGHHQGDLIGEEAARTGKSVREVVLEHDLIPEERLDEIMSTQNFLHPRYSGRTYEPSDRGLPE
DPRTFVSERC

Sequences:

>Translated_490_residues
MTEIRIEEDLLGKREISNEHYYGIHTLRALENFQISRGCMNDEPDFIRGMVQVKKATALTNKQLKVLPSDVADAIVAACD
AILDDGRCMDQFPTDSFQGGAGTSINMNTNEVVANLALEMLGYPKGTYDVIHPNDDVNKSQSTNDAYPTGFRLGVYALLE
GLKRAVADLADSFDAKAEEFADVLKMGRTQLQDAVPMTLGEEFDGYAHNIRAELDRLDIGSDLLVEVNLGGTAIGTRINT
PDGYAELAAEKLAEVTGYPIRPSTNLMESSYDNGAYIAVHSIIKRLAAKISKICNDLRLLSSGPRAGLKEINLPEMQAGS
SIMPAKVNPVIPEVVNQICFKVFGNDVTVCFAAEAGQLELNVMEPALSQAMFESIHLLTNACDTLRSKCIDGITANVERC
REYVTNSIGIVTYLNDVIGHHQGDLIGEEAARTGKSVREVVLEHDLIPEERLDEIMSTQNFLHPRYSGRTYEPSDRGLPE
DPRTFVSERC
>Mature_489_residues
TEIRIEEDLLGKREISNEHYYGIHTLRALENFQISRGCMNDEPDFIRGMVQVKKATALTNKQLKVLPSDVADAIVAACDA
ILDDGRCMDQFPTDSFQGGAGTSINMNTNEVVANLALEMLGYPKGTYDVIHPNDDVNKSQSTNDAYPTGFRLGVYALLEG
LKRAVADLADSFDAKAEEFADVLKMGRTQLQDAVPMTLGEEFDGYAHNIRAELDRLDIGSDLLVEVNLGGTAIGTRINTP
DGYAELAAEKLAEVTGYPIRPSTNLMESSYDNGAYIAVHSIIKRLAAKISKICNDLRLLSSGPRAGLKEINLPEMQAGSS
IMPAKVNPVIPEVVNQICFKVFGNDVTVCFAAEAGQLELNVMEPALSQAMFESIHLLTNACDTLRSKCIDGITANVERCR
EYVTNSIGIVTYLNDVIGHHQGDLIGEEAARTGKSVREVVLEHDLIPEERLDEIMSTQNFLHPRYSGRTYEPSDRGLPED
PRTFVSERC

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=467, Percent_Identity=37.9014989293362, Blast_Score=317, Evalue=2e-86,
Organism=Escherichia coli, GI87082375, Length=468, Percent_Identity=64.3162393162393, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1787896, Length=470, Percent_Identity=36.8085106382979, Blast_Score=308, Evalue=8e-85,
Organism=Caenorhabditis elegans, GI17553882, Length=470, Percent_Identity=37.2340425531915, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI32565146, Length=331, Percent_Identity=36.2537764350453, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324993, Length=466, Percent_Identity=36.0515021459227, Blast_Score=310, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=38.5775862068966, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=38.5775862068966, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=39.7849462365591, Blast_Score=331, Evalue=8e-91,
Organism=Drosophila melanogaster, GI78710009, Length=465, Percent_Identity=39.1397849462366, Blast_Score=324, Evalue=6e-89,
Organism=Drosophila melanogaster, GI24583245, Length=467, Percent_Identity=34.9036402569593, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 53799; Mature: 53667

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIRIEEDLLGKREISNEHYYGIHTLRALENFQISRGCMNDEPDFIRGMVQVKKATALT
CCCEECHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
NKQLKVLPSDVADAIVAACDAILDDGRCMDQFPTDSFQGGAGTSINMNTNEVVANLALEM
CCHHEECCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHH
LGYPKGTYDVIHPNDDVNKSQSTNDAYPTGFRLGVYALLEGLKRAVADLADSFDAKAEEF
HCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ADVLKMGRTQLQDAVPMTLGEEFDGYAHNIRAELDRLDIGSDLLVEVNLGGTAIGTRINT
HHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
PDGYAELAAEKLAEVTGYPIRPSTNLMESSYDNGAYIAVHSIIKRLAAKISKICNDLRLL
CCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLKEINLPEMQAGSSIMPAKVNPVIPEVVNQICFKVFGNDVTVCFAAEAGQLEL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
NVMEPALSQAMFESIHLLTNACDTLRSKCIDGITANVERCREYVTNSIGIVTYLNDVIGH
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCC
HQGDLIGEEAARTGKSVREVVLEHDLIPEERLDEIMSTQNFLHPRYSGRTYEPSDRGLPE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DPRTFVSERC
CHHHHHHCCC
>Mature Secondary Structure 
TEIRIEEDLLGKREISNEHYYGIHTLRALENFQISRGCMNDEPDFIRGMVQVKKATALT
CCEECHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
NKQLKVLPSDVADAIVAACDAILDDGRCMDQFPTDSFQGGAGTSINMNTNEVVANLALEM
CCHHEECCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHH
LGYPKGTYDVIHPNDDVNKSQSTNDAYPTGFRLGVYALLEGLKRAVADLADSFDAKAEEF
HCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ADVLKMGRTQLQDAVPMTLGEEFDGYAHNIRAELDRLDIGSDLLVEVNLGGTAIGTRINT
HHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
PDGYAELAAEKLAEVTGYPIRPSTNLMESSYDNGAYIAVHSIIKRLAAKISKICNDLRLL
CCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLKEINLPEMQAGSSIMPAKVNPVIPEVVNQICFKVFGNDVTVCFAAEAGQLEL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
NVMEPALSQAMFESIHLLTNACDTLRSKCIDGITANVERCREYVTNSIGIVTYLNDVIGH
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCC
HQGDLIGEEAARTGKSVREVVLEHDLIPEERLDEIMSTQNFLHPRYSGRTYEPSDRGLPE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DPRTFVSERC
CHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7789816; 12948626 [H]