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The map label for this gene is gltX [H]

Identifier: 50365466

GI number: 50365466

Start: 756205

End: 757653

Strand: Reverse

Name: gltX [H]

Synonym: Mfl651

Alternate gene names: 50365466

Gene position: 757653-756205 (Counterclockwise)

Preceding gene: 50365467

Following gene: 50365465

Centisome position: 95.52

GC content: 26.85

Gene sequence:

>1449_bases
ATGACTAAGTTCAGATTAAGATATGCACCATCGCCAACTGGTTTCTTACACATTGGAAACACAAGAACAGCTTTAATGAA
TTATTTATTTGCTAAACATTATAATGGTGATTTTATTGTAAGAATAGAAGATACTGATTTAGAAAGAAATGTTGAAGGAG
CAATTGAATCACAATTTGAAAACTTAAATTGGCTTGGAATAAATGCAGATGAATCATTTTTAAAACCTGGTGAAGAGAAG
TATGGTAAGTACATGCAATCTCAAAAATTTGGTAGATATCAAGAACTTGCTGAAAAACTTATTTCAATAAAAAAAGCATA
TCGTTGTTTTTGTACAACTGAAGAATTGGAAAAAGATTATGAAGATCAAGTTGCAAAAGGCATAGTTGCTACTAAGTACT
CTGGGAAGTGCTCAAGATTAAGTGAAAGTGAAATTGAGTCAAATTTAAAATCGAATAAAGATTTTTCAATTAGATTTTTA
GTTCCTGAAACTACTTTGAATATTAAAGACTTTATAAAAGGTGAAATTACATTTGATTCTAAGGAGTTAGGAGATTTTGT
TATTTTAAAAACTAATAAAATAGCGACATATAACTTTGCTGTTGTTGTTGATGATTTTGACATGGAAATTTCTCATGTTT
TAAGAGGTGAAGAACATATTTCAAATACTCCAAGACAAATTTTAATTTATCAAGCTTTTGGATGAGAAACACCTCAATTT
GGACATATGTCATTAATTGTTGATTCTACAGGTAAAAAATTGTCAAAAAGAAGTGGTAATGCATTATTTTTCATTGAGCA
GTATAAAAATCAAGGTTATTTACCAGAGGCAATGTTTAATTACATTTCTTTATTAGGTTGATCACCAATTGGAGAAAAAG
AATTGTTGACTAAGCAAGAATTAATTTCAATGTTTGATGATAAAAGATTTAGTAAATCTCCTTCAACATTTGATATGACA
AAAATGAAGTGAATAAATTCTCAATATATGAAAGCCTTATCTGAAGAAGAATATTTAGAATTTACTAAAAAATACATTAA
TACTGAAATGTTTAATACTAAAGAGAAATCTGAAGATTGATTAAATCAAGTTTTACTTTTATTTAAAAAAGAATTAGAGT
TTGCTGATCAAATCAATAATCATTTAAATATATTCTTTAATGAAGTTGATGTTACAAGTGAAACAATTGAAATATTGAAA
ACAATTGAAGAGCATGAAAGTGTCATTAAAGAATTTGAAACTCAAATATCTACTTTAAACGAATGAGAAATCGAAAATAT
TAAATCTTTAATTAAATTAGTTAGCGAAAATACTGGTAAAAAAGGTAAAGATTTATTTATGCCTATCAGAATTGCTTCTT
CAAGCTCTGAACATGGTCCTTCACTAGCTGATGTTATTTATTTATTAGGTAAAGATAAGGTTTTAGCAAACATAGCTAAG
GTTAAATAG

Upstream 100 bases:

>100_bases
AAAAAGAAAACTTAAAAAAAGCGTCAAAAGATAAAAAGAAAACAAACAAATAAAAAAATTGATAAAATATATAAAGTTTG
AAAATTACAAAGGAGAATAC

Downstream 100 bases:

>100_bases
GTAATTATTATGGAAAAAGTTTTTAGAGATAGTATTCACGGTGACATATTTATTCATGATGAGATTTTTATGGAAATAAT
TAATTCACCAGAAATGCAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTKFRLRYAPSPTGFLHIGNTRTALMNYLFAKHYNGDFIVRIEDTDLERNVEGAIESQFENLNWLGINADESFLKPGEEK
YGKYMQSQKFGRYQELAEKLISIKKAYRCFCTTEELEKDYEDQVAKGIVATKYSGKCSRLSESEIESNLKSNKDFSIRFL
VPETTLNIKDFIKGEITFDSKELGDFVILKTNKIATYNFAVVVDDFDMEISHVLRGEEHISNTPRQILIYQAFGWETPQF
GHMSLIVDSTGKKLSKRSGNALFFIEQYKNQGYLPEAMFNYISLLGWSPIGEKELLTKQELISMFDDKRFSKSPSTFDMT
KMKWINSQYMKALSEEEYLEFTKKYINTEMFNTKEKSEDWLNQVLLLFKKELEFADQINNHLNIFFNEVDVTSETIEILK
TIEEHESVIKEFETQISTLNEWEIENIKSLIKLVSENTGKKGKDLFMPIRIASSSSEHGPSLADVIYLLGKDKVLANIAK
VK

Sequences:

>Translated_482_residues
MTKFRLRYAPSPTGFLHIGNTRTALMNYLFAKHYNGDFIVRIEDTDLERNVEGAIESQFENLNWLGINADESFLKPGEEK
YGKYMQSQKFGRYQELAEKLISIKKAYRCFCTTEELEKDYEDQVAKGIVATKYSGKCSRLSESEIESNLKSNKDFSIRFL
VPETTLNIKDFIKGEITFDSKELGDFVILKTNKIATYNFAVVVDDFDMEISHVLRGEEHISNTPRQILIYQAFG*ETPQF
GHMSLIVDSTGKKLSKRSGNALFFIEQYKNQGYLPEAMFNYISLLG*SPIGEKELLTKQELISMFDDKRFSKSPSTFDMT
KMK*INSQYMKALSEEEYLEFTKKYINTEMFNTKEKSED*LNQVLLLFKKELEFADQINNHLNIFFNEVDVTSETIEILK
TIEEHESVIKEFETQISTLNE*EIENIKSLIKLVSENTGKKGKDLFMPIRIASSSSEHGPSLADVIYLLGKDKVLANIAK
VK
>Mature_481_residues
TKFRLRYAPSPTGFLHIGNTRTALMNYLFAKHYNGDFIVRIEDTDLERNVEGAIESQFENLNWLGINADESFLKPGEEKY
GKYMQSQKFGRYQELAEKLISIKKAYRCFCTTEELEKDYEDQVAKGIVATKYSGKCSRLSESEIESNLKSNKDFSIRFLV
PETTLNIKDFIKGEITFDSKELGDFVILKTNKIATYNFAVVVDDFDMEISHVLRGEEHISNTPRQILIYQAFG*ETPQFG
HMSLIVDSTGKKLSKRSGNALFFIEQYKNQGYLPEAMFNYISLLG*SPIGEKELLTKQELISMFDDKRFSKSPSTFDMTK
MK*INSQYMKALSEEEYLEFTKKYINTEMFNTKEKSED*LNQVLLLFKKELEFADQINNHLNIFFNEVDVTSETIEILKT
IEEHESVIKEFETQISTLNE*EIENIKSLIKLVSENTGKKGKDLFMPIRIASSSSEHGPSLADVIYLLGKDKVLANIAKV
K

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=31.5789473684211, Blast_Score=248, Evalue=8e-66,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=35.6107660455487, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI226510925, Length=258, Percent_Identity=32.9457364341085, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=472, Percent_Identity=33.0508474576271, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324540, Length=486, Percent_Identity=32.9218106995885, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324742, Length=318, Percent_Identity=27.3584905660377, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321192, Length=251, Percent_Identity=25.8964143426295, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=32.5252525252525, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54784; Mature: 54653

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFRLRYAPSPTGFLHIGNTRTALMNYLFAKHYNGDFIVRIEDTDLERNVEGAIESQFE
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHC
NLNWLGINADESFLKPGEEKYGKYMQSQKFGRYQELAEKLISIKKAYRCFCTTEELEKDY
CCEEEEECCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EDQVAKGIVATKYSGKCSRLSESEIESNLKSNKDFSIRFLVPETTLNIKDFIKGEITFDS
HHHHHCCEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCEEEECC
KELGDFVILKTNKIATYNFAVVVDDFDMEISHVLRGEEHISNTPRQILIYQAFGETPQFG
HHHCCEEEEEECEEEEEEEEEEEECCCHHHHHHHCCCHHHCCCCCEEEEEEECCCCCCCC
HMSLIVDSTGKKLSKRSGNALFFIEQYKNQGYLPEAMFNYISLLGSPIGEKELLTKQELI
CEEEEEECCCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
SMFDDKRFSKSPSTFDMTKMKINSQYMKALSEEEYLEFTKKYINTEMFNTKEKSEDLNQV
HHHHCCCCCCCCCCCCHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LLLFKKELEFADQINNHLNIFFNEVDVTSETIEILKTIEEHESVIKEFETQISTLNEEIE
HHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKSLIKLVSENTGKKGKDLFMPIRIASSSSEHGPSLADVIYLLGKDKVLANIAKVK
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
TKFRLRYAPSPTGFLHIGNTRTALMNYLFAKHYNGDFIVRIEDTDLERNVEGAIESQFE
CCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHC
NLNWLGINADESFLKPGEEKYGKYMQSQKFGRYQELAEKLISIKKAYRCFCTTEELEKDY
CCEEEEECCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EDQVAKGIVATKYSGKCSRLSESEIESNLKSNKDFSIRFLVPETTLNIKDFIKGEITFDS
HHHHHCCEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCEEEECC
KELGDFVILKTNKIATYNFAVVVDDFDMEISHVLRGEEHISNTPRQILIYQAFGETPQFG
HHHCCEEEEEECEEEEEEEEEEEECCCHHHHHHHCCCHHHCCCCCEEEEEEECCCCCCCC
HMSLIVDSTGKKLSKRSGNALFFIEQYKNQGYLPEAMFNYISLLGSPIGEKELLTKQELI
CEEEEEECCCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
SMFDDKRFSKSPSTFDMTKMKINSQYMKALSEEEYLEFTKKYINTEMFNTKEKSEDLNQV
HHHHCCCCCCCCCCCCHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LLLFKKELEFADQINNHLNIFFNEVDVTSETIEILKTIEEHESVIKEFETQISTLNEEIE
HHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKSLIKLVSENTGKKGKDLFMPIRIASSSSEHGPSLADVIYLLGKDKVLANIAKVK
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA