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The map label for this gene is gyrB

Identifier: 50841503

GI number: 50841503

Start: 9733

End: 11805

Strand: Direct

Name: gyrB

Synonym: PPA0009

Alternate gene names: 50841503

Gene position: 9733-11805 (Clockwise)

Preceding gene: 50841502

Following gene: 50841504

Centisome position: 0.38

GC content: 59.86

Gene sequence:

>2073_bases
GTGAGCCAGCCCGAGATGCCCGAGTCCGTGACCGACGGGGACACCCCCGAGGACCTTCTCATTGATGCTACCGATCATCC
TGATGGTCTGGACTCAGAGCTTCCCCCGACGGCCGTCGACGGTTCCTACGATGCCGGTCAAATCACCGTCTTAGAAGGCT
TGGAGGCCGTTCGTAAGCGTCCCGGCATGTATATCGGCTCTACTGGGGAGCGCGGGTTGCATCACTGCGTCTATGAGATT
GTCGATAACTCCGTCGACGAGGCGATGGCCGGTTATTGCGACACCATCCAGGTTGAGTTACTCGACGATAACTGGTGCCA
GGTTACCGATAACGGGCGAGGGATTCCCGTCAAGGAGCACCCCGTCGAACACATCCCTACCGTCACTTTGGTACTGACCA
AGCTGCACGCCGGCGGTAAGTTCGGCAACGGCGGTTACAAGGTCTCCGGTGGTCTACACGGCGTCGGCTCCTCGGTTGTT
AATGCTCTAGCGGCCCACTTCATCGTCGAGGTCCGACGAGACGGCTACCACTGGCGTCAGTCCTTCAGCCTCGGTGAGCC
TGACGGTCCGCTGGAGCAGCTTGAGGCACTCGGCCCCGACGATTACGAAGGCACCACGGTGCGTTTCCAGCCCTCCGACG
AGATCTTCGATGCCATCGTTTTCGACTACCAAACCTTGTCGAACCGTTTCCGTGAAATGGCCTTCCTTAATAAGGGTCTG
CGTATCGTGCTCACCGACCGCCGTGCCGACCACGCTGATGAGGACGGCAACCCGGTTCACGAGGACTTCCTCTATAAGGA
GGGGCTTAAGGACTACGTCCGATTCCTCACGGGGACCCGCGACGTCATCAACCCCACCATCATCTCCTTGGAGTCCCACC
GTCCCGAGGAGGGTATGGGTTTGGAGATTGCGATGCAATGGAACACCTCGTACGCCACCAGCGTCCACACTTTCGCCAAC
GCCATCAACACCATCGAGGGCGGAGCTCACGAGGAAGGCTTCCGTGCCGCTCTCACCAACACCGTTAATCGGTGGGGTGA
GACTTGGGGACTCATCAAGAAGCGTGAGGATCGCGTTTCCGGTGACGACATCCGCGAGGGCCTGACAGCCGTCGTCTCCA
TCAAGCTCACCGAGCCGCAGTTCGAGGGCCAGACAAAGACCAAACTCGGCAACGCCGAGGCCAAATCTTTCGTCCAAAAA
GCCCTTAATGAGAAGCTCTCGGACTGGTTCGAGGAGAATCCCAATGAGGGCAAGAGCATCGTCCGAAAGTCACAAGCCGC
TGCCCAGGCTCGCGTAGCCGCTCGCAAGGCCCGCGATCTGGCCCGTAACCGGAAAGGCCTGCTGGGGTCGACGTCACTGC
CCGGCAAACTCAAGGACTGCTCCTCAACCAGCCCGGAAGAGTGCGAGATCTTCATTGTGGAGGGCGACTCCGCTGGTGGT
TCGGCCTCTCAGGGCCGTGATCCACGTATTCAGGCGATCCTGCCGTTGCGAGGAAAGATCCTCAATGTTGAAAAAGCCCG
CCTCGACCGCGCGTTGGGTTCGGAAACTATTGAGTCGATCATTAACGCGTTAGGAACCGGCGTCCACGAGGACTTCGATC
TGGACAAACTGCGTTACCACAAGATCATCCTCATGGCGGACGCCGACGTTGACGGCGCTCATATCCGCACCCTGCTACTG
ACCCTGCTGTTCCGGTTCATGCGTCCGGTCATTGACGCTGGCCACGTCTACCTGGCACAGCCCCCGCTGTTCCGACTGCG
GTGGACGAACTCCCCGCACGAGCTCGCCTACTCCGACGAAGAGCGCGACCTGCTGCGCGAAGACGGTCTGACCCACGGCA
AGAAGCTGCCCCAAGTTAACCCGATCCAGCGGTACAAAGGTCTGGGCGAGATGGACGCCGAAGACCTGTGGGACACCACC
ATGGACCCCGACAAGCGTCTGCTGCTCCAGGTGACCCTTGAAGACGCCGCGCTGGCCGACCAGATGTTCTCCATCCTCAT
GGGTGAGGACGTCGAACAGCGCCGATCGTTCATTCAGCGCAATGCCAAGGACGTCCGCTTCCTCGACATCTGA

Upstream 100 bases:

>100_bases
TATCGGACCGTTGGGCTCCCCGCTGGTTCATGACATCCGACGGGGACGCCAGACCCGGAGGTCACCGTGACCTCTATAGA
GACACAGGAGCGCATTGCCC

Downstream 100 bases:

>100_bases
GCACCCATTCCCTCATCCCCTGATTATTCAAAGGACACTCCATGGCTGACGACGAGAAGCCCGACGAGCAGAACCAGGCC
GACCGTCAAGGATTGGTGAC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MSQPEMPESVTDGDTPEDLLIDATDHPDGLDSELPPTAVDGSYDAGQITVLEGLEAVRKRPGMYIGSTGERGLHHCVYEI
VDNSVDEAMAGYCDTIQVELLDDNWCQVTDNGRGIPVKEHPVEHIPTVTLVLTKLHAGGKFGNGGYKVSGGLHGVGSSVV
NALAAHFIVEVRRDGYHWRQSFSLGEPDGPLEQLEALGPDDYEGTTVRFQPSDEIFDAIVFDYQTLSNRFREMAFLNKGL
RIVLTDRRADHADEDGNPVHEDFLYKEGLKDYVRFLTGTRDVINPTIISLESHRPEEGMGLEIAMQWNTSYATSVHTFAN
AINTIEGGAHEEGFRAALTNTVNRWGETWGLIKKREDRVSGDDIREGLTAVVSIKLTEPQFEGQTKTKLGNAEAKSFVQK
ALNEKLSDWFEENPNEGKSIVRKSQAAAQARVAARKARDLARNRKGLLGSTSLPGKLKDCSSTSPEECEIFIVEGDSAGG
SASQGRDPRIQAILPLRGKILNVEKARLDRALGSETIESIINALGTGVHEDFDLDKLRYHKIILMADADVDGAHIRTLLL
TLLFRFMRPVIDAGHVYLAQPPLFRLRWTNSPHELAYSDEERDLLREDGLTHGKKLPQVNPIQRYKGLGEMDAEDLWDTT
MDPDKRLLLQVTLEDAALADQMFSILMGEDVEQRRSFIQRNAKDVRFLDI

Sequences:

>Translated_690_residues
MSQPEMPESVTDGDTPEDLLIDATDHPDGLDSELPPTAVDGSYDAGQITVLEGLEAVRKRPGMYIGSTGERGLHHCVYEI
VDNSVDEAMAGYCDTIQVELLDDNWCQVTDNGRGIPVKEHPVEHIPTVTLVLTKLHAGGKFGNGGYKVSGGLHGVGSSVV
NALAAHFIVEVRRDGYHWRQSFSLGEPDGPLEQLEALGPDDYEGTTVRFQPSDEIFDAIVFDYQTLSNRFREMAFLNKGL
RIVLTDRRADHADEDGNPVHEDFLYKEGLKDYVRFLTGTRDVINPTIISLESHRPEEGMGLEIAMQWNTSYATSVHTFAN
AINTIEGGAHEEGFRAALTNTVNRWGETWGLIKKREDRVSGDDIREGLTAVVSIKLTEPQFEGQTKTKLGNAEAKSFVQK
ALNEKLSDWFEENPNEGKSIVRKSQAAAQARVAARKARDLARNRKGLLGSTSLPGKLKDCSSTSPEECEIFIVEGDSAGG
SASQGRDPRIQAILPLRGKILNVEKARLDRALGSETIESIINALGTGVHEDFDLDKLRYHKIILMADADVDGAHIRTLLL
TLLFRFMRPVIDAGHVYLAQPPLFRLRWTNSPHELAYSDEERDLLREDGLTHGKKLPQVNPIQRYKGLGEMDAEDLWDTT
MDPDKRLLLQVTLEDAALADQMFSILMGEDVEQRRSFIQRNAKDVRFLDI
>Mature_689_residues
SQPEMPESVTDGDTPEDLLIDATDHPDGLDSELPPTAVDGSYDAGQITVLEGLEAVRKRPGMYIGSTGERGLHHCVYEIV
DNSVDEAMAGYCDTIQVELLDDNWCQVTDNGRGIPVKEHPVEHIPTVTLVLTKLHAGGKFGNGGYKVSGGLHGVGSSVVN
ALAAHFIVEVRRDGYHWRQSFSLGEPDGPLEQLEALGPDDYEGTTVRFQPSDEIFDAIVFDYQTLSNRFREMAFLNKGLR
IVLTDRRADHADEDGNPVHEDFLYKEGLKDYVRFLTGTRDVINPTIISLESHRPEEGMGLEIAMQWNTSYATSVHTFANA
INTIEGGAHEEGFRAALTNTVNRWGETWGLIKKREDRVSGDDIREGLTAVVSIKLTEPQFEGQTKTKLGNAEAKSFVQKA
LNEKLSDWFEENPNEGKSIVRKSQAAAQARVAARKARDLARNRKGLLGSTSLPGKLKDCSSTSPEECEIFIVEGDSAGGS
ASQGRDPRIQAILPLRGKILNVEKARLDRALGSETIESIINALGTGVHEDFDLDKLRYHKIILMADADVDGAHIRTLLLT
LLFRFMRPVIDAGHVYLAQPPLFRLRWTNSPHELAYSDEERDLLREDGLTHGKKLPQVNPIQRYKGLGEMDAEDLWDTTM
DPDKRLLLQVTLEDAALADQMFSILMGEDVEQRRSFIQRNAKDVRFLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=683, Percent_Identity=24.1581259150805, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI19913406, Length=628, Percent_Identity=24.8407643312102, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI48994957, Length=552, Percent_Identity=48.731884057971, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=37.6923076923077, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17535065, Length=565, Percent_Identity=23.5398230088496, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=565, Percent_Identity=24.9557522123894, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324241, Length=684, Percent_Identity=23.3918128654971, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17136538, Length=661, Percent_Identity=24.6596066565809, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 76653; Mature: 76522

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPEMPESVTDGDTPEDLLIDATDHPDGLDSELPPTAVDGSYDAGQITVLEGLEAVRKR
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PGMYIGSTGERGLHHCVYEIVDNSVDEAMAGYCDTIQVELLDDNWCQVTDNGRGIPVKEH
CCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCC
PVEHIPTVTLVLTKLHAGGKFGNGGYKVSGGLHGVGSSVVNALAAHFIVEVRRDGYHWRQ
CHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
SFSLGEPDGPLEQLEALGPDDYEGTTVRFQPSDEIFDAIVFDYQTLSNRFREMAFLNKGL
CCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIVLTDRRADHADEDGNPVHEDFLYKEGLKDYVRFLTGTRDVINPTIISLESHRPEEGMG
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCC
LEIAMQWNTSYATSVHTFANAINTIEGGAHEEGFRAALTNTVNRWGETWGLIKKREDRVS
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDDIREGLTAVVSIKLTEPQFEGQTKTKLGNAEAKSFVQKALNEKLSDWFEENPNEGKSI
HHHHHHCCEEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VRKSQAAAQARVAARKARDLARNRKGLLGSTSLPGKLKDCSSTSPEECEIFIVEGDSAGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
SASQGRDPRIQAILPLRGKILNVEKARLDRALGSETIESIINALGTGVHEDFDLDKLRYH
CCCCCCCCCEEEEECCCCEEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCEEEE
KIILMADADVDGAHIRTLLLTLLFRFMRPVIDAGHVYLAQPPLFRLRWTNSPHELAYSDE
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCCCCCH
ERDLLREDGLTHGKKLPQVNPIQRYKGLGEMDAEDLWDTTMDPDKRLLLQVTLEDAALAD
HHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEEEHHHHHHHH
QMFSILMGEDVEQRRSFIQRNAKDVRFLDI
HHHHHHHCCCHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
SQPEMPESVTDGDTPEDLLIDATDHPDGLDSELPPTAVDGSYDAGQITVLEGLEAVRKR
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PGMYIGSTGERGLHHCVYEIVDNSVDEAMAGYCDTIQVELLDDNWCQVTDNGRGIPVKEH
CCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCC
PVEHIPTVTLVLTKLHAGGKFGNGGYKVSGGLHGVGSSVVNALAAHFIVEVRRDGYHWRQ
CHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
SFSLGEPDGPLEQLEALGPDDYEGTTVRFQPSDEIFDAIVFDYQTLSNRFREMAFLNKGL
CCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIVLTDRRADHADEDGNPVHEDFLYKEGLKDYVRFLTGTRDVINPTIISLESHRPEEGMG
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCC
LEIAMQWNTSYATSVHTFANAINTIEGGAHEEGFRAALTNTVNRWGETWGLIKKREDRVS
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDDIREGLTAVVSIKLTEPQFEGQTKTKLGNAEAKSFVQKALNEKLSDWFEENPNEGKSI
HHHHHHCCEEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VRKSQAAAQARVAARKARDLARNRKGLLGSTSLPGKLKDCSSTSPEECEIFIVEGDSAGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
SASQGRDPRIQAILPLRGKILNVEKARLDRALGSETIESIINALGTGVHEDFDLDKLRYH
CCCCCCCCCEEEEECCCCEEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCEEEE
KIILMADADVDGAHIRTLLLTLLFRFMRPVIDAGHVYLAQPPLFRLRWTNSPHELAYSDE
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCCCCCH
ERDLLREDGLTHGKKLPQVNPIQRYKGLGEMDAEDLWDTTMDPDKRLLLQVTLEDAALAD
HHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEEEHHHHHHHH
QMFSILMGEDVEQRRSFIQRNAKDVRFLDI
HHHHHHHCCCHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8392138 [H]