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The map label for this gene is pfkA

Identifier: 50841581

GI number: 50841581

Start: 102809

End: 104017

Strand: Direct

Name: pfkA

Synonym: PPA0090

Alternate gene names: 50841581

Gene position: 102809-104017 (Clockwise)

Preceding gene: 50841580

Following gene: 50841585

Centisome position: 4.02

GC content: 61.21

Gene sequence:

>1209_bases
ATGATTGCCACAATGGACCGCAGTGTTGTCGGCGCCATCCATGGAAGGATGGGTACCACCCGACACAGATCAGGAGTCGT
CATGTCCAAAAAGAAGAAGGTCGGCATCCTCACCGCAGGCGGTGATTGCCCCGGGCTCAACGCCGCTATCCGCGGATTTG
GCAAGGCTGCCATCCGCCAGCACGACATGGAGCTCATCGGTATTCAGGACGGCTTTCTTGGATTGGCAGGGAACCGCACC
ATCTCCCTTGACCCGCGTGCCCTCTCAGGCATCTTGACGGTCGGCGGGACCATCCTGGGAACTAGCCGTGACAAGGTCAA
TCACATGATTATCGACGGCGAGGAACGGGATATGGTCCCCACCATCGTCGAGAATTACGAGAAGCTGGGACTTGACGCTT
TGGTGACTTTGGGTGGCGGTGGCACCGCCAAGAACGCGTACAAGCTCGCTAAGGCCGGGCTGAACGTCCTGCATCTGCCC
AAGACGATTGACAACGACATCGTCGGAACCGACGACTCCTTCGGGTTCTCGACAGCACTGGAGATTGCCACGGAGGCCAT
CGACCGGCTGCACTCCACCGCCCATTCCCACCACCGCATCATCCTCGCTGAGATCATGGGCCACCGTGCCGGCTGGCTGG
CCCTCGGCTCGGGCATCGCTGGTGGCGCTGACGTCATCCTGCTGCCCGAGGTGCCCTACAAGCTGGACTCCATCGTCCAG
GCGGTGGAAAACCGGCAAAAGCAGGGGTTGAATTTCTCGGTCATCGCGGTGGCTGAGGGAGCTCGCGATGAGGCTGATTC
GGCCGCGATGGCTGGAGCTCAGGCCCTCGTTGACGCTTCCAAGACCTCCGAGACAAAGGCGGCTGCCAAGAAGGCCAAGA
AGGACCTTGAGCATTCCATGCGTGACAACACCCTCAAATTGGCTACTCAGCTTGAGGAGGCCACCGGGCTGGAATCGCGA
GTCTCGATCCTCGGCTATGTGCAGCGCGGTGGTATCCCGTGCGCCCACGACCGTCTGCTCGCGACCCGGCTGGGAACCGT
GGGTGCCAAGCTCGTTGACGACGGCGAGTTCGGCATCATGGTCGCCGCTCAGGGCGGAGACGCCACGACCGTTCCGTTGA
AAGATGTCGCTGGCAAGGTTAAATACGTACCTGCCGACCACCCATGGGTTAAGGCGGCCCGTCAGGTCGGAACCGGTCTA
GGCGACTGA

Upstream 100 bases:

>100_bases
CGGGTTATCTGACCGAGGCCCCGTCTCTCCGGCGCTGTGGCAATCCTGCCGGGGAGGACGCCAGCTGCCACGACGAATGG
CAGTCGTCACAGTGACGCAC

Downstream 100 bases:

>100_bases
GGTTCTGCCCCTAGCCCCTCTCAAGGGCATCCGTCACTCAGGTGTGTATCACACACCGGACTCCGGGGTTAGCGTCGATA
GTCGCGGCTTGCCCCGTCAC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 3; Phosphohexokinase 3 [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MIATMDRSVVGAIHGRMGTTRHRSGVVMSKKKKVGILTAGGDCPGLNAAIRGFGKAAIRQHDMELIGIQDGFLGLAGNRT
ISLDPRALSGILTVGGTILGTSRDKVNHMIIDGEERDMVPTIVENYEKLGLDALVTLGGGGTAKNAYKLAKAGLNVLHLP
KTIDNDIVGTDDSFGFSTALEIATEAIDRLHSTAHSHHRIILAEIMGHRAGWLALGSGIAGGADVILLPEVPYKLDSIVQ
AVENRQKQGLNFSVIAVAEGARDEADSAAMAGAQALVDASKTSETKAAAKKAKKDLEHSMRDNTLKLATQLEEATGLESR
VSILGYVQRGGIPCAHDRLLATRLGTVGAKLVDDGEFGIMVAAQGGDATTVPLKDVAGKVKYVPADHPWVKAARQVGTGL
GD

Sequences:

>Translated_402_residues
MIATMDRSVVGAIHGRMGTTRHRSGVVMSKKKKVGILTAGGDCPGLNAAIRGFGKAAIRQHDMELIGIQDGFLGLAGNRT
ISLDPRALSGILTVGGTILGTSRDKVNHMIIDGEERDMVPTIVENYEKLGLDALVTLGGGGTAKNAYKLAKAGLNVLHLP
KTIDNDIVGTDDSFGFSTALEIATEAIDRLHSTAHSHHRIILAEIMGHRAGWLALGSGIAGGADVILLPEVPYKLDSIVQ
AVENRQKQGLNFSVIAVAEGARDEADSAAMAGAQALVDASKTSETKAAAKKAKKDLEHSMRDNTLKLATQLEEATGLESR
VSILGYVQRGGIPCAHDRLLATRLGTVGAKLVDDGEFGIMVAAQGGDATTVPLKDVAGKVKYVPADHPWVKAARQVGTGL
GD
>Mature_402_residues
MIATMDRSVVGAIHGRMGTTRHRSGVVMSKKKKVGILTAGGDCPGLNAAIRGFGKAAIRQHDMELIGIQDGFLGLAGNRT
ISLDPRALSGILTVGGTILGTSRDKVNHMIIDGEERDMVPTIVENYEKLGLDALVTLGGGGTAKNAYKLAKAGLNVLHLP
KTIDNDIVGTDDSFGFSTALEIATEAIDRLHSTAHSHHRIILAEIMGHRAGWLALGSGIAGGADVILLPEVPYKLDSIVQ
AVENRQKQGLNFSVIAVAEGARDEADSAAMAGAQALVDASKTSETKAAAKKAKKDLEHSMRDNTLKLATQLEEATGLESR
VSILGYVQRGGIPCAHDRLLATRLGTVGAKLVDDGEFGIMVAAQGGDATTVPLKDVAGKVKYVPADHPWVKAARQVGTGL
GD

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=344, Percent_Identity=33.4302325581395, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI266453768, Length=398, Percent_Identity=27.8894472361809, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI266453748, Length=398, Percent_Identity=27.8894472361809, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4505749, Length=398, Percent_Identity=27.8894472361809, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI266453619, Length=398, Percent_Identity=27.8894472361809, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI48762920, Length=342, Percent_Identity=29.5321637426901, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1790350, Length=365, Percent_Identity=34.5205479452055, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25147584, Length=398, Percent_Identity=27.6381909547739, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17558788, Length=352, Percent_Identity=29.2613636363636, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI72003601, Length=323, Percent_Identity=25.3869969040248, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321679, Length=356, Percent_Identity=31.4606741573034, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323861, Length=346, Percent_Identity=28.3236994219653, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24652337, Length=406, Percent_Identity=29.064039408867, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI17647809, Length=405, Percent_Identity=29.1358024691358, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28573326, Length=406, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 42079; Mature: 42079

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATMDRSVVGAIHGRMGTTRHRSGVVMSKKKKVGILTAGGDCPGLNAAIRGFGKAAIRQ
CCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
HDMELIGIQDGFLGLAGNRTISLDPRALSGILTVGGTILGTSRDKVNHMIIDGEERDMVP
CCCEEEEECCCCEEECCCCEEEECHHHHHHHHHHCCEEEECCHHHCCEEEECCCCCCCCH
TIVENYEKLGLDALVTLGGGGTAKNAYKLAKAGLNVLHLPKTIDNDIVGTDDSFGFSTAL
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHH
EIATEAIDRLHSTAHSHHRIILAEIMGHRAGWLALGSGIAGGADVILLPEVPYKLDSIVQ
HHHHHHHHHHHHHHHHCCEEEEHHHHCCCCCEEEECCCCCCCCCEEEECCCCHHHHHHHH
AVENRQKQGLNFSVIAVAEGARDEADSAAMAGAQALVDASKTSETKAAAKKAKKDLEHSM
HHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RDNTLKLATQLEEATGLESRVSILGYVQRGGIPCAHDRLLATRLGTVGAKLVDDGEFGIM
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCEEEE
VAAQGGDATTVPLKDVAGKVKYVPADHPWVKAARQVGTGLGD
EEECCCCCEECCHHHHCCCEEECCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIATMDRSVVGAIHGRMGTTRHRSGVVMSKKKKVGILTAGGDCPGLNAAIRGFGKAAIRQ
CCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
HDMELIGIQDGFLGLAGNRTISLDPRALSGILTVGGTILGTSRDKVNHMIIDGEERDMVP
CCCEEEEECCCCEEECCCCEEEECHHHHHHHHHHCCEEEECCHHHCCEEEECCCCCCCCH
TIVENYEKLGLDALVTLGGGGTAKNAYKLAKAGLNVLHLPKTIDNDIVGTDDSFGFSTAL
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHH
EIATEAIDRLHSTAHSHHRIILAEIMGHRAGWLALGSGIAGGADVILLPEVPYKLDSIVQ
HHHHHHHHHHHHHHHHCCEEEEHHHHCCCCCEEEECCCCCCCCCEEEECCCCHHHHHHHH
AVENRQKQGLNFSVIAVAEGARDEADSAAMAGAQALVDASKTSETKAAAKKAKKDLEHSM
HHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RDNTLKLATQLEEATGLESRVSILGYVQRGGIPCAHDRLLATRLGTVGAKLVDDGEFGIM
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCEEEE
VAAQGGDATTVPLKDVAGKVKYVPADHPWVKAARQVGTGLGD
EEECCCCCEECCHHHHCCCEEECCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]