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The map label for this gene is dnaN [H]

Identifier: 161621802

GI number: 161621802

Start: 2219

End: 3379

Strand: Direct

Name: dnaN [H]

Synonym: PPA0002

Alternate gene names: 161621802

Gene position: 2219-3379 (Clockwise)

Preceding gene: 50841497

Following gene: 50841499

Centisome position: 0.09

GC content: 58.48

Gene sequence:

>1161_bases
GTGGAAATCCGTCTGGAACGCGATGTCATGGCCGAAGCTGTCGCCTGGGCTGCTCGCAGTCTGCCGAACCGGCCGACAGT
GCCGATTTTGGCTGGACTGCTCGTGCGAGCTGAGGGCGACTCGGTGGTGATGTCGGCCTCCGACAATGAGACCAGCGCAC
AAATTACCCTTTCCGCTCAGGTTGACGAGCCGGGGGAATCTCTTGTCTCTGGCAAGCTGCTTGCTGATATTGCTCGTAGC
TTGCCTAACAAGCCGGTTCAGATCACGACGGACCCCGCCAAGATGGATCTGGTGTGCGGATCTGCTCGCTTTACCCTTCA
GGCCCTACCTGTTGATGAGTATCCTGATCTGCCGCAAATGCCTGCAGCAACGGGCACTGTTGACGCCAGTGTTTTCTCCA
GGGCTGTCGCTCAGGTCGTCGTGGCTGCTGGTCGTGATGAGCTATTGCCGGTCTTTACGGGTGTGCGCGTCGAAATCAAC
GGGGAGACCCTCTCGCTGCTGGCTACAGACCGTTACCGGATGGCTCTCAAGGAAATCACCTGGAATCCCAGCGCCACCGA
CGCTGAGGCTACTGCTTTGGTGCCGGCTAAGGTCATCAATGAAACCGCGCGCTCGATGACCTCTGGCGAGCACGTCACCA
TGAACCTTTCTTCGGGCGACAGTGGTGAAGGTCTTGTCGGTTTTGAAGGTGACGGGGCCAACGGTGTGCGTCGCATGACC
ACTCGCTTGCTAAGTGGGGAATTCCCTAAAGTGCGTCACCTCATGGACATCAAGGCCACTCGTTCGGTGCGGGCTCGTAC
CGATGAGCTCATCAACTCGGTGCGTCGCGTTTCCCTCGTTGCTGAGCGCAACACTCCGCTGCGGATGGTCATTAATGACG
ACTCGGTCGCCTTGTCAGCGGCCACGGGCGACCAGGCTCAAGCTTCTGAGGCCATTGAGGCTGTTGTTACAAACCACGTT
GACGGGGAGCCCACTATTACCGCGGCAGGGTTCAACCCGCATTATCTGTCTGACGCCCTTGGTGCCCTGGATACCCCCTA
CGTCCACTTCTCTTTCACCGCTCCTGGCAAGCCTTGCCTGGTGACTGGTCTCAATGACTTCGACGGCAAGCCGGAGACGG
ATTACCGTCACGTCATCATGCTGATGAGATTGCCCTCCTGA

Upstream 100 bases:

>100_bases
TGACGTCGACATCACCATGGGCGTGCCAACGGTCGGCAAGCATGGATATGATCAGCATGCAGGCACCATGGAGTGCGAGA
CGCCAAATCAAGGGGACGCA

Downstream 100 bases:

>100_bases
GGTAGCTTCGCGTCACGAAGAGTCTTGCTCGATAGCCCCATAAAGCATACTTGTATTGGGCTATAGCACTGCCCTTGATA
GGCAAGTGCCATGGGGTGCC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MEIRLERDVMAEAVAWAARSLPNRPTVPILAGLLVRAEGDSVVMSASDNETSAQITLSAQVDEPGESLVSGKLLADIARS
LPNKPVQITTDPAKMDLVCGSARFTLQALPVDEYPDLPQMPAATGTVDASVFSRAVAQVVVAAGRDELLPVFTGVRVEIN
GETLSLLATDRYRMALKEITWNPSATDAEATALVPAKVINETARSMTSGEHVTMNLSSGDSGEGLVGFEGDGANGVRRMT
TRLLSGEFPKVRHLMDIKATRSVRARTDELINSVRRVSLVAERNTPLRMVINDDSVALSAATGDQAQASEAIEAVVTNHV
DGEPTITAAGFNPHYLSDALGALDTPYVHFSFTAPGKPCLVTGLNDFDGKPETDYRHVIMLMRLPS

Sequences:

>Translated_386_residues
MEIRLERDVMAEAVAWAARSLPNRPTVPILAGLLVRAEGDSVVMSASDNETSAQITLSAQVDEPGESLVSGKLLADIARS
LPNKPVQITTDPAKMDLVCGSARFTLQALPVDEYPDLPQMPAATGTVDASVFSRAVAQVVVAAGRDELLPVFTGVRVEIN
GETLSLLATDRYRMALKEITWNPSATDAEATALVPAKVINETARSMTSGEHVTMNLSSGDSGEGLVGFEGDGANGVRRMT
TRLLSGEFPKVRHLMDIKATRSVRARTDELINSVRRVSLVAERNTPLRMVINDDSVALSAATGDQAQASEAIEAVVTNHV
DGEPTITAAGFNPHYLSDALGALDTPYVHFSFTAPGKPCLVTGLNDFDGKPETDYRHVIMLMRLPS
>Mature_386_residues
MEIRLERDVMAEAVAWAARSLPNRPTVPILAGLLVRAEGDSVVMSASDNETSAQITLSAQVDEPGESLVSGKLLADIARS
LPNKPVQITTDPAKMDLVCGSARFTLQALPVDEYPDLPQMPAATGTVDASVFSRAVAQVVVAAGRDELLPVFTGVRVEIN
GETLSLLATDRYRMALKEITWNPSATDAEATALVPAKVINETARSMTSGEHVTMNLSSGDSGEGLVGFEGDGANGVRRMT
TRLLSGEFPKVRHLMDIKATRSVRARTDELINSVRRVSLVAERNTPLRMVINDDSVALSAATGDQAQASEAIEAVVTNHV
DGEPTITAAGFNPHYLSDALGALDTPYVHFSFTAPGKPCLVTGLNDFDGKPETDYRHVIMLMRLPS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=359, Percent_Identity=23.6768802228412, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41220; Mature: 41220

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRLERDVMAEAVAWAARSLPNRPTVPILAGLLVRAEGDSVVMSASDNETSAQITLSAQ
CCEEEHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEECCCEEEEECCCCCCEEEEEEEEE
VDEPGESLVSGKLLADIARSLPNKPVQITTDPAKMDLVCGSARFTLQALPVDEYPDLPQM
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCEEEEEECCCCCCCCCCCC
PAATGTVDASVFSRAVAQVVVAAGRDELLPVFTGVRVEINGETLSLLATDRYRMALKEIT
CCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECEEEEECCCEEEEEECHHHHHHHHHHC
WNPSATDAEATALVPAKVINETARSMTSGEHVTMNLSSGDSGEGLVGFEGDGANGVRRMT
CCCCCCCCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHH
TRLLSGEFPKVRHLMDIKATRSVRARTDELINSVRRVSLVAERNTPLRMVINDDSVALSA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCEEEEE
ATGDQAQASEAIEAVVTNHVDGEPTITAAGFNPHYLSDALGALDTPYVHFSFTAPGKPCL
CCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCEE
VTGLNDFDGKPETDYRHVIMLMRLPS
EECCCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MEIRLERDVMAEAVAWAARSLPNRPTVPILAGLLVRAEGDSVVMSASDNETSAQITLSAQ
CCEEEHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEECCCEEEEECCCCCCEEEEEEEEE
VDEPGESLVSGKLLADIARSLPNKPVQITTDPAKMDLVCGSARFTLQALPVDEYPDLPQM
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCEEEEEECCCCCCCCCCCC
PAATGTVDASVFSRAVAQVVVAAGRDELLPVFTGVRVEINGETLSLLATDRYRMALKEIT
CCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECEEEEECCCEEEEEECHHHHHHHHHHC
WNPSATDAEATALVPAKVINETARSMTSGEHVTMNLSSGDSGEGLVGFEGDGANGVRRMT
CCCCCCCCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHH
TRLLSGEFPKVRHLMDIKATRSVRARTDELINSVRRVSLVAERNTPLRMVINDDSVALSA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCEEEEE
ATGDQAQASEAIEAVVTNHVDGEPTITAAGFNPHYLSDALGALDTPYVHFSFTAPGKPCL
CCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCEE
VTGLNDFDGKPETDYRHVIMLMRLPS
EECCCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1577691; 12000953 [H]