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The map label for this gene is pyrP [H]

Identifier: 50365473

GI number: 50365473

Start: 765064

End: 766458

Strand: Reverse

Name: pyrP [H]

Synonym: Mfl658

Alternate gene names: 50365473

Gene position: 766458-765064 (Counterclockwise)

Preceding gene: 50365474

Following gene: 50365472

Centisome position: 96.63

GC content: 31.61

Gene sequence:

>1395_bases
ATGAAAGAGTTAAAACAAGAAGAAATAGGTCTTGCTTTAGAGCCGAGAGAAAAGCCAAAAAGTTATGGACAATGATTTAT
ATTTTCTATTCAACATGTGTTTGCTATGTTTGGATCAACAGTGCTTGTTCCCATTATTATAAATGGACTTGCTAAAGAGG
TTGTAATGACTTCTTCAATGGCATTATTTTGTTCTGGTGTTGGAACATTAATATATATTGCTTTAACAAAAGCAAAAGTG
CCAATGTATTTAGGTAGTTCGTTTGCGTATATGACAGCGATCGGAATGAATTATCAAGCATATGGAAATTCAGTATTTGT
GGCTATAATGGTTGCTGGACTGATTTATATATTATTTGGGATATTAGTATATTATTTAGGTACTGGTTTTATTGAAAAAA
TATTTCCTGTAATTGTTATAGGACCTTTAATAATGATAATTGGATTAAGCGCAGCACCTTCTGCATTAGCTAATGCGGGT
ATAACAAGTGCAAAAAGTTGGAGTGATTCAGCAAAGGGATATGCACAATGAATTGCAGCACTAATAGCAGTATTTACTTT
TATGGTGACTATTATAGTAACATTGAAAGCTAAAGGGATAGCAAAAGTTATTCCGGTTATGATTGGAATATTATCAGGAT
TCGTTTTAAGTTTGATTATTCATTTTTCAATGAAAAATTCAACATTAATAGATACAACAAAAATAACAGATGTATCTCAG
TGAGAATGATATCCTTCATTCAAACCATTATGAAAACAAGAAGCCAAAAATATATTGCCAGCGATATTAGCAATATCACC
ATTAGCAATAATTGCAATGGCAGAACATATTGGCGATCATATAGCAATGGGATACATAACTAAAAAAGATTTTGTAAAAG
ACCCTGGTATACATAGAACACTTATTGCAGATGGTGTTTCTATAGTATTTGATGGTTTAGTTGGTGGCCCACCAAACACT
ACTTATGGTGAAAATACAGCGGTTATTGGTATGACAAAGGTTGCATCTGTGTGAGTAACTGGTGGAGCCGCAGTAATAGC
TATAATTTTATCATTTGTTGCACCAGTTAACCAAACAATATCAATGCTACCAGAGCCTGTAATGGGTGGTGTTGGAATGA
TTATGTTTGGATTTATAAGTATTAATGGTGTAAGAATAATGATAACAAGTAAAACAGATTTTATGAATATGAGAAATGTA
TTCATATCAGCTACAGTATTAGTAATTGGAGTTGTTCTTAGCGTATTAGGTGAAGGAATAGACATAGGTTCATTCAATTT
ACCTGGATTAGGGCTAGCTGCATTTATAGGTATAATTTTAAATTTAATATTACCGACAAAATTAAATGAAGGTTTTATGT
GCATAGAATGAATAAAATCTAAATTTAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTAAAAAATCCACCAATAATCGAAAACAAGTTTTTAACAACCAAATAATCGTCTTTTATAGACGATTATTTTTTTTGT
AAAAACGGAAAGAGGGGAAA

Downstream 100 bases:

>100_bases
AATAATATATATTGTATAATTCAAATAGAAAAGAGGATCATATGATAATAAAAAATGAATGATTTAATAAACATAAAAAT
GTTTTTTTAAATATGTTTGT

Product: xanthine/uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKELKQEEIGLALEPREKPKSYGQWFIFSIQHVFAMFGSTVLVPIIINGLAKEVVMTSSMALFCSGVGTLIYIALTKAKV
PMYLGSSFAYMTAIGMNYQAYGNSVFVAIMVAGLIYILFGILVYYLGTGFIEKIFPVIVIGPLIMIIGLSAAPSALANAG
ITSAKSWSDSAKGYAQWIAALIAVFTFMVTIIVTLKAKGIAKVIPVMIGILSGFVLSLIIHFSMKNSTLIDTTKITDVSQ
WEWYPSFKPLWKQEAKNILPAILAISPLAIIAMAEHIGDHIAMGYITKKDFVKDPGIHRTLIADGVSIVFDGLVGGPPNT
TYGENTAVIGMTKVASVWVTGGAAVIAIILSFVAPVNQTISMLPEPVMGGVGMIMFGFISINGVRIMITSKTDFMNMRNV
FISATVLVIGVVLSVLGEGIDIGSFNLPGLGLAAFIGIILNLILPTKLNEGFMCIEWIKSKFKK

Sequences:

>Translated_464_residues
MKELKQEEIGLALEPREKPKSYGQ*FIFSIQHVFAMFGSTVLVPIIINGLAKEVVMTSSMALFCSGVGTLIYIALTKAKV
PMYLGSSFAYMTAIGMNYQAYGNSVFVAIMVAGLIYILFGILVYYLGTGFIEKIFPVIVIGPLIMIIGLSAAPSALANAG
ITSAKSWSDSAKGYAQ*IAALIAVFTFMVTIIVTLKAKGIAKVIPVMIGILSGFVLSLIIHFSMKNSTLIDTTKITDVSQ
*E*YPSFKPL*KQEAKNILPAILAISPLAIIAMAEHIGDHIAMGYITKKDFVKDPGIHRTLIADGVSIVFDGLVGGPPNT
TYGENTAVIGMTKVASV*VTGGAAVIAIILSFVAPVNQTISMLPEPVMGGVGMIMFGFISINGVRIMITSKTDFMNMRNV
FISATVLVIGVVLSVLGEGIDIGSFNLPGLGLAAFIGIILNLILPTKLNEGFMCIE*IKSKFKK
>Mature_464_residues
MKELKQEEIGLALEPREKPKSYGQ*FIFSIQHVFAMFGSTVLVPIIINGLAKEVVMTSSMALFCSGVGTLIYIALTKAKV
PMYLGSSFAYMTAIGMNYQAYGNSVFVAIMVAGLIYILFGILVYYLGTGFIEKIFPVIVIGPLIMIIGLSAAPSALANAG
ITSAKSWSDSAKGYAQ*IAALIAVFTFMVTIIVTLKAKGIAKVIPVMIGILSGFVLSLIIHFSMKNSTLIDTTKITDVSQ
*E*YPSFKPL*KQEAKNILPAILAISPLAIIAMAEHIGDHIAMGYITKKDFVKDPGIHRTLIADGVSIVFDGLVGGPPNT
TYGENTAVIGMTKVASV*VTGGAAVIAIILSFVAPVNQTISMLPEPVMGGVGMIMFGFISINGVRIMITSKTDFMNMRNV
FISATVLVIGVVLSVLGEGIDIGSFNLPGLGLAAFIGIILNLILPTKLNEGFMCIE*IKSKFKK

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=459, Percent_Identity=22.8758169934641, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI40316845, Length=459, Percent_Identity=22.8758169934641, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788843, Length=423, Percent_Identity=35.6973995271868, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87081818, Length=442, Percent_Identity=29.8642533936652, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87082181, Length=426, Percent_Identity=28.6384976525822, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1790087, Length=477, Percent_Identity=27.0440251572327, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082178, Length=429, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 48490; Mature: 48490

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCC
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1709162; 8206849; 9384377 [H]